Study on plant Na and K transport system, which is involved in the formation of membrane potential and adaptation of osmolality

• Project/Area Number: 17078005
• Research Category: Grant-in-Aid for Scientific Research on Priority Areas
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: Tohoku University (2006-2009); Nagoya University (2005)
• Principal Investigator: UOZUMI Nobuyuki Tohoku University, 大学院・工学研究科, 教授 (40223515)
• Project Period (FY): 2005 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥83,500,000 (Direct Cost: ¥83,500,000); Fiscal Year 2009: ¥16,100,000 (Direct Cost: ¥16,100,000); Fiscal Year 2008: ¥16,100,000 (Direct Cost: ¥16,100,000); Fiscal Year 2007: ¥17,100,000 (Direct Cost: ¥17,100,000); Fiscal Year 2006: ¥17,100,000 (Direct Cost: ¥17,100,000); Fiscal Year 2005: ¥17,100,000 (Direct Cost: ¥17,100,000)
• Keywords: 浸透圧 / イオンチャネル / 塩 / ナトリウム / カリウム / 膜輸送 / 膜電位 / KAT1 / リン酸化 / ソルビトール / 水チャネル / 液胞 / シロイヌナズナ / カルシウム / ラン藻 / AtHKT1 / 輸送体 / 塩ストレス / TaHKT1 / AtNHX1 / Ktr系 / 道管 / Ktr / 原形質膜

Research Abstract

The membrane transport system is closely involved in the formation of membrane potential and adaptation to the abiotic stress. We aimed the physiological role of the channels and the transporters which mediated Na and/or K permeation across the plasma membrane and thylakoid membrane. There are some difference in Na and K transport system between animal cells and plant cells. We have studied the structure and function of the Na/K transporters and K channels in plant cells and cyanobacteria which are an essential system for the controlling the solute flux across the membrane.

Research Products

• [Journal Article] Phytosiderophore efflux transporters are crucial for iron acquisition in graminaceous plants. (2011)
  • Author(s): Nozoye, T., Nagasaka, S., Kobayashi, T., Takahashi, M., Sato, Y., Sato, Y., Uozumi, N., Nakanishi, H., Nishizawa, NK.
  • Journal Title: J.Biol.Chem. 286 Pages: 5446-5454
  • Peer Reviewed
• [Journal Article] AtKUP/HAK/KT9, a K^+ transporter from Arabidopsis thaliana, mediates Cs^+ uptake in Escherichia coli. (2010)
  • Author(s): Kobayashi, D., Uozumi, N., Hisamatsu, S., Yamagami, M.
  • Journal Title: Biosci.Biotech.Biochem. 74 Pages: 203-205
  • NAID: 10027550686
  • Peer Reviewed
• [Journal Article] Modulation of the Arabidopsis KAT1 channel by an activator of protein kinase C in Xenopus laevis oocytes. (2010)
  • Author(s): Sato, A., Gambale, F., Dreyer, I., Uozumi, N.
  • Journal Title: FEBS J. 277 Pages: 2318-2328
  • Peer Reviewed
• [Journal Article] Trk/HKT-type K^+ transporter from Trypanosoma brucei. (2010)
  • Author(s): Mosimann, M., Goshima, S., Wenzler, Y., Luscher, A., Uozumi, N., Maser, P.
  • Journal Title: Eukaryotic Cell 9 Pages: 539-546
  • Peer Reviewed
• [Journal Article] A novel potassium channel in photosynthetic cyanobacteria. (2010)
  • Author(s): Zanetti, M., Teardo, E., Rocca, N. L., Zulkifli, L., Checchetto, V., Shijuku, T, Sato, Y., Giacometti, G. M., Uozumi, N., Bergantino, E., Szabo, I.
  • Journal Title: PLoS ONE 5
  • Peer Reviewed
• [Journal Article] Roles of tandem-pore K^+ channels in plants-a puzzle still to be solved. (2010)
  • Author(s): Voelker, C., Gomez-Porras, J. L., Becker, D., Hamamoto, S., Uozumi, N., Gambale, F., Mueller-Roeber, B., Czempinski, K., Dreyer, I.
