• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Molecular mechanism of microbial DNA partitioning : structure and function

Research Project

Project/Area Number 17080009
Research Category

Grant-in-Aid for Scientific Research on Priority Areas

Allocation TypeSingle-year Grants
Review Section Biological Sciences
Research InstitutionNational Institute of Genetics

Principal Investigator

NIKI Hironori  National Institute of Genetics, 系統生物研究センター, 教授 (70208122)

Co-Investigator(Kenkyū-buntansha) TSUTSUI Yasuhiro  国立遺伝学研究所, 分子遺伝研究系, 助教 (00390625)
KIMURA Akatsuki  国立遺伝学研究所, 新分野創造センター, 准教授 (10365447)
FURUYA Kanji  国立遺伝学研究所, 系統生物研究センター, 助教 (90455204)
Project Period (FY) 2005 – 2009
Project Status Completed (Fiscal Year 2009)
Budget Amount *help
¥100,500,000 (Direct Cost: ¥100,500,000)
Fiscal Year 2009: ¥20,100,000 (Direct Cost: ¥20,100,000)
Fiscal Year 2008: ¥20,100,000 (Direct Cost: ¥20,100,000)
Fiscal Year 2007: ¥20,100,000 (Direct Cost: ¥20,100,000)
Fiscal Year 2006: ¥20,100,000 (Direct Cost: ¥20,100,000)
Fiscal Year 2005: ¥20,100,000 (Direct Cost: ¥20,100,000)
Keywords染色体 / 分配 / DNA結合 / 無核 / 細胞分裂 / バクテリア / セントロメア / SopAタンパク質 / MreBタンパク質 / 無核細胞 / 核様体
Research Abstract

Plasmids in bacterial cells have the centromere-based segregation system to ensure their faithful transmission to daughter cells, and is being studied as a model system for chromosome partitioning mechanism. We studied the general mechanism underlie these plasmid partitioning machineries, and found that SopA and ParA proteins do not directly pull of push plasmid DNA, but rather guide the plasmids to the right direction by making the gradient of protein concentrations. Notably, the gradient of SopA or ParA proteins were formed from the polymerizing center within the cell (SopA forms a filament within the cell, and ParA locates on the nuclear). We have been investigating transmission mechanism of bacterial chromosome as well. As the chromosome transmission guidance protein, we identified novel factor MigP and a component of the complex I of electron transport chain NuoG. both are membrane associating proteins and we expect DNA transmission mechanism could associates membrane localization.
DNA checkpoint mechanism in eukaryotic cells stops the cell cycle progression when the cell suffers from DNA damage. We identified the mechanism also affects DNA repair
activities themselves besides its regulate cell cycle progression. We further studied the checkpoint mechanism in dimorphic yeast system. There we found that Chk1 could induce hypha
formation in response to DNA damage, and thus we defined it as the novel cell cycle regulatory function of DNA checkpoint.

Report

(6 results)
  • 2009 Annual Research Report   Final Research Report ( PDF )
  • 2008 Annual Research Report
  • 2007 Annual Research Report
  • 2006 Annual Research Report
  • 2005 Annual Research Report
  • Research Products

    (52 results)

All 2010 2009 2008 2007 2006 2005 Other

All Journal Article (32 results) (of which Peer Reviewed: 14 results) Presentation (17 results) Book (2 results) Remarks (1 results)

  • [Journal Article] The DNA damage checkpoint regulates a transition between yeast and hyphal growth in Schizosaccharomyces japonicus.2010

    • Author(s)
      Furuya K, Niki H.
    • Journal Title

      Mol. Cell. Biol. 30

      Pages: 2909-2917

    • Related Report
      2009 Final Research Report
  • [Journal Article] The DNA damage checkpoint regulates a transition between yeast and hyphal growth in Schizosaccharomyices japonicus2010

    • Author(s)
      Furuya, K.
    • Journal Title

      Mol.Cell.Biol. (印刷中)

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A genome-scale proteomic screen identifies a role for DnaK in chaperoning of polar autotransporters in Shigella J.2009

    • Author(s)
      anakiraman, A., Fixen, K.R., Gray, A.N. Niki, H., Goldberg, M. B
    • Journal Title

      Bacteriol. 191

      Pages: 6300-6311

    • Related Report
      2009 Final Research Report
  • [Journal Article] Replication initiator DnaA of Escherichia coli changes its assembly form on the replication origin during the cell cycle.2009

    • Author(s)
      Nozaki S, Niki, H, Ogawa, T
    • Journal Title

      J. Bacteriol 191

      Pages: 4807-4814

    • Related Report
      2009 Final Research Report
  • [Journal Article] Genetic mechanism regulating bacterial cell shape and metabolism2009

