Comparative Analysis of the DECODE Systems in Hematopoiesis and Oxidative Stress Response

• Project/Area Number: 17054028
• Research Category: Grant-in-Aid for Scientific Research on Priority Areas
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: Tohoku University
• Principal Investigator: IGARASHI Kazuhiko Tohoku University, 大学院・医学系研究科, 教授 (00250738)
• Co-Investigator(Kenkyū-buntansha): MUTO Akihiko 東北大学, 大学院・医学系研究科, 講師 (80343292) ; 太田 力 国立がんセンター研究所, 疾病ゲノムセンター, 室長 (10290892)
• Project Period (FY): 2005 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥141,200,000 (Direct Cost: ¥141,200,000); Fiscal Year 2009: ¥28,400,000 (Direct Cost: ¥28,400,000); Fiscal Year 2008: ¥28,400,000 (Direct Cost: ¥28,400,000); Fiscal Year 2007: ¥28,400,000 (Direct Cost: ¥28,400,000); Fiscal Year 2006: ¥28,400,000 (Direct Cost: ¥28,400,000); Fiscal Year 2005: ¥27,600,000 (Direct Cost: ¥27,600,000)
• Keywords: Bach1 / Bach2 / 転写因子 / クロマチン / 発現制御

Research Abstract

DECODE complexes of Bach1, Bach2, and MafK were purified and characterized by using mass spectrometry analysis. Bach1 was found to interact with the tumor suppressor p53 and to inhibit cellular senescence induced by oxidative stress. Bach1 recruits histone deacetylase 1 to some of the p53 target genes and promotes histone deacetylation. In addition, a DECODE gene circuit for the class switch recombination of antibody genes has been identified.

