The roles of phosohoinositides in transportsome

• Project/Area Number: 17081004
• Research Category: Grant-in-Aid for Scientific Research on Priority Areas
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: The University of Tokyo
• Principal Investigator: SUETSUGU Shiro The University of Tokyo, 分子細胞生物学研究所, 准教授 (70345031)
• Co-Investigator(Kenkyū-buntansha): TADAOMI Takenawa 東京大学, 医科学研究所, 教授 (40101315)
• Project Period (FY): 2005 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥59,800,000 (Direct Cost: ¥59,800,000); Fiscal Year 2009: ¥13,200,000 (Direct Cost: ¥13,200,000); Fiscal Year 2008: ¥13,200,000 (Direct Cost: ¥13,200,000); Fiscal Year 2007: ¥13,200,000 (Direct Cost: ¥13,200,000); Fiscal Year 2006: ¥13,200,000 (Direct Cost: ¥13,200,000); Fiscal Year 2005: ¥7,000,000 (Direct Cost: ¥7,000,000)
• Keywords: 細胞膜 / 細胞骨格 / トランスポート / ホスホイノシタイド / エンドサイトーシス / N-WASP / EFCドメイン / 膜変形 / アクチン重合 / SKIP / アクチン / ダイナミン / 小胞形成

Research Abstract

The diverse members of the BAR domain superfamily proteins have structurally determined positive and negative curvatures of membrane contact at their BAR, F-BAR, and I-BAR domains, which are thought to generate and maintain such curved membranes by binding to the membrane of various subcellular structures. Phosphoinositide metabolism is important for regulation of transporters and channels. The BAR domain superfamily proteins bind to the phosphoinositides at the membrane. Importantly, the SH3 domains of the BAR domain superfamily proteins bind to the actin regulatory WASP/WAVE proteins and various proteins including transporters and channels as well as the regulatory proteins of transporters and channels.

Research Products

• [Journal Article] Essential Role of Pacsin2/Syndapin II in Membrane Sculpting for Caveolae (2011)
  • Author(s): Senju, Y., Itoh, Y., Takano, K., Hamada, S., Suetsugu, S.
  • Journal Title: J Cell Sci in press
• [Journal Article] Nebulin and N-WASP Cooperate to Cause IGF-1-induced Sarcomeric Actin Filament Formation. (2010)
  • Author(s): Takano, K., Watanabe-Takano, H., Suetsugu, S., Kurita, S., Tsujita, K., Kimura, S., Karatsu, T., Takenawa, T., Endo, T.
  • Journal Title: Science 330 Pages: 1536-1540
• [Journal Article] The proposed functions of membrane curvatures mediated by the BAR domain superfamily proteins. (2010)
  • Author(s): Suetsugu, S.
  • Journal Title: J Biochem 148 Pages: 1-12
  • NAID: 10027876795
• [Journal Article] Subcellular membrane curvature mediated by the BAR domain superfamily proteins. (2010)
  • Author(s): Suetsugu, S., Toyooka, K., Senju, Y.
  • Journal Title: Semin Cell Dev Biol 21 Pages: 340-349
• [Journal Article] Mapping of the basic amino-acid residues responsible for tubulation and cellular protrusion by the EFC/F-BAR domain of pacsin2/Syndapin II. (2010)
  • Author(s): Shimada, A., Takano, K., Shirouzu, M., Hanawa-Suetsugu, K., Terada, T., Toyooka, K., Umehara, T., Yamamoto, M., Yokoyama, S., Suetsugu, S.
  • Journal Title: FEBS Lett 584 Pages: 1111-1118
• [Journal Article] Mapping of the basic amino-acid residues responsible for tubulation and cellular protrusion by the EFC/F-BAR domain of pacsin2/Syndapin II (2010)
  • Author(s): Shimada A, Takano K, Shirouzu M, Hanawa-Suetsugu K, Terada T, Toyooka K, Umehara T, Yamamoto
  • Journal Title: FEBS Lett 584 Pages: 1111-8
  • Peer Reviewed
• [Journal Article] Subcellular membrane curvature mediated by the BAR domain superfamily proteins (2010)
  • Author(s): Suetsugu S, Toyooka K, Senju Y.
