Molecular mechanism of uptake and translocation of essential metal nutrients in plants.
| Project/Area Number |
17078002
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research on Priority Areas
|
| Allocation Type | Single-year Grants |
|---|
| Review Section |
Biological Sciences
|
|---|
| Research Institution | The University of Tokyo |
|---|
Principal Investigator |
NISHIZAWA Naoko The University of Tokyo, 生物資源環境学部, 教授 (70156066)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
TAKAHASHI Michiko 宇都宮大学, 農学部, 准教授 (90345182)
|
|---|
| Project Period (FY) |
2005 – 2009
|
| Project Status |
Completed (Fiscal Year 2009)
|
| Budget Amount *help |
¥115,500,000 (Direct Cost: ¥115,500,000)
Fiscal Year 2009: ¥22,500,000 (Direct Cost: ¥22,500,000)
Fiscal Year 2008: ¥22,500,000 (Direct Cost: ¥22,500,000)
Fiscal Year 2007: ¥23,500,000 (Direct Cost: ¥23,500,000)
Fiscal Year 2006: ¥23,500,000 (Direct Cost: ¥23,500,000)
Fiscal Year 2005: ¥23,500,000 (Direct Cost: ¥23,500,000)
|
|---|
| Keywords | ミネラル栄養 / 金属錯体トランスポーター / ニコチアナミン / ムギネ酸 / 環境耐性作物 |
|---|
| Research Abstract |
Iron is essential for most living organisms and is required for normal plant growth. We identified two novel cis-acting elements, IDE1 and IDE2, in the promoter of iron-deficiency-inducible IDS2 gene and two rice transcription factors, IDEF1 (IDE-binding factor 1) and IDEF2, which specifically bind to IDE1 and IDE2, respectively. IDEF1 efficiently binds to the CATGC sequence within IDE1. IDEF1 transcripts are constitutively present in rice roots and leaves. Transgenic rice plants expressing IDEF1 tolerate iron deficiency in calcareous soil. Expression analysis of these transgenic plants revealed that IDEF1 positively regulates iron-deficiency-induced genes, including the ferrous iron transporter gene OsIRT1 and the iron-deficiency-induced transcription factor gene OsIRO2. OsIRO2 regulates the expression of various Fe-deficiency-inducible genes including those for phytosiderophore biosynthesis. IDEF2 recognizes CA[A/C]G[T/C][T/C/A][T/C/A] within IDE2. IDEF2 transcripts are constitutively present in rice roots and leaves. Microarray analysis showed that IDEF2 is involved in the regulation of Fe-deficiency-inducible genes, including the Fe(II)-nicotianamine transporter gene OsYSL2. These data suggest the presence of a sequential gene regulatory network that functions via novel cis element/trans factor interactions to promote the iron-deficiency response.
|
Report
(5 results)
Research Products
(119 results)
-
-
-
-
-
-
-
-
-
[Journal Article] Iron fortification of rice seeds through activation of the nicotianamine synthase gene.2009
Author(s)
Lee S, Jeon US, Lee SJ, Kim Y-K, Persson DP, Husted S, Schjorring JK, Kakei Y, Masuda H, Nishizawa NK, An G.
-
Journal Title
Proceedings of the National Academy of the USA 106
Pages: 22014-22019
Related Report
Peer Reviewed
-
-
-
[Journal Article] Rice metal-nicotianamine transporter, OsYSL2, is required for the long-distance transport of iron and manganese.2009
Author(s)
Ishimaru Y, Masuda H, Bashir K, Inoue H, Tsukamoto K, Takahashi M, Nakanishi H, Aoki N, Hirose T, Ohsugi R, Nishizawa NK.
-
Journal Title
The Plant Journal 62
Pages: 379-390
Related Report
Peer Reviewed
-
-
-
-
-
-
[Journal Article] A Novel NAC Transcription Factor, IDEF2, That Recognizes the Iron Deficiency-responsive Element 2 Regulates the Genes Involved in Iron Homeostasis in Plants.2008
Author(s)
Ogo Y, Kobayashi T, Nakanishi Itai R, Nakanishi H, Kakei Y, Takahashi M, Toki S, Mori S, Nishizawa NK.
-
Journal Title
Journal of Biological Chemistry 283
Pages: 13407-13417
NAID
Related Report
Peer Reviewed
-
-
-
-
-
-
-
-
-
[Journal Article] Mutational reconstructed ferric chelate reductase confers enhanced tolerance in rice to iron deficiency in calcareous soil.2007
Author(s)
Ishimaru Y, Kim S, Tsukamoto T, Oki H, Kobayashi T, Watanabe S, Matsuhashi S, Takahashi M, Nakanishi H, Mori S, Nishizawa NK.
-
Journal Title
Proceedings of the National academy of Science of the USA 104
Pages: 7373-7378
Related Report
Peer Reviewed
-
-
-
-
-
-
-
-
-
-
-
-
-
[Journal Article] Rice plants take up iron as an Fe^<3+>-phytosiderophore and as Fe^<2+>.2006
Author(s)
Ishimaru Y, Suzuki M, Tsukamoto T, Suzuki K, Nakazono M, Kobayashi T, Wada Y, Watanabe S, Matsuhashi S, Takahashi M, Nakanishi H, Mori S, Nishizawa NK.
-
Journal Title
The Plant Journal 45
Pages: 335-346
Related Report
Peer Reviewed
-
-
[Journal Article] Biosynthesis and secretion of mugineic acid family phytosiderophores in zinc-deficient barley.2006
Author(s)
Suzuki M, Takahashi M, Tsukamoto T, Watanabe S, Matsuhashi S, Yazaki J, Kishimoto N, Kikuchi S, Nakanishi H, Mori S, Nishizawa NK.
-
Journal Title
The Plant Journal 48
Pages: 85-97
Related Report
Peer Reviewed
-
[Journal Article] GAMYB controls different sets of genes and is differentially regulated by microRNA in aleurone cells and anthers.2006
Author(s)
Tsuji H, Aya K, Ueguchi-Tanaka M, Shimada Y, Nakazono M, Watanabe R, Nishizawa NK, Gomi K, Shimada A, Kitano H, Ashikari M, Matsuoka M.
-
Journal Title
The Plant Journal 47
Pages: 427-444
Related Report
Peer Reviewed
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