    • Author(s)
      Shiomi, D., Mori, H., Niki, H.
    • Journal Title

      Communicative & Integrative Biology 3号2巻

      Pages: 219-220

    • Related Report
      2009 Final Research Report
  • [Journal Article] Isolation of heterothallic haploid and auxotrophic mutants of Schizosaccharomyces japonicus.2009

    • Author(s)
      . Furuya, K., NIKI, H.
    • Journal Title

      Yeast 4号26巻

      Pages: 221-233

    • Related Report
      2009 Final Research Report
  • [Journal Article] A genome-scale proteomic screen identifies a role for DnaK in chaperoning of polar autotransporters in Shigella2009

    • Author(s)
      Janakiraman, A.
    • Journal Title

      J.Bacteriol. 191

      Pages: 6300-6311

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Replication initiator DnaA of Escherichia coli changes its assembly form on the replication origin during the cell cycle2009

    • Author(s)
      Nozaki, S.
    • Journal Title

      J.Bacteriol. 191

      Pages: 4807-4814

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Isolation of heterothallic haploid and auxotroph mutants of Schizosacc haromyces japonicus2009

    • Author(s)
      Furuya, K.
    • Journal Title

      Yeast 26

      Pages: 221-233

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 染色体の分配とM期制御2009

    • Author(s)
      青木敬太
    • Journal Title

      PNE蛋白質核酸酵素 54巻, 4号

      Pages: 406-408

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] バクテリアの染色体配分の普遍性と多様性2009

    • Author(s)
      波田野俊之
    • Journal Title

      PNE蛋白質核酸酵素 54巻, 4号

      Pages: 409-415

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Genetic mechanism regulating bacterial cell shape and metabolism2009

    • Author(s)
      Siomi, D.
    • Journal Title

      Commuicative & Integrative Biology 2

      Pages: 1-2

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Determination of bacterial rod shape by a novel cytoskeletal membrane protein2008

    • Author(s)
      Shiomi, D, Sakai, M., Niki, H.
    • Journal Title

      EMBO J. 23号27巻

      Pages: 3081-3091

    • Related Report
      2009 Final Research Report
  • [Journal Article] A DNA polymeraseαaccessory protein, Mcl1, is required for propagation of centromere structures in fission yeast.2008

    • Author(s)
      Natsume T, Tsutsui Y, Sutani T, Dunleavy E, Pidoux A, Iwasaki H, Shirahige K, Allshire R, Yamao F.
    • Journal Title

      PLoS ONE 3

    • Related Report
      2009 Final Research Report
  • [Journal Article] Fission yeast Swi5 protein, a novel DNA recombination mediator2008

    • Author(s)
      Haruta, N., Akamatsu, Y., Tsutsui, Y., Kurokawa, Y., Murayama, Y., Arcangioli, B., Iwasaki, H.
    • Journal Title

      DNA Repair (Amst) 1号7巻

      Pages: 1-9

    • Related Report
      2009 Final Research Report
  • [Journal Article] Determination of bacterial rod shape by a novel cytoskeletal membraneprotein2008

    • Author(s)
      Siomi, D.
    • Journal Title

      The EMBO Journal 27

      Pages: 3081-3091

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A DNA polymerase a accessory protein, Mcl1, is required for propagation of centromere structures in fission yeast2008

    • Author(s)
      Natsume T.
    • Journal Title

      PLoS ONE (In press)

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Mus81 is essential for sister chromatid recombination at broken replication forks.2008

    • Author(s)
      Roseaulin L.
    • Journal Title

      EMBO J. (In press)

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Oscillating focus of SopA associated with filamentous structure guides partitioning of F plasmid.2007

    • Author(s)
      Hatano, T., Yamaichi, Y., Niki, H.
    • Journal Title

      Mol. Microbiol. 64

      Pages: 1198-11213

    • Related Report
      2009 Final Research Report
  • [Journal Article] Rad3-dependent phosphorylation of the checkpoint clamp regulates repair-pathway choice2007

    • Author(s)
      Kai M., Furuya, K., Paderi F., Carr AM. Wang TS.
    • Journal Title

      Nature Cell Biology 6号9巻

      Pages: 691-697

    • Related Report
      2009 Final Research Report
  • [Journal Article] Escherichia coli with a linear genome2007

    • Author(s)
      Cu, i T., Moro-oka, N., Ohsumi K., Kodama, K., Ohshima, T., Ogasawara, N., Mori, H., Wanner, B., Niki, H., Horiuchi, T.
    • Journal Title

      EMBO Rep 2号8巻

      Pages: 181-187

    • Related Report
      2009 Final Research Report
  • [Journal Article] The trans-envelope Tol-Pal complex is part of the cell division machinery and required for proper outer-membrane invagination during cell constriction in E. coli.2007

    • Author(s)
      Gerding, M.A., Ogata, Y., Pecora, N.D., Niki. H., de Boer, PA.
    • Journal Title

