Links Between S-phase and M-phase Mediated by Fork Protection Complex

• Project/Area Number: 17080001
• Research Category: Grant-in-Aid for Scientific Research on Priority Areas
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: Tokyo Institute of Technology
• Principal Investigator: SHIRHIGE Katsuhiko Tokyo Institute of Technology, 大学院・生命理工学研究科, 連携教授 (90273854)
• Project Period (FY): 2005 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥84,900,000 (Direct Cost: ¥84,900,000); Fiscal Year 2009: ¥17,000,000 (Direct Cost: ¥17,000,000); Fiscal Year 2008: ¥25,500,000 (Direct Cost: ¥25,500,000); Fiscal Year 2007: ¥17,000,000 (Direct Cost: ¥17,000,000); Fiscal Year 2006: ¥12,700,000 (Direct Cost: ¥12,700,000); Fiscal Year 2005: ¥12,700,000 (Direct Cost: ¥12,700,000)
• Keywords: DNAチップ / 染色体分配 / 染色体動態 / 染色体複製 / 複製チェックポイント / ヘリカーゼ / MCM複合体 / チェックポイント因子 / ラギング鎖 / リーディング鎖 / ポリメラーゼ / 複製停止

Research Abstract

DNA replication checkpoint plays a crucial role for the maintenance of genome integrity. With the help of research grant, we have found that replication checkpoint mediator complex (that consists of Tof1, Mrc1, and Csm3) is a key linker between DNA polymerase and helicase. This function as a linker is important for proper mediator function to sense different types of replication stress for the activation of the DNA replication checkpoint and protection of the replisome.

Research Products

• [Journal Article] SCF(Dia2) regulates DNA replication forks during S-phase in budding yeast. (2009)
  • Author(s): S. Mimura, M. Komata, T. Kishi, K. Shirahige, T. Kamura
  • Journal Title: EMBO J. 28 Pages: 3693-3705
  • Peer Reviewed
• [Journal Article] Csm3, Tof1, and Mrc1 form a heterotrimeric mediator complex that associates with DNA replication forks. (2009)
  • Author(s): M. B, o, Y.M. Katou, M. Komata, H. Tanaka, T. Itoh, T. Sutani,, K. Shirahige
  • Journal Title: J Biol Chem. 284 Pages: 34355-34365
  • Peer Reviewed
• [Journal Article] ChIP-on-Chip Analysis of DNA Topoisomerases. (2009)
  • Author(s): R. Bermejo, Y.M. Katou, K. Shirahige,, M. Foiani
  • Journal Title: Methods Mol Biol. 582 Pages: 103-118
• [Journal Article] Involvement of a chromatin remodeling complex in damage tolerance during DNA replication Nat. (2009)
  • Author(s): K.B. Falbo*, C. Alabert*, Y. Katou*, S. Wu, J. Han, T. Wehr, J. Xiao, X. He, Z. Zhang, Y. Shi, K. Shirahige, P. Pasero,, X. Shen
  • Journal Title: Struct. Mol. Biol. 16 Pages: 1167-1172
  • Peer Reviewed
• [Journal Article] Genome-Organizing Factors Top2 and Hmo1 Prevent Chromosome Fragility at Sites of S phase Transcription Cell (2009)
  • Author(s): R. Bermejo, T. Capra, V. Gonzales-Huici, D. Fachinetti, A. Cocito, G. Natoli, Y. Katou, H. Mori, K. Kurokawa, K. Shirahige,, M. Foiani
  • Journal Title: 138 Pages: 870-884
  • Peer Reviewed
• [Journal Article] The Direct Binding of Mrc1, a Checkpoint Mediator, to Mcm6, a Replication Helicase, is Essential for Replication Checkpoint Against Methyl Methanesulfonate-induced Stress. (2009)
  • Author(s): M. Komata, M. B, o, H. Araki,, K. Shirahige
  • Journal Title: Mol. Cell. Biol. 29 Pages: 5008-5019
  • Peer Reviewed
• [Journal Article] Rec8 guides canonical Spo11 distribution along yeast meiotic chromosomes. (2009)
  • Author(s): K. Kugou, T. Fukuda, S. Yamada, M. Ito, H. Sasanuma, S. Mori, Y. Katou, T. Itoh, K. Matsumoto, T. Shibata, K. Shirahige,, K. Ohta
