| Project/Area Number |
17054033
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research on Priority Areas
|
| Allocation Type | Single-year Grants |
|---|
| Review Section |
Biological Sciences
|
|---|
| Research Institution | Yokohama City University |
|---|
Principal Investigator |
OGATA Kazuhiro Yokohama City University, 医学研究科, 教授 (90260330)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
佐藤 光 横浜市立大学, 医学研究科, 助教 (90300962)
椎名 政昭 横浜市立大学, 医学研究科, 助教 (30347299)
浜田 恵輔 横浜市立大学, 医学研究科, 助教 (00344052)
|
|---|
| Co-Investigator(Renkei-kenkyūsha) |
SHIINA Masaaki 横浜市立大学, 医学部, 助教 (30347299)
HAMADA Keisuke 横浜市立大学, 医学部, 助教 (00344052)
SATO Ko 横浜市立大学, 医学部, 助教 (90300962)
|
|---|
| Project Period (FY) |
2005 – 2009
|
| Project Status |
Completed (Fiscal Year 2009)
|
| Budget Amount *help |
¥105,600,000 (Direct Cost: ¥105,600,000)
Fiscal Year 2009: ¥21,000,000 (Direct Cost: ¥21,000,000)
Fiscal Year 2008: ¥21,000,000 (Direct Cost: ¥21,000,000)
Fiscal Year 2007: ¥21,000,000 (Direct Cost: ¥21,000,000)
Fiscal Year 2006: ¥21,000,000 (Direct Cost: ¥21,000,000)
Fiscal Year 2005: ¥21,600,000 (Direct Cost: ¥21,600,000)
|
|---|
| Keywords | 転写因子 / タンパク質-核酸複合体 / Ets1,Runx1,エンハンサー / 化学修飾 / 連携研究者 / 転写制御因子 / エンハンソソーム / Runx1 / Ets1 / ヒストンシャペロン / クロマチンリモデリング / エンハンセオソーム / ヒストンシヤペロン / リン酸化 / MeCP2 |
|---|
| Research Abstract |
Multiple transcription factors (TFs) regulate transcription by forming TFs-DNA complexes. A cell signaling causes chemical modification of transcription factors, which modulates transcriptional activities. Besides, effect of chemical modification of transcription factors on their activity in the context of TFs-DNA complexes, however, is largely unknown. In this sturdy, we showed that the phosphorylation of a transcription factor Ets1 "differentially" modulates Ets1-containing TFs-DNA complexes. In addition, we clarified the underlying mechanism by which the selective modulation of Ets1-containing TFs-DNA complexes is achieved using NMR analyses of allosteric regulation of Runx1 by CBFβ and crystallographic analyses of multiprotein-DNA complex comprised of Ets1, Runx1 and CBFβ formed on the TCRα gene enhancer.
|
