Single-molecule tracking study of the formation and functions of raft molecular complexes

• Project/Area Number: 17081010
• Research Category: Grant-in-Aid for Scientific Research on Priority Areas
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: Kyoto University
• Principal Investigator: KUSUMI Akihiro Kyoto University, 物質-細胞統合システム拠点, 教授 (50169992)
• Project Period (FY): 2005 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥43,500,000 (Direct Cost: ¥43,500,000); Fiscal Year 2009: ¥9,300,000 (Direct Cost: ¥9,300,000); Fiscal Year 2008: ¥9,300,000 (Direct Cost: ¥9,300,000); Fiscal Year 2007: ¥9,300,000 (Direct Cost: ¥9,300,000); Fiscal Year 2006: ¥10,800,000 (Direct Cost: ¥10,800,000); Fiscal Year 2005: ¥4,800,000 (Direct Cost: ¥4,800,000)
• Keywords: IgE-Fc受容体 / 可塑的ナノドメイン / 細胞膜 / 1分子追跡・1分子イメジング / 可塑的分子複合体 / ナノドメイン / 蛍光顕微鏡

Research Abstract

Intracellular lipid-anchored signaling molecules, Gαi2, Lyn, and PLCγ, were found to be transiently recruited to ligand-induced CD59-cluster rafts, leading to intracellular IP3-calcium signaling.

Research Products

• [Journal Article] Hierarchical organization of the plasma membrane : Investigations by single-molecule tracking vs. fluorescence correlation spectroscopy (2010)
  • Author(s): A.Kusumi, Y.M.Shirai, I.Koyama-Honda, K.G.Suzuki, T.K.Fujiwara
  • Journal Title: FEBS Letters 584 Pages: 1814-1823
  • Peer Reviewed
• [Journal Article] カラム「ランダムな運動に法則を見出す」 (2009)
  • Author(s): 楠見明、藤原敬宏
  • Journal Title: 「math stories変化をとらえる」高橋陽一郎著東京図書 Pages: 238-247
• [Journal Article] Both MHC class II and its GPI-anchored form undergo hop diffusion as observed by single-molecule tracking (2008)
  • Author(s): Y.M. Umemura, M. Vrljic, S.Y. Nishimura, T.K. Fujiwara, K.G.N. Suzuki, A. Kusumi
  • Journal Title: Biophysical Journal Vol.95 Pages: 435-450
  • Peer Reviewed
• [Journal Article] Both MHC class II and its GPI-anchored form undergo hop diffusion as observed by single-molecule tracking (2008)
  • Author(s): Y. M. Umemura, M. Vrljic, S. Y. Nishimura, TK. Fujiwara, KG. Suzuki, and A. Kusumi
  • Journal Title: Biophys. J 95 Pages: 435-450
  • Peer Reviewed
• [Journal Article] Three-dimensional molecular architecture of the plasma-membrane-associated cytoskeleton as reconstructed by freeze-etch electron tomography (2008)
  • Author(s): N. Morone, C. Nakada, Y. Umemura, J. Usukura, and A. Kusumi
  • Journal Title: Methods in Cell Biology : Introduction to Electron Microscopy for Biologists, T. Allen Ed. Chapter 12 88 Pages: 207-236
  • Peer Reviewed
• [Journal Article] Paradigm shift of the molecular dynamics concept in the cell membrane : high-speed single-molecule tracking revealed the partitioning of the cell membrane (2008)
  • Author(s): A. Kusumi, Y. Umemura, N. Morone, and T. Fujiwara
  • Journal Title: Anomalous Transport : Foundations and Applications, R. Klages Ed. Chapter 19, Wiley Interscience Pages: 545-574
  • Peer Reviewed
• [Journal Article] Mechanism for signal transduction in the induced-raft domains as revealed by single molecule tracking (2008)
  • Author(s): K. G. N. Suzuki, and A. Kusumi
  • Journal Title: Trends in Glycoscience and Glycotechnology 20(116) Pages: 341-351