Research Products

• [Journal Article] Bach1-dependent and -independent regulation of heme oxygenase-1 in keratinocytes. (2010)
  • Author(s): Okada, S., Muto, A., Ogawa, E., Nakanome, A., Katoh, Y., Ikawa, S., Aiba, S., Igarashi, K., Okuyama, R
  • Journal Title: J. Biol. Chem (in press)
  • Peer Reviewed
• [Journal Article] Characterization of the cancer chemopreventive NRF2-dependent gene battery in human keratinocytes: demonstration that the KEAP1-NRF2 pathway, and not the BACH1-NRF2 pathway, controls cytoprotection against electrophiles as well as redox-cycling compounds. (2009)
  • Author(s): MacLeod, A. K, McMahon, M., Plummer, S.M., Higgins, L.G., Penning, T.M., Igarashi, K., Hayes JD
  • Journal Title: Carcinogenesis 30 Pages: 1571-1580
  • Peer Reviewed
• [Journal Article] Crystal structure of the Bach1 BTB domain and its regulation of homodimerization. (2009)
  • Author(s): Ito, N., Watanabe-Matsui, M., Igarashi, K., Murayama, K
  • Journal Title: Genes Cells 14 Pages: 1365-2443
  • Peer Reviewed
• [Journal Article] Charactrization of the canoer chemopreventive NRF2-dependent gene battery in human keratinocytes : demonstration that the KEAP1-NRF2 pathway, and not the BACH1-NRF2 pathway, controls cytoprotection against electrophiles as well as redox-cycling compounds (2009)
  • Author(s): MacLeod, A.K., et al
  • Journal Title: Carcinogenesis 30 Pages: 1571-1580
  • Peer Reviewed
• [Journal Article] Crystal structure of the Bach1 BTB domain and its regulation of homodimerization (2009)
  • Author(s): Ito, N., et.al.
  • Journal Title: Genes Cells 14 Pages: 1365-2443
  • Peer Reviewed
• [Journal Article] Genetic ablation of the Bach1 gene reduces hyperoxic lung injury in mice : role of IL-6 (2009)
  • Author(s): Tanimoto, T., et al.
  • Journal Title: Free Radic Biol Med 46 Pages: 1119-1126
  • Peer Reviewed
• [Journal Article] Bach1 deficiency ameliorabes hepatic injury in a ddmouse model (2009)
  • Author(s): Iida, A., et.al.
  • Journal Title: Tohoku J Exp Med. 217 Pages: 223-229
  • Peer Reviewed
• [Journal Article] Genetic ablation of transcription repressor Bach1 reduces neural tissue damage and improves locomotor function after spinal cord injuny in mice (2009)
  • Author(s): Kanno, H., et al.
  • Journal Title: J.Neurotrauma 26 Pages: 31-39
  • Peer Reviewed
• [Journal Article] Crystal structure of the Bachl BTB domain and its regulation of homodimerization (2009)
  • Author(s): Ito, N, et.al.
  • Journal Title: Genes Cells 14 Pages: 1365-2443
  • Peer Reviewed
• [Journal Article] Bach1 inhibits oxidative stress-induced cellular senescence by impeding p53 function on chromatin. (2008)
  • Author(s): Dohi, Y., Ikura, T., Hoshikawa, Y., Katoh, Y., Ota, K., Nakanome, A., Muto, A., Omura, S., Ohta, T., Ito, A., Yoshida, M., Noda, T., Igarashi, K
  • Journal Title: Nature Struct. Mol. Biol 15 Pages: 1246-1254
  • Peer Reviewed
• [Journal Article] Regulation of the plasma cell transcription factor Blimp-1 gene by Bach2 and Bcl6. (2008)
  • Author(s): Ochiai, K., Muto, A., Tanaka, H., Takahashi, S., Igarashi, K
  • Journal Title: Int. Immunol 20 Pages: 453-460
  • NAID: 10021240257
  • Peer Reviewed
• [Journal Article] Bachl inhibits oxidative stress-induced cellular senescence by impeding p53 function or chromatin (2008)
  • Author(s): Dohi, Y, et.al.
  • Journal Title: Nature Struct. Mol. Biol 15 Pages: 1246-1254
  • Peer Reviewed
• [Journal Article] Regulation of the plasma cell transcription factor Blimp-1 gene by Bach2 and Bc16 (2008)
  • Author(s): Ochiai, K, et.al.
  • Journal Title: Int. Immunol 20 Pages: 453-460
  • Peer Reviewed
• [Journal Article] Regulation of heme oxygenase-1 by transcription factor Bachl in the mouse brain (2008)
  • Author(s): Sakoda, E, et.al.
  • Journal Title: Neuroscience Lett 440 Pages: 160-165
  • Peer Reviewed
• [Journal Article] The mobility of Bach2 nuclear foci is regulated by SUMO-1 modification (2008)
  • Author(s): Kono, K, et.al.
  • Journal Title: Exp. Cell Res 314 Pages: 903-913
  • Peer Reviewed
• [Journal Article] Regulation of the plasma cell transcription factor Blimp-1 gene by Bach2 and Bc16. (2008)
  • Author(s): Ochiai, K., et. al.
  • Journal Title: Int. Immunol. 20 Pages: 453-460
  • Peer Reviewed
• [Journal Article] Heme induces ubiquitination and degradation of the transcription factor Bach1. (2007)
  • Author(s): Zenke-Kawasaki, Y., Dohi, Y., Katoh, Y., Ikura, T., Ikura, M., Asahara, T., Tokunaga, F., Iwai, K., Igarashi, K
  • Journal Title: Mol. Cell. Biol 27 Pages: 6962-6971
  • Peer Reviewed
• [Journal Article] Corepressor SMRT and class II histone deacetylases promote Bach2 nuclear retention and formation of nuclear foci that are responsible for local transcriptional repression. (2007)
