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Physiological regulation of G protein signaling by RGS proteins

Research Project

Project/Area Number 17079005
Research Category

Grant-in-Aid for Scientific Research on Priority Areas

Allocation TypeSingle-year Grants
Review Section Biological Sciences
Research InstitutionOsaka University

Principal Investigator

KURACHI Yoshihisa  Osaka University, 医学系研究科, 教授 (30142011)

Co-Investigator(Kenkyū-buntansha) INANOBE Atsushi  大阪大学, 医学系研究科, 准教授 (00270851)
MURAKAMI Shingo  大阪大学, 医学系研究科, 助教 (40437314)
FURUTANI Kazuharu  大阪大学, 医学系研究科, 助教 (40452437)
ISHII Masaru  大阪南医療センター(臨床研究部), 免疫異常疾患研究所, 研究員 (10324758)
Project Period (FY) 2005 – 2009
Project Status Completed (Fiscal Year 2009)
Budget Amount *help
¥72,900,000 (Direct Cost: ¥72,900,000)
Fiscal Year 2009: ¥8,900,000 (Direct Cost: ¥8,900,000)
Fiscal Year 2008: ¥16,000,000 (Direct Cost: ¥16,000,000)
Fiscal Year 2007: ¥16,000,000 (Direct Cost: ¥16,000,000)
Fiscal Year 2006: ¥23,100,000 (Direct Cost: ¥23,100,000)
Fiscal Year 2005: ¥8,900,000 (Direct Cost: ¥8,900,000)
KeywordsG蛋白質 / RGS蛋白質 / 細胞内シグナル / カルシウムシグナル / カリウムチャネル / 数理モデル / シミユレーション / イオンチャネル / シミュレーション / 心臓生理 / 脂質代謝 / 脂質ラフト
Research Abstract

Trimeric G proteins transmit extracellular signals to regulate the intracellular effectors. The spatiotemporal regulation of this signal closely links to the cellular responses. In this study, we addressed the regulation of G protein signaling by RGS proteins, the mathematical models of G protein signaling and the structure-function relationship of K^+ channel to analyze the physiological regulation of G protein signaling.

Report

(6 results)
  • 2009 Annual Research Report   Final Research Report ( PDF )
  • 2008 Annual Research Report
  • 2007 Annual Research Report
  • 2006 Annual Research Report
  • 2005 Annual Research Report
  • Research Products

    (64 results)

All 2010 2009 2008 2007 2006 2005

All Journal Article (56 results) (of which Peer Reviewed: 43 results) Presentation (2 results) Book (6 results)

  • [Journal Article] experiments and simulation to develop a physiological model of G-protein control of muscarinic K+ channels in mammalian atrial cells.2010

    • Author(s)
      Murakami S, Suzuki S, Ishii M, Inanobe A, Kurachi Y. Cellular modeling
    • Journal Title

      Phil Trans Roy Soc A. (in press)

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] On calcium-buffer dynamics within the excess buffer regime.2010

    • Author(s)
      Ait-Haddou R, Kurachi Y, Nomura T.
    • Journal Title

      J. Theor. Biol. Vol264

      Pages: 55-65

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Experiments and simulation to develop a physiological model of G-prote in control of muscarinic2010

    • Author(s)
      Murakami, S., et al.
    • Journal Title

      Phil.Trans.Roy.Soc.A (In press)

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] On calcium-buffer dynamics within the excess buffer regime.2010

    • Author(s)
      Ait-Haddou, R., et al.
    • Journal Title

      J.Theor.Biol. (In press)

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A platform for in silico modeling of physiological systems III.2009

    • Author(s)
      Suzuki Y, Kurachi Y, Nomura T. (10名省略, 8番目)
    • Journal Title

      Conf Proc IEEE Eng Med Biol Soc. Vol2009

      Pages: 2803-2806

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Opening velocity, a novel parameter, for finger tapping test in patients with Parkinson's disease.2009

    • Author(s)
      Yokoe M, Kurachi Y, Sakoda S. (3名省略, 4番目)
    • Journal Title

      Parkinsonism Relat. Disord. Vol15

      Pages: 440-444

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Optimal treatment strategy for patients with paroxysmal atrial fibrillation: J-RHYTHM Study.2009

