The network regulating the chromosome cycle

• Project/Area Number: 17080014
• Research Category: Grant-in-Aid for Scientific Research on Priority Areas
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: Tokyo Metropolitan Organization for Medical Research
• Principal Investigator: MASAI Hisao 財団法人東京都医学研究機構, 東京都臨床医学総合研究所, 参事研究員 (40229349)
• Co-Investigator(Kenkyū-buntansha): TURIMOTO Tosiki 九州大学, 大学院・理学研究科, 教授 (30163885) ; NIKI Hironori 国立遺伝子研究所, 系統生物研究センター, 教授 (70208122) ; 升方 久夫 大阪大学, 大学院・理学研究科, 教授 (00199689) ; 篠原 彰 大阪大学, 蛋白質研究所, 教授 (00252578)
• Co-Investigator(Renkei-kenkyūsha): MASUKATA Hisao 大阪大学, 大学院・理学研究科, 教授 (00199689) ; SINOHARA Akira 大阪大学, 蛋白質研究所, 教授 (00252578)
• Project Period (FY): 2005 – 2009
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥50,500,000 (Direct Cost: ¥50,500,000); Fiscal Year 2010: ¥3,000,000 (Direct Cost: ¥3,000,000); Fiscal Year 2009: ¥9,500,000 (Direct Cost: ¥9,500,000); Fiscal Year 2008: ¥9,500,000 (Direct Cost: ¥9,500,000); Fiscal Year 2007: ¥9,500,000 (Direct Cost: ¥9,500,000); Fiscal Year 2006: ¥9,500,000 (Direct Cost: ¥9,500,000); Fiscal Year 2005: ¥9,500,000 (Direct Cost: ¥9,500,000)
• Keywords: Cdc7キナーゼ / DNA複製 / 染色体ダイナミクス / Claspin/Mrc1 / リン酸化 / 細胞死誘導 / がん細胞 / 複製フォーク / DNA / クロマチン構造 / 染色体サイクル / 染色体分配 / 染色体複製 / 組換え / 複製装置

Research Abstract

細胞の増殖や分化の過程の大部分は染色体機能により制御される。また、染色体の動態の変動は、種々の疾患あるいは、生物種の進化とも密接に関連する。したがって、染色体の忠実な複製と安定な維持、あるいは、組換えや転移による変動の分子機構を理解することは、細胞の増殖、分化の基本機構を理解するために必須であるのみでなく、疾患の原因や、個体や種の多様性獲得の分子基盤を理解する上でも必須である。本申請研究では、染色体の動態を制御する分子機構について、複製、組換え、分配などが共役して統合的に制御されるメカニズムに焦点をあて、染色体機能制御機構の全体像を明らかにすることが目的である。
具体的には、染色体サイクルと染色体ネットワークという2つのキーワードのもとに以下の4つの研究項目について研究をすすめた。(1)染色体の複製(2)染色体の恒常性維持と変動(3)染色体の分配(4)染色体ネットワーク。平成22年には、終了シンポジウムを開催し、成果を発表するとともに、最終的な成果報告書をまとめ24年1月に提出した。
本特定領域研究では複製因子、組換え因子などのゲノムワイドでの相互作用の細胞周期制御、新規複製制御因子あるいは組換え因子の同定、複製フォークにおけるタンパク質-タンパク質相互作用、リン酸化による機能制御機構、組換えの基本的分子機構の解明、Cdc7の組換え開始における新規標的の解明など多くの新しい知見がもたらされた。最終評価においてもA+(最高レベル)の評価を得ることができた

Research Products

• [Journal Article] Cdc7 (2012)
  • Author(s): Masai, H.
  • Journal Title: The Encyclopedia of Signaling Molecules Springer Reference and Database Publishing Volume: (印刷中)
  • Peer Reviewed
• [Journal Article] Dbf4 (2012)
  • Author(s): Masai, H.
  • Journal Title: The Encyclopedia of Signaling Molecules Springer Reference and Database Publishing Volume: (印刷中)
  • Peer Reviewed
• [Journal Article] Rif1 is a global regulator of timing of replication origin firing in fission yeast (2012)
  • Author(s): Hayano, M., Kanoh, Y., Matsumoto, S., Shrahige, K., Masai, H.
  • Journal Title: Genes and Development Volume: 26 Pages: 137-150
  • Peer Reviewed
• [Journal Article] c-Jun N-terminal Kinase (JNK)-Mediated Rad18 Phosphorylation Facilitates Polη Recruitment to Stalled Replication Forks (2012)
  • Author(s): Barkley, L.R., Palle, K., Durando, M., Day, T.A., Gurkar, A., Kakusho, N., Li, J., Masai, H., Vaziri, C.
  • Journal Title: Mol.Biol.Cell. Volume: (Epub ahead of print)
  • Peer Reviewed
• [Journal Article] EBNA1-dependent recruitment of Orc on OriP of Epstein-Barr virus with purified proteins : Stimulation by Cdc6 through Its direct interaction with EBNA1 (2012)
  • Author(s): Moriyama, K., Yoshizawa-Sugata, N., Obuse, C., Tsurimoto, T., Masai, H.
  • Journal Title: J.Biol.Chem. Volume: (印刷中)
  • Peer Reviewed
• [Journal Article] Mechanism of cancer cell death induced by depletion of an essential replication regulator (2012)
  • Author(s): Ito, S., Ishii, A., Kakusho, N., Taniyama, C., Yamazaki, S., Sakaue-Sawano, A., Miyawaki, A., Masai, H.
  • Journal Title: PLos One Volume: (印刷中)
  • Peer Reviewed
• [Journal Article] Purification of replication factors using insect and mammalian cell expression systems (2012)
  • Author(s): Uno, S., You, Z., Masai, H.
  • Journal Title: Methods Volume: (印刷中)
  • Peer Reviewed
• [Journal Article] Regulation of DNA replication timing on human chromosome by a cell-type specific DNA binding protein SATB1 (2012)
  • Author(s): Oda, M., Kanoh, Y., Watanabe, Y., Masai, H.
  • Journal Title: PLos One Volume: (印刷中)
  • Peer Reviewed
• [Journal Article] Molecular mechanism of activation of human Cdc7 kinase : Bipartite interaction with Dbf4/ASK stimulates ATP binding and substrate recognition (2011)
  • Author(s): Kitamura, R., Fukatsu, R., Kakusho, N., Cho, Y-S., Taniyama, C., Yamazaki, S., Toh, G-T., Yanagi, K., Arai, N., Chang, H-J., Masai, H.
  • Journal Title: J.Biol.Chem. Volume: 286 Pages: 23031-23043
  • Peer Reviewed
• [Journal Article] Pre-firing binding of Mrc1 defines the early-firing origins which are selectively hyper-activated upon loss of fork stabilizing factors in fission yeast (2011)
  • Author(s): Hayano, M., Kanoh, Y., Matsumoto, S., Kakusho, N., Masai, H.