  • Journal Title: Mol Biol Cell. 20 Pages: 3064-3076
  • Peer Reviewed
• [Journal Article] Ctf4 coordinates the progression of helicase and DNA polymerase alpha. (2009)
  • Author(s): H. Tanaka, Y. Katou, M. Yagura, K. Saitoh, T. Itoh, H. Araki, M. B, o#,, K. Shirahige#
  • Journal Title: Genes Cells. 14 Pages: 807-820
  • Peer Reviewed
• [Journal Article] Budding Yeast Wpl1(Rad61)-Pds5 Complex Counteracts Sister Chromatid Cohesion- Establishing Reaction. (2009)
  • Author(s): T. Sutani, T. Kawaguchi, R. Kanno, T. Itoh,, K. Shirahige
  • Journal Title: Curr. Biol. 19 Pages: 492-497
  • Peer Reviewed
• [Journal Article] The Direct Binding of Mrc1, a Checkpoint Mediator, to Mcm6, a Replication Helicase, is Essential for Replication Checkpoint Against Methyl Methanesulfonate-induced Stress (2009)
  • Author(s): M.Komata, M.Bando, H.Araki, K.Shirahige
  • Journal Title: Mol Biol Cell. 29 Pages: 5008-5019
  • Peer Reviewed
• [Journal Article] Involvement of a chromatin remodeling complex in damage tolerance during DNA replication (2009)
  • Author(s): K.B.Falbo, C.Alabert, Y.Katou, S.Wu, J.Han, T.Wehr, J.Xiao, X.He, Z.Zhang, Y.Shi, K.Shirahige, P.Pasero, X.Shen
  • Journal Title: Nat.Struct.Mol.Biol. 16 Pages: 1167-1172
  • Peer Reviewed
• [Journal Article] SCF(Dia2)regulates DNA replication forks during S-phase in budding yeast (2009)
  • Author(s): S.Mimura, M.Komata, T.Kishi, K.Shirahige, T.Kamura
  • Journal Title: Embo J 28 Pages: 3693-3705
  • Peer Reviewed
• [Journal Article] H. Tanaka#, Y. Katou#, M. Yagura, K. Saitoh, T. Itoh, H. Araki, M. Bando* and K. Shirahige* (2009)
  • Author(s): H. Tanaka#, Y. Katou#, M. Yagura, K. Saitoh, T. Itoh, H. Araki, M. Bando* and K. Shirahige*
  • Journal Title: Genes Cells (In press)
  • Peer Reviewed
• [Journal Article] Rad51 suppresses gross chromosomal rearrangement at centromere in Schizosaccharomyces pombe. (2008)
  • Author(s): K. Nakamura, A. Okamoto, Y. Katou, C. Yadani, T. Shit, a, C. Kaweeteerawat, T.S. Takahashi, T. Itoh, K. Shirahige, H. Masukata,, T. Nakagawa
  • Journal Title: EMBO J. 27 Pages: 3036-3046
  • Peer Reviewed
• [Journal Article] Balance between distinct HP1 family proteins controls heterochromatin assembly in fission yeast. (2008)
  • Author(s): M. Sadaie, R. Kawaguchi, Y. Ohtani, F. Arisaka, K. Tanaka, K. Shirahige, J. Nakayama
  • Journal Title: Mol Cell Biol. 28 Pages: 6973-6988
  • Peer Reviewed
• [Journal Article] polymeraseεfunction together in linking DNA replication and the S phase checkpoint Mol. (2008)
  • Author(s): H. Lou, M. Komata, Y. Katou, Z. Guan, C.C. Reis, M. Budd, K. Shirahige, J.L. Campbell1, Mrc1, DNA
  • Journal Title: Cell 32 Pages: 106-117
  • Peer Reviewed
• [Journal Article] Nutrient-regulated antisense and intragenic RNAs modulate a signal transduction pathway in yeast (2008)
  • Author(s): M. Nishizawa, T. Komai, Y. Katou, K. Shirahize, T. Itoh, and A. Toh-E
  • Journal Title: PLoS Biol 6 Pages: 2817-2830
  • Peer Reviewed
• [Journal Article] Balance between distinct HP1 family proteins controls heterochromatin assembly in fission yeast (2008)
  • Author(s): M. Sadaie, R. Kawaguchi, Y. Ohtani,F. Aiisaka, K. Tanaka, K. Shirahige, J. Nakayama
  • Journal Title: Mol Cell Biol 28 Pages: 6973-6988
  • Peer Reviewed