  • Journal Title: Semin Cell Dev Biol. 21 Pages: 340-9
  • Peer Reviewed
• [Journal Article] Mammalian formin fhod3 regulates actin assembly and sarcomere organization in striated muscles. (2009)
  • Author(s): Taniguchi, K., Takeya, R., Suetsugu, S., Kan, O.M., Narusawa, M., Shiose, A., Tominaga, R., Sumimoto, H.
  • Journal Title: J Biol Chem 284 Pages: 29873-29881
• [Journal Article] The direction of actin polymerization for vesicle fission suggested from membranes tubulated by the EFC/F-BAR domain protein FBP17. (2009)
  • Author(s): Suetsugu, S.
  • Journal Title: FEBS Lett 583 Pages: 3401-3404
• [Journal Article] The direction of actin polymerization for vesicle fission suggested from membranes tubulated by the EFC/F-BAR domain protein FBP17 (2009)
  • Author(s): Suetsugu S.
  • Journal Title: FEBS Lett. 284 Pages: 29873-81
  • Peer Reviewed
• [Journal Article] Mammalian formin fhod3 regulates actin assembly and sarcomere organization in striated muscles (2009)
  • Author(s): Taniguchi K, Takeya R, Suetsugu S, Kan-O M, Narusawa M, Shiose A, Tominaga R, Sumimoto H.
  • Journal Title: J Biol Chem. 284 Pages: 29873-81
  • Peer Reviewed
• [Journal Article] G-actin regulates rapid induction of actin nucleation by mDia1 to restore cellular actin polymers. (2008)
  • Author(s): Higashida, C., Suetsugu, S., Tsuji, T., Monypenny, J., Narumiya, S., Watanabe, N.
  • Journal Title: J Cell Sci 121 Pages: 3403-3412
• [Journal Article] EFC/F-BAR proteins and the N-WASP-WIP complex induce membrane-curvature dependent actin polymerization. (2008)
  • Author(s): Takano, K., Toyooka, K., Suetsugu, S.
  • Journal Title: EMBO J 27 Pages: 2817-2828
• [Journal Article] Increased insulin action in SKIP heterozygous knockout mice. (2008)
  • Author(s): Ijuin T, Yu YE, Mizutani K, Pao A, Tateya S, Tamori Y, Bradley A, Takenawa T.
  • Journal Title: Mol Cell Biol. 28(17)(Epub 2008 Jun 23) Pages: 5184-5195
• [Journal Article] IRSp53 : crossing the road of membrane and actin dynamics in the formation of membrane protrusions. (2008)
  • Author(s): Scita, G., Confalonieri, S., Lappalainen, P., Suetsugu, S.
  • Journal Title: Trends Cell Biol 18(2) Pages: 52-60
• [Journal Article] EFC/F-BAR proteins and the NWASP-WIP complex induce membranecurvature dependent actin polymerization (2008)
  • Author(s): Takano, K., Toyooka, K.,Suetsugu, S
  • Journal Title: EMBO Journal 27 Pages: 2817-28
  • Peer Reviewed
• [Journal Article] G-actin regulates rapid induction of actin nucleation by mDial to restore cellular actin polymers (2008)
  • Author(s): Higashida, C., Suetsugu, S., Tsuji, T., Monypenny, J., Narumiya, S., Watanabe. N
  • Journal Title: Journal of Cell Science 121 Pages: 3403-12
  • Peer Reviewed
• [Journal Article] IRSp53: crossing the road of membrane and actin dynamics in the formation of membrane protrusions. (2008)
  • Author(s): Scita, G., Confalonieri, S., Lappalainen, P. & Suetsugu, S.
  • Journal Title: Trends Cell Biol. 18 Pages: 52-60
  • Peer Reviewed
• [Journal Article] SGIP1alpha is an endocytic protein that directly interacts with phospholipids and Eps15. (2007)
  • Author(s): Uezu A, Horiuchi A, Kanda K, Kikuchi N, Umeda K, Tsujita K, Suetsugu S, Araki N, Yamamoto H, Takenawa T, Nakanishi H.
  • Journal Title: J Biol Chem. 282 Pages: 26481-26489
• [Journal Article] Synaptopodin Protects Against Proteinuria by Disrupting Cdc42 : IRSp53 : Mena Signaling Complexes in Kidney Podocytes. (2007)
  • Author(s): Yanagida-Asanuma E, Asanuma K, Kim K, Donnelly M, Young Choi H, Hyung Chang J, Suetsugu S, Tomino Y, Takenawa T, Faul C, Mundel P.
  • Journal Title: Am J Pathol. 171 Pages: 415-427
• [Journal Article] Gently-curved EFC-domain dimers are joined end to end into a filament for membrane invagination in endocytosis. (2007)
  • Author(s): Shimada A, Niwa H, Tsujita K, Suetsugu S, Nitta K, Hanawa-Suetsugu K, Akasaka R, Nishino Y, Toyama M, Chen, Liu Z-J, Wang B-C, Yamamoto M, Terada T, Miyazawa A, Shirouzu M, Tanaka A, Sugano S, Nagayama K, Takenawa T, Yokoyama S.
  • Journal Title: Cell 129 Pages: 761-772
• [Journal Article] Effect of WAVE2 phosphorylation on activation of the Arp2/3 complex. (2007)
  • Author(s): Nakanishi O, Suetsugu S, Yamazaki D, Takenawa T.
  • Journal Title: J Biochem (Tokyo) 141(3)(Epub 2007 Jan 3) Pages: 319-325
  • NAID: 10020100487
• [Journal Article] The WASP-WAVE protein network : connecting the membrane to the cytoskeleton. (2007)
  • Author(s): Takenawa T, Suetsugu S.
  • Journal Title: Nature Reviews Mol Cell Biol. 8 Pages: 37-48
• [Journal Article] Previews : Stress-Associated MAP Kinase Fills in the Map of Filamin-Mediated Neuronal Migration. (2007)
  • Author(s): Suetsugu S, Takenawa T.
  • Journal Title: Dev Cell 12 Pages: 3-4
• [Journal Article] SGIP1alpha is an endocytic protein that directly interacts with phospholipids and Eps15. (2007)
  • Author(s): Uezu, A., Horiuchi, A., Kanda, K., Kikuchi, N., Umeda, K., Tsujita, K., Suetsugu, S., Araki, N., Yamamoto, H., Takenawa, T. &Nakanishi, H.
  • Journal Title: J Biol Chem. 282 Pages: 26481-9
  • Peer Reviewed
• [Journal Article] Synaptopodin protects against proteinuria by disrupting Cdc42:IRSp53:Mena signaling complexes in kidney podocytes. (2007)
  • Author(s): Yanagida-Asanuma, E., Asanuma, K., Kim, K., Donnelly, M., Young Choi, H., Hyung Chang, J., Suetsugu, S., Tomino, Y., Takenawa, T., Faul, C. & Mundel. P.
  • Journal Title: Am J Pathol. 171 Pages: 415-27
  • Peer Reviewed
• [Journal Article] Curved EFC/F-BAR-domain dimers are joined end to end intoa filament for membrane invagination in endocytosis. (2007)
  • Author(s): Shimada, A*, Niwa, H.*, Tsujita, K.*, Suetsugu, S.*, Nitta, K., Hanawa-Suetsugu, K., Akasaka, R., Nishino, Y., Toyama, M., Chen, L., Liu, Z.J., Wang, B.C., Yamamoto, M., Terada, T., Miyazawa, A., Tanaka, A., Sugano, S., Shirouzu, M., Nagayama, K., Takenaw, T & Yokoyama, S.
  • Journal Title: Cell. 129 Pages: 761-72
  • Peer Reviewed
• [Journal Article] Effect of WAVE2 Phosphorylation on Activation of the Arp2/3 Complex. (2007)
  • Author(s): Nakanishi O, Suetsugu S, Yamazaki D, Takenawa T.