    • Author(s)
      Ogawa S, Kurachi Y, Mitamura H. (21名省略, 13番目)
    • Journal Title

      Circ. J. Volxxxxxxx

      Pages: 242-248

    • NAID

      110007042201

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] A platform for in silico modeling of physiological systems III.2009

    • Author(s)
      Suzuki, Y., et al.
    • Journal Title

      Conf.Proc.IEEE.Eng.Med.Biol.Soc. 2009

      Pages: 2803-2806

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Optimal treatment strategy for patients with paroxysmal atrial fibrillation : J-RHYTHM Study.2009

    • Author(s)
      Ogawa, S., et al.
    • Journal Title

      Circ.J. 2009

      Pages: 242-248

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Opening velocity, a novel parameter, for finger tapping test in patients with Parkinson's disease2009

    • Author(s)
      Yokoe, M., et.al.
    • Journal Title

      Parkinsonism Relat. Disord (In press)

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] In silico prediction of the chemical block and human ether-a-go-go-related gene (hERG) K+ current.2008

    • Author(s)
      Inanobe A, Kamiya N, Murakami S, Fukunishi Y, Nakamura H, Kurachi Y.
    • Journal Title

      J Physiol Sci. Vol58

      Pages: 459-470

    • NAID

      10027710790

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Specifications of insilicoML 1. 0: A multilevel biophysical model description languate.2008

    • Author(s)
      Asai Y, Kurachi Y, Nomura T. (7名省略, 9番目)
    • Journal Title

      J Physiol Sci. Vol58

      Pages: 447-458

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Microstructure-based monte carlo simulation of Ca2+ dynamics evoking cardiac calcium channel inactivation.2008

    • Author(s)
      Kawazu T. Murakami S, Adachi-Akahane S, Findlay I, Ait-Haddou R, Kurachi Y, Nomura T.
    • Journal Title

      J Physiol Sci. Vol58

      Pages: 471-480

    • NAID

      10027710920

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Meeting report on the 2nd MEI international symposium - the worldwide challenge to physiome and systems biology and Osaka accord -2008

    • Author(s)
      Hunter P, Kurachi Y, Nobel D, Viceconti M.
    • Journal Title

      J Physiol Sci. Vol58

      Pages: 425-431

    • NAID

      130004466944

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] A platform for in silico modeling of physiological systems II. CellML compatibility and other extended capabilities.2008

    • Author(s)
      Suzuki Y, Kurachi Y, Nomura T. (6名省略, 8番目)
    • Journal Title

      Conf Proc IEEE Eng Med Biol Soc. Vol2008

      Pages: 573-576

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Evaluation of brain and whole-body pharmacokinetics of 11C-labeled diphenylhydantoin in rats by means of planar positron imaging system.2008

    • Author(s)
      Hasegawa Y, Kurachi Y, Hatazawa J. (5名省略, 7番目)
    • Journal Title

      Ann Nucl Med. Vol22

      Pages: 301-307

    • NAID

      10021281082

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] In silico risk assessment for drug-induction of cardiac arrhythmia.2008

    • Author(s)
      Suzuki S, Murakami S, Tsujimae K, Findlay I, Kurachi Y.
    • Journal Title

      Prog Biophys Mol Biol. Vol98

      Pages: 52-60

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] In silico study on the effects of IKur block kinetics on prolongation of human action potential after atrial fibrillation-induced electrical remodeling.2008

    • Author(s)
      Tsujimae K. Murakami S, Kurachi Y.
    • Journal Title

      Am J Physiol Heart Circ Physiol. Vol294

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] A platform for in silico modeling of physiological systems II. CeIIML compatibility and other extended capabilities2008

    • Author(s)
      Suzuki, Y., et.al.
    • Journal Title

      Conf. Proc. IEEE. Eng. Med. Biol. Soc 2008

      Pages: 573-576

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] In silico prediction of the chemical block and human ether-a-go-go-related gene(hERG)K+ current2008

    • Author(s)
      Inanobe, A., et.al.
    • Journal Title

      J. Physiol. Sci 58

      Pages: 459-470

    • NAID

      10027710790

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Specifications of insilicoML 1.0 : A multilevel biophysical model description languate2008