  • Journal Title: Mol.Cell.Biol. Volume: 31 Pages: 2380-2389
  • Peer Reviewed
• [Journal Article] Efficient expression and purification of human replication for k-stabilizing factor, Claspin, from mammalian cells : DNA binding activity and novel protein interactions (2011)
  • Author(s): Uno, S, Masai, H
  • Journal Title: Genes to Cells. Volume: 16 Pages: 842-856
  • Peer Reviewed
• [Journal Article] Multiple pathways can bypass the essential role of fission yeast Hsk1 kinase in DNA replication initiation (2011)
  • Author(s): Matsumoto, S., Hayano, M., Kanoh. Y., Masai, H.
  • Journal Title: J.Cell Biol. Volume: 195 Pages: 387-401
  • Peer Reviewed
• [Journal Article] RecQL4 : a helicase linking formation and maintenance of a replication fork (2011)
  • Author(s): Masai, H.
  • Journal Title: J.Biochem. Volume: 149 Pages: 629-631
  • NAID: 10030585512
  • Peer Reviewed
• [Journal Article] Deregulated Cdc6 inhibits DNA replication and suppresses Cdc7-mediated phosphorylation of Mcm2-7 complex. (2010)
  • Author(s): Kundu, L.R., Kumata, Y, Kakusho, N., Watanabe, S., Furukohri, A., Waga, S., Sekia, M., Masai, H., Enomoto, T., Tada, S.
  • Journal Title: Nucleic Acid Res. PMID(in press [Epub ahead of print]) Pages: 20421204-20421204
  • Peer Reviewed
• [Journal Article] Casein Kinase 2-dependent phosphorylation of human Rad9 mediates the interaction between human Rad9-Hus1-Rad1 complex and TopBP1. (2010)
  • Author(s): Takeishi, Y., Ohashi, E., Ogawa, K., Masai, H., Obuse, C., Tsurimoto, T.
  • Journal Title: Genes to Cells (in press)
  • NAID: 120005227474
  • Peer Reviewed
• [Journal Article] Eukaryotic DNA replication; where, when and how? (2010)
  • Author(s): Masai, H., Matsumoto, S., You, Z., Yoshizawa-Sugata, N., Oda, M.
  • Journal Title: Annual Rev. Biochem. PMID(in press. [Epub ahead of print]) Pages: 20373915-20373915
  • Peer Reviewed
• [Journal Article] The DNA damage checkpoint regulates a transition between yeast and hyphal growth in Schizosaccharomyces japonicus. (2010)
  • Author(s): Furuya K, Niki H.
  • Journal Title: Mol. Cell. Biol. (in press)
  • Peer Reviewed
• [Journal Article] Casein Kinase 2-dependent phosphorylation of human Rad9 mediates the interaction between human Rad9-Hus1-Rad1 complex and TopBP1. (2010)
  • Author(s): Takeishi Y, Ohashi E, Ogawa K, Masai H, Obuse C, Tsurimoto T.
  • Journal Title: Genes Cells (in press)
  • NAID: 120005227474
  • Peer Reviewed
• [Journal Article] Eukaryotic DNA replication ; where, when and how? (2010)
  • Author(s): Masai, H., Matsumoto, S., You, Z., Sugata-Yoshizawa, N., Oda, M.
  • Journal Title: Annual.Rev.Biochem. (In press)
  • Peer Reviewed
• [Journal Article] Selection of replication origins in fission yeast. (2010)
  • Author(s): Matsumoto, S., Hayano, M., Kanoh,Y., Masai, H.
  • Journal Title: Chromosoma (In press)
  • Peer Reviewed
• [Journal Article] Cdc7L1. (2010)
  • Author(s): Toh G-T., Masai, H.
  • Journal Title: UCSD-Nature Molecule Pages (In press)
  • Peer Reviewed
• [Journal Article] Stalled replication forks : Making ends meet for recognition and stabilization. (2010)
  • Author(s): Masai, H., Tanaka, T., Kohda, D.
  • Journal Title: BioEssays (In press)
  • Peer Reviewed
• [Journal Article] Bacterial primosome. (2010)
  • Author(s): Tanaka, T., Masai, H.
  • Journal Title: Encyclopedia of Life Sciences (In press)
  • Peer Reviewed
• [Journal Article] Bacterial replication fork. (2010)
  • Author(s): Tanaka, T., Masai, H.
  • Journal Title: Encyclopedia of Life Sciences (In press)
  • Peer Reviewed
• [Journal Article] The DNA damage checkpoint regulates a transition between yeast and hyphal growth in Schizosaccharomyces japonicus. (2010)
  • Author(s): Furuya K, Niki H.
  • Journal Title: Mol Cell Biol. (In press)
  • Peer Reviewed
• [Journal Article] Interactions between Swi1-Swi3, Mrc1 and S phase kinase, Hsk1 may regulate cellular responses to stalled replication forks in fission yeast. (2009)
  • Author(s): Shimmoto, S., Matsumoto, S., Hayano, M., Yokoyama, M., Noguchi, E., Russell, P., Masai, H.
  • Journal Title: Genes to Cells 14 Pages: 669-682
  • Peer Reviewed
• [Journal Article] Replisome progression complex links DNA replication to sister chromatid cohesion in Xenopus egg extracts. (2009)
  • Author(s): Tanaka, H., Kubota, Y., Tsujimura, T., Kumano, M., Masai, H., Takisawa, H.
  • Journal Title: Genes to Cells 14 Pages: 949-63
  • Peer Reviewed
• [Journal Article] Roles of human AND-1 in chromosome transactions in S phase. (2009)
  • Author(s): Yoshizawa-Sugata, N., Masai, H.
  • Journal Title: J. Biol. Chem. 284 Pages: 20718-20728
  • Peer Reviewed
• [Journal Article] Isolation of heterothallic haploid and auxotroph mutants of Schizosaccharomyces japonicus. (2009)
  • Author(s): Furuya K, Niki H.
  • Journal Title: Yeast. 26 Pages: 221-233
  • Peer Reviewed
• [Journal Article] Replication initiator DnaA of Escherichia coli changes its assembly form on the replication origin during the cell cycle. (2009)
  • Author(s): Nozaki S, Niki, H, Ogawa, T.
  • Journal Title: J. Bacteriol. 191 Pages: 4807-4814
  • Peer Reviewed
• [Journal Article] A genome-scale proteomic screen identifies a role for DnaK in chaperoning of polar autotransporters in Shigella J. (2009)
  • Author(s): Janakiraman, A., Fixen, K.R., Gray, A.N. Niki, H., Goldberg, M.B.
  • Journal Title: Bacteriol. 191 Pages: 6300-6311
  • Peer Reviewed
• [Journal Article] Genetic mechanism regulating bacterial cell shape and metabolism (2009)
  • Author(s): Shiomi D, Mori, H, Niki, H.