  • NAID: 10026975802
  • Peer Reviewed
• [Journal Article] ディジタルシグナル変換場としてはたらくon-demandラフトドメイン (2008)
  • Author(s): 楠見明弘、鈴木健一
  • Journal Title: 膜(MEMBRANE) 33(5) Pages: 194-200
  • NAID: 10026876395
  • Peer Reviewed
• [Journal Article] ディジタルシグナル変換場としてはたらく誘導ラフトドメイン (2008)
  • Author(s): 鈴木健一、楠見明弘
  • Journal Title: 生物物理 48(6) Pages: 320-324
  • NAID: 110007005778
  • Peer Reviewed
• [Journal Article] 1分子追跡でみえてきた細胞膜ラフトが働くしくみ (2008)
  • Author(s): 鈴木健一、楠見明弘
  • Journal Title: 実験医学増刊号「生命現象の動的理解を目指すライプイメージング」12 Pages: 96-103
  • Peer Reviewed
• [Journal Article] Both MHC class II and its GPI-anchored form undergo hopdiffusion as observed by single-molecule tracking (2008)
  • Author(s): Y. M. Umemura, M. Vrljic, S. Y. Nishimura, T. K. Fujiwara, K. G. N. Suzuki, and A. Kusumi
  • Journal Title: Biophys. J. (印刷中)(印刷中)
  • Peer Reviewed
• [Journal Article] Dynamic recruitment of phospholipase Cγat transiently immobilized GPI-anchored receptor clusters induces IP3-Ca2+ signaling: single-molecule tracking study 2 (2007)
  • Author(s): K.G.N. Suzuki, T.K. Fujiwara, M. Edidin, A. Kusumi
  • Journal Title: Journal of Cell Biology Vol.177 Pages: 731-742
  • Peer Reviewed
• [Journal Article] GPI-anchored receptor clusters transiently recruit Lyn and Ga for temporary cluster immobilization and Lyn activation: single-molecule tracking study 1 (2007)
  • Author(s): K.G.N. Suzuki, T.K. Fujiwara, F. Sanematsu, R. Iino, M. Edidin, A. Kusumi
  • Journal Title: Journal of Cell Biology Vol.177 Pages: 717-730
  • Peer Reviewed
• [Journal Article] GPI-anchored receptor clusters transiently recruit Lyn and Ga for temporary cluster immobilization and Lyn activation: single-molecule tracking study l (2007)
  • Author(s): K. G. N. Suzuki. T. K. Fujiwara, F. Sanematsu, R. Iino, M. Edidin, and A. Kusumi.
  • Journal Title: J. Cell Biol. 177 Pages: 717-730
  • Peer Reviewed
• [Journal Article] Dynamic recruitment of phospholipase Cg at transiently immobilized GPI-anchored receptorclusters induces IP3-Ca2+ signaling: single-molecule tracking study 2. (2007)
  • Author(s): K. G. N. Suzuki, T. K. Fujiwara, M. Edidin, and A. Kusumi.
  • Journal Title: J. Cell Biol. 177 Pages: 731-742
  • Peer Reviewed
• [Journal Article] Three-dimensional reconstruction of the membrane skeleton at the plasma membrane interface by electron tomography (2006)
  • Author(s): N. Morone, T. Fujiwara, K. Murase, R.S. Kasai, H. Ike, S. Yuasa, J. Usukura, A. Kusumi
  • Journal Title: Journal of Cell Biology Vol.174 Pages: 851-862
  • Peer Reviewed
• [Journal Article] Three-dimensional reconstruction of the membrane skeleton at the plasma membrane interface by electron tomography (2006)
  • Author(s): N.Morone, T.Fujiwara, K.Murase, R.S.Kasai, H.Ike, S.Yuasa, J.Usukura, A.Kusumi
  • Journal Title: J.Cell Biol. 174・6 Pages: 854-862
• [Journal Article] Three-dimensional dynamic structure of the liquid-ordered domain in lipid membranes as examined by pulse-EPR oxygen probing (2006)
  • Author(s): WK.Subczynski, A.Wisniewska, JS.Hyde, A.Kusumi
  • Journal Title: Biophys.J. 92・5 Pages: 1573-1584