    • Author(s)
      Asai, Y., et.al.
    • Journal Title

      J. Physiol. Sci 58

      Pages: 447-458

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Microstructure-based monte carlo simulation of Ca2+ dynamics evoking cardiac calcium channel inactivation2008

    • Author(s)
      Kawazu, T., et.al.
    • Journal Title

      J. Physiol. Sci 58

      Pages: 471-480

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Meeting report on the 2^<nd> MEI international symposium-the worldwide challenge to physiome and systems biology and Osaka accord-2008

    • Author(s)
      Hunter, P., et.al.
    • Journal Title

      J. Physiol. Sci 58

      Pages: 425-431

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] In silico risk assessment for drug-induction of cardiac arrhythmia2008

    • Author(s)
      Suzuki, S., et.al.
    • Journal Title

      Prog. Biophys. Mol. Biol 98

      Pages: 52-60

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Evaluation of brain and whole-body pharmacokinetics of 11C-labeled dip henylhydantoin in rats by means of planar positron imaging system2008

    • Author(s)
      Hasegawa, Y., et.al.
    • Journal Title

      Ann. Nucl. Med 22

      Pages: 301-307

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] In silico study on the effects of IKur block kinetics on prolongation of human action potential after atrial fibrillation-induced electrical remodeling.2008

    • Author(s)
      Tsujimae, K
    • Journal Title

      Am J Physiol Heart Circ Physiol. 294

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Mutational analysis of block and facilitation of HERG current by a class III anti-arrhythmic agent, nifekalant.2007

    • Author(s)
      Hosaka Y, Inanobe A, (9名省略, 10番目) Kurachi Y. (12番目)
    • Journal Title

      Channels Vol1

      Pages: 198-208

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Characterization of a novel ATP-sensitive K+ channel opener, 4-methyl-N-(2, 2, 2-trichloro-1-(3-pyridin-3-ylthio ureido)ethyl) benzamide (A-251179) on urinary bladder relaxation and cystometric parameters.2007

    • Author(s)
      Shieh C-C, Kurachi Y, Gopalakrishnan M. (11名省略, 13番目)
    • Journal Title

      Br J Pharmacol. Vol151

      Pages: 467-475

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Frequency dependent effects of various IKr blockers on cardiac action potential duration in a human atrial model.2007

    • Author(s)
      Tsujimae K, Suzuki S, Murakami S, Kurachi Y.
    • Journal Title

      Am J Physiol Heart Circ Physiol. Vol297

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Anti-KCNH2 antibody-induced long QT syndrome: novel acquired form of long QT syndrome.2007

    • Author(s)
      Nakamura K, Kurachi Y, Ohe T. (12名省略, 14番目)
    • Journal Title

      J. Am. Coll. Cardiol. Vol50

      Pages: 1808-1809

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Structural diversity in the cytoplasmic region of G protein-gated inward rectifier K+ channels.2007

    • Author(s)
      Inanobe A, Matsuura T, Nakagawa A, Kurachi Y.
    • Journal Title

      Channels Vol1

      Pages: 39-45

    • NAID

      130005449261

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Anti-KCNH2 antibody-induced long QT syndrome: novel acquired form of long QT syndrome.2007

    • Author(s)
      Nakamura, K
    • Journal Title

      J Am Coll Cardiol. 50

      Pages: 1808-1809

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Frequency dependent effects of various IKr blockers on cardiac action potential duration in a human atrial model.2007

    • Author(s)
      Tsujimae, K
    • Journal Title

      Am J Physiol Heart Circ Physiol. 297

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Structural diversity in the cytoplasmic region of G protein-gated inward rectifier K^+ channels.2007

    • Author(s)
      Inanobe, A.
    • Journal Title

      Channels (In press)

    • NAID

      130005449261

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Characterization of a novel ATP-sensitive K+ channel opener, 4-methl-N-(2, 2, 2-trichloro-1-(3-pyridin-3-ylthioureido) ethyl) benzamide (A-251179) on urinary bladder relaxation and cystometric parameters.2007

    • Author(s)
      Shieh, C-C.
    • Journal Title

      Brit. J. Pharmacol. (In press)