  • Journal Title: Plant Biology 12 Pages: 56-66
  • Peer Reviewed
• [Journal Article] Cell-free protein N-myristoylation system utilizing wheat-germ extracts facilitates update of consensus motif for the modification of plant proteins. (2010)
  • Author(s): Yamauchi, S., Fusada, N., Hayashi, H., Utsumi, T., Uozumi, N., Tozawa, Y.
  • Journal Title: FEBS.J. 277 Pages: 3596-3607
  • Peer Reviewed
• [Journal Article] The KtrA and KtrE subunits are required for Na^+ dependent K^+ uptake by KtrB across the plasma membrane in Synechocystis sp.PCC 6803. (2010)
  • Author(s): Zulkifli, L., Akai, M., Yoshikawa, A.,Shimojima, M., Ohta, H., Guy HR., Uozumi, N.
  • Journal Title: J.Bacteriol. 192 Pages: 5063-5070
  • Peer Reviewed
• [Journal Article] AtKUP/HAK/KT9, a K^+ transporter from Arabidopsis thaliana, mediates Cs^+ uptake in Escherichia coli. (2010)
  • Author(s): Kobayashi, et al.
  • Journal Title: Biosci.Biotech.Biochem. Volume: 74 Pages: 203-205
  • Peer Reviewed
• [Journal Article] Modulation of the Arabidopsis KAT1 channel by an activator of protein kinase C in Xenopus laevis oocytes. (2010)
  • Author(s): Sato, et al.
  • Journal Title: FEBS.J. Volume: 277 Pages: 2318-2328
  • Peer Reviewed
• [Journal Article] A novel potassium channel in photosynthetic cyanobacteria. (2010)
  • Author(s): Zanetti, et al.
  • Journal Title: PLoS ONE Volume: 5
  • Peer Reviewed
• [Journal Article] Roles of tandem-pore K^+ channels in plants-a puzzle still to be solved. (2010)
  • Author(s): Voelker, et al.
  • Journal Title: Plant Biology Volume: 12 Pages: 56-63
  • Peer Reviewed
• [Journal Article] The KtrA and KtrE subunits are required for Na^+ dependent K^+ uptake by KtrB across the plasma membrane in Synechocystis sp.PCC 6803. (2010)
  • Author(s): Zulkifli, et al.
  • Journal Title: J.Bacteriol. Volume: 192 Pages: 5063-5070
  • Peer Reviewed
• [Journal Article] Phytosiderophore efflux transporters are crucial for iron acquisition in graminaceous plants. (2010)
  • Author(s): Nozoye, et al.
  • Journal Title: J.Biol.Chem. Volume: 286 Pages: 5446-5454
  • Peer Reviewed
• [Journal Article] Contribution of salicylic acid glucosyltransferase, OsSGT1, to chemically-induced disease resistance in rice plants. (2009)
  • Author(s): Umemura, K., Satoh, J., Iwata, M., Uozumi, N., Koga, J., Kawano, T., Koshiba, T., Anzai, H., Mitomi, M.
  • Journal Title: Plant J. 57 Pages: 463-472
  • Peer Reviewed
• [Journal Article] Identification and characterization of the Na^+/H^+ antiporter NhaS3 from the thylakoid membrane of Synechocystis sp.PCC 6803. (2009)
  • Author(s): Tsunekawa, K., Shijuku, T., Hayashimoto, M., Kojima, T., Onai, K., Morishita, M., Ishiura, M., Kuroda, T., Nakamura, T., Kobayashi, H., Sato, M., Toyooka, K., Matsuoka, K., Omata, T., Uozumi, N.
  • Journal Title: J.Biol.Chem. 284 Pages: 16513-16521
  • Peer Reviewed
• [Journal Article] Synchrony between flower opening and petal-color change from red to blue in morning glory, Ipomoea tricolor cv. Heavenly Blue. (2009)
  • Author(s): Yoshida, K., Miki, N., Momonoi, K., Kawachi, M., Katou, K., Okazaki, Y., Uozumi, N., Maeshima, M., Kondo, T.