• [Journal Article] Fluorescence imaging for monitoring the colocalization of two single molecules in living cells (2005)
  • Author(s): I. Koyama-Honda, K. Ritchie, T. Fujiwara, R. Iino, H. Murakoshi, R.S. Kasai, A. Kusumi
  • Journal Title: Biophysical Journal Vol.88 Pages: 2126-2136
  • Peer Reviewed
• [Journal Article] Detection of non-Brownian diffusion in the cell membrane in single molecule tracking (2005)
  • Author(s): K.Ritchie, †X.-Y.Shan, J.Kondo, K.Iwasawa, K.Fujiwara, A.Kusumi
  • Journal Title: Biophysical Journal 88 Pages: 2266-2277
• [Journal Article] Fluorescence imaging for monitoring the colocalization of two single molecules in living cells (2005)
  • Author(s): I.Koyama-Honda, †K.Ritchie, T.Fujiwara, †R.Iino, †H.Murakoshi, R.Kasai, A.Kusumi
  • Journal Title: Biophysical Journal 88 Pages: 2126-2136
• [Journal Article] Rapid hop diffusion of a G-protein-coupled receptor in the plasma membrane as revealed by single-molecule technique (2005)
  • Author(s): K.Suzuki, †K.Ritchie, †E.Kajikawa, T.Fujiwara, A.Kusumi
  • Journal Title: Biophysical Journal 88 Pages: 3659-3680
• [Presentation] 1分子追跡による細胞膜中でのABCA1動態の解明 (2010)
  • Author(s): 永田紅、沖田千枝子、楠見明弘、植田和光
  • Organizer: 日本農芸化学会2010年度大会
  • Place of Presentation: 東京
  • Year and Date: 2010-03-28
• [Presentation] 細胞膜メゾドメインの動的構造・機能を1分子で見る (2010)
  • Author(s): 楠見明弘
  • Organizer: 日本物理学会第65回年次大会
  • Place of Presentation: 岡山
  • Year and Date: 2010-03-20
• [Presentation] 細胞膜の準安定メゾスコピック動的複合体の機能:1分子イメージングによる研究 (2010)
  • Author(s): 楠見明弘
  • Organizer: 特定領域研究公開シンポジウム「生体膜トランスポートソームの分子構築と生理機能」
  • Place of Presentation: 吹田
  • Year and Date: 2010-03-19
• [Presentation] Raft-based signal transduction based on transient molecular interactions revealed by single-molecule imaging (2010)
  • Author(s): A.Kusumi
  • Organizer: Fifty Years of Biophysics Research at Nagoya University
  • Place of Presentation: 名古屋
  • Year and Date: 2010-03-12
• [Presentation] Signal-molecule tracking approaches to membrane domains (2010)
  • Author(s): A. Kusumi
  • Organizer: HFSP Frontiers Meeting
  • Place of Presentation: ストラスブール(フランス)
  • Year and Date: 2010-03-04
• [Presentation] Signal-molecule tracking approaches to membrane domains (2010)
  • Author(s): A.Kusumi
  • Organizer: HFSP Frontiers Meeting
  • Place of Presentation: ストラスブール、フランス
  • Year and Date: 2010-03-04
• [Presentation] Analysis of ABCA1 dynamics in the plasma membrane by single-molecule tracking (2010)
  • Author(s): K.Nagata, C.Nakada, A.Kusumi, K.Ueda
  • Organizer: 3rd FEBS Special Meeting "ATP-Binding Cassette (ABC) Proteins : From Multidrug Resistance to Genetic Diseases"
  • Place of Presentation: インスブルグ、オーストリア
  • Year and Date: 2010-03-03
• [Presentation] 細胞膜シグナル変換のスイッチ機構:1分子追跡による研究 (2010)
  • Author(s): 楠見明弘
  • Organizer: 法政大学分子ウイルス学研究室主催セミナー
  • Place of Presentation: 東京
  • Year and Date: 2010-03-02
• [Presentation] Direct observation of hop diffusion of lipid and protein molecules in the plasma membrane by high-speed single fluorescent-molecule imaging (2010)
  • Author(s): T.K.Fujiwara, S.Takeuchi, Y.Nagai, K.Hanaka, K.Iwasawa, K.G.N.Suzuki, A.Kusumi
  • Organizer: The 54th Annual Meeting of Biophysical Society of the U.S.A.
  • Place of Presentation: サンフランシスコ、アメリカ
  • Year and Date: 2010-02-22
• [Presentation] Recruitment of NMDA receptor to the synapse by the concerted actions of exocytosis and lateral diffusion in the plasma membrane (2010)
  • Author(s): C.Nakada, Hiroko Hijikata, Yuri L.Nemoto, H.Yoshida, S.Okabe, T.Fujiwara, A.Kusumi
  • Organizer: The 6th iCeMS International Symposium/ The 13th International Membrane Research Forum
  • Place of Presentation: 京都
  • Year and Date: 2010-01-29
• [Presentation] Signal transduction in the plasma membrane as studied by single-molecule tracking (2009)
  • Author(s): A.Kusumi
  • Organizer: Watching Biomolecules in Action 2009
  • Place of Presentation: 豊中
  • Year and Date: 2009-12-16
• [Presentation] 細胞膜シグナル変換のスイッチを可能にする準安定ナノ・メゾドメイン (2009)
  • Author(s): 楠見明弘
  • Organizer: 第25回Wakoワークショップ「細胞膜ナノドメイン:統合的理解と新たな展開」
  • Place of Presentation: 東京
  • Year and Date: 2009-11-17
• [Presentation] Molecular trapped time in metastable raft domains in steady-state cells is much shorter than 1 ms/刺激前細胞でのラフト分子のラフト滞在時間は1ミリ秒よりはるかに短い (2009)
  • Author(s): K.G.N.Suzuki, T.K.Fujiwara, A.Kusumi
  • Organizer: 第47回日本生物物理学会年会口頭発表1TA5「細胞生物的課題(接着、運動、骨格、伝達、膜)」
  • Place of Presentation: 徳島
  • Year and Date: 2009-10-30
• [Presentation] First determination of the dimer dissociation constant of GPCR in the living cell membrane by single-molecule imaging/1分子イメジングによるGPCRダイマーの解離定数の決定 (2009)
  • Author(s): 笠井倫志,E.R.Prossnitz,楠見明弘
  • Organizer: 第47回日本生物物理学会年会口頭発表1TA3「生体膜・人工膜一情報伝達」
  • Place of Presentation: 徳島
  • Year and Date: 2009-10-30
• [Presentation] GPIアンカー型受容体の細胞膜層を介した情報変換:2色1分子追跡による研究 (2009)
  • Author(s): 小山-本田郁子、藤原敬宏、笠井倫志、八原雅子、梶川絵理子、楠見明弘
  • Organizer: 第82回日本生化学会大会
  • Place of Presentation: 神戸
  • Year and Date: 2009-10-24
• [Presentation] 細胞膜シグナル変換の仕組みを1分子追跡で解く (2009)
  • Author(s): 楠見明弘
  • Organizer: 第33回星薬科大学大学院研究科助手会・大学院自治会合同公開セミナー
  • Place of Presentation: 東京
  • Year and Date: 2009-10-10
• [Presentation] ラフト領域の働きを1分子追跡で解く (2009)
  • Author(s): 楠見明弘
  • Organizer: 第29回日本糖質学会年会シンポジウム「生命-分子が語る、細胞が語る」
  • Place of Presentation: 高山
  • Year and Date: 2009-09-11
• [Presentation] Signal transduction of GPI-anchored proteins as studied by single-molecule tracking analysis (2009)
  • Author(s): A. Kusumi
  • Organizer: The EMBO meeting: advancing the life sciences
  • Place of Presentation: アムステルダム(オランダ)
  • Year and Date: 2009-09-01
• [Presentation] Signal transduction of GPI-anchored proteins as studied by single-molecule tracking analysis (2009)
  • Author(s): A.Kusumi
  • Organizer: The EMBO meeting : advancing the life sciences
  • Place of Presentation: アムステルダム、オランダ
  • Year and Date: 2009-09-01
• [Presentation] GPIアンカー型分子のラフト依存短寿命ホモダイマー (2009)
  • Author(s): 楠見明弘
  • Organizer: 文部科学省科学研究費補助金特定領域研究「生体膜トランスポートソームの分子構築と生理機能」平成21年度第1回班会議公開シンポジウム
  • Place of Presentation: 熊本
  • Year and Date: 2009-08-26
• [Presentation] 細胞膜メゾドメインのバイオコントロール (2009)
  • Author(s): 楠見明弘
  • Organizer: 文部科学省科学研究費補助金特定領域研究マルチスケール操作によるシステム細胞工学バイオコントロールシンポジウム
  • Place of Presentation: 東京
  • Year and Date: 2009-08-21
• [Presentation] Mechanism for raft-based signal transduction as studied by single-molecule tracking (2009)
  • Author(s): K.G.N.Suzuki, A.Kusumi
  • Organizer: 5th Leukocyte Signal Transduction Workshop
  • Place of Presentation: クレタ島、ギリシャ
  • Year and Date: 2009-06-17
