New research initiatives in the study of G-protein signaling systems integrating cell communication network

• Project/Area Number: 17079001
• Research Category: Grant-in-Aid for Scientific Research on Priority Areas
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: The University of Tokyo
• Principal Investigator: KATADA Toshiaki 東京大学, 大学院・薬学系研究科, 教授 (10088859)
• Co-Investigator(Kenkyū-buntansha): KANAHO Yasunori 筑波大学, 大学院・人間総合科学研究科, 教授 (00214437) ; NEGISHI Manabu 京都大学, 生命科学研究科, 教授 (60201696) ; ITOH Hiroshi 奈良先端科学技術大学院大学, バイオサイエンス研究科, 教授 (10183005)
• Project Period (FY): 2005 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥41,900,000 (Direct Cost: ¥41,900,000); Fiscal Year 2010: ¥3,000,000 (Direct Cost: ¥3,000,000); Fiscal Year 2009: ¥9,300,000 (Direct Cost: ¥9,300,000); Fiscal Year 2008: ¥7,400,000 (Direct Cost: ¥7,400,000); Fiscal Year 2007: ¥9,300,000 (Direct Cost: ¥9,300,000); Fiscal Year 2006: ¥7,400,000 (Direct Cost: ¥7,400,000); Fiscal Year 2005: ¥5,500,000 (Direct Cost: ¥5,500,000)
• Keywords: シグナル伝達 / G蛋白質 / Gサイクル / ゲノムプロジェクト / 遺伝子 / タンパク質 / 蛋白質

Research Abstract

本特定領域「G蛋白質シグナル」研究は、これまでに多様な専門性と幅広い手法から主に動物細胞を対象にG蛋白質シグナルの研究を精力的に進めてきた8つの研究者グループ(研究代表者:堅田、根岸、渡邊、倉智、伊東、黒瀬、金保、望月)で「計画研究」を組織して、平成17年度後半から実質的な研究を開始した。翌平成18年度からは、一人もしくは少人数の研究者による32の「公募研究」も加わり、さらに平成20年度には、8つの「計画研究」の継続に加えて、26の「公募研究」が新たに再編され、平成21年度をもって実質的な研究の設定期間を終了した。これを受けて平成22年度は、総括班員(堅田、金保、根岸、伊東)が中心になって研究成果の取纏め・公表に向けて以下の活動を進めた。
1.事後評価ヒアリングに向けて、組織されたすべての班員に研究成果の取纏めを依頼し、総括班会議の開催等を通じて本特定領域「G蛋白質シグナル」研究で得られた成果等について取り纏めを進めた。2.本特定領域研究で得られた研究成果を、各種の学術集会において発表すると共に、学術雑誌に公表した。3.本特定領域ホームページやニューズレター等を通じて、本特定領域研究で得られた研究成果を国の内外に広く公表・発信した。4.本特定領域研究で得られた研究成果を、指定様式の「研究成果報告書」冊子体(A4判846頁、CD版)として作成し、各班員・外部評価委員、その他関係各位に配布した。

Research Products

• [Journal Article] RhoA activation participates in rearrangement of processing bodies and release of nucleated AU-rich mRNAs. (2011)
  • Author(s): Takahashi S, Sakurai K, Ebihara A, Kajiho H, Saito K, Kontani K, Nishina H, Katada T.
  • Journal Title: Nucleic Acids Res. (Epub ahead of print)
  • Peer Reviewed
• [Journal Article] The Arf-like GTPase Arl8 mediates delivery of endocytosed macromolecules to lysosomes in Caenorhabditis elegans. (2010)
  • Author(s): Nakae I, et al.
  • Journal Title: Mol.Biol.Cell Volume: 21 Pages: 2434-2442
  • Peer Reviewed
• [Journal Article] An Arf-like small G protein, ARL-8, promotes the axonal transport of presynaptic cargoes by suppressing vesicle aggregation. (2010)
  • Author(s): Klassen MP, et al.