• [Journal Article] A DNA polymerase alpha accessory protein, Mcll, is required for propagation of centromere structures in fission yeast (2008)
  • Author(s): T. Natsume, Y. Tsutsui, T. Sutani, E. M. Dunleavy, A.L. Pidoux, H. Iwasaki, K. Shirahige, R.C. Allshire, F. Yamao
  • Journal Title: PLoS ONE 3
  • Peer Reviewed
• [Journal Article] A DNA Polymerase {lower case alpha} Accessory Protein, Mcll, is Required for Propagation of Centromere Structures in Fission Yeast. (2008)
  • Author(s): Toyoaki Natsume, Yasuhiro Tsutsui, Takashi Sutani, Elaine M.Dunleavy, Alison L. Pidoux, Hiroshi Iwasaki, Katsuhiko Shirahige, Robin C. Allshire, Fumiaki Yamao
  • Journal Title: PLoS One In press
  • Peer Reviewed
• [Journal Article] Abnormality in initiation program of DNA replication is monitored by the highly repetitive rDNA array on chromosome XII in budding yeast, Mol. (2007)
  • Author(s): S. Ide, K. Watanabe, H. Watanabe, K. Shirahige, T. Kobayashi,, H. Maki
  • Journal Title: Cell. Biol. 27 Pages: 568-578
  • Peer Reviewed
• [Journal Article] Genome-wide localization of pre-RC sites and identification of replication origins in fission yeast. (2007)
  • Author(s): M. Hayashi, Y. Katou, T. Itoh, M. Tazumi, Y. Yamada, T. Takahashi, T. Nakagawa, K. Shirahige,, H. Masukata
  • Journal Title: EMBO J. 26 Pages: 1327-1339
  • Peer Reviewed
• [Journal Article] Displacement and re-accumulation of centromeric cohesin during transient pre-anaphase centromere splitting. (2007)
  • Author(s): M.T. Ocampo-Hafalla, Y. Katou, K. Shirahige, F. Uhlmann
  • Journal Title: Chromosoma. 116 Pages: 531-544
  • Peer Reviewed
• [Journal Article] Displacement and re-accumulation of centromeric cohesin during transient pre-anaphase centromere splitting. (2007)
  • Author(s): Ocampo-Hafalla MT, Katou Y, Shirahige K, Uhimann F.
  • Journal Title: Chromosoma 116 Pages: 531-544
  • Peer Reviewed
• [Journal Article] Mrell mediates gene regulation in yeast spore development, (2007)
  • Author(s): Kugou K, Sasanuma H, Matsumoto K, Shirahige K, Ohta K.
  • Journal Title: Genes Genet Syst 82 Pages: 21-33
  • Peer Reviewed
• [Journal Article] Genome-wide localization of pre-RC sites and identification of replication origins in fission yeast. (2007)
  • Author(s): Hayashi M, Katou Y, Itoh T, Tazumi A, Yamada Y, Takahashi T, Nakagawa T, Shirahige K, Masukata H.
  • Journal Title: EMBO J. 26 Pages: 1327-1339
  • Peer Reviewed
• [Journal Article] Abnormality in initiation program of DNA replication is monitored by the highly repetitive rDNA array on chromosome XII in budding yeast (2007)
  • Author(s): S.Ide, K.Watanabe, H.Watanabe, K.Shirahige, T.Kobayashi, H.Maki
  • Journal Title: Mol. Cell. Biol. 27 Pages: 568-578
• [Journal Article] Perturbation of the activity of replication origin by meiosis specific transcription (2007)
  • Author(s): S.Mori, K.Shirahige
  • Journal Title: J. Biol. Chem. 282 Pages: 4447-4452
• [Journal Article] Meiotic recombination-related DNA synthesis and its implications for cross-over and non-cross-over recombinant formation. (2007)
  • Author(s): M.Terasawa, H.Ogawa, Y.Tsukamoto, Shinohara, K.Shirahige, N.Kleckner, T.Ogawa
  • Journal Title: Proc Natl Acad Sci U S A. Mar 23
• [Journal Article] Meiotic cohesins modulate chromosome compaction during meiotic prophase in fission yeast. (2006)
  • Author(s): D.Q. Ding, N. Sakurai, Y. Katou, T. Itoh, K. Shirahige, T. Haraguchi,, Y. Hiraoka.