  • Journal Title: Commu. & Integ. Biol. 2 Pages: 219-220
  • Peer Reviewed
• [Journal Article] Physical and functional interaction between WRNIP1 and RAD18. (2009)
  • Author(s): Yoshimura A, Seki M, Kanamori M, Takeishi S, Tsurimoto T, Tada S, Enomoto T.
  • Journal Title: Genes Genet. Syst. 84 Pages: 171-178
  • NAID: 10024791852
  • Peer Reviewed
• [Journal Article] Interactions between Swil-Swi3, Mrc1 and S phase kinase, Hsk1 mayregulate cellular responses to stalled replication forks in fission yeast. (2009)
  • Author(s): Shimmoto, S., Matsumoto, S., Hayano, M., Yokoyama, M., Noguchi, E., Russell, P., Masai, H.
  • Journal Title: Genes to Cells 14 Pages: 669-682
  • Peer Reviewed
• [Journal Article] Roles of human AND-1 in chromosome transactions in S phase. (2009)
  • Author(s): Yoshizawa-Sugata, N., Masai, H.
  • Journal Title: J.Biol.Chem. 284 Pages: 20718-20728
  • Peer Reviewed
• [Journal Article] Deregulated Cdc6 inhibits DNA replication and suppresses Cdc7-mediated phosphorylation of Mcm2-7 complex. (2009)
  • Author(s): Kundu, L.R., Kumata, Y., Kakusho, N., Watanabe, S., Furukohri, A., Waga, S., Sekia, M., Masai, H., Enomoto, T., Tada, S.
  • Journal Title: Nucleic Acid Res. (In press)
  • Peer Reviewed
• [Journal Article] Casein Kinase 2-dependent phosphorylation of human Rad9 mediates the interaction between human Rad9-Hus1-Rad1 complex and TopBP1. (2009)
  • Author(s): Takeishi, Y., Ohashi, E., Ogawa, K., Masai, H., Obuse, C., Tsurimoto, T.
  • Journal Title: Genes to Cells (In press)
  • NAID: 120005227474
  • Peer Reviewed
• [Journal Article] Drug Design with Cdc7 kinase, a potential novel cancer therapy target. (2009)
  • Author(s): Sawa, M., Masai, H.
  • Journal Title: Drug Design, Development and Therapy 2 Pages: 255-264
  • Peer Reviewed
• [Journal Article] ASK/Dbf4 (2009)
  • Author(s): Toh, G.K., Masai, H.
  • Journal Title: UCSD-Nature Molecule Pages
  • Peer Reviewed
• [Journal Article] The heterochromatin protein Swi6/HP1 activates replication origins at the pericentromeric region and silent mating-type locus. (2009)
  • Author(s): Hayashi MT, Takahashi TS, Nakagawa T, Nakayama J, Masukata H.
  • Journal Title: Nat Cell Biol. 11 Pages: 357-362
  • Peer Reviewed
• [Journal Article] Function of multiple clamp and loader systems in eukaryotic chromosome replication (2009)
  • Author(s): Shiomi Y, Tsurimoto T.
  • Journal Title: Seikagaku 81 Pages: 386-389
  • NAID: 10024916697
  • Peer Reviewed
• [Journal Article] Isolation of heterothallic haploid and auxotrophic mutants of Schizosaccharomyces japonicus. (2009)
  • Author(s): Furuya K, Niki H.
  • Journal Title: Yeast. 26 Pages: 221-233
  • Peer Reviewed
• [Journal Article] Interations between Swi1-Swi3, Mrc1 and S phase kinase, Hsk1 may regulate cellular responses to stalled replication forks in fission yeast. (2009)
  • Author(s): Shimmoto, M., Matsumoto, S., Odagiri, Y., Noguchi, E., Russell, P. and Masai, H.
  • Journal Title: Genes to Cells (印刷中)
  • Peer Reviewed
• [Journal Article] ASK (2009)
  • Author(s): Toh, G.K., and Masai, H.
  • Journal Title: UCSD-Nature Molecule Pages (印刷中)
  • Peer Reviewed
• [Journal Article] The heterochromatin protein Swi6/HP1 activates replication origins at the pericentromeric region and silent mating-type locus. (2009)
  • Author(s): Hayashi, MT., Takahashi, TS., Nakagawa, T., Nakayama, J., Masukata, H.
  • Journal Title: Nat Cell Biol. Pages: 357-62
  • Peer Reviewed
• [Journal Article] Cdc7 kinase is required for Claspin phosphorylation in DNA replication checkpoint. (2008)
  • Author(s): Kim, J-M., Kakusho, N., Yamada, M., Kanoh, Y., Takemoto, N., Masai, H.
  • Journal Title: Oncogene 27 Pages: 3475-3482
  • Peer Reviewed
• [Journal Article] Cdc7-dependent phosphorylation of Mer2 facilitates initiation of yeast meiotic recombination. (2008)
  • Author(s): Sasanuma, H., Hirota, K., Fukuda, T., Kakusho, N., Kugou, N., Kawasaki, Y., Shibata, T., Masai, H., Ohta, K.
  • Journal Title: Genes & Dev. 22 Pages: 398-410
  • Peer Reviewed
• [Journal Article] Spatio-temporal dynamics of multicellular cell cycle progression. (2008)
  • Author(s): Sakaue-Sawano, A., Kurokawa, H., Morimura, T., Hanyu, A., Hama, H., Kashiwagi, S., Fukami, K., Imamura, T., Ogawa, M., Masai, H., Miyawaki, A.
  • Journal Title: Cell 132 Pages: 487-498
  • Peer Reviewed
• [Journal Article] Identification of stimulators and inhibitors of CDC7 kinase in vitro. (2008)
  • Author(s): Kakusho, N., Taniyama, C., Masai, H.
  • Journal Title: J. Biol. Chem. 283 Pages: 19211-19218
  • Peer Reviewed
• [Journal Article] Cdt1 forms a complex with MCM and activates its helicase activity. (2008)
  • Author(s): You, Z., Masai, H.
  • Journal Title: J. Biol. Chem. 283 Pages: 24469-24477
  • Peer Reviewed
• [Journal Article] Determination of bacterial rod shape by a novel cytoskeletal membrane protein. (2008)
  • Author(s): Shiomi D, Sakai M, Niki H.
  • Journal Title: EMBO. J 26 Pages: 3081-3091
  • Peer Reviewed
• [Journal Article] CDK inhibitor p21 is degraded by a PCNA coupled Cul4-DDB1Cdt2 pathway during S phase and after UV irradiation. (2008)
  • Author(s): Nishitani H, Shiomi Y, Iida H, Michishita M, Takami T Tsurimoto, T.