  • Journal Title: Proc.Jpn.Acad.Ser.B 85 Pages: 187-197
  • NAID: 130000138066
  • Peer Reviewed
• [Journal Article] Threonine at position 306 of the KAT1 potassium channel is essential for channel activity and is a target site for ABA-activated SnRK2/OST1/SnRK2.6 protein kinase. (2009)
  • Author(s): Sato, A., Sato, Y., Fukao, Y., Fujiwara, M., Umezawa, T., Shinozaki, K., Hibi, T., Taniguchi, M., Miyake, H., Goto, D.B., Uozumi, N.
  • Journal Title: Biochem.J. 424 Pages: 439-448
  • Peer Reviewed
• [Journal Article] The implication of YggT of Escherichia coli in osmotic regulation. (2009)
  • Author(s): Ito, T., Uozumi, N., Nakamura, T., Takayama, S., Matsuda, N., Aiba, H., Hemmi, H., Yoshimura, T.
  • Journal Title: Biosci.Biotech.Biochem. 73 Pages: 2698-2704
  • NAID: 10027549163
  • Peer Reviewed
• [Journal Article] Identification and characterization of the Na^+/H^+ antiporter NhaS3 from the thylakoid membrane of Synechocystis sp.PCC 6803 (2009)
  • Author(s): Tsunekawa, et al.
  • Journal Title: J.Biol.Chem. Volume: 284 Pages: 16513-16521
  • Peer Reviewed
• [Journal Article] Threonine at position 306 of the KAT1 potassium channel is essential for channel activity and is a target site for ABA-activated SnRK2/OST1/SnRK2.6 protein kinase (2009)
  • Author(s): Sato, et al.
  • Journal Title: Biochem.J. Volume: 424 Pages: 439-448
  • Peer Reviewed
• [Journal Article] Contribution of salicylic acid glucosyltransferase, OsSGT1, to chemically-induceddisease resistance in rice plants (2009)
  • Author(s): Umemura, K, et.al.
  • Journal Title: Plant J 57 Pages: 463-472
  • Peer Reviewed
• [Journal Article] Characterization of a tobacco TPK-type K^+ channel as a novel tonoplast K^+ channel using yeast tonoplasts. (2008)
  • Author(s): Hamamoto, S., Marui, K., Matsuoka, K., Higashi, K., Igarashi, K., Nakagawa, T., Kuroda, T., Mori, Y., Murata, Y., Nakanishi, Y., Maeshima, M., Yabe, I., Uozumi, N.
  • Journal Title: J.Biol.Chem. 283 Pages: 1911-1920
  • Peer Reviewed
• [Journal Article] Purification of the functional plant membrane channel KAT1. (2008)
  • Author(s): Hibi, T., Aoki; S., Oda; K., Munemasa, S., Ozaki, S., Shirai, O., Uozumi, N.
  • Journal Title: Biochem.Biophys.Res.Commun. 374 Pages: 465-469
  • Peer Reviewed
• [Journal Article] Novel treatment for lithium induced nephrogenic diabetes insipidus rat model using the Sendai-virus vector carrying aquaporin 2 gene. (2008)
  • Author(s): Suga, H., Nagasaki, H., Kondo, T., Okajima, Y., Suzuki, C., Ozaki, N., Arima, H., Yamamoto, T., Ozaki, N., Akai, M., Sato, A., Uozumi, N., Inoue, M., Hasegawa, M., Oiso, Y.
  • Journal Title: Endocrinology 149 Pages: 5803-5810
  • Peer Reviewed
• [Journal Article] Electrophysiological property of NtTPK1 expressed in yeast tonoplast. (2008)
  • Author(s): Hamamoto, S., Yabe, I., Uozumi, N.
  • Journal Title: Biosci.Biotech.Biochem. 72 Pages: 2785-2787
  • NAID: 10027533536
  • Peer Reviewed
• [Journal Article] Purification of the functional plant membrane channel KAT1 (2008)
  • Author(s): Hibi, T., et.al.
  • Journal Title: Biochem. Biophys. Res. Commun 374 Pages: 465-469
  • Peer Reviewed
• [Journal Article] Electrophysiological property of NtTPK1 expressed in yeast tonoplast (2008)
  • Author(s): Hamamoto, S, et.al.