  • Journal Title: J Cell Biol. 174 Pages: 499-508
  • Peer Reviewed
• [Journal Article] Establishment of Sister Chromatid Cohesion at the S. cerevisiae Replication Fork. (2006)
  • Author(s): A. Lengronne, J. McIntyre, Y. Katou, Y. Kanoh, K.P. Hopfner, K. Shirahige,, F. Uhlmann
  • Journal Title: Mol Cell. 23 Pages: 787-799
  • Peer Reviewed
• [Journal Article] Genomic Approach for the Understanding of Dynamic Aspect of Chromosome Behavior. (2006)
  • Author(s): Y. Katou, K. Kaneshiro, H. Aburatani,, K. Shirahige
  • Journal Title: Methods in Enzymology, Elsevier Life Sciences (CA), vol. 409, chapter 23 Pages: 389-410
• [Journal Article] Different requirements for the association of ATR-ATRIP and 9-1-1 to the stalled replication forks. (2006)
  • Author(s): Y. Kanoh, K. Tamai,, K. Shirahige.
  • Journal Title: Gene. 377 Pages: 88-95
  • Peer Reviewed
• [Journal Article] Different requirements for the association of ATR-ATRIP and 9-1-1 to the stalled replication forks. (2006)
  • Author(s): Y.Kanoh, K.Tamai, K.Shirahige
  • Journal Title: Gene 377 Pages: 88-95
• [Journal Article] Establishment of Sister Chromatid Cohesion at the S. cerevisiae Replication Fork. (2006)
  • Author(s): A.Lengronne, J.McIntyre, Y.Katou, Y.Kanoh, K.P.Hopfner, K.Shirahige, F.Uhlmann
  • Journal Title: Mol Cell. 23 Pages: 787-799
• [Journal Article] Evidence that Loading of Cohesin Onto Chromosomes Involves Opening of Its SMC Hinge (2006)
  • Author(s): S.Gruber, P.Arumugam, Y.Katou, D.Kuglitsch, W.Helmhart, K.Shirahige, K.Nasmyth
  • Journal Title: Cell 127 Pages: 523-537
• [Journal Article] Protein Phosphatase 2A protects centromeric sister chromatid cohesion. (2006)
  • Author(s): C.G.Riedel, V.L.Katis, Y.Katou, S.Mori, W.Helmhart, M.Galova, M.Petronczki, J.Gregan, B.Cetin, I.Mudrak, E.Ogris, K.Mechtler, L.Pelletier, F.Buchholz, K.Shirahige, K.Nasmyth
  • Journal Title: Nature (article) (In press)
• [Journal Article] A checkpoint control linking meiotic S phase and recombination initiation in fission yeast. (2005)
  • Author(s): Y. Tonami, H. Murakami, K. Shirahige,, M. Nakanishi.
  • Journal Title: Proc. Natl. Acad. Sci. U.S.A. 102 Pages: 5797-801
  • Peer Reviewed
• [Presentation] Role of Cohesin acetylation in transcription (2010)
  • Author(s): 白髭克彦
  • Organizer: International Conference on Radiation and Cancer Biology at Nagasaki 2010
  • Place of Presentation: Nagasaki University Medical School, Matsumoto Ryojun Auditotturn, Bauduin Hall
  • Year and Date: 2010-02-20
• [Presentation] 複製と分配の連携機構の解明 (2009)
  • Author(s): 白髭克彦
  • Organizer: 遺伝学会シンポジウム
  • Place of Presentation: 信州大学
  • Year and Date: 2009-11-10
• [Remarks]
  • URL: http://www.iam.u-tokyo.ac.jp/chromosomeinformatics/index.html