  • Journal Title: J Biol Chem. 283 Pages: 29045-29052
  • Peer Reviewed
• [Journal Article] DNA damage induced ubiquitylation of RFC2 subunit of RFC complex. (2008)
  • Author(s): Tomida J, Masuda Y, Hiroaki H, Ishikawa T, Song I, Tsurimoto T, Tateishi S, Shiomi T, Kamei Y, Kim J, Kamiya K, Vaziri C, Ohmori H, Todo T.
  • Journal Title: J Biol Chem. 283 Pages: 9071-9079
  • Peer Reviewed
• [Journal Article] Recognition of forked and single-stranded DNA structures by human RAD18 complexed with RAD6B protein triggers its recruitment to stalled replication forks. (2008)
  • Author(s): Tsuji Y, Watanabe K, Araki K, Shinohara M, Yamagata Y, Tsurimoto T, Hanaoka F, Yamamura K, Yamaizumi M, Tateishi, S.
  • Journal Title: Genes Cells 13 Pages: 343-354
  • Peer Reviewed
• [Journal Article] Crossover assurance and crossover interference are distinctly regulated by the ZMM proteins during yeast meiosis. (2008)
  • Author(s): Shinohara, M., Oh, SD., Hunter, N., Shinohara, A.
  • Journal Title: Nat Genet. Pages: 299-309
  • Peer Reviewed
• [Journal Article] Csm4-dependent telomere movement on nuclear envelope promotes meiotic recombination. (2008)
  • Author(s): Kosaka, H., Shinohara, M., Shinohara, A.
  • Journal Title: PLoS Genet. (オンライン)
  • Peer Reviewed
• [Journal Article] Determination of bacterial rod shape by a novel cytoskeletal membrane protein. (2008)
  • Author(s): Shiomi, D., Sakai, M., Niki, H.
  • Journal Title: EMBO J. Pages: 3081-91
  • Peer Reviewed
• [Journal Article] CDK inhibitor p21 is degraded by a proliferating cell nuclear antigen-coupled Cul4-DDB1Cdt2 pathway during S phase and after UV irradiation. (2008)
  • Author(s): Nishitani, H., Shiomi, Y., Iida, H., Michishita, M., Takami, T., Tsurimoto, T.
  • Journal Title: J Biol Chem. 283 Pages: 29045-52
  • Peer Reviewed
• [Journal Article] Cdt1 forms a complex with MCM and activates its helicase activity. (2008)
  • Author(s): You, Z., and Masai, H.
  • Journal Title: J. Biol. Chem. 283 Pages: 24469-24477
  • Peer Reviewed
• [Journal Article] Cdc7 as a potential new target for cancer therapy. (2008)
  • Author(s): Ito, S., Taniyama, C., Arai, N. and Masai, H.
  • Journal Title: Drug News and Perspectives 21 Pages: 481-488
  • Peer Reviewed
• [Journal Article] Drug Design with Cdc7 kinase, a potential novel cancer therapy target. (2008)
  • Author(s): Sawa, M., and Masai, H.
  • Journal Title: Drug Design, Development and Therapy 2 Pages: 255-264
  • Peer Reviewed
• [Journal Article] Identification of stimulators and inhibitors of CDC7 kinase in vitro. (2008)
  • Author(s): Kakusho, N., Taniyama, C., and Masai, H.
  • Journal Title: J. Biol. Chem. 282 Pages: 19211-19218
  • Peer Reviewed
• [Journal Article] Spatio-temporal dynamics of multicellular cell cycle progression. (2008)
  • Author(s): Sakaue-Sawano, A., Kurokawa, H., Morimura, T., Hanyu, A., Hama, H., Kashiwagi, S., Fukami, K., Imamura, T., Ogawa, M., Masai, H. and Miyawaki, A.
  • Journal Title: Cell 132 Pages: 487-498
  • Peer Reviewed
• [Journal Article] Cdc7-dependent phosphorylation of Mer2 facilitates initiation of yeast meiotic recombination. (2008)
  • Author(s): Sasanuma, H., Hirota, K., Fukuda, T., Kakusho, N., Kugou, N., Kawasaki, Y., Shibata, T., Masai, H. and Ohta, K.
  • Journal Title: Genes Dev. 22 Pages: 398-410
  • Peer Reviewed
• [Journal Article] Cdc7 kinase is required for Claspin phosphorylation in DNA replication checkpoint. (2008)
  • Author(s): Kim, J-M., Yamada, M., Kakusho, N., Kanoh, Y., Takemoto, N., and Masai. H.
  • Journal Title: Oncogene 27 Pages: 3475-3482
  • Peer Reviewed
• [Journal Article] ldentification of stimulators and inhibitors of CDC7 kinase in vitro (2008)
  • Author(s): Kakusho, N., Taniyama, C., and Masai, H.
  • Journal Title: J. Biol. Chem 282(印刷中)
  • Peer Reviewed
• [Journal Article] Spatio-temporal dynamics of multicellular cell cycle progression (2008)
  • Author(s): Sakaue-Sawano, A., Kurokawa, H., Morimura, T., Hanyu, A., Hama, H., Kashiwagi, S., Fukami, K., Imamura, T., Ogawa, M., Masai, H. and Miyawaki, A.
  • Journal Title: Cell 132 Pages: 487-498
  • Peer Reviewed
• [Journal Article] Cdc7-dependent phosphorylation of Mer2 facilitates initiation of yeastmeiotic recombination (2008)
  • Author(s): Sasanuma, H., Hirota, K., Fukuda, T., Kakusho, N., Kugou, N., Kawasaki, Y., Shibata, T., Masai, H. and Ohta, K.
  • Journal Title: Genes Dev 22 Pages: 398-410
  • Peer Reviewed
• [Journal Article] Cdc7 kinase is required for Claspin phosphorylation in DNA replication checkpoint (2008)
  • Author(s): Kim, J-M., Yamada, M., Kakusho, N., Kanoh, Y., Takemoto, N., and Masai, H.
  • Journal Title: Oncogene 27(印刷中)
  • Peer Reviewed
• [Journal Article] Human Tim/Timeless-interacting protein, Tipin, is required for efficient progression of S phase and DNA replication checkpoint. (2007)
  • Author(s): Yoshizawa-Sugata, N., Masai, H.
  • Journal Title: J. Biol. Chem. 282 Pages: 2729-2740
  • Peer Reviewed
• [Journal Article] Escherichia coli PriA protein: Two modes of DNA binding and activation of ATP hydrolysis. (2007)
  • Author(s): Tanaka, T., Mizukoshi, T., Sasaki, K., Kohda, D., Masai, H.