      Mol Microbiol. 4号63巻

      Pages: 1008-1025

    • Related Report
      2009 Final Research Report
  • [Journal Article] Fission Yeast Swi5/Sfr1 and Rhp55/Rhp57 Deferentially Regulate Rhp51-dependent Recombination outcomes2007

    • Author(s)
      Y. Akamatsu, Y. Tsutsui, T. Morishita, MD Shahjahan P Siddique, Y. Kurokawa, M. Ikeguchi, F. Yamao, B. Arcangioli, H. Iwasaki
    • Journal Title

      The EMBO J. 5号26巻

      Pages: 1352-1362

    • Related Report
      2009 Final Research Report
  • [Journal Article] Rad3-dependent phosphorylation of the checkpoint clamp regulates repair-pathway choice.2007

    • Author(s)
      Kai, M.
    • Journal Title

      Nature Cell Biol. 9

      Pages: 691-697

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Oscillating focus of SopA associated with filamentous structure guides partitioning of F plasmid.2007

    • Author(s)
      Hatano, T.
    • Journal Title

      Mol. Microbiol. 64

      Pages: 1198-1213

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The trans-envelope Tol-pal complex is part of the cell division machinery and required for proper outer-membrane invagination during cell constriction in E. coli.2007

    • Author(s)
      Gerding MA.
    • Journal Title

      Mol. Microbiol. 63

      Pages: 1008-1025

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Escherichia coli with a linear genome.2007

    • Author(s)
      Cui T.
    • Journal Title

      EMBO Rep 8

      Pages: 181-187

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The trans-envelope Tol-Pal complex is part of the cell division machinery and required for proper outer-membrane invagination during cell constriction in E. coli.2007

    • Author(s)
      Gerding, M.A., Ogata, Y., Pecora, N.D., Nik, i H., de Boer, PA
    • Journal Title

      Mol Microbiol. 63 ・ 4

      Pages: 1008-1025

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Escherichia coli with a linear genome2007

    • Author(s)
      Cu, i T., Moro-oka, N., Ohsumi, K., Kodama, K., Ohshima, T., Ogasawara, N., Mori, H., Wanner, B., Niki, H., Horiuchi, T.
    • Journal Title

      EMBO Rep. 8 ・ 2

      Pages: 181-187

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Oscillating Focus of SopA Associated with Helical Structure Guides Partitioning of F-plasmid2007

    • Author(s)
      Hatano, T., Ymaichi, Y., Nik, i H.
    • Journal Title

      Mol Microbiol. (In press)

    • Related Report
      2006 Annual Research Report
  • [Journal Article] The Swi5-Sfr1 complex stimulates Rhp51/Rad51- and Dmc1-mediated DNA strand exchange in vitro2006

    • Author(s)
      Haruta, N., Kurokawa, Y., Murayama, Y., Akamatsu, Y., Unzai, S., Tsutsui, Y., Iwasaki, H.
    • Journal Title

      Nature Struct. Mole. Biol. 9号13巻

      Pages: 823-830

    • Related Report
      2009 Final Research Report
  • [Journal Article] Genetic and physical interactions between Schizosaccharomyces pombe Mcl1 and Rad2, Dna2 and DNA polymeraseα: evidence for a multifunctional role of Mcl1 in DNA replication and repair.2005

    • Author(s)
      Tsutsui Y, Morishita T, Natsume T, Yamashita K, Iwasaki H, Yamao F, Shinagawa H.
    • Journal Title

      Curr. Genet. 48

      Pages: 34-43

    • Related Report
      2009 Final Research Report
  • [Presentation] CHK1 DEPENDENT TRANSITION OF YEAST GROWTH TO HYPHAL GROWTH IN SCHIZOSACCHAROMYCES JAPONICUS2009

    • Author(s)
      Niki, H.
    • Organizer
      The 5th International Fission Yeast Meeting, Pombe 2009
    • Place of Presentation
      Tokyo
    • Year and Date
      2009-10-26
    • Related Report
      2009 Final Research Report
  • [Presentation] 桿菌の形を決める新規の細胞骨格性タンパク質2009

    • Author(s)
      塩見大輔
    • Organizer
      細菌学会
    • Place of Presentation
      名古屋
    • Year and Date
      2009-03-13
    • Related Report
      2008 Annual Research Report
  • [Presentation] Contribution of formation of the Ter domain for proper segregation of bacterial nucleoids2009

    • Author(s)
      Niki H.
    • Organizer
      Chromosome Dynamics Gordon Conference
    • Place of Presentation
      Il Ciocco Hotel and Resort Lucca(Barga), Italy
    • Related Report
      2009 Annual Research Report
  • [Presentation] Multistep activation of Genomic Stability pathway2008