• [Presentation] Signal transduction via meso-scale raft domains as studied by single-molecule tracking (2009)
  • Author(s): A.Kusumi
  • Organizer: The Fourth iCeMS International Symposium
  • Place of Presentation: 京都
  • Year and Date: 2009-05-27
• [Presentation] 1分子追跡が明らかにする細胞膜ラフトの協同的スイッチ機構 (2009)
  • Author(s): 楠見明弘
  • Organizer: 第114回日本解剖学会総会・全国学術集会シンポジウムS16「新しいマイクロイメージング法を用いた形態形成と遺伝子機能の解析」
  • Place of Presentation: 岡山
  • Year and Date: 2009-03-29
• [Presentation] 1分子追跡が明らかにする細胞膜上のデジタル式情報変換機構 (2009)
  • Author(s): 楠見明弘
  • Organizer: 東北大学理学研究科グローバルCOEプログラム「物質階層を紡ぐ科学フロンティアの新展開」 : 「膜の研究
  • Place of Presentation: 仙台
  • Year and Date: 2009-03-08
• [Presentation] 1分子で見る細胞膜上のシグナル変換 (2009)
  • Author(s): 楠見明弘
  • Organizer: 日本顕微鏡学会第33回関東支部講演会「顕微鏡3Dイメージングの最前線」
  • Place of Presentation: 東京
  • Year and Date: 2009-03-07
• [Presentation] 細胞膜ドメイン構造がはたらく仕組み : 1分子ナノ操作・追跡による研究 (2009)
  • Author(s): 楠見明弘
  • Organizer: 文部科学省科学研究費補助金特定領域研究マルチスケール操作によるシステム細胞工学(バイオ操作)第7回公開シンポジウム
  • Place of Presentation: 仙台
  • Year and Date: 2009-03-06
• [Presentation] Glycosphingolipids and Non-Raft Phospholipids Exhibit Very Similar Dynamics in Single-Molecule Observations (2009)
  • Author(s): K. G. N. Suzuki, T. K. Fujiwara, and A. Kusumi
  • Organizer: The 53rd Annual Meeting of Biophysical Society of the U. S. A.
  • Place of Presentation: ボストン、アメリカ
  • Year and Date: 2009-03-02
• [Presentation] 細胞膜の領域構造が可能にする細胞情報伝達 (2009)
  • Author(s): 楠見明弘
  • Organizer: 文部科学省科学研究費補助金特定領域研究平成18年度〜22年度非平衡ソフトマター物理学の創成 : メソスコピック系の構造とダイナミクス第3回領域研究
  • Place of Presentation: 福岡
  • Year and Date: 2009-01-09
• [Presentation] Paradigm shift of the plasma membrane dynamics, structure, and signal transduction by single-molecule tracking analysis (2008)
  • Author(s): A. Kusumi
  • Organizer: Kobe University Global COE Program : International Symposium on Integrative Membrane Biology
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-17
• [Presentation] Dynamics of raft-associated molecules for signal transduction as revealed by single-molecule tracking (2008)
  • Author(s): 鈴木健一、楠見明弘
  • Organizer: BMB2008第31回日本分子生物学会年会第81回日本生化学会大会合同年会シンポジウムセッション2S12「糖鎖による生体膜近傍の細胞機能制御機構」
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-10
• [Presentation] ラフト仮説の新展開 : 1分子追跡による研究 (2008)
  • Author(s): 楠見明弘
  • Organizer: BB2008第31回日本分子生物学会年会第81回日本生化学会大会合同大会シンポジウムセッション2S21「膜ドメイン研究の新展開」
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-10
• [Presentation] Three dimensional interplay of the membrane skeleton with the plasma membrane as visualized by freeze-etch electron tomography (2008)
  • Author(s): 諸根信弘、藤原敬宏、笠井倫志、湯浅茂樹、臼倉治郎、楠見明弘
  • Organizer: 第46回日本生物物理学会年会シンポジウム2S2「細胞膜とアクチン骨格との相互作用 : 生物物理のアプローチ」
  • Place of Presentation: 福岡
  • Year and Date: 2008-12-04
• [Presentation] 1分子で見る、細胞膜がはたらく仕組み (2008)
  • Author(s): 楠見明弘
  • Organizer: 19thフォーラム・イン・ドージン「細胞膜脂質のダイナミクス」
  • Place of Presentation: 熊本
  • Year and Date: 2008-11-28
• [Presentation] Single-molecule tracking of transient signal transduction complexes (2008)
  • Author(s): A. Kusumi
  • Organizer: Medical Sciences Congress
  • Place of Presentation: クイーンズランド(ニュージーランド)
  • Year and Date: 2008-11-26
• [Presentation] Single-molecule tracking of transient signal transduction complexes (2008)
  • Author(s): A. Kusumi
  • Organizer: Medical Sciences Congress
