mechanisms of chromosome regulation by Cdc7 kinase

• Project/Area Number: 17080015
• Research Category: Grant-in-Aid for Scientific Research on Priority Areas
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: 財団法人東京都医学研究機構 (2009); Tokyo Metropolitan Organization for Medical Research (2005-2008)
• Principal Investigator: MASAI Hisao 財団法人東京都医学研究機構, 東京都臨床医学総合研究所, 参事研究員 (40229349)
• Project Period (FY): 2005 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥156,400,000 (Direct Cost: ¥156,400,000); Fiscal Year 2009: ¥30,400,000 (Direct Cost: ¥30,400,000); Fiscal Year 2008: ¥30,400,000 (Direct Cost: ¥30,400,000); Fiscal Year 2007: ¥30,400,000 (Direct Cost: ¥30,400,000); Fiscal Year 2006: ¥30,400,000 (Direct Cost: ¥30,400,000); Fiscal Year 2005: ¥34,800,000 (Direct Cost: ¥34,800,000)
• Keywords: DNA複製 / Cdc7キナーゼ / リン酸化 / 組換え開始 / 染色体サイクル / 複製フォー / ク停止 / DNA修復 / 染色体分配 / 染色体ダイナミクス / Clasin/Mrc1 / 細胞死誘導 / がん細胞 / 複製フォーク / 変異細胞株 / 染色体安定性 / 染色体接着 / 減数分裂期組換え / チェックポイント / ヘテロクロマチン形成 / DNA / 複製

Research Abstract

Cdc7 kinase plays critical roles in initiation of DNA replication. Additional important functions of Cdc7 in other chromosome transactions have been suggested. This includes meiotic recombination, DNA damage bypass (Trans-legion synthesis), and DNA replication checkpoint and so forth. We identified critical substrates of Cdc7 kinase in these processes. We also identified novel fission yeast mutations that can bypass the requirement of Hsk1 kinase for the growth. Characterization of these mutants lead to identification of some of the novel mechanisms that contribute to the plastic regulation of timing and site selection of origin firing.

Research Products

• [Journal Article] Pre-firing binding of Mrcl defines the early-firing origins which are selectively hyper-activated upon loss of fork stabilizing factors in fission yeast. (2011)
  • Author(s): Hayano, M., Kanoh, Y., Matsumoto, S., Kakusho, N., Masai, H.
  • Journal Title: Mol.Cell.Biol. 31 Pages: 2380-2391
• [Journal Article] Molecular mechanism of activation of human Cdc7 kinase : Bipartite interaction with Dbf4/ASK stimulates ATP binding and substrate recognition. (2011)
  • Author(s): Ryo Kitamura, Rino Fukatsu, Naoko Kakusho, Yong-Soon Cho, Chika Taniyama, Satoshi Yamazaki, Gaik-theng Toh, Kazuo Yanagi, Naoko Arai, Ho-Jin Chang, Hisao Masai
  • Journal Title: J.Biol.Chem. (In press)
• [Journal Article] Efficient expression and purification of human replication fork-stabilizing factor, Claspin, from mammalian cells : DNA binding activity and novel protein interactions. (2011)
  • Author(s): Uno, S., Masai, H.
  • Journal Title: Genes to Cells. (In press)
• [Journal Article] RecQL4 : a helicase linking formation and maintenance of a replication fork. (2011)
  • Author(s): Masai, H.
  • Journal Title: J.Biochem. (In press (commentary) )
  • NAID: 10030585512
• [Journal Article] Cdc7L1. (2011)
  • Author(s): Toh G-T., Masai, H.
  • Journal Title: UCSD-Nature Molecule Pages (in press (Review))
• [Journal Article] DDK phosphorylates checkpoint clamp Rad9 and promotes its release from damaged Chromatin. (2010)
  • Author(s): Furuya, K., Miyabe, I., Tsutsui, Y., Paderi, F., Kakusho, N., Masai, H., Niki, H., Carr, A.M.
  • Journal Title: Mol.Cell 40 Pages: 606-618
• [Journal Article] Cdc7-Mediated Radl8 Phosphorylation Directs the Accumulation of DNA Polymerase i1 at Sites of Stalled Replication. (2010)
  • Author(s): Day, T.A., Palle, K., Barkley, L.R., Kakusho, N., Zou, Y., Tateishi, S., Verreault, A., Masai, H., Vaziri, C.
  • Journal Title: J.Cell Biol. 191 Pages: 953-966
• [Journal Article] Hskl kinase and Cdc45 regulate replication stress-induced checkpoint responses in fission yeast. (2010)
  • Author(s): Matsumoto, S., Shimmoto, M., Kakusho, N., Yokoyama, M., Russell, P., Masai, H.
  • Journal Title: Cell Cycle 9 Pages: 4627-4637
• [Journal Article] Fission yeast Swil-Swi3 complex facilitates DNA binding of Mrc 1. (2010)
  • Author(s): Tanaka, T., Yokoyama, M., Matsumoto, S., Fukatsu, R., You, Z., う.
  • Journal Title: J.Biol.Chem. 285 Pages: 39609-39622
• [Journal Article] Eukaryotic DNA replication; where, when and how? (2010)
  • Author(s): Masai, H., Matsumoto, S., You, Z., Yoshizawa-Sugata, N., Oda, M.
  • Journal Title: Annual Rev.Biochem. 79 Pages: 89-130
• [Journal Article] Stalled replication forks : Making ends meet for recognition and stabilization. (2010)
  • Author(s): Masai, H., Tanaka, T., Kohda, D.
  • Journal Title: Bioessays(Review) 32, Pages: 687-697
• [Journal Article] Integrating DNA replication with Trans-Lesion Synthesis via Cdc7. (2010)
  • Author(s): Vaziri, C., Masai, H.
  • Journal Title: Cell Cycle Dec([Epub ahead of print] PMID : 21150323) 9(24) Pages: 15-15
• [Journal Article] Fission yeast Swil-Swi3 complex facilitates DNA binding of Mrcl. (2010)
  • Author(s): Tanaka, T., Yokoyama, M., Matsumoto, S., Fukatsu, R., You, Z., Masai, H.
  • Journal Title: J.Biol.Chem. Volume: 285 Pages: 39609-39622
  • Peer Reviewed
• [Journal Article] Deregulated Cdc6 inhibits DNA replication and suppresses Cdc7-mediated phosphorylation of Mcm2-7 complex. (2010)
  • Author(s): Kundu,L.R., Kumata, Y, Kakusho, N., Watanabe, S., Furukohri, A., Waga, S., Sekia, M., Masai, H., Enomoto, T., Tada, S.
  • Journal Title: Nucleic Acid Res. Pages: 5409-5418
  • Peer Reviewed
• [Journal Article] Casein kinase 2-dependent phosphorylation of human Rad9 mediates the interaction between human Rad9-Hus1-Rad1 complex and TopBP1. (2010)
  • Author(s): Takeishi, Y., Ohashi, E., Ogawa, K, Masai, H., Obuse, C., Tsurimoto, T.
  • Journal Title: Genes Cells Volume: 15 Pages: 761-771
  • NAID: 120005227474
  • Peer Reviewed
• [Journal Article] DDK phosphorylates checkpoint clamp Rad9 and promotes its release from damaged Chromatin. (2010)
  • Author(s): Furuya, K., Miyabe, I., Tsutsui, Y., Paderi, F., Kakusho, N., Masai, H., Niki, H., Carr, A.M.(20
  • Journal Title: Mol.Cell Volume: 40 Pages: 606-618
  • Peer Reviewed
• [Journal Article] Cdc7-Mediated Rad18 Phosphorylation Directs the Accumulation of DNA Polymerase η at Sites of Stalled Replication. (2010)
  • Author(s): Day, T.A., Palle, K., Barkley, L.R., Kakusho, N., Zou, Y., Tateishi, S., Verreault, A., Masai, H., Vaziri, C.
  • Journal Title: J.Cell Biol. Volume: 191 Pages: 953-966
  • Peer Reviewed
• [Journal Article] Hsk1 kinase and Cdc45 regulate replication stress-induced checkpoint responses in fission yeast." (2010)
  • Author(s): Matsumoto, S., Shimmoto, M., Kakusho, N., Yokoyama, M., Russell, P., Masai, H.
  • Journal Title: Cell Cycle Volume: 9 Pages: 4627-4637
  • Peer Reviewed
• [Journal Article] FANCs regulate firing of DNA replication origins. (2010)
  • Author(s): Masai, H.
  • Journal Title: Cell Cycle Volume: 9 Pages: 2494-2494
  • Peer Reviewed
• [Journal Article] Integrating DNA replication with Trans-Lesion Synthesis via Cdc7. (2010)
  • Author(s): Vaziri, C., Masai H.
  • Journal Title: Cell Cycle Volume: 9 Pages: 4818-4823
  • Peer Reviewed
• [Journal Article] Eukaryotic DNA replication ; where, when and how? (2010)
  • Author(s): Masai, H., Matsumoto, S., You, Z., Sugata-Yoshizawa, N., Oda, M.
  • Journal Title: Annual.Rev.Biochem. Volume: 79 Pages: 89-130
  • Peer Reviewed
• [Journal Article] Stalled replication forks : Making ends meet for recognition and stabilization. (2010)
  • Author(s): Masai, H., Tanaka, T., Kohda, D.
  • Journal Title: BioEssays Volume: 32 Pages: 687-697
  • Peer Reviewed
• [Journal Article] Bacterial primosome. (2010)
  • Author(s): Tanaka, T., Masai, H.
  • Journal Title: Encyclopedia of Life Sciences
  • Peer Reviewed
• [Journal Article] Bacterial replication fork. (2010)
  • Author(s): Tanaka, T., Masai, H.
  • Journal Title: Encyclopedia of Life Sciences
  • Peer Reviewed
• [Journal Article] Eukaryotic chromosome DNA replication : where, when, and how? (2010)
  • Author(s): Masai, H., Matsumoto, S., You, Z., Yoshizawa-Sugata, N., Oda, M.
  • Journal Title: Annu.Rev.Biochem. 79 Pages: 89-130
  • Peer Reviewed
• [Journal Article] Deregulated Cdc6 inhibits DNA replication and suppresses Cdc7-mediated phosphorylation of Mcm2-7 complex. (2010)
  • Author(s): Kundu, L..R., Kumata, Y., Kakusho, N., Watanabe, S., Furukohri, A., Waga, S., Sekia, M., Masai, H., Enomoto, T., Tada, S.
  • Journal Title: Nucleic Acid Res. (In press)
  • Peer Reviewed
• [Journal Article] Casein Kinase 2-dependent phosphorylation of human Rad9 mediates the interaction between human Rad9-Husl-Radl complex and TopBP1. (2010)
  • Author(s): Takeishi, Y., Ohashi, E., Ogawa, K., Masai, H., Obuse, C., Tsurimoto, T.
  • Journal Title: Genes to Cells (In press)
• [Journal Article] Interactions between Swil-Swi3, Mrcl and S phase kinase, Hskl may regulate cellular responses to stalled replication forks in fission yeast. (2009)
  • Author(s): Shimmoto, S., Matsumoto, S., Hayano, M., Yokoyama, M., Noguchi, E., Russell, P., Masai, H.
  • Journal Title: Genes to Cells 14 Pages: 669-682
• [Journal Article] Drug Design with Cdc7 kinase, a potential novel cancer therapy target. (2009)
  • Author(s): Sawa, M., Masai, H.
  • Journal Title: Drug Design, Development and Therapy 2 Pages: 255-264
• [Journal Article] Replisome progression complex links DNA replication to sister chromatid cohesion in Xenopus egg extracts. (2009)
  • Author(s): Tanaka, H., Kubota, Y., Tsujimura, T., Kumano, M., Masai, H., Takisawa, H.
  • Journal Title: Genes to Cells 14 Pages: 949-963
  • Peer Reviewed
• [Journal Article] Roles of human AND-1 in chromosome transactions in S phase. (2009)
  • Author(s): Yoshizawa-Sugata, N., Masai, H.
  • Journal Title: J.Biol.Chem. 284 Pages: 20718-20728
  • Peer Reviewed
• [Journal Article] ASK/Dbf4 (2009)
  • Author(s): Toh, G.K., Masai, H.
  • Journal Title: UCSD-Nature Molecule Pages (On line)
  • Peer Reviewed
• [Journal Article] Cdc7 kinase is required for Claspin phosphorylation in DNA replication checkpoint. (2008)
  • Author(s): Kim, J-M., Kakusho, N., Yamada, M., Kanoh, Y., Takemoto, N., Masai, H.
  • Journal Title: Oncogene 27 Pages: 3475-3482
• [Journal Article] Cdc7-dependent phosphorylation of Mer2 facilitates initiation of yeast meiotic recombination. (2008)
  • Author(s): Sasanuma, H., Hirota, K., Fukuda, T., Kakusho, N., Kugou, N., Kawasaki, Y., Shibata, T., Masai, H Ohta, K.
  • Journal Title: Genes & Dev. 22 Pages: 398-410
• [Journal Article] Spatio-temporal dynamics of multicellular cell cycle progression. (2008)
  • Author(s): Sakaue-Sawano, A., Kurokawa, H., Morimura, T., Hanyu, A., Hama, H., Kashiwagi, S., Fukami, K., Imamura, T., Ogawa, M., Masai, H., Miyawaki, A.
  • Journal Title: Featured on the cover of the issue ell 132 Pages: 487-498
• [Journal Article] Cdc7 as a potential new target for cancer therapy. (2008)
  • Author(s): Ito, S., Taniyama, C., Arai, N., Masai, H.
  • Journal Title: Drug News and Perspectives(Featured on the cover of the issue) 21 Pages: 481-488
• [Journal Article] Cdc7-dependent phosphorylation of Mer2 facilitates initiation of yeast meiotic recombination. (2008)
  • Author(s): Sasanuma, H., Hirota, K., Fukuda, T., Kakusho, N., Kugou, N., Kawasaki, Y., Shibata, T., Masai, H., Ohta, K.
  • Journal Title: Genes & Dev. 22 Pages: 398-410
  • Peer Reviewed
• [Journal Article] Spatio-temporal dynamics of multicellular cell cycle progression. (2008)
  • Author(s): Sakaue-Sawano, A., Kurokawa, H., Morimura, T., Hanyu, A., Hama, H., Kashiwagi, S., Fukami, K., Imamura, T., Ogawa , M., Masai, H., Miyawaki, A.
  • Journal Title: Cell 132 Pages: 487-498
  • Peer Reviewed
• [Journal Article] Identification of stimulators and inhibitors of CDC7 kinase in vitro. (2008)
  • Author(s): Kakusho, N., Taniyama, C., Masai, H.
  • Journal Title: J.Biol.Chem. 283 Pages: 19211-19218
  • Peer Reviewed
• [Journal Article] Cdtl forms a complex with MCM and activates its helicase activity. (2008)
  • Author(s): You, Z., Masai, H.