    • Author(s)
      古谷寛治
    • Organizer
      分子生物学会
    • Place of Presentation
      神戸
    • Year and Date
      2008-12-12
    • Related Report
      2008 Annual Research Report
  • [Presentation] Multistep Activation of Genomic stability pathway2008

    • Author(s)
      古谷寛治
    • Organizer
      3R 2008 Symposium
    • Place of Presentation
      つま恋
    • Year and Date
      2008-10-27
    • Related Report
      2008 Annual Research Report
  • [Presentation] Multiustep Activation of Genomic stability Pathway2008

    • Author(s)
      古谷寛治
    • Organizer
      Asian-Pacific regional Fission Yeast Meeting
    • Place of Presentation
      Singapore
    • Year and Date
      2008-06-25
    • Related Report
      2008 Annual Research Report
  • [Presentation] Multistep activation og genomic stability patwhay2008

    • Author(s)
      古谷寛治
    • Organizer
      International Chromosome Dynamics Symposium in Ise
    • Place of Presentation
      志摩
    • Year and Date
      2008-05-28
    • Related Report
      2008 Annual Research Report
  • [Presentation] Multistep activation of genomic stability pathway2008

    • Author(s)
      古谷寛治
    • Organizer
      遺伝研国際シンポジウム
    • Place of Presentation
      三島
    • Year and Date
      2008-05-26
    • Related Report
      2008 Annual Research Report
  • [Presentation] 大腸菌の形態形成を司る新規因子の解析2008

    • Author(s)
      塩見大輔
    • Organizer
      21世紀大腸菌研究会
    • Place of Presentation
      藤枝市
    • Related Report
      2008 Annual Research Report
  • [Presentation] Construction and Observation of S. japonicus mutants2008

    • Author(s)
      Aoki, K
    • Organizer
      Asia-Pacific Regional S. pombe Meeting 2008, Singapore
    • Place of Presentation
      シンガポール
    • Related Report
      2008 Annual Research Report
  • [Presentation] Screening new mitotic mutants in S. japonicus2008

    • Author(s)
      Aoki, K
    • Organizer
      3R 2008 Symposium.
    • Place of Presentation
      つま恋
    • Related Report
      2008 Annual Research Report
  • [Presentation] Novel cell shape determinant in Escherichia coli2008

    • Author(s)
      塩見大輔
    • Organizer
      Gordon research Conference "Bacterial Cell Surface"
    • Place of Presentation
      New England, NH, USA
    • Related Report
      2008 Annual Research Report
  • [Presentation] Segregation of nucleolus during mitosis2007

    • Author(s)
      Niki, H.
    • Organizer
      Gordon Research Conference "Chromosome Dynamics"
    • Place of Presentation
      Biddeford, ME
    • Year and Date
      2007-08-12
    • Related Report
      2009 Final Research Report
  • [Presentation] Segregation of nucleolus during mitosis2007

    • Author(s)
      Niki, H.
    • Organizer
      Gordon Research Conference "Chromosome Dynamics"
    • Place of Presentation
      Biddeford, ME. USA
    • Related Report
      2007 Annual Research Report
  • [Presentation] CHROMOSOME AND NUCLEUS DYNAMICS OF THE FISSON YEAST, S. japonicus DURING MITOSIS AND MEOSIS2007

    • Author(s)
      Furuya, K.
    • Organizer
      Fourth International Fission Yeast Meeting
    • Place of Presentation
      Copenhagen. Denmark
    • Related Report
      2007 Annual Research Report
  • [Presentation] GENETIC ANALYSIS IN Schizosaccharomyces japonicus, WHICH IS A NEW MODEL YEAST FOR RESEARCH OF CHROMOSOMA AND NUCLEUS2007

    • Author(s)
      Furuya, K.
    • Organizer
      Fourth International Fission Yeast Meeting
    • Place of Presentation
      Copenhagen. Denmark
    • Related Report
      2007 Annual Research Report
  • [Presentation] Guidance of actively partitioning plasmid by a herical structure of the ATPase, SopA2005

    • Author(s)
      Hatano, T., Niki, H.
    • Organizer
      ICGEB Workshop on chromosome duplication and segregation
    • Place of Presentation
      Trieste, Italy
    • Related Report
      2009 Final Research Report
  • [Book] 分子生物学イラストレイテッド2009

    • Author(s)
      柳原克彦、仁木宏典
    • Publisher
      羊土社
    • Related Report
      2009 Final Research Report
  • [Book] 蛋白質核酸酵素2009

    • Author(s)
      青木敬太、仁木宏典
    • Publisher
      共立出版
    • Related Report
      2009 Final Research Report
  • [Remarks]

    • URL

      http://www.nig.ac.jp/labs/NigPrjct/sensai/index.html

    • Related Report
      2009 Final Research Report

URL: 

Published: 2005-04-01   Modified: 2018-03-28  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi