|Budget Amount *help
¥76,100,000 (Direct Cost: ¥76,100,000)
Fiscal Year 2009: ¥10,000,000 (Direct Cost: ¥10,000,000)
Fiscal Year 2008: ¥10,000,000 (Direct Cost: ¥10,000,000)
Fiscal Year 2007: ¥17,700,000 (Direct Cost: ¥17,700,000)
Fiscal Year 2006: ¥19,200,000 (Direct Cost: ¥19,200,000)
Fiscal Year 2005: ¥19,200,000 (Direct Cost: ¥19,200,000)
In this project, we have obtained the following results;
i) By combining in vitro nucleosome reconstitution procedure and atomic force microscopy, "30-nm-chromatin fiber" was successfully reconstituted.
ii) By utilizing fast-scanning atomic force microscopy, molecular mechanisms of various enzymatic functions and processes have been elucidated.
iii) We established the technique to microinject a fluorescent particle into a living cell and manipulate it by optical trap without damaging the cell.
iv) By attaching nuclear localization signal peptide to quantum dots, we established the method to deliver nano-particles into a living cell nucleus.
v) Structural and functional properties of nuclear scaffold proteins have been obtained by combining proteomics, bioinformatics, molecular biology and biochemistry.