  • Journal Title: J Biochem (Tokyo). 141・3 Pages: 319-325
  • NAID: 10020100487
• [Journal Article] Stress-associated MAP kinase fills in the map of filamin-mediated neuronal migration. (2007)
  • Author(s): Suetsugu S, Takenawa T.
  • Journal Title: Dev Cell 12・1 Pages: 3-4
• [Journal Article] The WASP-WAVE protein network : connecting the membrane to the cytoskeleton. (2007)
  • Author(s): Takenawa T, Suetsugu S.
  • Journal Title: Nat Rev Mol Cell Biol. 8・1 Pages: 37-48
• [Journal Article] The RAC binding domain/IRSp53-MIM homology domain of IRSp53 induces RAC-dependent membrane deformation. (2006)
  • Author(s): Suetsugu S, Murayama K, Sakamoto A, Hanawa-Suetsugu K, Seto A, Oikawa T, Mishima C, Shirouzu M, Takenawa T, Yokoyama S.
  • Journal Title: J Biol Chem. 281(46)(Epub 2006 Sep 25) Pages: 35347-3558
• [Journal Article] Optimization of WAVE2 complex-induced actin polymerization by membrane-bound IRSp53, PIP(3), and Rac. (2006)
  • Author(s): Suetsugu S, Kurisu S, Oikawa T, Yamazaki D, Oda A, Takenawa T.
  • Journal Title: J Cell Biol. 173(4)(Epub 2006 May 15) Pages: 571-585
• [Journal Article] Protein kinase A-dependent increase in WAVE2 expression induced by the focal adhesion protein vinexin. (2006)
  • Author(s): Mitsushima M, Sezaki T, Akahane R, Ueda K, Suetsugu S, Takenawa T, Kioka N.
  • Journal Title: Genes Cells. 11(3) Pages: 281-292
• [Journal Article] Coordination between the actin cytoskeleton and membrane deformation by a novel membrane tubulation domain of PCH proteins is involved in endocytosis. (2006)
  • Author(s): Tsujita K, Suetsugu S, Sasaki N, Furutani M, Oikawa T, Takenawa T.
  • Journal Title: J Cell Biol. 172(2) Pages: 269-279
• [Journal Article] The RAC binding domain/IRSp53-MIM homology domain of IRSp53 induces RAC-dependent membrane deformation. (2006)
  • Author(s): Suetsugu S, Murayama K, Sakamoto A, Hanawa-Suetsugu K, Seto A, Oikawa T, Mishima C, Shirouzu M, Takenawa T, Yokoyama S.
  • Journal Title: J Biol Chem. 281・46 Pages: 35347-35358
• [Journal Article] Optimization of WAVE2 complex-induced actin polymerization by membrane-bound IRSp53, PIP(3), and Rac. (2006)
  • Author(s): Suetsugu S, Kurisu S, Oikawa T, Yamazaki D, Oda A, Takenawa T.
  • Journal Title: J Cell Biol. 173・4 Pages: 571-585
• [Journal Article] Optimization of WAVE2-complex-induced actin polymerization by membrane-bound IRSp53, PIP3, and Rac. (2006)
  • Author(s): Suetsugu S, Kurisu S, Yamazaki D, Oikawa T, Oda A, Takenawa T.
  • Journal Title: J Cell Biol. in press
• [Journal Article] Protein kinase A-dependent increase in WAVE2 expression induced by the focal adhesion protein vinexin. (2006)
  • Author(s): Mitsushima M, Sezaki T, Akahane R, Ueda K, Suetsugu S, Takenawa T, Kioka N.
  • Journal Title: Genes Cells. 11・3 Pages: 281-292
• [Journal Article] Coordination between the actin cytoskeleton and membrane deformation by a novel membrane tubulation domain of PCH proteins is involved in endocytosis. (2006)
  • Author(s): Tsujita K, Suetsugu S, Sasaki N, Furutani M, Oikawa T, Takenawa T.