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Pigment pattern in jaguar/obelix zebrafish is caused by a Kir7. 1 mutation: implications for the regulation of melanosome movement.2006

    • Author(s)
      Iwashita M, Kurachi Y, Kondo S. (5名省略, 6番目)
    • Journal Title

      PLoS Genet. Vol2

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Muscarinic acetylcholine receptors.2006

    • Author(s)
      Ishii M, Kurachi Y.
    • Journal Title

      Curr Pharm Des. Vol12

      Pages: 3573-3581

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Randomized study of angiotensin II type 1 receptor blocker vs dihydropiridine calcium antagonist for the treatment of paroxysmal atrial fibrillation in patients with hypertension.2006

    • Author(s)
      Yamashita T, Kurachi Y, Watanabe E. (21名省略, 12番目)
    • Journal Title

      Circ J. Vol70

      Pages: 1318-1321

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Spot pattern of leopard Danio is caused by mutation in the zebrafish connexin41.8 gene.2006

    • Author(s)
      Watanabe M, Iwashita M, Ishii M, Kurachi Y, Kawakami A, Kondo A, Okada N.
    • Journal Title

      EMBO Rep. Vol7

      Pages: 893-897

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Carvedilol blocks cardiac KATP and KG but not IK1 channels by acting at the bundle-crossing regions.2006

    • Author(s)
      Kikuta J, Ishii M, Kishimoto K, Kurachi Y.
    • Journal Title

      Eur J Pharmacol. Vol529

      Pages: 47-54

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Muscarinic acetylcholine receptors.2006

    • Author(s)
      Ishii, M.
    • Journal Title

      Curr. Pharm. Des. 12巻・28号

      Pages: 3573-3581

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Pigment pattern in jaguar/obelix zebrafish is caused by a Kir7.1 mutation : implications for the regulation of melanosome movement.2006

    • Author(s)
      Iwashita, M.
    • Journal Title

      PLoS Genet. 2巻・11号

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Randomized study of angiotensin II type 1 receptor blocker vs dihydropiridine calcium antagonist for the treatment of paroxysmal atrial fibrillation in patients with hypertension.2006

    • Author(s)
      Yamashita, T.
    • Journal Title

      Circ. J. 70巻・10号

      Pages: 1318-1321

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Spot pattern of leopard Danio is caused by mutation in the zebrafish connexin4l.8 gene.2006

    • Author(s)
      Watanabe, M.
    • Journal Title

      EMBO-Rep. 7巻・9号

      Pages: 893-897

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Carvedilol blocks cardiac K_<ATP> and K_G but not I_<K1> channels by acting at the bundle-crossing regions.2006

    • Author(s)
      Kikuta, J.
    • Journal Title

      Eur J Pharmacol. 529・1

      Pages: 47-54

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Molecular and cellular diversity of neuronal G-protein-gated potassium channels.2005

    • Author(s)
      Koyrakh I, Kurachi Y, Wickman K. (4名省略, 5番目)
    • Journal Title

      J Neurosci. Vol25

      Pages: 11468-11478

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] In vivo interaction between RGS4 and calmodulin visualized with FRET techniques: possible involvement of lipid raft.2005

    • Author(s)
      Ishii M, Ikushima M, Kurachi Y.
    • Journal Title

      Biochem Biophys Res Commun. Vol338

      Pages: 839-846

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Gestational change of K+ channel opener effect is correlated with the expression of uterine KATP channel subunits.2005

    • Author(s)
      Sawada K, Kurachi Y. (5名省略, 7番目)
    • Journal Title

      Eur J Obstet Gynecol Reprod Biol. Vol122

      Pages: 49-56

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] A functional role of the C-terminal 42 amino acids of SUR2A and SUR2B in the physiology and pharmacology of cardiovascular ATP-sensitive K+ channels.2005

    • Author(s)
      Yamada M, Kurachi Y.
    • Journal Title

      J Mol Cell Cardiol. Vol39

      Pages: 1-6

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Phosphatidylinositol 3, 4, 5-trisphosphate and Ca2+/calmodulin competitively bind to the regulators of G-protein-signalling (RGS) domain of RGS4 and reciprocally regulate its action.2005

    • Author(s)
      Ishii M, Fujita S, Yamada M, Hosaka Y, Kurachi Y.
    • Journal Title

      Biochem J. Vol385

      Pages: 65-73

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] In vivo interaction between RGS4 and calmodulin visualized with FRET techniques : possible involvement of lipid raft.2005

    • Author(s)
      Ishii, M.
    • Journal Title

      Biochem Biophys Res Commun. 338・2

      Pages: 839-846

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Phosphatidylinositol 3,4,5-trisphosphate and Ca^<2+>/calmodulin competitive bind to the regulators of G-protein-signalling (RGS) domain of RGS4 and reciprocally regulate its action.2005

    • Author(s)
      Ishii, M.
    • Journal Title

      Biochem J. 385・1

      Pages: 65-73

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Molecular and cellular diversity of neuronal G-protein-gated potassium channels.2005

    • Author(s)
      Koyrakh, I.
    • Journal Title

      J.Neurosci. 25・49

      Pages: 11468-11478

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Gestational change of K^+ channel opener effect is correlated with the expression of uterine K_<ATP> channel subunits.2005

    • Author(s)
      Sawada, K.
    • Journal Title

      Eur J Obstet Gynecol Reprod Biol. 122・1

      Pages: 49-56

    • Related Report
      2005 Annual Research Report
  • [Journal Article] A functional role of the C-terminal 42 amino acids of SUR2A and SUR2B in the physiology and pharmacology of cardiovascular ATP-sensitive K^+ channels.2005

    • Author(s)
      Yamada, M.
    • Journal Title

      J Mol Cell Cardiol 39・1

      Pages: 1-6

    • Related Report
      2005 Annual Research Report
  • [Journal Article] International Union of Pharmacology. LIV. Nomenclature and molecular relationships of inwardly rectifying potassium channels.2005

    • Author(s)
      Kubo, Y.
    • Journal Title

      Pharmacol.Rev. 57・4

      Pages: 509-526

    • Related Report
      2005 Annual Research Report
  • [Presentation] Structural Insights into the Function of Cytoplasmic Region of G Protein-Gated Inward Rectifier K+ channel.2009

    • Author(s)
      Inanobe A.
    • Organizer
      International Congress of Physiological Sciences.
    • Place of Presentation
      京都
    • Year and Date
      2009-07-30
    • Related Report
      2009 Final Research Report
  • [Presentation] Structural Insights into the Function of Cytoplasmic Region of G Protein-Gated Inward Rectifier K^+ channel2009

    • Author(s)
      Inanobe, A.
    • Organizer
      International Congress of Physiological Sciences
    • Place of Presentation
      京都
    • Year and Date
      2009-07-30
    • Related Report
      2009 Annual Research Report
  • [Book] 細胞工学(NMDA受容体の動的構造変化:結晶構造解析によるアプローチ)2006

    • Author(s)
      稲野辺厚
    • Publisher
      秀潤社
    • Related Report
      2009 Final Research Report
  • [Book] 別冊・医学の歩み(イオンチャネルの構造と機能)2005

    • Author(s)
      稲野辺厚, 倉智嘉久
    • Publisher
      医歯薬出版
    • Related Report
      2009 Final Research Report
  • [Book] 別冊・医学の歩み(イオンチャネルの構造と機能)2005

    • Author(s)
      保坂幸男, 倉智嘉久
    • Publisher
      医歯薬出版
    • Related Report
      2009 Final Research Report
  • [Book] 別冊・医学の歩み( HERGチャネルとKCNQ1/KCNE1チャネル)2005

    • Author(s)
      石井優, 倉智嘉久
    • Publisher
      医歯薬出版
    • Related Report
      2009 Final Research Report
  • [Book] イオンチャネル最前線update2005

    • Author(s)
      稲野辺 厚
    • Total Pages
      14
    • Publisher
      医歯薬出版株式会社
    • Related Report
      2005 Annual Research Report
  • [Book] イオンチャネル最前線update2005

    • Author(s)
      石井 優
    • Total Pages
      7
    • Publisher
      医歯薬出版株式会社
    • Related Report
      2005 Annual Research Report

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Published: 2005-04-01   Modified: 2018-03-28  

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