  • Journal Title: J. Biol. Chem. 282 Pages: 19917-19927
  • Peer Reviewed
• [Journal Article] Structural basis of the 3'-end recognition of a leding strand in stalled DNA replication forks by PriA. (2007)
  • Author(s): Sasaki, K., Ose, T., Okamoto, N., Maenaka, K., Tanaka, T., Masai, H., Saito, M., Shirai, T., D. Kohda
  • Journal Title: EMBO J. 26 Pages: 19917-19927
  • Peer Reviewed
• [Journal Article] The trans-envelope Tol-Pal complex is part of the cell division machinery and required for proper outer-membrane invagination during cell constriction in E. (2007)
  • Author(s): Gerding MA, Ogata Y, Pecora ND, Niki H, de Boer PA.
  • Journal Title: coli. Mol. Microbiol. 63 Pages: 1008-1025
  • Peer Reviewed
• [Journal Article] Escherichia coli with a linear genome. (2007)
  • Author(s): Cui T, Moro-oka N, Ohsumi K, Kodama K, Ohshima T, Ogasawara N, Mori H, Wanner B, Niki H, Horiuchi T.
  • Journal Title: EMBO Rep. 8 Pages: 181-187
  • Peer Reviewed
• [Journal Article] Oscillating focus of SopA associated with filamentous structure guides partitioning of F plasmid. (2007)
  • Author(s): Hatano, T., Yamaichi, Y., Niki, H.
  • Journal Title: Mol. Microbiol. 64 Pages: 1198-11213
  • Peer Reviewed
• [Journal Article] A second PCNA loader complex, Ctf18-RFC, stimulates DNA polymerase eta activity. (2007)
  • Author(s): Shiomi Y, Masutani C, Hanaoka F, Kimura H, Tsurimoto, T.
  • Journal Title: J Biol Chem. 282 Pages: 20906-20914
  • Peer Reviewed
• [Journal Article] Dynamics of human replication factors in the elongation phase of DNA replication. (2007)
  • Author(s): Masuda Y, Suzuki M, Piao J, Gu Y, Tsurimoto T, Kamiya K.
  • Journal Title: Nucleic Acids Res. 35 Pages: 6904-6916
  • Peer Reviewed
• [Journal Article] Structural basis of the 3'-end recognition of a leading strand in stalled DNA replication forks by PriA (2007)
  • Author(s): Sasaki, K., Ose, T., Okamoto, N., Maenaka, K., Tanaka, T., Masai, H., Saito, M., Shirai, T. and Kohda, D.
  • Journal Title: EMBO J 26 Pages: 2584-2593
  • Peer Reviewed
• [Journal Article] Human Tim/Timeless-interacting protein, Tipirn, is requlred for efficient progression of S phase and DNA replication checkpoint (2007)
  • Author(s): Yoshizawa-Sugata, N. and Masai, H.
  • Journal Title: J.Biol.Chem. 282 Pages: 2729-2740
  • Peer Reviewed
• [Journal Article] Escherichia coli PriA protein: Two modes of DNA binding and activation of ATP hydrolysis (2007)
  • Author(s): Tanaka, T., Mizukoshi, T., Sasaki, K., Kohda, D. and Masai, H.
  • Journal Title: J.Biol.Chem. 282 Pages: 19917-19927
  • Peer Reviewed
• [Journal Article] Structural basis of the 3'-end recognition of a leading strand in stalled DNA replication forks by PriA. (2007)
  • Author(s): Sasaki, K.
  • Journal Title: EMBO J. (印刷中)
• [Journal Article] Escherichia coli PriA protein : Two modes of DNA binding and activation of ATP hydrolysis. (2007)
  • Author(s): Tanaka, T.
  • Journal Title: J. Biol. Chem. (印刷中)
• [Journal Article] Human Tim/Timeless-interacting protein, Tipin, is required for efficient progression of S phase and DNA replication checkpoint. (2007)
  • Author(s): Yoshizawa-Sugata, N.
  • Journal Title: J. Biol. Chem. 282 Pages: 2729-2740
• [Journal Article] Rad3-Cds1 mediates coupling of initiation of meiotic recombination with DNA replication: Mei4-dependent transcription as a potential target of meiotic checkpoint. (2006)
  • Author(s): Ogino, K., Masai, H.
  • Journal Title: J. Biol. Chem. 281 Pages: 1338-1344
  • Peer Reviewed
• [Journal Article] Stabilization of a stalled replication fork by concerted actions of two helicases. (2006)
  • Author(s): Tanaka, T., Masai, H.
  • Journal Title: J. Biol. Chem. 281 Pages: 3484-3493
  • Peer Reviewed
• [Journal Article] Crystallization and preliminary crystallographic analysis of the N-terminal domain of PriA from Escherichia coli. (2006)
  • Author(s): Sasaki, K., Ose, T., Tanaka, T., Mizukoshi, T., Ishigaki, T., Maenaka, K., Masai, H., Kohda, D.
  • Journal Title: Biochim. Biophys. Acta. 1764 Pages: 157-160
  • Peer Reviewed
• [Journal Article] Nuclear import of Epstein-Barr Virus Nuclear Antigen 1 mediated by NPI-1 - and down-regulated by phosphorylation of the nuclear localization signal for which Lys379 and Arg380 are essential. (2006)
  • Author(s): Kitamura, R., Sekimoto, T., Ito, S., Harada, S., Yamagata, H., Masai, H., Yoneda, H., Yanagi, K.
  • Journal Title: J. Virol. 80 Pages: 979-1991
  • Peer Reviewed
• [Journal Article] Hsk1 kinase is required for induction of meiotic double-stranded DNA breaks without involving checkpoint kinases in fission yeast. (2006)
  • Author(s): Ogino, K., Hirota, K., Matsumoto, S., Takeda, T., Ohta, K., Arai, K., Masai, H.
  • Journal Title: Proc. Natl. Acad. Sci. USA 103 Pages: 8131-8136
  • Peer Reviewed
• [Journal Article] "Replication initiation from a novel origin identified in the Th2 cytokine cluster locus requires a distant conserved non-coding sequence. " (*cocommunicating authors) (2006)
  • Author(s): Hayashida, T., Oda, M., Ohsawa, K., Yamaguchi, A., Giacca, M., Locksley, R.M., Masai H., Miyatake, S. *
  • Journal Title: J. Immunol. 176 Pages: 5446-5454
  • Peer Reviewed
• [Journal Article] Phosphorylation of MCM4 by Cdc7 kinase facilitates its interaction with Cdc45 on the chromatin. (2006)
  • Author(s): Masai, H., Taniyama, C., Ogino, K., Matsui, E., Kakusho, N., Matsumoto, M., Kim, J-M., Ishii, A., Tanaka, T., Kobayashi, T., Tamai, K., Ohtani, K., Arai, K.
  • Journal Title: J. Biol. Chem. 281 Pages: 39249-32961
  • Peer Reviewed
• [Journal Article] Two E3 ubiquitin ligases, SCF-Skp2 and DDB1-Cul4, target human Cdt1 for proteolysis. (2006)
  • Author(s): Nishitani H, Sugimoto N, Roukos V, Nakanishi Y, Saijo M, Obuse C, Tsurimoto T, Nakayama K-I, Nakayama K, Fujita M, Lygerou Z, Nishimoto T.