  • Journal Title: J Cell Biol 172・2 Pages: 269-279
• [Journal Article] WAVE3 functions as a negative regulator of LDOC1. (2005)
  • Author(s): Mizutani K, Koike D, Suetsugu S, Takenawa T.
  • Journal Title: J Biochem (Tokyo) 138(5) Pages: 639-646
  • NAID: 10017348480
• [Journal Article] A novel function of WAVE in lamellipodia : WAVE1 is required for stabilization of lamellipodial protrusions during cell spreading. (2005)
  • Author(s): Yamazaki D, Fujiwara T, Suetsugu S, Takenawa T
  • Journal Title: Genes Cells. 10(5) Pages: 381-392
• [Journal Article] Interaction of HSP90 to N-WASP leads to activation and protection from proteasome-dependent degradation. (2005)
  • Author(s): Park, S.J., S.Suetsugu, T.Takenawa
  • Journal Title: Embo J 24(8) Pages: 1557-1570
• [Journal Article] WAVE3 functions as a negative regulator of LDOC1 (2005)
  • Author(s): Mizutani K, Koike D, Suetsugu S, Takenawa T.
  • Journal Title: J Biochem (Tokyo) 138・5 Pages: 639-646
  • NAID: 10017348480
• [Journal Article] A novel function of WAVE in lamellipodia : WAVE1 is required for stabilization of lamellipodial protrusions during cell spreading. (2005)
  • Author(s): Yamazaki D, Fujiwara T, Suetsugu S, Takenawa T.
  • Journal Title: Genes Cells. 10・5 Pages: 381-392
• [Journal Article] Interaction of HSP90 to N-WASP leads to activation and protection from proteasome-dependent degradation. (2005)
  • Author(s): Park SJ, Suetsugu S, Takenawa T.
  • Journal Title: EMBO J. 24・8 Pages: 1557-1570
• [Presentation] EFC/F-BARドメインタンパク質FBP17およびToca-1とN-WASP-WIP複合体による膜曲率依存的アクチン重合の誘導 (2009)
  • Organizer: 第31回分子生物学会年会第81回日本生化学会大会合同大会(BMB2008)
  • Place of Presentation: 神戸国際会議場
  • Year and Date: 2009-12-10
• [Presentation] Membrane tubulation and actin polymerization induced by the EFC/F-BAR domain protein pacsin2 for caveolae formation (2009)
  • Organizer: the 49th Annual Meeting of the American Society for Cell Biology
  • Place of Presentation: San Diego
  • Year and Date: 2009-12-07
• [Presentation] Membrane tubulation and actin polymerization induced by the EFC/F-BAR domain protein pacsin2 for caveolae formation (2009)
  • Author(s): 末次志郎
  • Organizer: 2009 ASCB Annual Meeting
  • Place of Presentation: San Diego Convention Center USA
  • Year and Date: 2009-12-07
• [Presentation] The EFC/F-BAR protein that is involved in caveolae formation (2009)
  • Organizer: The second conference on F-BAR proteins
  • Place of Presentation: ストックホルム
  • Year and Date: 2009-10-02
• [Presentation] The EFC/F-BAR protein that is involved in caveolae formation (2009)
  • Author(s): 末次志郎
  • Organizer: The second conference on F-BAR proteins
  • Place of Presentation: Ranas Alott(ヘルシンキ)フィンランビ
  • Year and Date: 2009-10-01
• [Presentation] Proteins that remodel the membrane shape and their physiological functions. (2009)
  • Organizer: IUPS2009
  • Place of Presentation: 京都国際会議場
  • Year and Date: 2009-07-31
• [Presentation] 膜曲率によるアクチン重合の制御 (2009)
  • Organizer: 第60回日本細胞生物学会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2009-07-01
• [Presentation] EFC/F-BAR domain super family (2009)
  • Organizer: 第60回日本細胞生物学会
  • Place of Presentation: 名古屋国際会議場