  • Journal Title: J.Biol.Chem. 283 Pages: 24469-24477
  • Peer Reviewed
• [Journal Article] 細胞周期をリアルタイムに可視化する技術 (2008)
  • Author(s): 阪上-沢野朝子、正井久雄、宮脇敦史
  • Journal Title: 実験医学、増刊号「生命現象の動的理解を目指すライブイメージング」 26 Pages: 148-155
  • Peer Reviewed
• [Journal Article] 序DNA複製研究の過去、現在、未来 (2008)
  • Author(s): 正井久雄
  • Journal Title: 細胞工学「細胞増殖とゲノム安定性維持のかなめ、DNA複製のメカニズム解明に迫る 27 Pages: 962-966
  • Peer Reviewed
• [Journal Article] DNA複製開始と進行のゲノムワイドのプロファイル解析 (2008)
  • Author(s): 加納豊、正井久雄、白髭克彦
  • Journal Title: 細胞工学「細胞増殖とゲノム安定性維持のかなめ、DNA複製のメカニズム解明に迫る」 27 Pages: 1008-1012
  • Peer Reviewed
• [Journal Article] Identification of stimulators and inhibitors of CDC7 kinase in vitro (2008)
  • Author(s): Kakusho, N., Taniyama, C., and Masai, H.
  • Journal Title: J.Biol.Chem 282(印刷中)
  • Peer Reviewed
• [Journal Article] Spatio-tempqral dynamics of multicellular cell cycle progression (2008)
  • Author(s): Sakaue-Sawano, A., Kurokawa, H., Morimura, T., Hanyu, A., Hama, H., Kashiwagi, S., Fukami, K., Imamura, T., Ogawa, M., Masai, H. and Miyawaki, A.
  • Journal Title: Cell 132 Pages: 487-498
  • Peer Reviewed
• [Journal Article] Cdc7-dependent phosphorylation of Mer2 facilitates initiation of yeastmeiotic recombination (2008)
  • Author(s): Sasanuma, H., Hirota, K., Fukuda, T., Kakusho, N., Kugou, N., Kawasaki, Y., Shibata, T., Masai, H. and Ohta, K.
  • Journal Title: Genes Dev 22 Pages: 398-410
  • Peer Reviewed
• [Journal Article] Cdc7 kinase is required for Claspin phosphorylation in DNA replication checkpoint (2008)
  • Author(s): Kim, J-M., Yamada, M., Kakusho, N., Kanoh, Y., Takemoto, N., and Masai, H.
  • Journal Title: Oncogene 27(印刷中)
  • Peer Reviewed
• [Journal Article] Structural basis of the3'-end recognition of a leading strand in stalled DNA replication forks by PriA (2007)
  • Author(s): Sasaki, K., Ose, T., Okamoto, N., Maenaka, K., Tanaka, T., Masai, H., Saito, M., Shirai, T.and Kohda, D.
  • Journal Title: EMBO J 26 Pages: 2584-2593
  • Peer Reviewed
• [Journal Article] Human Tim/Timeless-interacting protein, Tipin, is required for efficient progression of S phase and DNA replication checkpoint (2007)
  • Author(s): Yoshizawa-Sugata, N. and Masai, H.
  • Journal Title: J. Biol. Chem 282 Pages: 2729-2740
  • Peer Reviewed
• [Journal Article] Escherichia coli PriA protein: Two modes of DNA binding and activation of ATP hydrolysis (2007)
  • Author(s): Tanaka, T., Mizukoshi, T., Sasaki, K., Kohda, D. and Masai, H.
  • Journal Title: J. Biol. Chem 282 Pages: 19917-19927
  • Peer Reviewed
• [Journal Article] Structural basis of the 3'-end recognition of a leading strand in stalled DNA replication forks by PriA. (2007)
  • Author(s): Sasaki, K.
  • Journal Title: EMBO J. (印刷中)
• [Journal Article] Escherichia coli PriA protein : Two modes of DNA binding and activation. of ATP hydrolysis. (2007)
  • Author(s): Tanaka, T.
  • Journal Title: J. Biol. Chem. (印刷中)
• [Journal Article] Human Tim/Timeless-interacting protein, Tipin, is required for efficient progression of S phase and DNA replication checkpoint. (2007)
  • Author(s): Yoshizawa-Sugata, N.
  • Journal Title: J. Biol. Chem. 282 Pages: 2729-2740
• [Journal Article] Identification of stimulators and inhibitors of CDC7 kinase in vitro. (2006)
  • Author(s): Kakusho, N., Taniyama, C., Masai, H.
  • Journal Title: Proc.Natl.Acad.Sci.USA 103 Pages: 8131-8136
• [Journal Article] Phosphorylation of MCM4 by Cdc7 kinase facilitateswith Cdc45 on the chromatin. (2006)
  • Author(s): Masai, H., Taniyama, C., Ogino, K., Matsui, E., Kakusho, N., Matsumoto, M., Kim, J-M., Ishii, A., Tanaka, T., Kobayashi, T., Tamai, K., Ohtani, K., Arai, K.
  • Journal Title: J.Biol.Chem. 281 Pages: 39249-32961
• [Journal Article] Phosphorylation of MCM4 by Cdc7 kinase facilitates its interaction with Cdc45 on the chromatin. (2006)
  • Author(s): Masai, H.
  • Journal Title: J. Biol. Chem. 281 Pages: 39249-32961
• [Journal Article] Replication initiation from a novel origin identified in the Th2 cytokine cluster locus requires a distant conserved non-coding sequence. (*cocommunicating authors) (2006)
  • Author(s): Hayashida, T.
  • Journal Title: J. Immunol. 176 Pages: 5446-5454
• [Journal Article] Rad3-Cds1 mediates coupling of initiation of meiotic recombination with DNA replication : Mei4-dependent transcription as a potential target of meiotic checkpoint. (2006)
  • Author(s): Ogino, K.
  • Journal Title: J. Biol. Chem. 281 Pages: 1338-1344
• [Journal Article] Stabilization of a stalled replication fork by concerted actions of two helicases. (2006)
  • Author(s): Tanaka, T.
  • Journal Title: J. Biol. Chem. 281 Pages: 3484-3493
• [Journal Article] Crystallization and preliminary crystallographic analysis of the N-terminal domain of PriA from Escherichia coli. (2006)
  • Author(s): Sasaki, K.
  • Journal Title: Biochim. Biophys. Acta. 1764 Pages: 157-160
• [Journal Article] Nuclear import of Epstein-Barr Virus Nuclear Antigen 1 mediated by NPI-1 (Importin α5) is up- and down-regulated by phosphorylation of the nuclear localization signal for which Lys379 and Arg380 are essential. (2006)
  • Author(s): Kitamura, R.
  • Journal Title: J. Virol. 80 Pages: 1979-1991
• [Journal Article] Hsk1 kinase is required for induction of meiotic double-stranded DNA breaks without involving checkpoint kinases in fission yeast.accepted for publication (2006)
  • Author(s): Ogino, K., Hirota, K., Matsumoto, S., Takeda, T., Ohta, K., Arai, K., Masai, H.
  • Journal Title: Proc.Natl.Acad.Sci.USA (印刷中)
• [Journal Article] "Replication initiation from a novel origin identified in the Th2 cytokine cluster locus requires a distant conserved non-coding sequence." (*cocommunicating authors) (2006)
  • Author(s): Hayashida, T., Oda, M., Ohsawa, K., Yamaguchi A., Giacca, M., Locksley, R.M., Masai H., Miyatake, S.
  • Journal Title: J.Immunol. (印刷中)
• [Journal Article] Rad3-Cds1 mediates coupling of initiation of meiotic recombination with DNA replication : Mei4-dependent transcription as a potential target of meiotic checkpoint. (2006)
  • Author(s): Ogino, K., Masai, H.
  • Journal Title: J.Biol.Chem. 281 Pages: 1338-1344
• [Journal Article] Stabilization of a stalled replication fork by concerted actions of two helicases. (2006)
  • Author(s): Tanaka, T., Masai, H.
  • Journal Title: J.Biol.Chem. 281 Pages: 3484-3493
• [Journal Article] Crystallization and preliminary crystallographic analysis of the N-terminal domain of PriA from Escherichia coli. (2006)
  • Author(s): Sasaki, K., Ose, T., Tanaka, T., Mizukoshi, T., Ishigaki, T., Maenaka, K., Masai, H., Kohda, D.
  • Journal Title: Biochim.Biophys.Acta. 1764 Pages: 157-160
• [Journal Article] Nuclear import of Epstein-Barr Virus Nuclear Antigen 1 mediated by NPI-1 (Importin ・5) is up- and down-regulated by phosphorylation of the nuclear localization signal for which Lys379 and Arg380 are essential. (2006)
  • Author(s): Kitamura, R., Sekimoto, T., Ito, S., Harada, S., Yamagata, H., Masai, H., Yoneda, H., Yanagi, K.
  • Journal Title: J.Virol. 80 Pages: 1979-1991
• [Journal Article] This paper was selected as "JBC paper of the week" and was featured in the cover of December 22 issue of JBC. A second human Dbf4/ASK-related protein, Drfl/ASKL1 is required for efficient completion of S and M phases. (2005)
  • Author(s): Yoshizawa-Sugata, N., Ishii, A., Taniyama, C., Matsui, E., Arai, K., Masai, H.
  • Journal Title: J.Biol.Chem. 280 Pages: 13062-13070
• [Journal Article] Functional analyses of mouse ASK, an activator subunit for Cdc7 kinase, using conditional ASK knockout ES cells. (2005)
  • Author(s): Yamashita, N., Kim, J-M., Koiwai, O., Arai, K., Masai, H.
  • Journal Title: Genes to Cells 50 Pages: 551-563
• [Journal Article] Novel human origins of DNA replication selected from a library of nascent DNA. (2005)
  • Author(s): Todorovic, V., Giadrossi, S., Pelizon, C., Masai, H., Giacca, M.