  • Journal Title: Neuron Volume: 66 Pages: 710-723
  • Peer Reviewed
• [Journal Article] Joubert syndrome Arl13b functions at ciliary membranes and stabilizes protein transport in Caenorhabditis elegans. (2010)
  • Author(s): Cevik S, et al.
  • Journal Title: J.Cell Biol. Volume: 188 Pages: 953-969
  • Peer Reviewed
• [Journal Article] Gタンパク質研究の動向 (2010)
  • Author(s): 堅田利明
  • Journal Title: 細胞(The Cell) Volume: 42(3) Pages: 90-91
• [Journal Article] RASSF7 negatively regulates pro-apoptotic JNK signaling by inhibiting the activity of phosphorylated-MKK7. (2010)
  • Author(s): Takahashi S, Ebihara A, Kajiho H, Kontani K, Nishina H, Katada T.
  • Journal Title: Cell Death Differ. Nov19.(Epub ahead of print)
  • Peer Reviewed
• [Journal Article] The Arf-like GTPase Arl8 mediates delivery of endocytosed macromolecules to lysosomes in Caenorhabditis elegans. (2010)
  • Author(s): Nakae I, Fujino T, Kobayashi T, Sasaki A, Kikko Y, Fukuyama M, Gengyo-Ando K, Mitani S, Kontani K, Katada T.
  • Journal Title: Mol.Biol.Cell 21 Pages: 2434-2442
  • Peer Reviewed
• [Journal Article] Joubert syndrome Arl13b functions at ciliary membranes and stabilizes protein transport in Caenorhabditis elegans. (2010)
  • Author(s): Cevik S, Hori Y, Kaplan OI, Kida K, Toivenon T, Foley-Fisher C, Cottell D, Katada T, Kontani K, Blacque OE.
  • Journal Title: J.Cell Biol. 188 Pages: 953-969
  • Peer Reviewed
• [Journal Article] Pertussis toxin up-regulates angiotensin type 1 receptors through Toll-like receptor 4-mediated Rac activation. (2010)
  • Author(s): Nishida M, Suda R.Nagamatsu Y, Tanabe S, Onohara N, Nakaya M, Kanaho Y, Shibata T, Uchida K., Sumimoto H, Sato Y, Kurose H.
  • Journal Title: J.Biol.Chem. 285 Pages: 15268-15277
  • Peer Reviewed
• [Journal Article] Structural basis for the specific inhibition of heterotrimeric Gq protein by a small molecule. (2010)
  • Author(s): Nishimura A, Kitano K, J.Takasaki J, Taniguchi M, Mizuno N, Tago K, Hakoshima T, Itoh H.
  • Journal Title: Proc.Natl.Acad.Sci.USA 107 Pages: 13666-13671
  • Peer Reviewed
• [Journal Article] Ric-8B stabilizes the alpha subunit of stimulatory G protein by inhibiting its ubiquitination. (2010)
  • Author(s): Nagai Y, Nishimura A, Tago K, Mizuno N, Itoh H.
  • Journal Title: J.Biol.Chem. 285 Pages: 11114-11120
  • Peer Reviewed
• [Journal Article] Mechanism and role of high density lipoprotein-induced activation of AMP-activated protein kinase in endothelial cells. (2010)
  • Author(s): Kimura T, Tomura T, Sato K, Ito M, Matsuoka I, Im D, Kuwabara A, Mogi C, Itoh H, Kurose H, Murakami M, Okajima F.
  • Journal Title: J.Biol.Chem. 285 Pages: 4387-4397
  • Peer Reviewed
• [Journal Article] Gタンパク質研究の動向 (2010)
  • Author(s): 堅田利明
  • Journal Title: 細胞(TheCell) 42(3) Pages: 90-91
• [Journal Article] Arf-like protein 13B. (2009)
  • Author(s): Kontani K, Hori Y, Katada T.
  • Journal Title: Nature Molecule Pages (Published online)
  • Peer Reviewed
• [Journal Article] Phospholipase D signaling and its involvement in neurite outgrowth. (2009)
  • Author(s): Kanaho Y, Funakoshi Y, Hasegawa H.