  • Year and Date: 2009-06-03
• [Presentation] EFC/F-BARドメインスーパーファミリーの細胞膜局在 (2009)
  • Author(s): 末次志郎
  • Organizer: 第61回日本細胞生物学会
  • Place of Presentation: 名古屋国際会議場
  • Year and Date: 2009-06-03
• [Presentation] EFC/F-BAR proteins and the N-WASP-WIP complex bind to membranes to induce membrane-curvature dependent actin polymerization. (2008)
  • Organizer: the 48th Annual Meeting of the American Society for Cell Biology Moscone Center
  • Place of Presentation: San Francisco
  • Year and Date: 2008-12-17
• [Presentation] EFC/F-BAR proteins and the N-WASP-WIP complex bind to membranes to induce membranecurvature dependent actin polymerizatio (2008)
  • Author(s): Suetsugu, Shiro
  • Organizer: the 48th Annual Meeting of the American Society for Cell Biology
  • Place of Presentation: Moscone Center(San Francisco)
  • Year and Date: 2008-12-17
• [Presentation] EFC/F-BARドメインタンパク質FBP17およびToca-1とN-WASP-WIP複合体による膜曲率依存的アクチン重合の誘導 (2008)
  • Author(s): 末次志郎
  • Organizer: 第31回分子細胞生物学会年会第81回日本生化学会大会合同大会(BMB2008)
  • Place of Presentation: 神戸国際会議場
  • Year and Date: 2008-12-10
• [Presentation] 膜曲率によるアクチン重合の制御 (2008)
  • Author(s): 末次志郎
  • Organizer: 第60回日本細胞生物学会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2008-07-01
• [Presentation] The rearrangemrnts of actin cytoskeleton dependent on the shapes of the plasma membrane. (2007)
  • Organizer: 第30回日本分子生物学会・第80回日本生化学会大会合同大会
  • Place of Presentation: 横浜インターコンチネンタルホテル
  • Year and Date: 2007-12-12
• [Presentation] The rearrangements of actin cytoskeleton dependent on the shapes of the plasma membrane. (2007)
  • Author(s): 末次 志郎
  • Organizer: 第30回日本分子生物学会年会・第80回日本生化学会大会合同大会
  • Place of Presentation: インターコンチネンタルホテル
  • Year and Date: 2007-12-12
• [Presentation] Control of Cell Shape and Motility : new approaches to understand its mechanistic basis. (2007)
  • Organizer: 第40回日本発生生物学会・第59回日本細胞生物学会合同大会
  • Place of Presentation: 福岡国際会議場
  • Year and Date: 2007-05-30
• [Presentation] Control of Cell Shape and Motility: new approaches to understand itsmechanistic basis (2007)
  • Author(s): 末次 志郎
  • Organizer: 第40回日本発生生物学会・第59回日本細胞生物学会合同大会
  • Place of Presentation: 福岡国際会議場
  • Year and Date: 2007-05-30
• [Presentation] Connectig the membrane to the actin cytoskeleton by the BAR, EFC, and RCB domain proteins and WASP/WAVE proteins. (2007)
  • Organizer: Workshop on Cell Migration IFOM-IEO Campus
  • Place of Presentation: 伊:ミラノ
  • Year and Date: 2007-05-12
• [Presentation] Connecting the membrane to the actin cytoskeleton by BAR, EFC, and RCB domain proteins and WASP/WAVE proteins (2007)
  • Author(s): 末次 志郎
  • Organizer: Workshop on Cell Migration
  • Place of Presentation: IFOM-IEO Campus in Milan
  • Year and Date: 2007-05-12
• [Presentation] 2006フォーラム (2006)
  • Organizer: 日本分子生物学会
  • Place of Presentation: 名古屋国際会議場
• [Presentation] (2005)
  • Organizer: 第28回日本分子生物学会
  • Place of Presentation: 福岡国際会議場
• [Presentation] (2005)
  • Organizer: 第78回日本生化学会大会
  • Place of Presentation: 神戸国際会議場
• [Remarks]
• [Remarks]
  • URL: http://www.suetsugulab.net/en/Publications.html
• [Remarks]
  • URL: http://www.iam.u-tokyo.acjplsuetsugu/