Real-World Modeling of Self-Emergent Active Cell
| Project/Area Number |
17076007
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| Research Category |
Grant-in-Aid for Scientific Research on Priority Areas
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| Allocation Type | Single-year Grants |
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| Review Section |
Science and Engineering
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| Research Institution | Kyoto University |
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Principal Investigator |
吉川 研一 Kyoto University, 大学院・理学研究科, 教授 (80110823)
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| Co-Investigator(Kenkyū-buntansha) |
SETO Hideki 京都大学, 大学院・理学研究科, 准教授 (60216546)
KITATAHA Hiroyuki 京都大学, 大学院・理学研究科, 助教 (20378532)
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| Project Period (FY) |
2005 – 2009
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| Project Status |
Completed (Fiscal Year 2009)
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| Budget Amount *help |
¥87,300,000 (Direct Cost: ¥87,300,000)
Fiscal Year 2009: ¥15,000,000 (Direct Cost: ¥15,000,000)
Fiscal Year 2008: ¥16,100,000 (Direct Cost: ¥16,100,000)
Fiscal Year 2007: ¥17,200,000 (Direct Cost: ¥17,200,000)
Fiscal Year 2006: ¥19,300,000 (Direct Cost: ¥19,300,000)
Fiscal Year 2005: ¥19,700,000 (Direct Cost: ¥19,700,000)
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| Keywords | リポソーム / 人工細胞モデル / その場観察 / DNA折り畳み転移 / 自己組織化 / ナノ構造制御 / 油中水滴 / レーザーピンセット / ミクロ液的 / ミクロ液滴 |
|---|
| Research Abstract |
The main purpose of this project is the construction of "non-equilibrium dissipative"artificial cell models. To achieve the purpose, the following three axes are employed; (i) in vitro re-construction of chromosome, (ii) transcriptional regulation by DNA coil-globule transition, (iii) cell-sized space specificity in the dynamics of membrane, DNA, and protein. The representative results in relation to the cell engineering are as follows; (i) Establishment of the methodology to entrap bio-polymers and substrates in a cell-sized model system. (ii) Unraveling of the scenario on the higher-order structural transition of chromatin. (iii) Invention of the technique of noninvasive and noncontact manipulation of bio-macromolecules by laser. Main results on basic science are; (i) Clear experimental representation on the confined effect of cell-sized space was demonstrated. For example, actin exhibits the two-step transition in a confined space, whereas it shows single step transduction in bulk phase. (ii) Clear evidence was shown on the cross-talk between membrane fluidity and the bulk viscosity.
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Report
(6 results)
Research Products
(90 results)
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[Presentation] Cationic Silica Nanoparticles are Efficiently Transferred into Mammalian Cells2007
Author(s)
L. Liu, S. Inagaki, K. Yoshikawa, Kyoto University, Japan, T. Takenaka, A. A. Zinchenko, N. Chen, H. Asada, T. Kishida, O. Mazda and S. Murata
Organizer
MHS2007 & Micro-Nano COE
Place of Presentation
名古屋大学野依記念学術交流間
Year and Date
2007-11-12
Related Report
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[Book] Methods in Enzymology2009
Author(s)
K. Takiguchi, A. Yamada, M. Negishi, M. Honda, Y. Tanaka-Takiguchi, K. Yoshikawa
Publisher
Elsevier Inc.
Related Report
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