  • Author(s): Hoshino, H., et. al.
  • Journal Title: J. Biochem. 141 Pages: 719-727
  • Peer Reviewed
• [Journal Article] Heme induces ubiquitination and degradation of the transcription factor Bach1. (2007)
  • Author(s): Zenke-Kawasaki, Y., et. al.
  • Journal Title: Mol. Cell. Biol. 27 Pages: 6962-6971
  • Peer Reviewed
• [Journal Article] Nuclear positioning of the BACH2 gene in BCR-ABL positive leukemia cells (2007)
  • Author(s): Ono, A.et al.
  • Journal Title: Genes, Chromosomes ＆ Cancer. 46 Pages: 67-74
• [Journal Article] Architecture and dynamics of the transcription factor network that regulates B-to-plasma cell differentiation. (2007)
  • Author(s): Igarashi, K. et al.
  • Journal Title: J. Biochem. (in press)
  • NAID: 10020098307
• [Journal Article] Bcr-Abl signaling through the PI-3/S6 kinase pathway inhibits nuclear translocation of the transcription factor Bach2 which represses the anti-apoptotic factor Hemeoxygenase-1. (2007)
  • Author(s): Yoshida, C. et al.
  • Journal Title: Blood. 109 Pages: 1211-1219
• [Journal Article] Corepressor SMRT and class II histone deacetylases promote Bach2 nuclear retention and formation of nuclear foci that are responsible for local transcriptional repression. (2007)
  • Author(s): Hoshino, H. et al.
  • Journal Title: J. Biochem. (in press)
• [Journal Article] Plasmacytic transcription factor Blimp-1 is repressed by Bach2 in B cells. (2006)
  • Author(s): Ochiai, K., Katoh, Y., Ikura, T., Hoshikawa, Y., Noda, T., Karasuyama, H., Tashiro, S., Muto, A., Igarashi, K
  • Journal Title: J. Biol. Chem 281 Pages: 38226-38234
  • Peer Reviewed
• [Journal Article] Plasmacytic transcription factor Blimp-1 is repressed by Bach2 in B cells. (2006)
  • Author(s): Ochiai, K. et al.
  • Journal Title: J. Biol. Chem. 281 Pages: 38226-38234
• [Journal Article] Differential gene expression profiling between wild-type and ALAS2-null erythroblasts : Identification of novel heme-regulated genes. (2006)
  • Author(s): Fujiwara, T. et al.
  • Journal Title: Biochem. Biophys. Res. Commun. 340 Pages: 105-110
• [Journal Article] Heme oxygenase-1 gene enhancer manifests silencing activity in a chromatin environment prior to oxidative stress. (2006)
  • Author(s): Dohi, Y. et al.
  • Journal Title: Antioxid. Redox Signal. 8 Pages: 60-67
• [Journal Article] The heme-Bach1 pathway in the regulation of oxidative stress response and erythroid differentiation. (2006)
  • Author(s): Igarashi, K., Sun, J.
  • Journal Title: Antioxid. Redox Signal. 8 Pages: 107-118
• [Journal Article] Effects of genetic ablation of bach1 upon smooth muscle cell proliferation and atherosclerosis after cuff injury. (2005)
  • Author(s): Omura, S., Suzuki, H., Toyofuku, M., Ozono, R., Kohno, N., Igarashi, K
  • Journal Title: Genes Cells 10 Pages: 277-285
  • Peer Reviewed
• [Journal Article] Transgenic expression of Bach1 transcription factor results in megakaryocytic impairment. (2005)
  • Author(s): Toki, T. et al.
  • Journal Title: Blood 105 Pages: 3100-3108
• [Journal Article] Effects of genetic ablation of bach1 upon smooth muscle cell proliferation and atherosclerosis after cuff injury. (2005)
  • Author(s): Omura, S. et al.
  • Journal Title: Genes Cells 10 Pages: 277-285
• [Journal Article] Dynamic cytoplasmic anchoring of the transcription factor Bach1 by intracellular hyaluronic acid binding protein IHABP. (2005)
  • Author(s): Yamasaki, C. et al.
  • Journal Title: J. Biochem. 136 Pages: 287-296
• [Presentation] 核内メチル化反応を支えるタンパク質ネットワーク (2010)
  • Author(s): 五十嵐和彦
  • Organizer: 日本薬学会シンポジウム
  • Place of Presentation: 岡山コンベンションセンター
  • Year and Date: 2010-03-29
• [Presentation] Dense co-regulators network for HO-1 gene expression 6th International Congress on Hemeoxygenases (2009)
  • Author(s): 五十嵐和彦
  • Organizer: Heme oxygenases in Biology & Medicine
  • Place of Presentation: マイアミビーチリゾートアンドスパ(米国)
  • Year and Date: 2009-10-01
• [Presentation] Cross-talk between Bach1, NF-kB, and IL-6 in epithelial-mesenchymal transition (2009)
  • Author(s): Brydun, A., Nakanome A., Matsumoto, M., Ota, K., Dohi, Y., Igarashi, K
  • Organizer: American Association for Cancer Reserch International Conference on Frontiers in Basic Cancer Research
  • Place of Presentation: Boston, USA
• [Presentation] Regulation of cell proliferation by the heme-Bach1 pathway (2006)
  • Author(s): Kazuhiko Igarashi
  • Organizer: Gordon Research Conference on Tetrapyrroles, Chemistry and Biology of
  • Place of Presentation: Newport, RI, USA
• [Presentation] Heme-mediated gene regulation in mammalian cells (2005)
  • Author(s): K. Igarashi, Y. Zenke, Y. Dohi
  • Organizer: Cold Spring Harbor Laboratory Meeting "Mechanisms of eukaryotic transcription"
  • Place of Presentation: マイアミ. USA