  • Journal Title: Biosci. Biotech. Biochem 72 Pages: 2785-2787
  • Peer Reviewed
• [Journal Article] Novel treatment for lithium induced nephrogenic diabetes insipidus rat model using the Sendai-virus vector carrying aquaporin 2 gene (2008)
  • Author(s): Suga, H, et.al.
  • Journal Title: Endocrinology 149 Pages: 5803-5810
  • Peer Reviewed
• [Journal Article] Characterization of a tobacco TPK-type K^+ channel as a novel tonoplast K^+ channel using yeast tonoplasts (2008)
  • Author(s): Hamamoto et al.
  • Journal Title: J. Biol. Chem. 283 Pages: 1911-1920
  • Peer Reviewed
• [Journal Article] Contribution of hydrophobic and electrostatic interactions to the membrane integration of the Shaker K^+ channel voltage sensor domain. (2007)
  • Author(s): Zhang, L., Sato, Y., Hessa, T., von Heijne, G., Lee, J-K., Kodama, I., Sakaguchi, M., Uozumi, N.
  • Journal Title: Proc.Natl.Acad.Sci.USA 104 Pages: 8263-8268
  • Peer Reviewed
• [Journal Article] Role of positively charged amino acids in the M2_D transmembrane helix of Ktr/Trk/HKT-type cation transporters. (2007)
  • Author(s): Kato, N., Akai, M., Zulkifli, L., Matsuda, N., Kato, Y., Goshima, S., Hazama, A., Yamagami, M., Guy, H. R., Uozumi, N.
  • Journal Title: Channels 1 Pages: 161-171
  • Peer Reviewed
• [Journal Article] Contribution of hydrophobic and electrostatic interactions to the membrane integration of the Shaker K^+ channel voltage sensor domain (2007)
  • Author(s): Zhang et al.
  • Journal Title: Proc. Natl. Acad. Sci. USA 104 Pages: 8263-8268
  • Peer Reviewed
• [Journal Article] Role of positively charged amino acids in the M2_D transmembrane helix of Ktr/Trk/HKT-type cation transporters (2007)
  • Author(s): Kato et al.
  • Journal Title: Channels 1 Pages: 161-171
  • Peer Reviewed
• [Journal Article] Ktr-mediated potassium transport, a major pathway for potassium uptake, is coupled to a proton gradient across the membrane in the Synechocystis sp.PCC6803. (2006)
  • Author(s): Matsuda, N., Uozumi, N.
  • Journal Title: Biosci.Biotech.Biochem. 70 Pages: 273-275
  • Peer Reviewed
• [Journal Article] Properties of Shaker-type potassium channels in higher plants. (2006)
  • Author(s): Gambale, F., Uozumi, N.
  • Journal Title: J.Membrane Biol. 210 Pages: 1-19
  • Peer Reviewed
• [Journal Article] Nomenclature for HKT transporters, key determinants of plant salinity tolerance. (2006)
  • Author(s): Platten, J.D., Cotsaftis, O., Berthomieu, P., Bohnert, H., Davenport, R.J., Fairbairn, D.J., Horie, T., Leigh, R.A., Lin, H-X., Luan, S., Maser, P., Pantoja, O., Rodriguez-Navarro, A., Schachtman, D.P., Schroeder, J.I., Sentenac, H., Uozumi, N., Very, A-A., Zhu, J-K., Dennis, E.S., Tester, M.
  • Journal Title: Trends in Plant Sciences 11 Pages: 372-374
  • Peer Reviewed
• [Journal Article] Mutation of His-157 in the second pore loop drastically reduces the activity of Synechocystis Ktr transporter. (2006)
  • Author(s): Zulkifli, L., Uozumi, N.
  • Journal Title: J.Bacteriol. 188 Pages: 7985-7987
  • Peer Reviewed
• [Journal Article] Properties of Shaker-type potassium channels in higher plants. (2006)
  • Author(s): Gambale, F.
  • Journal Title: J. Membrane Biol. 210 Pages: 1-19
• [Journal Article] Nomenclature for HKT transporters, key determinants of plant salinity tolerance (2006)
  • Author(s): Platten, J.D., et al.