  • Journal Title: Mol.Cell 19 Pages: 567-575
• [Journal Article] Hskl-Dfpl/Himl, the Cdc7-Dbf4 Kinase in Schizosaccharomyces pombe, associates with Swi1, a component of the replication fork protection complex. (2005)
  • Author(s): Matsumoto, S., Ogino, K., Noguchi, E., Russell, P., Masai, H.
  • Journal Title: J.Biol.Chem. 280 Pages: 42536-42542
• [Journal Article] Cell cycle and developmental regulations of replication factors in mouse embryonic stem cells. (2005)
  • Author(s): Fujii-Yamamoto, H., Kim, J-M., Arai, K., Masai, H.
  • Journal Title: J.Biol.Chem. 280 Pages: 12976-12987
• [Journal Article] A second human Dbf4/ASK-related protein, Drf1/ASKL1 is required for efficient completion of S and M phases. (2005)
  • Author(s): Yoshizawa, N., Ishii, A., Taniyama, C., Matsui, E., Arai, K., Masai, H.
  • Journal Title: J.Biol.Chem. 280 Pages: 13062-13070
• [Journal Article] DNA binding and helicase actions of mouse Mcm4/6/7 helicase. (2005)
  • Author(s): You, Z., Masai, H.
  • Journal Title: Nucl.Acids Res. 33 Pages: 3033-3047
• [Journal Article] Novel human origins of DNA replication selected from a library of nascent DNA. (2005)
  • Author(s): Todorovic, V., Giadross i, S., Pelizon, C., Masai, H., Giacca, M.
  • Journal Title: Mol.Cell 19 Pages: 567-575
• [Journal Article] Hsk1-Dfp1/Him1, the Cdc7-Dbf4 Kinase in Schizosaccharomyces pombe, associates with Swi1, a component of the replication fork protection complex. (2005)
  • Author(s): Matsumoto, S., Ogino, K., Noguchi, E., Russell, P., Masai, H.
  • Journal Title: J.Biol.Chem. 280 Pages: 42536-42542
• [Journal Article] Control of DNA replication : regulation and activation of eukaryotic replicative helicase, MCM. (2005)
  • Author(s): Masai, H., You, Z., Arai, K.
  • Journal Title: IUBMB Life 57 Pages: 323-335
• [Journal Article] ASK
  • Author(s): Toh, G.K., Masai, H.
  • Journal Title: UCSD-Nature Molecule Pages(Review)
• [Presentation] Toward understanding the regulation of chromosome cycle by Cdc7 kinase. (2010)
  • Author(s): Masai, H.,(Organizer and speaker)
  • Organizer: The final symposium on Chromosome Cycle
  • Place of Presentation: Hongo, Tokyo, Japan
  • Year and Date: 2010-10-01
• [Presentation] Regulation of replication program and maintenance of genetic integrity. (2010)
  • Author(s): Masai, H., Yamazaki, S., Hayano, M., Kanoh, Y., Matsumoto, S., Kakusho, N., Ishii, A., Shimmoto, M, Oda, M., Yoshizawa, N., Ito, S., Kudo, S.(Organizer and speaker)
  • Organizer: BMB2010 Symposium
  • Place of Presentation: Kobe, Japan
• [Presentation] Cell Cycle and Mitosis. (2010)
  • Author(s): Masai, H.: 2010.11.24-30, RIKEN Yokohama, Japan
  • Organizer: Karolinska Institute RIKEN Joint Course 'Functional architecture of the cell nucleus'
  • Place of Presentation: RIKEN Yokohama(Invited lecture)
• [Presentation] Cell cycle regulation of embryonic stem cells regulating the maintenance of pluripotency and differentiation. (2010)
  • Author(s): Masai, H., Fujii, H., Kanoh, Y., Kakusho, N., Ito, S., Sawano, A., Miyawaki, A.
  • Organizer: The 53^<rd> annual meeting of the Japan Radiation Society.(日本放射線影響学会)
  • Place of Presentation: Kyoto, Japan(Invited lecture)
• [Presentation] 未分化胚性幹細胞の自己増幅と分化誘導を制御する細胞周期制御機構 (2010)
  • Author(s): 正井久雄、藤井裕子、加納豊、伊藤さゆり、沢野朝子、宮脇敦史
  • Organizer: 第9回日本再生医療学会総会
  • Place of Presentation: 広島
• [Presentation] Cellular responses to stalled replication forks : roles of Cdc7 kinase and fork stabilization factors. (2009)
  • Author(s): Masai, H.
  • Organizer: 25th RBC International Symposium "Genomic Instability : mechanisms and diseases"
  • Place of Presentation: Kyoto
  • Year and Date: 2009-11-30
• [Presentation] Actions of Mcm helicase and fork stabilizing factors at replication forks (2009)
  • Author(s): Masai, H., You, Z., Tanaka, T., Yokoyama, M.