  • Journal Title: Biochim.Biophys.Acta 1791 Pages: 898-904
  • Peer Reviewed
• [Journal Article] Sequential regulation of DOCK2 dynamics by two phospholipids during neutrophil chemotaxis. (2009)
  • Author(s): Nishikimi A, Fukuhara H, Su W, Hongu T, Takasuga S, Mihara H, Cao Q, Sanematsu F, Kanai M, Hasegawa H, Tanaka Y, Shibasaki M, Kanaho Y, Sasaki T, Frohman MA, Fukui Y.
  • Journal Title: Science 324 Pages: 384-387
  • Peer Reviewed
• [Journal Article] RhoG promotes neural progenitor cell proliferation in mouse cerebral cortex. (2009)
  • Author(s): Fujimoto S, Negishi M, Katoh H.
  • Journal Title: Mol.Biol.Cell 20 Pages: 4941-4950
  • Peer Reviewed
• [Journal Article] Plexin-B1 is a GTPase activating protein for M-Ras, remodeling dendrite morphology. (2009)
  • Author(s): Saito Y, Oinuma I, Fujimoto S, Negishi M.
  • Journal Title: EMBO Rep. 10 Pages: 614-621
  • Peer Reviewed
• [Journal Article] Different requirement of Rnd GTPases for R-Ras GAP activity of Plexin-C1 and Plexin-D1. (2009)
  • Author(s): Uesugi K, Oinuma I, Katoh H, Negishi M.
  • Journal Title: J.Biol.Chem. 284 Pages: 6743-6751
  • Peer Reviewed
• [Journal Article] Licochalcone A potently inhibits TNFa-induced NF-kB activation through the direct inhibition of IKK activation. (2009)
  • Author(s): Funakoshi-Tago M, Tanabe S, Tago K, Itoh H, Mashino T, Sonoda Y, Kasahara T.
  • Journal Title: Mol Pharmacol. 76 Pages: 745-753
  • Peer Reviewed
• [Journal Article] G-protein signalling negatively regulates the stability of aryl hydrocarbon receptor. (2009)
  • Author(s): Nakata A, Urano D, Fujii-Kuriyama Y, Mizuno N, Tago K, Itoh H.
  • Journal Title: EMBO Rep. 10 Pages: 622-628
  • Peer Reviewed
• [Journal Article] Biochemical characterization of missense mutations in the Arf/Arl-family small GTPase Arl6 causing Bardet-Biedl syndrome (2009)
  • Author(s): Kobayashi T., et al.
  • Journal Title: Biochem.Biophys.Res.Commun. 381 Pages: 439-442
  • Peer Reviewed
• [Journal Article] Arf-like protein 13B (2009)
  • Author(s): Kontani K, Hori Y, Katada T.
  • Journal Title: Nature Molecule Pages(Published online) doi:10.1038/mp.a003975.01
  • Peer Reviewed
• [Journal Article] Upf1 potentially serves as a RING-related E3 ubiquitin ligase via its association with Upf3 in yeast. (2008)
  • Author(s): Takahashi S, Araki Y, Ohya Y, Sakuno T, Hoshino S, Kontani K, Nishina H, Katada T.
  • Journal Title: RNA 14 Pages: 1950-1958
  • Peer Reviewed
• [Journal Article] RalA functions as an indispensable signal mediator for nutrient sensing system. (2008)
  • Author(s): Maehama T, Tanaka M, Nishina H, Murakami M, Kanaho Y, Hanada K.
  • Journal Title: J.Biol.Chem. 283 Pages: 35053-35059
  • Peer Reviewed
• [Journal Article] Loss of PIP5KIb demonstrates that PIP5KI isoform-specific PIP2 synthesis is required for IP3 formation. (2008)
  • Author(s): Wang Y, Chen X, Lian L, Tang T, Stalker TJ, Sasaki T, Kanaho Y, Brass LF, Choi JK, Hartwig JH, Abrams CS.