  • Place of Presentation: クイーンズランド、ニュージーランド
  • Year and Date: 2008-11-26
• [Presentation] Single-molecule tracking in the living cell plasma membrane: compartments, nanodomains and signaling (2008)
  • Author(s): A. Kusumi
  • Organizer: Principles of Cell and Tissue Organization
  • Place of Presentation: ドレスデン(ドイツ)
  • Year and Date: 2008-11-10
• [Presentation] Single-molecule tracking in the living cell plasma membrane : compartments, nanodomains and signaling (2008)
  • Author(s): A. Kusumi
  • Organizer: Principles of Cell and Tissue Organization
  • Place of Presentation: ドレスデン、ドイツ
  • Year and Date: 2008-11-10
• [Presentation] Compartmentalization of the plasma membrane by the membrane skeleton : high-speed single-molecule tracking study (2008)
  • Author(s): T. Fujiwara and A. Kusumi
  • Organizer: The 9th Asia-Pacific Microscopy Conference
  • Place of Presentation: 済州島、韓国
  • Year and Date: 2008-11-05
• [Presentation] 1分子追跡で見る細胞膜が働くしくみ (2008)
  • Author(s): 楠見明弘
  • Organizer: 日本バイオイメージング学会第17回学術集会
  • Place of Presentation: 千葉
  • Year and Date: 2008-10-31
• [Presentation] Signal transduction based on the plasma membrane meso-domains and the actin membrane skeleton : a single-molecule tracking study (2008)
  • Author(s): A. Kusumi
  • Organizer: New Frontiers in Neurophotonics : 1st Franco-Canadian Symposium
  • Place of Presentation: ボルドー、フランス
  • Year and Date: 2008-10-22
• [Presentation] 細胞膜上のラフトを介したシグナル伝達機構 : 1分子追跡による研究 (2008)
  • Author(s): 鈴木健一、楠見明弘
  • Organizer: 日本顕微鏡学会第52回シンポジウム
  • Place of Presentation: 千葉
  • Year and Date: 2008-10-18
• [Presentation] 1分子観察で初めて見えてくる細胞膜ラフトとシグナル伝達機構 (2008)
  • Author(s): 鈴木健一、楠見明弘
  • Organizer: 第30回北海道大学獣医学学術基金群講演会
  • Place of Presentation: 北海道
  • Year and Date: 2008-10-15
• [Presentation] 1分子追跡によって細胞膜上の足場がはたらく仕組みを解く (2008)
  • Author(s): 楠見明弘
  • Organizer: 文部省科学研究費補助金特定領域研究生体膜トランスポートソームの分子構築と生理機能平成20年度第1回班会議
  • Place of Presentation: 淡路島
  • Year and Date: 2008-09-25
• [Presentation] 1分子追跡で見る細胞膜上のドメインとシグナル変換 (2008)
  • Author(s): 楠見明弘
  • Organizer: 第48回PB夏の学校八王子セミナーハウス
  • Place of Presentation: 東京
  • Year and Date: 2008-07-20
• [Presentation] 1分子追跡法による細胞膜ドメインの動的構造と機能の研究 (2008)
  • Author(s): 楠見明弘
  • Organizer: 第4回次世代バイオイメージング技術開発研究会
  • Place of Presentation: 名古屋
  • Year and Date: 2008-07-18
• [Presentation] ナノデバイスコース・先端計測学 (2008)
  • Author(s): 楠見明弘
  • Organizer: ナノメディシン融合教育ユニット講義
  • Place of Presentation: 京都
  • Year and Date: 2008-07-17
• [Presentation] 1分子追跡と電子線トモグラフィーの相関顕微技術で明らかになった細胞膜のナノ間仕切り構造 (2008)
  • Author(s): 楠見明弘
  • Organizer: 再生医療・臓器再建コースミーティング
  • Place of Presentation: 京都
  • Year and Date: 2008-07-04
• [Presentation] Signal transduction based on the plasma membrane meso-domains and the actin membrane skeleton : a single-molecule tracking study (2008)
  • Author(s): 楠見明弘
  • Organizer: 第60回日本細胞生物学会大会シンポジウム3(S3)「細胞骨格と生体膜のダイナミクス」
  • Place of Presentation: 横浜
  • Year and Date: 2008-06-30
• [Presentation] Single-molecule tracking reveals dynamics of monomer-oligomer equilibrium of GPCRs (2008)
  • Author(s): A. Kusumi
  • Organizer: Meeting : two days with GPCRs in Montpellier
  • Place of Presentation: モンペリエ、フランス
  • Year and Date: 2008-06-13
• [Presentation] 細胞膜システムを見る (2008)
  • Author(s): 楠見明弘
  • Organizer: 奈良県立医科大学アドバンストコース講義
  • Place of Presentation: 奈良
  • Year and Date: 2008-05-30
