Functional analysis of stress-responsive transporter genes in plants
| Project/Area Number |
17078003
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| Research Category |
Grant-in-Aid for Scientific Research on Priority Areas
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| Allocation Type | Single-year Grants |
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| Review Section |
Biological Sciences
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| Research Institution | The University of Tokyo |
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Principal Investigator |
SHINOZAKI Kazuko The University of Tokyo, 大学院・農学生命科学研究科, 教授 (30221295)
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| Co-Investigator(Kenkyū-buntansha) |
OSAKABE Yuriko 東京大学, 大学院・農学生命科学研究科, 講師 (50444071)
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| Project Period (FY) |
2005 – 2009
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| Project Status |
Completed (Fiscal Year 2009)
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| Budget Amount *help |
¥63,000,000 (Direct Cost: ¥63,000,000)
Fiscal Year 2009: ¥12,000,000 (Direct Cost: ¥12,000,000)
Fiscal Year 2008: ¥12,000,000 (Direct Cost: ¥12,000,000)
Fiscal Year 2007: ¥13,000,000 (Direct Cost: ¥13,000,000)
Fiscal Year 2006: ¥13,000,000 (Direct Cost: ¥13,000,000)
Fiscal Year 2005: ¥13,000,000 (Direct Cost: ¥13,000,000)
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| Keywords | 環境ストレス応答 / 浸透圧ストレス / 単糖トランスポーター / ジロイシン配列 / グルコース / ERD6 / ESL1 / 遺伝子発現制御 / マイクロアレイ / トランスポーター / シロイヌナズナ / 糖 / カリウム / ストレス / 発現制御 |
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| Research Abstract |
Plants respond to survive under water-deficit conditions via a series of physiological, cellular, and molecular processes culminating in stress tolerance. Plants accumulate osmolites, such as sugars, amino acids and potassium, during osmotic stress. We identified various transporter genes were upregulated during osmotic stress in Arabidopsis. We analyzed an osmotic stress-inducible sugar transporter, ESL1, from Arabidopsis. ESL1 is mainly expressed in pericycle and xylem parenchyma cells. The fluorescence of ESL1-GFP-fused protein was detected at tonoplast in transgenic plants. Alanine-scanning mutagenesis revealed that an N-terminal LXXXLL motif in ESL1 was essential for its localization at the tonoplast. Transgenic tobacco BY-2 cells expressing mutated ESL1, which was localized at the plasma membrane, showed the uptake ability for monosaccharides. The value of K(m) for glucose uptake activity of mutated ESL1 in the transgenic BY-2 cells was extraordinarily high, and the transport activity was independent from a proton gradient. These results indicate that ESL1 is a low affinity facilitated diffusion transporter. We also analyzed an Arabidopsis stress-inducible potassium transporter, KUP6. The localization of KUP6-GFP was observed at plasma membrane. KUP6-overexpressing transgenic plants showed less transpirational water loss and increased tolerance to drought stress. Furthermore, we also analyzed Arabidopsis stress-inducible transporter-like proteins, Cor413 family, whose expression was regulated by DREB1A during cold stress. COR413-IM1 and COR413-IM2.1 were localized at the chloroplast membrane, while COR413-PM1 was localized at ER. Furthermore, we used both gain- and loss-of-function analysis of a histidine kinase, AHK1, and showed that it acts as a positive regulator in osmotic stress signaling in Arabidopsis. Overexpression of AHK1 improved the drought tolerance of transgenic plants.
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Report
(6 results)
Research Products
(51 results)
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[Journal Article] Overproduction of the membrane-bound receptor-like protein kinase1, RPK1, enhances abiotic stress tolerance in Arabidopsis2010
Author(s)
Osakabe, Y., Mizuno, S., Tanaka, H., Maruyama, K., Osakabe, K., Todaka, D., Fujita, Y., Kobayashi, M., Shinozaki, K., Yamaguchi-Shinozaki, K.
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Journal Title
J. Biol. Chem. 285(12)
Pages: 9190-9201
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[Journal Article] Functional analysis of an Arabidopsis thaliana abiotic stress-inducible facilitated diffusion transporter for monosaccharides.2010
Author(s)
Yamada, K., Osakabe, Y., Mizoi, J., Nakashima, K., Fujita, Y., Shinozaki, K., Yamaguchi-Shinozaki, K.
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Journal Title
J. Biol. Chem. 285(2)
Pages: 1138-1146
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[Journal Article] Functional analysis of AHK1/ATHK1 and cytokinin receptor histidine kinases in response to abscisic acid, drought and salt stresses in Arabidopsis2007
Author(s)
Tran, L. -S.P., Urao, T., Qin, F., Maruyama, K., Kakimoto, T., Shinozaki, K.,, Yamaguci-Shinozaki, K.
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Journal Title
Proc. Natl. Acad. Sci. USA. 104
Pages: 20623-20628
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[Presentation] Functional analysis of an osmotic stress-inducible potassium transporter, KUP6, in Arabidopsis.2007
Author(s)
Arinaga, N., Osakabe, Y., Yamada, K., Maruyama, K., Shinozaki, K., Yamaguchi-Shinozki, K.
Organizer
7th APSRC International Symposium: Abiotic Stress and Plant Growth and Development
Place of Presentation
Gwangju, Korea
Year and Date
2007-11-08
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[Presentation] Functional analysis of osmotic stress-inducible sugar transporter genes in Arabidopsis.2007
Author(s)
Yamada, K., Osakabe, Y., Maruyama, K., Fujita, Y., Mizoi, J., Shinozaki, K., Yamaguchi-Shinozaki, K.
Organizer
2007 International Symposium on Plant Stress and Metabolism & Annual Meeting of the KSABC
Place of Presentation
Gyeongju, Korea
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