  • Journal Title: Proc.Natl.Acad.Sci.USA. 105 Pages: 14064-16069
  • Peer Reviewed
• [Journal Article] Orphan G-protein-coupled receptor GPR56 regulates neural progenitor cell migration via a Galpha 12/13 and Rho pathway. (2008)
  • Author(s): Iguchi T, Sakata K, Yoshizaki K, Tago K, Mizuno N, Itoh H.
  • Journal Title: J.Biol.Chem. 283 Pages: 14469-14478
  • Peer Reviewed
• [Journal Article] Tyr-phosphorylation signals translocate RIN3, the small GTPase Rab5-GEF, to early endocytic vesicles (2008)
  • Author(s): Yoshikawa M, et.al.
  • Journal Title: Biochem. Biophys. Res. Commun 372 Pages: 168-172
  • Peer Reviewed
• [Journal Article] Domain architecture of the atypical Arf-amily GTPase Arl13b involved in cilia formation (2008)
  • Author(s): Hori Y, et.al.
  • Journal Title: Biochem. Biophys. Res. Commun 373 Pages: 119-124
  • Peer Reviewed
• [Journal Article] Upf1 potentially serves as a RING-elated E3 ubiquitin ligase via its association with Upf3 in yeast (2008)
  • Author(s): Takahashi S, et.al.
  • Journal Title: RNA 14 Pages: 1950-1958
  • Peer Reviewed
• [Journal Article] Antagonistic control of cell fates by JNK and p38-MAPK Signaling. (2008)
  • Author(s): Wada T, et. al.
  • Journal Title: Cell Death and Differentiation 15 Pages: 89-93
  • Peer Reviewed
• [Journal Article] Mechanism of mRNA deadenylation : evidence for a molecular interplay between translation termination factor eRF3 and mRNA deadenylases. (2007)
  • Author(s): Funakoshi Y, Doi Y, Hosoda N, Uchida N, Osawa M, Shimada I, Tsujimoto M, Suzuki T, Katada T, Hoshino S.
  • Journal Title: Genes Dev. 23 Pages: 3135-3148
  • Peer Reviewed
• [Journal Article] Activation of the c-Jun N-terminal kinase pathway by MST1 is essential and sufficient for the induction of chromatin condensatindensation during apoptosis. (2007)
  • Author(s): Ura S, Nishina H, Gotoh Y, Katada T.
  • Journal Title: Mol Cell Biol. 27 Pages: 5514-5522
  • Peer Reviewed
• [Journal Article] Role of activation of PIP5Kg661 by AP-2 complex in synaptic vesicle endocytosis. (2007)
  • Author(s): Nakano-Kobayashi A, Yamazaki M, Unoki T, Hongu T, Murata C.Taguchi R, Katada T, Frohman MA, Yokozeki T, Kanaho Y.
  • Journal Title: EMBO J. 26 Pages: 1105-1116
  • Peer Reviewed
• [Journal Article] Control of cell polarity and motility by the PtdIns(3,4,5)P(3) phosphatase SHIP1. (2007)
  • Author(s): Nishio M, Watanabe K, Sasaki J, Taya C, Takasuga S, Iizuka R, Balla T, Yamazaki M, Watanabe H, Itoh R, Kuroda S, Horie Y, Forster I, Mak TS, Yonekawa H, Penninger JM, Kanaho Y, Suzuki A, Sasaki T.
  • Journal Title: Nat.Cell Biol. 9 Pages: 36-44
  • Peer Reviewed
• [Journal Article] Rac-GAP a-chimerin regulates motor-curcuit formation as a key mediator of Ephrin B3/EphA4 forward signaling. (2007)
  • Author(s): Iwasato T, Katoh H, Nishimaru H, Ishikawa Y, Inoue H, Saito Y, Ando R, Iwama M, Takahashi R, Negishi M, Itohara S.
  • Journal Title: Cell 130 Pages: 742-753
  • Peer Reviewed
• [Journal Article] Activation of the c-Jun N-terminal kinase pathway by MST1 is essential and sufficient for the induction of chromatin condensation during apotosis. (2007)
  • Author(s): Ura S, et. al.