  • Journal Title: EMBO J. 25 Pages: 1126-1136
  • Peer Reviewed
• [Journal Article] proteins in Genomic Stability. (in DNA Replication and Human Disease ed. DePamphilis M L, Cold Spring Harbor Press NY) (2006)
  • Author(s): Tsurimoto T.
  • Journal Title: PCNA and RFC Pages: 411-434
  • Peer Reviewed
• [Journal Article] n Proliferating Cell Nuclear Antigen (PCNA) ed. Lee H. Research Signpost, Kerala, India (2006)
  • Author(s): Tsurimoto T.
  • Journal Title: PCNA-interacting proteins. Pages: 25-49
  • Peer Reviewed
• [Journal Article] Phosphorylation of MCM4 by Cdc7 kinase facilitates its interaction with Cdc45 on the chromatin. (2006)
  • Author(s): Masai, H.
  • Journal Title: J. Biol. Chem. 281 Pages: 39249-32961
• [Journal Article] Replication initiation from a novel origin identified in the Th2 cytokine cluster locus requires a distant conserved non-coding sequence. (*cocommunicating authors) (2006)
  • Author(s): Hayashida, T.
  • Journal Title: J. Immunol. 176 Pages: 5446-5454
• [Journal Article] Rad3-Cds1 mediates coupling of initiation of meiotic recombination with DNA replication : Mei4-dependent transcription as a potential target of meiotic checkpoint. (2006)
  • Author(s): Ogino, K.
  • Journal Title: J. Biol. Chem. 281 Pages: 1338-1344
• [Journal Article] Stabilization of a stalled replication fork by concerted actions of two helicases. (2006)
  • Author(s): Tanaka, T.
  • Journal Title: J. Biol. Chem. 281 Pages: 3484-3493
• [Journal Article] Crystallization and preliminary crystallographic analysis of the N-terminal domain of PriA from Escherichia coli. (2006)
  • Author(s): Sasaki, K.
  • Journal Title: Biochim. Biophys. Acta. 1764 Pages: 157-160
• [Journal Article] Nuclear import of Epstein-Barr Virus Nuclear Antigen 1 mediated by NiPI-1 (Importin α5) is up- and down-regulated by phosphorylation of the nuclear localization signal for which Lys379 and Arg380 are essential. (2006)
  • Author(s): Kitamura, R.
  • Journal Title: J. Virol. 80 Pages: 1979-1991
• [Journal Article] Hsk1 kinase is required for induction of meiotic double-stranded DNA breaks without involving checkpoint kinases in fission yeast. (2006)
  • Author(s): Ogino, K., Hirota, K., Matsumoto, S., Takeda, T., Ohta, K., Arai, K., Masai, H.
  • Journal Title: Proc.Natl.Acad.Sci.USA (印刷中)(accepted for publication)
• [Journal Article] "Replication initiation from a novel origin identified in the Th2 cytokine cluster locus requires a distant conserved non-coding sequence." (*cocommunicating authors) (2006)
  • Author(s): Hayashida, T., Oda, M., Ohsawa, K., Yamaguchi A., Giacca, M., Locksley, R.M., Masai H., Miyatake, S.
  • Journal Title: J.Immunol. (印刷中)
• [Journal Article] Rad3-Cds1 mediates coupling of initiation of meiotic recombination with DNA replication : Mei4-dependent transcription as a potential target of meiotic checkpoint. (2006)
  • Author(s): Ogino, K., Masai, H.
  • Journal Title: J.Biol.Chem. 281 Pages: 1338-1344
• [Journal Article] Stabilization of a stalled replication fork by concerted actions of two helicases. (2006)
  • Author(s): Tanaka, T., Masai, H.
  • Journal Title: J.Biol.Chem. 281 Pages: 3484-3493
• [Journal Article] Crystallization and preliminary crystallographic analysis of the N-terminal domain of PriA from Escherichia coli. (2006)
  • Author(s): Sasaki, K., Ose, T., Tanaka, T., Mizukoshi, T., Ishigaki, T., Maenaka, K., Masai, H., Kohda, D.
  • Journal Title: Biochim.Biophys.Acta. 1764 Pages: 157-160
• [Journal Article] Nuclear import of Epstein-Barr Virus Nuclear Antigen 1 mediated by NPI-1 (Importin ・5) is up- and down-regulated by phosphorylation of the nuclear localization signal for which Lys379 and Arg380 are essential. (2006)
  • Author(s): Kitamura, R., Sekimoto, T., Ito, S., Harada, S., Yamagata, H., Masai, H., Yoneda, H., Yanagi, K.
  • Journal Title: J.Virol. 80 Pages: 1979-1991
• [Journal Article] Cell cycle and developmental regulations of replication factors in mouse embryonic stem cells. (2005)
  • Author(s): Fujii-Yamamoto, H., Kim, J-M., Arai, K., Masai, H.
  • Journal Title: J. Biol. Chem. 280 Pages: 12976-12987
  • Peer Reviewed
• [Journal Article] A second human Dbf4/ASK-related protein, Drf1/ASKL1 is required for efficient completion of S and M phases. (2005)
  • Author(s): Yoshizawa-Sugata, N., Ishii, A., Taniyama, C., Matsui, E., Arai, K., Masai, H.
  • Journal Title: J. Biol. Chem. 280 Pages: 13062-13070
  • Peer Reviewed
• [Journal Article] Functional analyses of mouse ASK, an activator subunit for Cdc7 kinase, using conditional ASK knockout ES cells. (2005)
  • Author(s): Yamashita, N., Kim, J-M., Koiwai, O., Arai, K., Masai, H.
  • Journal Title: Genes to Cells 50 Pages: 551-563
  • Peer Reviewed
• [Journal Article] Cell cycle and developmental regulations of replication factors in mouse embryonic stem cells. (2005)
  • Author(s): Fujii-Yamamoto, H., Kim, J-M., Arai, K., Masai, H.
  • Journal Title: J.Biol.Chem. 280 Pages: 12976-12987
• [Journal Article] A second human Dbf4/ASK-related protein, Drf1/ASKL1 is required for efficient completion of S and M phases. (2005)
  • Author(s): Yoshizawa, N., Ishii, A., Taniyama, C., Matsui, E., Arai, K., Masai, H.
  • Journal Title: J.Biol.Chem. 280 Pages: 13062-13070
• [Journal Article] DNA binding and helicase actions of mouse Mcm4/6/7 helicase. (2005)
  • Author(s): You, Z., Masai, H.
  • Journal Title: Nucl.Acids Res. 33 Pages: 3033-3047
• [Journal Article] Novel human origins of DNA replication selected from a library of nascent DNA. (2005)
  • Author(s): Todorovic, V., Giadrossi, S., Pelizon, C., Masai, H., Giacca, M.