  • Journal Title: Trends in Plant Sciences 11 Pages: 372-374
• [Journal Article] Mutation of His-157 in the second pore loop drastically reduces the activity of Synechocystis Ktr transporter. (2006)
  • Author(s): Zulkifli, L.
  • Journal Title: J. Bacteriol. 188 Pages: 7985-7987
• [Journal Article] チャネルタンパク質の膜への組込み様式 (2006)
  • Author(s): 魚住信之
  • Journal Title: イオンチャネル・受容体の構造機能連関研究の新たな潮流 25・3 Pages: 264-268
• [Journal Article] Ktr-mediated potassium transport, a major pathway for potassium uptake, is coupled to a proton gradient across the membrane in the Synechocystis sp. PCC6803 (2006)
  • Author(s): Matsuda et al.
  • Journal Title: Biosci.Biotech.Biochem. 70 Pages: 273-275
• [Journal Article] Further application of a two-step heparin affinity chromatography method using divalent cations as eluents : Purification and identification of membrane-bound heparinb inding proteins from the mitochondrial fraction of HL-60 cells. (2005)
  • Author(s): Iida, T., Masaharu, K., Uozumi, N., Inui, T., Imai, K.
  • Journal Title: J.Chromatography B 823 Pages: 209-212
  • NAID: 10016980232
  • Peer Reviewed
• [Journal Article] Enhanced salt tolerance mediated by AtHKT1 transporter-induced Na^+ unloading from xylem vessels to xylem parenchyma cells. (2005)
  • Author(s): Sunarpi, Horie, T., Motoda, Jo, Kubo, M., Yang, H., Yoda, K., Horie, R., Chan, W-Y., Leung, H-Y., Hattori, K., Konomi, M., Osumi, M., Yamagami, M., Schroeder, J.I.,U ozumi, N.
  • Journal Title: Plant J. 44 Pages: 928-938
  • Peer Reviewed
• [Journal Article] All four putative selectivity filter glycine residues in KtrB are essential for high affinity and selective K^+ uptake by the KtrAB system from Vibrio alginolyticus. (2005)
  • Author(s): Tholema, N., vor der Bruggen, M., Maser, P., Nakamura, T., Schroeder, J.I., Kobayashi, H., Uozumi, N., Bakker, E.P.
  • Journal Title: J.Biol.Chem. 280 Pages: 41146-41154
  • Peer Reviewed
• [Journal Article] Further application of a two-step heparin affinity chromatography method using divalent cations as eluents : Purification and identification of membrane-bound heparin binding proteins from the mitochondrial fraction of HL-60 cells. (2005)
  • Author(s): Iida et al.
  • Journal Title: J.Chromatography B 823 Pages: 209-212
• [Journal Article] Enhanced salt tolerance mediated by AtHKT1 transporter-induced Na^+ unloading from xylem vessels to xylem parenchyma cells (2005)
  • Author(s): Sunarpi et al.
  • Journal Title: Plant J 44 Pages: 928-938
• [Journal Article] All four putative selectivity filter glycine residues in KtrB are essential for high affinity and selective K^+ uptake by the KtrAB system from Vibrio alginolyticus. (2005)
  • Author(s): Tholema et al.
  • Journal Title: J.Biol.Chem. 280 Pages: 41146-41154
• [Presentation] KAT1 is differentially phosphorylated by SnRK2.6 and calcium-dependent kinase, which reduces its transport activity. (2010)
  • Author(s): Uozumi N, Sato A, Sato Y, Fukao Y, Fujiwara M, Umezawa T, Shinozaki K, Hibi T, Taniguchi M, Miyake M, Dreyer I, Gambale F, Goto D.
  • Organizer: XV International Workshop on Plant Membrane Biology
  • Place of Presentation: Adelaide, Australia
  • Year and Date: 2010-09-23
• [Presentation] KAT1のリン酸化によるK+輸送活性調節 (2009)
  • Author(s): 佐藤愛子他
  • Organizer: 日本農芸化学会2009年度大会
  • Place of Presentation: 福岡
  • Year and Date: 2009-03-28
• [Presentation] マイクロ流路を用いたラン藻の高浸透圧ストレスに対する適応機構のリアルタイム観察 (2009)
  • Author(s): 四十九俊彰他
  • Organizer: 日本植物生理学会年会
  • Place of Presentation: 名古屋
  • Year and Date: 2009-03-21
• [Presentation] Phosphorylation Of KAT1 C-terminus Modulates K+ Uptake Activity (2009)
  • Author(s): Sato, A., Taniguchi, M., Miyake, H., Umezawa, T., Shinozaki, K., Goto, D.B., Uozumi, N.