  • Journal Title: Molecular and Cellular Biology 27 Pages: 5514-5522
  • Peer Reviewed
• [Journal Article] Cafl regulates translocation of ribonucleotide reductase by releasing nucleoplasmic Spo1-Suc22 assembly. (2007)
  • Author(s): Takahashi S, et. al.
  • Journal Title: Nucleic Acid Research 35 Pages: 1187-1197
  • Peer Reviewed
• [Journal Article] Caf1 regulates translocation of ribonucleotide reductase by releasing nucleoplasmic Spd1-Suc22 assembly. (2007)
  • Author(s): Takahashi S, et al.
  • Journal Title: Nucleic Acid Res. 35(In press)
• [Journal Article] Role of activation of PIP5Kγ661 by AP-2 complex in synaptic vesicle endocytosis. (2007)
  • Author(s): Nakano-Kobayashi, et al.
  • Journal Title: EMBO J. 26 Pages: 1105-1116
• [Journal Article] Ric-8A potentiates Gq-mediated signal transduction by acting downstream of G protein-coupled receptor in intact cells. (2007)
  • Author(s): Nishimura A, et al.
  • Journal Title: Genes Cells 12(In press)
• [Journal Article] Crucial role of the small GTPase ARF6 in hepatic cord formation during liver development. (2006)
  • Author(s): Suzuki T, Kanai Y, Hara T, Sasaki J, Sasaki T, Kohara M, Maehama T, Taya C, Shitara H, Yonekawa H, Frohaman MA, Yokozeki T, Kanaho Y.
  • Journal Title: Mol.Cell.Biol. 26 Pages: 6149-6156
  • Peer Reviewed
• [Journal Article] Regulation of neuronal morphology by Toca-1, an F-BAR/EFC protein that induces plasma membrane invagination. (2006)
  • Author(s): Kakimoto T, Katoh H, Negishi M.
  • Journal Title: J.Biol.Chem. 281 Pages: 29042-29053
  • Peer Reviewed
• [Journal Article] Sema4D/Plexin-B1 activates GSK-3b via R-Ras GAP activity, inducing growth cone collapse. (2006)
  • Author(s): Ito Y, Oinuma I, Katoh H, Kaibuchi K, Negishi M.
  • Journal Title: EMBO Rep. 7 Pages: 704-709
  • Peer Reviewed
• [Journal Article] Semaphorin 4D/Plexin-B1-mediated R-Ras GAP activity inhibits cell migration by regulating b1 integrin activity. (2006)
  • Author(s): Oinuma I, Katoh H, Negishi M.
  • Journal Title: J.Cell Biol. 173 Pages: 601-613
  • Peer Reviewed
• [Journal Article] Activation of Rac1 by RhoG regulates cell migration. (2006)
  • Author(s): Katoh H, Hiramoto K, Negishi M.
  • Journal Title: J.Cell Sci. 119 Pages: 56-65
  • Peer Reviewed
• [Journal Article] Ric-8A potentiates Gq-mediated signal transduction by acting downstream of G protein-coupled receptor in intact cells. (2006)
  • Author(s): Nishimura A, Okamoto M, Sugawara Y, Mizuno N, Yamauchi J, Itoh H.
  • Journal Title: Genes Cells 11 Pages: 487-498
  • Peer Reviewed
• [Journal Article] Release of RASSF1C from the nucleus by Daxx degradation links DNA damage and SAPK/JNK activation. (2006)
  • Author(s): Kitagawa D, et al.
  • Journal Title: EMBO J. 25 Pages: 3286-3297
• [Journal Article] Crucial role of the samll GTPase ARF6 in hepatic cord formation during liver development. (2006)
  • Author(s): Suzuki T, et al.
  • Journal Title: Mol. Cell. Biol. 26 Pages: 6149-6156
• [Journal Article] Semaphorin 4D/Plexin-B1-mediated R-Ras GAP activity inhibits cell migration by regulating β1 integrin activity. (2006)
  • Author(s): Oinuma I, et al.
