Identification of renal transportsomes by analyzing disease-causing mutants of transporters and their regulators.
| Project/Area Number |
17081009
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| Research Category |
Grant-in-Aid for Scientific Research on Priority Areas
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| Allocation Type | Single-year Grants |
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| Review Section |
Biological Sciences
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| Research Institution | Tokyo Medical and Dental University |
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Principal Investigator |
UCHIDA Shinichi Tokyo Medical and Dental University, 大学院・医歯学総合研究科, 准教授 (50262184)
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| Co-Investigator(Kenkyū-buntansha) |
SASAKI Sei 東京医科歯科大学, 医歯学総合研究科, 准教授 (60170677)
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| Project Period (FY) |
2005 – 2009
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| Project Status |
Completed (Fiscal Year 2009)
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| Budget Amount *help |
¥71,100,000 (Direct Cost: ¥71,100,000)
Fiscal Year 2009: ¥13,300,000 (Direct Cost: ¥13,300,000)
Fiscal Year 2008: ¥15,100,000 (Direct Cost: ¥15,100,000)
Fiscal Year 2007: ¥17,800,000 (Direct Cost: ¥17,800,000)
Fiscal Year 2006: ¥17,800,000 (Direct Cost: ¥17,800,000)
Fiscal Year 2005: ¥7,100,000 (Direct Cost: ¥7,100,000)
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| Keywords | 水チャネル / クロライドチャネル / WNKキナーゼ / AQP水チャネル / CLCクロライドチャネル / 塩分感受性高血圧 / アルドステロン / 遺伝子改変マウス / AKAP / 酵母two-hybrid法 / 高血圧 / 膜輸送体 / 遺伝子組み換えマウス / 偽性低アルドステロン症II型 / ノックインマウス / claudin / Na-Cl共輸送体 / 尿崩症 / 蛋白複合体 / 細胞内局在 |
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| Research Abstract |
The aim of this study was to identify functionally important proteincomplexes regulating AQP water channels, CLC chloride channels and Na-Cl cotransporter(NCC), by focusing on the naturally occurring mutations of these genes. We found that thebinding of tropomyosin 5b to phosphorylated AQP2 was necessary for AQP2's apical sortingby vasopressin. We also found a novel phosphorylation cascade (WNK-OSR1/SPAK-NCC)in the kidney that is important for blood pressure regulation.
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Report
(6 results)
Research Products
(69 results)
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[Journal Article] Pancreas-specific aquaporin 12 null mice showed increased susceptibility to caerulein-induced acute pancreatitis.2009
Author(s)
Ohta E, Itoh T, Nemoto T, Kumagai J, Ko SB, Ishibashi K, Ohno M, Uchida K, Ohta A, Sohara E, Uchida S, Sasaki S, Rai T.
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Journal Title
Am. J. Physiol. Cell Physiol. 297
Pages: 1368-78
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[Journal Article] Reciprocal interaction with G-actin and tropomyosin is essential for aquaporin-2 trafficking.2008
Author(s)
Noda Y, Horikawa S, Kanda E, Yamashita M, Meng H, Eto E, Li Y, Kuwahara M, Hirai K, Pack C, Kinjo M, Okabe S, Sasaki S.
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Journal Title
J. Cell Biol. 182
Pages: 587-601
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[Journal Article] Molecular pathogenesis of pseudohypoaldosteronism type II: generation and analysis of a Wnk4 D561A/+ knock-in mouse model.2007
Author(s)
Yang SS, Morimoto T, Rai T, Chiga M, Sohara E, Ohno M, Uchida K, Lin S-H, Moriguchi T, Shibuya H, Kondo Y, Sasaki S, Uchida S.
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Journal Title
Cell Metab 5
Pages: 331-344
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[Journal Article] Disruption of aquaporin-11 produces polycystic kidneys following vacuolization of the proximal tubule.2005
Author(s)
Morishita Y, Matsuzaki T, Hara-Chikuma M, Ando A, Shimono M, Matsuki A, Kobayashi K, Ikeda M, Yamamoto T, Verkman A, Kusano E, Ookawara S, Takata K, Sasaki S, Ishibashi K.
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Journal Title
Mol Cell Biol. 25
Pages: 7770-7779
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[Presentation] Molecular pathogenesis of Bartter syndrome caused by R8L barttin mutation.2009
Author(s)
Uchida S, Nomura N, Sugawara N, Morimoto T, Kondo Y, Naito S, Yui N, Oi K, Talat G, KMZ Hossain, Wakabayashi M, Nishida H, Ohta E, Ohta A, Sohara E, Rai T, Sasaki S.
Organizer
40th NIPS International Symposium & Physiology of anion transport and cell volume regulation (PAT-CVR 2009)
Place of Presentation
Okazaki
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[Presentation] Generation and analysis of Wnk4D561A/+ knock-in mice.2007
Author(s)
Yang S, Morimoto T, Rai T, Chiga M, Sohara E, Ohno M, Uchida K, Lin S, Moriguchi T, Shibuya H, Kondo Y, Sasaki S, Uchida S.
Organizer
ISN- Nature Genetics Forefronts Symposium on Nephrogenetics: from Development to Physiology
Place of Presentation
Danvers, USA
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