  • Organizer: 第82回日本生化学会大会
  • Place of Presentation: 神戸
  • Year and Date: 2009-10-24
• [Presentation] 複製開始部位の選択と活性化タイミングの遺伝的制御と可塑性 (2009)
  • Author(s): 正井久雄
  • Organizer: 日本遺伝学会第81回大会シンポジウム「日本のDNA複製研究の過去、現在、未来」
  • Place of Presentation: 信州大学理学部(長野県松本市)
  • Year and Date: 2009-09-17
• [Presentation] Regulation of replication fork integrity during S phase. (2008)
  • Author(s): Hisao Masai, Motoshi Hayano, Seiji Matsumoto, Yutaka Kanoh, Mika Yokoyama, Michiyo Shimmoto, Naoko Kakusho, Naoko Yoshizawa, Taku Tanaka
  • Organizer: BMB2008
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2008-12-11
• [Presentation] REGULATION OF ESTABLISHMENT AND STABLE MAINTENANCE OF REPLICATION FORKS BY CDC7 KINASE (2007)
  • Author(s): 正井 久雄
  • Organizer: 第30回日本分子生物学会年会、第80回日本生化学会大会 合同大会 BMB2007 シンポジウム
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2007-12-15
• [Book] なぜ親子は似るの?「なぜなぜ生物学」(東京化学同人) (2010)
  • Author(s): 正井久雄
  • Publisher: MBSJ日本分子生物学会編
• [Book] 生物学辞典(分担)東京化学同人 (2010)
  • Author(s): 正井久雄
• [Book] 細胞周期の時空間的解析.「細胞周期フロンティア」(佐方功幸、稲垣昌樹、岸本健雄編集) (2010)
  • Author(s): 阪上-沢野朝子、正井久雄、宮脇敦史
  • Publisher: 共立出版
• [Book] なぜ親子は似るの?「なぜなぜ生物学」(MBSJ日本分子生物学会編) (2010)
  • Author(s): 正井久雄
  • Total Pages: 182
  • Publisher: 東京化学同人
• [Book] 生物学辞典(分担) (2010)
  • Author(s): 正井久雄
  • Total Pages: 1615
  • Publisher: 東京化学同人
• [Book] 序蛋白質核酸酵素増刊号「染色体サイクル」(正井久雄、升方久夫、釣本敏樹、仁木宏典、篠原彰編集) (2009)
  • Author(s): 正井久雄
  • Total Pages: 307
  • Publisher: 共立出版
• [Book] 序論染色体サイクルの基礎蛋白質核酸酵素増刊号「染色体サイクル」(正井久雄、升方久夫、釣本敏樹、仁木宏典、篠原彰編集) (2009)
  • Author(s): 正井久雄
  • Publisher: 共立出版
• [Book] 染色体サイクルの連係的制御機構概論蛋白質核酸酵素増刊号「染色体サイクル」(正井久雄、升方久夫、釣本敏樹、仁木宏典、篠原彰編集) (2009)
  • Author(s): 正井久雄
  • Publisher: 共立出版
• [Book] Cdc7キナーゼによる染色体サイクル制御.蛋白質核酸酵素増刊号「染色体サイクル」(正井久雄、升方久夫、釣本敏樹、仁木宏典、篠原彰編集) (2009)
  • Author(s): 正井久雄
  • Publisher: 共立出版
• [Book] 序蛋白質核酸酵素増刊号「染色体サイクル」 (2009)
  • Author(s): 正井久雄
  • Total Pages: 1
  • Publisher: 共立出版
• [Book] 序論染色体サイクルの基礎.蛋白質核酸酵素増刊号「染色体サイクル」 (2009)
  • Author(s): 正井久雄
  • Total Pages: 6
  • Publisher: 共立出版
• [Book] 染色体サイクルの連係的制御機構概論蛋白質核酸酵素増刊号「染色体サイクル」 (2009)
  • Author(s): 正井久雄
  • Total Pages: 3
  • Publisher: 共立出版
• [Book] Cdc7キナーゼによる染色体サイクル制御.蛋白質核酸酵素増刊号「染色体サイクル」 (2009)
  • Author(s): 正井久雄
  • Total Pages: 7
  • Publisher: 共立出版
• [Book] S期開始,進行の制御と疾患.炎症と免疫 (2009)
  • Author(s): 正井久雄
  • Total Pages: 8
  • Publisher: 先端医学社
• [Book] 細胞周期を時空間的に可視化する技術.細胞工学、「細胞周期研究の新たなステージ」 (2009)
  • Author(s): 阪上-沢野朝子、正井久雄、宮脇敦史
  • Publisher: 季潤社
• [Book] 細胞周期を時空間的に可視化する技術.最新医学 増刊号「幹細胞研究の最近の進歩」 (2009)
  • Author(s): 阪上-沢野朝子、正井久雄、宮脇敦史
  • Publisher: 最新医学社
• [Book] 細胞周期をリアルタイムに可視化する技術.実験医学、増刊号「生命現象の動的理解を目指すライブイメージング」(宮脇敦史編集)26 (2008)
  • Author(s): 阪上-沢野朝子、正井久雄、宮脇敦史
  • Publisher: 羊土社
• [Book] 序DNA複製研究の過去、現在、未来.細胞工学「細胞増殖とゲノム安定性維持のかなめ、DNA複製のメカニズム解明に迫る、(正井久雄編集)27 (2008)
  • Author(s): 正井久雄
  • Publisher: 秀潤社
• [Book] DNA複製開始と進行のゲノムワイドのプロファイル解析.細胞工学「細胞増殖とゲノム安定性維持のかなめ、DNA複製のメカニズム解明に迫る」(正井久雄編集)27 (2008)
  • Author(s): 加納豊、正井久雄、白髭克彦
  • Publisher: 秀潤社
• [Book] "Regulation of DNA replication factors by E2F in cancer and embryonic stem cells" in "Control of Cellular Physiology by E2F Transcription Factors" (2008)
  • Author(s): Fujii-Yamamoto, H., Yamada, M., and Masai, H.