  • Journal Title: Mol.Cell 19 Pages: 567-575
• [Journal Article] Hsk1-Dfp1/Him1, the Cdc7-Dbf4 Kinase in Schizosaccharowyces pombe, associates with Swi1, a component of the replication fork protection complex. (2005)
  • Author(s): Matsumoto, S., Ogino, K., Noguchi, E., Russell, P., Masai, H.
  • Journal Title: J.Biol.Chem. 280 Pages: 42536-42542
• [Journal Article] Control of DNA replication : regulation and activation of eukaryotic replicative helicase, MCM. (2005)
  • Author(s): Masai, H., You, Z., Arai, K.
  • Journal Title: IUBMB Life 57 Pages: 323-335
• [Presentation] DNA複製制御因子を標的とした制がん戦略:Cdc7キナーゼ阻害によるがん細胞死誘導 (2011)
  • Author(s): 正井久雄
  • Organizer: 第9回日本臨床腫瘍学会/日本癌学会/分子標的治療学会合同シンポジウム"基礎から学ぶ次世代の分子標的治療薬と基盤技術"
  • Place of Presentation: 東京(招待講演)
  • Year and Date: 2011-07-21
• [Presentation] Regulation of replication program in fission yeast and human cells (2011)
  • Author(s): Hisao Masai, Satoshi Yamazaki, Yutaka Kanoh, Motoshi Hayano, Seiji Matsumoto, Masako Oda, Naoko Kakusho, Rino Fukatsu, Michie Shimmoto, Naoko Yoshizawa
  • Organizer: 日本分子生物学会総会シンポジウム
  • Place of Presentation: 横浜(招待講演)
• [Presentation] 未分化胚性幹細胞の自己増幅と分化誘導を制御する細胞周期制御機構 (2010)
  • Author(s): 正井久雄、藤井裕子、加納豊、伊藤さゆり、沢野朝子、宮脇敦史
  • Organizer: 第9回日本再生医療学会総会
  • Place of Presentation: 広島
• [Presentation] Factors Regulating the Origin Selection in Eukaryotic DNA Replication. (2009)
  • Author(s): Masai, H., Matsumoto, S., Hayano, M., Kanoh, Y., Yamazaki, S., Shimmoto, M., Kakusho, N., Kudo, S.
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 横浜(シンポジウム招待講演)
• [Presentation] Cellular responses to stalled replication forks: roles of Cdc7 kinase and fork stabilization factors. (2009)
  • Author(s): Masai, H.
  • Organizer: 25th RBC International Symposium "Genomic Instability: mechanisms and diseases"
  • Place of Presentation: Kyoto (Invited Lecture)
• [Presentation] Actions of Mcm helicase and fork stabilizing factors at replication forks (MCMヘリカーゼと複製フォーク安定化因子の生化学的解析) (2009)
  • Author(s): Masai, H., You, Z., Tanaka, T., Yokoyama, M.
  • Organizer: 第82回日本生化学会大会
  • Place of Presentation: 神戸(招待講演)
• [Presentation] 複製開始部位の選択と活性化タイミングの遺伝的制御と可塑性. (2009)
  • Author(s): 正井久雄
  • Organizer: 日本遺伝学会第81回大会シンポジウム「日本のDNA複製研究の過去、現在、未来」
  • Place of Presentation: 信州大学理学部(長野県松本市)
• [Presentation] Actions of Mcm helicase and fork stabilizing factors at replication forks (2009)
  • Author(s): Masai, H., You, Z., Tanaka, T., Yokoyama, M.
  • Organizer: 第82回日本生化学会大会
  • Place of Presentation: 神戸
• [Presentation] Cellular responses to stalled replication forks : roles of Cdc7 kinase and fork stabilization factors. (2009)
  • Author(s): Masai, H.
  • Organizer: 25th RBC International Symposium "Genomic Instability : mechanisms and diseases"
  • Place of Presentation: Kyoto
• [Presentation] EBNA1-dependentassociation of mammalian pre-RC components with oriP of Epstein-Barr Virus in vitro (2009)
  • Author(s): 森山賢治, 高井裕子, 正井久雄
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 横浜
• [Presentation] Factors Regulating the Origin Selection in Eukaryotic DNA Replication. (2009)
  • Author(s): Masai, H., Matsumoto, S., Hayano, M., Kanoh, Y., Yamazaki, S., Shimmoto, M., Kakusho, N., Kudo, S.
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 横浜
• [Presentation] REGULATION OF ESTABLISHMENT AND STABLE MAINTENANCE OF REPLICATION FORKS BY CDC7 KINASE (2007)
  • Author(s): 正井 久雄
  • Organizer: 第30回日本分子生物学会年会、第80回日本生化学会大会 合同大会 BMB2007 シンポジウム
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2007-12-15
• [Book] 細胞工学31テロメア結合因子Rif1は、染色体複タイミングを決定する (2012)
  • Author(s): 早野元詞、加納豊、松本清治、正井久雄
  • Total Pages: 3
  • Publisher: 秀潤社
• [Book] 染色体サイクルの連係的制御機構概論蛋白質核酸酵素増刊号「染色体サイクル」(正井久雄、升方久夫、釣本敏樹、仁木宏典、篠原彰編集) (2009)
  • Author(s): 正井久雄
  • Publisher: 共立出版
• [Book] 序蛋白質核酸酵素増刊号「染色体サイクル」 (2009)
  • Author(s): 正井久雄
  • Total Pages: 1
  • Publisher: 共立出版
• [Book] 序論染色体サイクルの基礎.蛋白質核酸酵素増刊号「染色体サイクル」 (2009)
  • Author(s): 正井久雄
  • Total Pages: 6
  • Publisher: 共立出版