  • Journal Title: J. Cell Biol. 173 Pages: 601-613
• [Journal Article] Activation of Rac1 by RhoG regulates cell migration. (2006)
  • Author(s): Katoh, et al.
  • Journal Title: J.Cell.Sci. 119 Pages: 56-65
• [Journal Article] G-protein- coupled receptor signaling through Gq and JNK negatively regulates neural progenitor cell migration. (2005)
  • Author(s): Mizuno N, Kokubu H, Sato M, Nishimura A, Yamauchi J, Kurose H, Itoh H.
  • Journal Title: Proc.Natl.Acad.Sci.USA 102 Pages: 12365-12370
  • Peer Reviewed
• [Journal Article] Novel role of the small GTPase Rheb : Its implication in endocytic pathway independent of the activation of mammalian target of rapamycin. (2005)
  • Author(s): Saito K, et al.
  • Journal Title: J.Biochem. 137 Pages: 423-430
• [Journal Article] Purification and analysis of RIN family - novel Rab5 GEFs. (2005)
  • Author(s): Saito K, et al.
  • Journal Title: Methods Enzymol. 403 Pages: 276-283
• [Journal Article] The small GTPase ADP-ribosylation factor 6 negatively regulates dendritic spine formation. (2005)
  • Author(s): Miyazaki H, et al.
  • Journal Title: FEBS Lett. 579 Pages: 6834-6837
• [Journal Article] Direct interaction of Rnd1 with FRS2b regulates Rnd1-induced down-regulation of RhoA activity and is involved in FGF-induced neurite outgrowth in PC12 cells. (2005)
  • Author(s): Harada, et al.
  • Journal Title: J.Biol.Chem. 280 Pages: 18418-18424
• [Journal Article] G protein-coupled receptor signaling through Gq and JNK negatively regulates neural progenitor cell migration. (2005)
  • Author(s): Mizuno N, et al.
  • Journal Title: Proc.Natl.Acad.Sci.USA 102 Pages: 12365-12370
• [Presentation] The Arf-like GTPase Ar18 is required for lysosome biogenesis in C.elezans. (2009)
  • Author(s): Katada T., et al.
  • Organizer: 国際生理学会IUPS2009シンポジウム
  • Place of Presentation: 京都・京都国際会館
  • Year and Date: 2009-07-28
• [Presentation] Functional analysis of the Arf-like, small GTPase Arl8 in lysosomes. (2007)
  • Author(s): Kontani K, et. al.
  • Organizer: 第30回日本分子生物学会・第80回日本生化学会
  • Place of Presentation: 横浜
  • Year and Date: 2007-12-13
• [Book] 薬学生のための細胞生物学 (2005)
  • Author(s): 堅田利明
  • Total Pages: 227
  • Publisher: 廣川書店
• [Remarks]
  • URL: http://www.md.tsukuba.ac.jp/basic-med/biochem/kanaholab/Gprotein/g/index.html
• [Remarks]
  • URL: http://www.f.u-tokyo.ac.jp/~seiri
• [Remarks] 特定領域研究ホームページ
  • URL: http://www.md.tsukuba.ac.jp/basic-med/biochem/kanaholab/Gprotein/g/index.html
• [Remarks]
  • URL: http://www.md.tsukuba.ac.jp/basic-med/biochem/kanaholab/Gprotein/g/index.html
• [Remarks]
  • URL: http://www.f.u-tokyo.ac.jp/~seiri
• [Remarks]
  • URL: http://www.md.tsukuba.acjp/basic-med/biochem/kanahol.ab/Gprotein/g/index.html
• [Remarks]
  • URL: http://www.f.u-tokyo.acjp/~seiri
• [Remarks]
  • URL: http://www.md.tsukuba.ac.jp/basic-med/biochem/kanaholab/Gprotein/g/index.html
• [Remarks]
  • URL: http://www.f.u-tokyo.ac.jp/~seiri