Clarification of the energy and signal transduction of bacterial flagellar motor using hybrid motor
Project/Area Number |
17076005
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Research Category |
Grant-in-Aid for Scientific Research on Priority Areas
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Allocation Type | Single-year Grants |
Review Section |
Science and Engineering
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Research Institution | Tohoku University (2006-2009) Nagoya University (2005) |
Principal Investigator |
ISHIJIMA Akihiko Tohoku University, 多元物質科学研究所, 教授 (80301216)
|
Co-Investigator(Kenkyū-buntansha) |
KAWAGISHI Ikuro 法政大学, 工学部, 教授 (80234037)
HOMMA Michio 名古屋大学, 理学研究科, 教授 (50209342)
|
Project Period (FY) |
2005 – 2009
|
Project Status |
Completed (Fiscal Year 2009)
|
Budget Amount *help |
¥82,500,000 (Direct Cost: ¥82,500,000)
Fiscal Year 2009: ¥15,200,000 (Direct Cost: ¥15,200,000)
Fiscal Year 2008: ¥15,400,000 (Direct Cost: ¥15,400,000)
Fiscal Year 2007: ¥15,500,000 (Direct Cost: ¥15,500,000)
Fiscal Year 2006: ¥17,400,000 (Direct Cost: ¥17,400,000)
Fiscal Year 2005: ¥19,000,000 (Direct Cost: ¥19,000,000)
|
Keywords | バクテリア / べん毛モーター / ナノ計測 / イメージング / 回転計測 / GFP / 走化性 / モータータンパク / レセプター / 細胞内局在 / モーター / 1分子計測 / イオン / キメラ菌体 / イオンモーター / ステップ計測 |
Research Abstract |
The bacterial flagellar motor is a rotary molecular machine that rotates the helical filaments that propel many species of swimming bacteria. The rotor is a set of rings up to 45nm in diameter in the cytoplasmic membrane3 ; the stator contains about ten torque generating units anchored to the cell wall at the perimeter of the rotor4, 5. The free-energy source for the motor is an inward directed electrochemical gradient of ions across the cytoplasmic membrane, the protonmotive force or sodium-motive force for H1-driven and Na1-driven motors, respectively. Here we demonstrate a stepping motion of a Na1-driven chimaeric flagellar motor in Escherichia coli6 at low sodium-motive force and with controlled expression of a small number of torque-generating units. We observe 26 steps per revolution, which is consistent with the periodicity of the ring of FliG protein, the proposed site of torque generation on the rotor7, 8. Backwards steps despite the absence of the flagellar switching protein CheY indicate a small change in free energy per step, similar to that of a single ion transit.
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Report
(6 results)
Research Products
(145 results)
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[Journal Article] Adhesion Effects of a Guanidinium Ion Appended Dendritic"Molecular Glue"on the ATP-Driven Sliding Motion of Actomyosin2010
Author(s)
Okuro, K., Kinbara, K., Takeda, K., Inoue, Y., Ishijima, A., Aida, T.
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Journal Title
Angew Chem Int Ed Engl (In press)
Related Report
Peer Reviewed
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[Journal Article] Thermosensing function of the Escherichia coli redox sensor Aer2010
Author(s)
Nishiyama, S., Ohno, S., Ohta, N., Inoue, Y., Fukuoka, H., Ishijima, A., kawagishi, I.
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Journal Title
J.Bacteriol. 192
Pages: 1740-1743
Related Report
Peer Reviewed
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[Journal Article] Torque-speed relationships of Na+-driven chimeric flagellar motors in Escherichia coli2008
Author(s)
Inoue, Y., Lo, C.J., Fukuoka, H., Takahashi, H., Sowa, Y., Pilizota, T., Wadhams, G.H., Homma, M., Berry, R.M., Ishijima, A.
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Journal Title
J Mol Biol 376
Pages: 1251-1259
Related Report
Peer Reviewed
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[Journal Article] Systematic Cys mutagenesis of FlgI, the flagellar P-ring component of Escherichia coli2008
Author(s)
Hizukuri, Y., Kojima, S., Yakushi, T., Kawagishi, I., Homma, M.