• [Book] 染色体サイクルの連係的制御機構概論蛋白質核酸酵素増刊号「染色体サイクル」 (2009)
  • Author(s): 正井久雄
  • Total Pages: 3
  • Publisher: 共立出版
• [Book] Cdc7キナーゼによる染色体サイクル制御.蛋白質核酸酵素増刊号「染色体サイクル」 (2009)
  • Author(s): 正井久雄
  • Total Pages: 7
  • Publisher: 共立出版
• [Book] S期開始,進行の制御と疾患.炎症と免疫 (2009)
  • Author(s): 正井久雄
  • Total Pages: 8
  • Publisher: 先端医学社
• [Book] 細胞周期を時空間的に可視化する技術.細胞工学、「細胞周期研究の新たなステージ」 (2009)
  • Author(s): 阪上-沢野朝子、正井久雄、宮脇敦史
  • Total Pages: 8
  • Publisher: 秀潤社
• [Book] 細胞周期を時空間的に可視化する技術.最新医学増刊号「幹細胞研究の最近の進歩」 (2009)
  • Author(s): 阪上-沢野朝子、正井久雄、宮脇敦史
  • Total Pages: 4
  • Publisher: 最新医学社
• [Book] 最新医学 増刊号「幹細胞研究の最近の進歩」.細胞周期を時空間的に可視化する技術 (2009)
  • Author(s): 坂上-沢野朝子、正井久雄、宮脇敦史
  • Total Pages: 4
  • Publisher: 最新医学社
• [Book] 細胞工学「細胞周期研究の新たなステージ」(岸本建雄 編集)28、細胞周期を時空間的に可視化する技術 (2009)
  • Author(s): 坂上-沢野朝子、正井久雄、宮脇敦史
  • Total Pages: 8
  • Publisher: 秀潤社
• [Book] S期開始・進行の制御と疾患、炎症と免疫 (2009)
  • Author(s): 正井久雄
  • Total Pages: 8
  • Publisher: 先端医学社
• [Book] Cdc7キナーゼによる染色体サイクル制御.蛋白質核酸酵素 増刊号「染色体サイクル」(正井久雄、升方久夫、釣本敏樹、仁木宏典、篠原彰編集) (2009)
  • Author(s): 正井久雄
  • Total Pages: 7
  • Publisher: 共立出版
• [Book] 染色体サイクルの連係的制御機構 概論 蛋白質核酸酵素 増刊号「染色体サイクル」(正井久雄、升方久夫、釣本敏樹、仁木宏典、篠原彰編集) (2009)
  • Author(s): 正井久雄
  • Total Pages: 3
  • Publisher: 共立出版
• [Book] 序論 染色体サイクルの基礎.蛋白質核酸酵素 増刊号「染色体サイクル」(正井久雄、升方久夫、釣本敏樹、仁木宏典、篠原彰 編集) (2009)
  • Author(s): 正井久雄
  • Total Pages: 6
  • Publisher: 共立出版
• [Book] 序 蛋白質核酸酵素 増刊号「染色体サイクル」(正井久雄、升方久夫、釣本敏樹、仁木宏典、篠原彰 編集) (2009)
  • Author(s): 正井久雄
  • Total Pages: 307
  • Publisher: 共立出版
• [Book] DNA複製開始と進行のゲノムワイドのプロファイル解析.細胞工学「細胞増殖とゲノム安定性維持のかなめ、DNA複製のメカニズム解明に迫る」(正井久雄 編集)27 (2008)
  • Author(s): 加納豊、正井久雄、白髭克彦
  • Total Pages: 5
  • Publisher: 秀潤社
• [Book] 序 DNA複製研究の過去、現在、未来.細胞工学「細胞増殖とゲノム安定性維持のかなめ、DNA複製のメカニズム解明に迫る」(正井久雄 編集)27 (2008)
  • Author(s): 正井久雄
  • Total Pages: 5
  • Publisher: 秀潤社
• [Book] 細胞周期をリアルタイムに可視化する技術.実験医学、増刊号「生命現象の動的理解を目指すライブイメージング」(宮脇敦史 編集) (2008)
  • Author(s): 阪上-沢野朝子、正井久雄、宮脇敦史
  • Total Pages: 8
  • Publisher: 羊土社
• [Book] "Regulation of DNA replication factors by E2F in cancer and embryonic stem cells" in "Control of Cellular Physiology by E2F Transcription Factors" (2008)
  • Author(s): Fujii-Yamamoto, H., Yamada, M., and Masai, H.
  • Total Pages: 12
  • Publisher: Research Signpost
• [Book] 「染色体サイクル:複製・分配・組換え・修復・クロマチン制御のメカニズムとその異常による疾患」序文 実験医学増刊号 (2007)
  • Author(s): 正井 久雄
  • Total Pages: 1
  • Publisher: 羊土社
• [Book] 概論"染色体サイクル制御の分子メカニズム"実験医学増刊号「染色体サイクル」 (2007)
  • Author(s): 正井 久雄、渡邊 嘉典
  • Total Pages: 8
  • Publisher: 羊土社
• [Book] "MCMタンパク質のDNA複製における役割"実験医学増刊号「染色体サイクル」 (2007)
  • Author(s): You Zhiying、 正井 久雄
  • Total Pages: 5
  • Publisher: 羊土社
• [Book] "Cdc7キナーゼによる複製フォーク制御"実験医学増刊号「染色体サイクル」 (2007)
  • Author(s): 正井 久雄、松本 清治
  • Total Pages: 8
  • Publisher: 羊土社
• [Book] 生化学辞典(分担)第4版 (2007)
  • Author(s): 正井 久雄
  • Publisher: 東京化学同人
• [Book] "Southwestern法"「分子間相互作用解析ハンドブック」 (2007)
  • Author(s): 田中 卓、正井 久雄
  • Total Pages: 4
  • Publisher: 羊土社
• [Book] "UVクロスリンキング法"「分子間相互作用解析ハンドブック」 (2007)
  • Author(s): You Zhiying, 正井 久雄
  • Total Pages: 4
  • Publisher: 羊土社
• [Book] 染色体サイクル : 複製・分配・組換え・修復・クロマチン制御のメカニズムとその異常による疾患序文 実験医学 増刊号 染色体サイクル (2007)
  • Author(s): 正井久雄
  • Total Pages: 219
  • Publisher: 羊土社
• [Book] 概論"染色体サイクル制御の分子メカニズム"実験医学 増刊号「染色体サイクル」 (2007)
  • Author(s): 正井久雄, 渡邊嘉典
  • Total Pages: 216
  • Publisher: 羊土社
• [Book] "MCMタンパク質のDNAZ複製における役割"実験医学 増刊号「染色体サイクル」 (2007)
  • Author(s): Zhiying You, 正井久雄
  • Total Pages: 219
  • Publisher: 羊土社
• [Book] Cdc7キナーゼによる複製フォーク制御"実験医学増刊号「染色体サイクル」 (2007)
  • Author(s): 正井久雄, 松本清治
  • Total Pages: 219
  • Publisher: 羊土社
• [Book] ヒトの分子生物学(村松正實 監訳) (2006)
  • Author(s): 正井久雄(翻訳分担)
  • Total Pages: 715
  • Publisher: 丸善株式会社
• [Book] 体細胞分裂に重要な役割を果たす分裂酵母Cdc7類似キナーゼ, Hsk1タンパク質は減数分裂期組換えの開始に必要とされる(細胞工学) (2006)
  • Author(s): 荻野桂子, 廣田耕志, 太田邦史, 正井久雄
  • Publisher: 秀潤社
• [Remarks]
  • URL: http://www.nig.ac.jp/labs/NigPrjct/sensai/vol01/index.html
• [Remarks]
  • URL: http://www.rinshoken.or.jp/CB/index-jp.htm
• [Remarks]
  • URL: http://www.rinshoken.or.jp/CB/index-jp.htm