  • Total Pages: 12
  • Publisher: Research Signpost
• [Book] 「染色体サイクル:複製・分配組換え・修復・クロマチン制御のメカニズムとその異常による疾患」序文実験医学増刊号 (2007)
  • Author(s): 正井久雄
  • Publisher: 羊土社
• [Book] 概論"染色体サイクル制御の分子メカニズム"実験医学増刊号「染色体サイクル」 (2007)
  • Author(s): 正井久雄、渡邊嘉典
  • Publisher: 羊土社
• [Book] MCMタンパク質のDNA複製における役割"実験医学増刊号「染色体サイクル」 (2007)
  • Author(s): YouZhiying, 正井久雄
  • Publisher: 羊土社
• [Book] Cdc7キナーゼによる複製フォーク制御"実験医学増刊号「染色体サイクル」 (2007)
  • Author(s): 正井久雄、松本清治
  • Publisher: 羊土社
• [Book] 生化学辞典(分担)第4版東京化学同人他12報 (2007)
  • Author(s): 正井久雄
• [Book] 「染色体サイクル:複製・分配・組換え・修復・クロマチン制御のメカニズムとその異常による疾患」序文 実験医学増刊号 (2007)
  • Author(s): 正井 久雄
  • Total Pages: 1
  • Publisher: 羊土社
• [Book] 概論"染色体サイクル制御の分子メカニズム"実験医学増刊号「染色体サイクル」 (2007)
  • Author(s): 正井 久雄、渡邊 嘉典
  • Total Pages: 8
  • Publisher: 羊土社
• [Book] "MCMタンパク質のDNA複製における役割"実験医学増刊号「染色体サイクル」 (2007)
  • Author(s): You Zhiying、正井 久雄
  • Total Pages: 5
  • Publisher: 羊土社
• [Book] "Cdc7キナーゼによる複製フォーク制御"実験医学増刊号「染色体サイクル」 (2007)
  • Author(s): 正井 久雄、松本 清治
  • Total Pages: 8
  • Publisher: 羊土社
• [Book] 生化学辞典(分担)第4版 (2007)
  • Author(s): 正井 久雄
  • Publisher: 東京化学同人
• [Book] "Southwestern法"「分子間相互作用解析ハンドブック」 (2007)
  • Author(s): 田中 卓、正井 久雄
  • Total Pages: 4
  • Publisher: 羊土社
• [Book] "UVクロスリンキング法"「分子間相互作用解析ハンドブック」 (2007)
  • Author(s): You Zhiying, 正井 久雄
  • Total Pages: 4
  • Publisher: 羊土社
• [Book] 染色体サイクル : 複製・分配・組換え・修復・クロマチン制御のメカニズムとその異常による疾患序文 実験医学 増刊号 染色体サイクル (2007)
  • Author(s): 正井久雄
  • Total Pages: 219
  • Publisher: 羊土社
• [Book] 概論"染色体サイクル制御の分子メカニズム"実験医学 増刊号「染色体サイクル」 (2007)
  • Author(s): 正井久雄, 渡邊嘉典
  • Total Pages: 219
  • Publisher: 羊土社
• [Book] "MCMタンパク質のDNAZ複製における役割"実験医学 増刊号「染色体サイクル」 (2007)
  • Author(s): Zhiying You, 正井久雄
  • Total Pages: 219
  • Publisher: 羊土社
• [Book] Cdc7キナーゼによる複製フォーク制御"実験医学増刊号「染色体サイクル」 (2007)
  • Author(s): 正井久雄, 松本清治
  • Total Pages: 219
  • Publisher: 羊土社
• [Book] ヒトの分子生物学(村松正實 監訳) (2006)
  • Author(s): 正井久雄(翻訳分担)
  • Total Pages: 715
  • Publisher: 丸善株式会社
• [Book] 体細胞分裂に重要な役割を果たす分裂酵母Cdc7類似キナーゼ, Hsk1タンパク質は減数分裂期組換えの開始に必要とされる (細胞工学) (2006)
  • Author(s): 荻野桂子, 廣田耕志, 太田邦史, 正井久雄
  • Publisher: 秀潤社
• [Remarks] ホームページ等
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