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Journal Title
Microbiology 154
Pages: 810-817
Related Report
Peer Reviewed
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[Journal Article] Torque-speed relationships of Na^+-driyen chimeric flagellar motors in Escherichia coli2008
Author(s)
Inoue, Y., Lo, C. J., Fukuoka, H., Takahashi , H., Sowa, Y., Pilizota, T., Wadhams, G. H Homma, M., Berry, R. M. & Ishijima, A
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Journal Title
J Mol Biol 376
Pages: 1251-1259
Related Report
Peer Reviewed
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[Journal Article] Systematic Cys mutagenesis of FlgI, the flagellar P-ring component of Escherichia coli2008
Author(s)
Hizukuri, Y., Kojima, S., Yakus hi, T., Kawagishi, I., and Homma, M
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Journal Title
Microbiology 154
Pages: 810-817
Related Report
Peer Reviewed
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[Journal Article] Direct observation of steps in rotation of the bacterial flagellar motor2005
Author(s)
Sowa, Y., Rowe, A., Leake, M., Yakushi, T., Homma, M., Ishijima, A., Berry, R.
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Journal Title
Nature 437
Pages: 916-919
Related Report
Peer Reviewed
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[Journal Article] Formation and maintenance of tubular membrane projections require mechanical force, but their elongation and shortening do not require additional force.2005
Author(s)
Inaba, T., Ishijima, A., Honda, M., Nomura, F., Takiguchi, K., Hotani, H.
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Journal Title
Journal of Molecular Biology 348
Pages: 325-333
Related Report
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[Journal Article] Direct observation of steps in rotation of the bacterial flagellar motor.2005
Author(s)
Sowa, Y., Rowe, A., Leake, M., Yakushi, T., Homma, M., Ishijima, A., Berry, R.
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Journal Title
Nature 437
Pages: 916-919
Related Report
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[Journal Article] Only one of the five CheY homologs of Vibrio cholerae directly switches flagellar rotation.2005
Author(s)
Hyakutake, A., Homma, M., Austin, M.J., Boin, M.A., Hase, C.C., Kawagishi, I.
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Journal Title
J.Bacteriol. 187
Pages: 8403-8410
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[Presentation] コレラ菌走化性類似情報伝達因子の局在解析2009
Author(s)
蛯沢達朗, Hiremath, G., 百武晃宏, 鈴木大介, 西山宗一郎, 本間道夫, 川岸郁朗
Organizer
第15回べん毛研究交流会
Place of Presentation
仙台市(秋保グランドホテル)
Year and Date
2009-03-11
Related Report
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[Presentation] Thermosensing function of Aer, a redox sensor of E. coli2009
Author(s)
Nishiyama, S., Ohno, S. N. Ohta, N., Inoue, Y., Fukuoka, H., Ishijima, A., Kawagishi, I.
Organizer
BLAST X (Bacterial Locomotion and Signal Transduction X)
Place of Presentation
Cuernaraca. Mexico
Year and Date
2009-01-20
Related Report
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[Presentation] Localization patterns of the histidine kinases in an Escherichia coli cell2009
Author(s)
Inaba, T., Banno, S., Sawaki, H., Yamakawa, A., Yoshimoto, M., Homma, M., Kawagishi, I
Organizer
BLAST X (Bacterial Locomotion and Signal Transduction X)
Place of Presentation
Cuernaraca, Mexico
Year and Date
2009-01-20
Related Report
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[Presentation] Role of the HAMP domain of the bacterial chemoreceptor in signaling and subcellular localization2008
Author(s)
Ohta, N., Irieda, H., Banno, S., Homma, M., Kawagishi, I
Organizer
JSPS-DST Asian Academic Seminar 2008
Place of Presentation
Bangalore, India
Year and Date
2008-12-28
Related Report
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[Presentation] Localization control of components of the chemotaxis-related signalipg systems in Vibrio cholerae2008
Author(s)
Hiremath, G., Ebisawa, T., Hya kutake, A., Banno, S., Nishiyama, S., Kawagishi, I
Organizer
JSPS-DST Asian Academic Seminar 2008
Place of Presentation
Bangalore, India
Year and Date
2008-12-28
Related Report
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[Presentation] 大腸菌全ヒスチジンキナーゼの細胞内局在2008
Author(s)
澤木浩之, 坂野聡美, 山川明来子, 吉本誠之, 稲葉岳彦, 本間道夫, 川岸郁朗
Organizer
2008年ゲノム微生物学会若手の会
Place of Presentation
東京都(八王子セミナーハウス)
Year and Date
2008-11-06
Related Report
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