  • Organizer: Biophysical Society 53rd Annual Meeting
  • Place of Presentation: Boston, USA
  • Year and Date: 2009-03-02
• [Presentation] タバコ液胞型Kチャネルの酵母を用いたパッチクランプ解析 (2008)
  • Author(s): 浜本晋他
  • Organizer: 日本生体エネルギー研究会
  • Place of Presentation: 東京
  • Year and Date: 2008-11-07
• [Presentation] 大腸菌と酵母を宿主として利用する植物イオンチャネル・トランスポーターの解析法 (2008)
  • Author(s): 魚住信之
  • Organizer: 日本生物工学会
  • Place of Presentation: 仙台
  • Year and Date: 2008-08-28
• [Presentation] マイクロ流路を用いたラン藻の浸透圧適応のリアルタイム観察 (2008)
  • Author(s): 四十九俊彰他
  • Organizer: 日本生物工学会
  • Place of Presentation: 仙台
  • Year and Date: 2008-08-28
• [Presentation] Membrane integration of the voltage sensor of the Shaker channel (2008)
  • Author(s): 魚住信之
  • Organizer: 52th Biophysical Meeting
  • Place of Presentation: Los Angles USA
  • Year and Date: 2008-02-04
• [Presentation] Shakerチャネルのトポロジー形成における疎水的および静電相互作用の役割 (2007)
  • Author(s): 佐藤陽子
  • Organizer: 第80回日本生化学会大会合同大会
  • Place of Presentation: 横浜
  • Year and Date: 2007-12-13
• [Presentation] Structure and function of K channel and Na/K transporter (2007)
  • Author(s): 魚住信之
  • Organizer: The 2nd Membrane Protein Conference
  • Place of Presentation: Dae jeon Korea
  • Year and Date: 2007-11-29
• [Presentation] Functional expression of tobacco K^+ channels in yeast tonoplast. (2007)
  • Author(s): 浜本 他
  • Organizer: 14th international workshop of the plant Membrane Biology
  • Place of Presentation: Valencia, Spain
  • Year and Date: 2007-06-27
• [Presentation] Contribution of hydrophobic and electrostatic interactions to the membrane integration of the K channel voltage sensor domain (2007)
  • Author(s): 魚住 他
  • Organizer: 14th international workshop of the Plant Membrane Biology
  • Place of Presentation: Valencia, Spain
  • Year and Date: 2007-06-27
• [Presentation] Membrane topogenesis of the voltage dependent K channels (2007)
  • Author(s): Zhang, L., Uozumi, N.
  • Organizer: Membrane Transport as a Universal Biological Mechanism
  • Place of Presentation: Kyoto
  • Year and Date: 2007-01-11
• [Book] Ion Channels and Plant Stress Responses.(Ion channels and plant stress : past, present and future)(In Demidchik, V., Maathuis, F. (ed.)) (2010)
  • Author(s): Uozumi, N., Schroeder J.I.
  • Publisher: Springer-Verlag Berlin Heidelberg.
• [Book] チャネルタンパク質の膜への組込み様式(イオンチャネル・受容体の構造機能連関研究の新たな潮流 久保義弘監修)細胞工学 (2006)
  • Author(s): 魚住信之
  • Publisher: 秀潤社
• [Book] 膜電位依存性Kチャネル(イオンチャネル 最前線update 倉智嘉久監修)別冊・医学のあゆみ (2005)
  • Author(s): 魚住信之
  • Publisher: 医歯薬出版株式会社
• [Book] Comprehensive Biophysics Volume 6-Channel Proteins(Structure-Function Correlates in Plant Ion Channels.)(Montal, M.(ed.))
  • Author(s): Uozumi, N, Dreyer, I.
  • Publisher: Elsevier, in press
• [Remarks] ホームページ等