• [Presentation] Single-molecule tracking in the living cell membrane : signaling in a variety of membrane domains (2008)
  • Author(s): A. Kusumi
  • Organizer: Post IID 2008 Satellite International Meeting on "Autoimmune Bullous Diseases"
  • Place of Presentation: 大津
  • Year and Date: 2008-05-18
• [Presentation] 1分子追跡で見る細胞膜上のシグナル変換 (2008)
  • Author(s): 楠見明弘
  • Organizer: 日本膜学会第30回年会
  • Place of Presentation: 東京
  • Year and Date: 2008-05-15
• [Presentation] Single-molecule imaging of raft-based signal transduction in living cells: a system of digital signal (2008)
  • Author(s): A. Kusumi
  • Organizer: The 38th Seiriken/Sokendai International Conference "Stock and flow of functional molecules in synapse"
  • Place of Presentation: Okazaki, Japan
  • Year and Date: 2008-03-17
• [Presentation] Transient formation of signalling complexes as detected by single-molecule tracking (2007)
  • Author(s): A. Kusumi
  • Organizer: NCBS-ICORP-iCeMS Symposium on Spatial and Temporal Mapping of Membrane Molecules
  • Place of Presentation: Bangalore, India
  • Year and Date: 2007-11-22
• [Presentation] Single-molecule imaging and manipulation in the living cell membrane (2007)
  • Author(s): A. Kusumi
  • Organizer: RIKEN Brain Science Institute Summer Program 2007 "Brain Science: Mystery and Mission"
  • Place of Presentation: Wako, Japan
  • Year and Date: 2007-07-25
• [Presentation] Single-molecule tracking reveals partitionaing of the plasma membrane and signal transduction processes (2007)
  • Author(s): A. Kusumi
  • Organizer: The 3rd Asian and Pacific Rim Symposium on Photonics & Biophotonics
  • Place of Presentation: ケアンズ(オーストラリア)
  • Year and Date: 2007-07-09
• [Presentation] Single-molecule tracking reveals partitionaing of the plasma membrane and signal transduction processes (2007)
  • Author(s): A. Kusumi
  • Organizer: The 3rd Asian and Pacific Rim Symposium on Biophotonics & Biophotonics Downunder II
  • Place of Presentation: Cairns, Australia.
  • Year and Date: 2007-07-09
• [Presentation] Single-molecule tracking in the living cell membrane: compartments and signalling (2007)
  • Author(s): A. Kusumi
  • Organizer: Department Seminar at The Department of Physics, The University of Auckland
  • Place of Presentation: Auckland, New Zealand
  • Year and Date: 2007-07-05
• [Presentation] Electron tomography reveals the plasma membrane compartmentalization as its three dimensional interplay between membrane and cytoskeletal systens (2007)
  • Author(s): N. Morone, T. Fujiwara, K. Murase, R. S. Kasai, H. Ike, S. Yuasa, J. Usukura, and A.Kusumi
  • Organizer: The American Society for Cell Biology 2007 Summer Meetings & European Cytoskelton Forum "Dynamic Interplay Between Cytoskeletal and Membrane Systems"
  • Place of Presentation: Dijion, France
  • Year and Date: 2007-06-28
• [Presentation] Mechanism for raft-based signal transduction as studied by single-molecule tracking (2007)
  • Author(s): K. G. N. Suzuki, and A. Kusumi
  • Organizer: Frontiers in Microscopy II: Imaging From Single Molecules to Whole Organisms and Its Application
  • Place of Presentation: Bar Harbor, U.S.A
  • Year and Date: 2007-06-21
• [Presentation] Single-molecule tracking reveals transient nature of molecular interactions in signal transduction (2006)
  • Author(s): A. Kusumi
  • Organizer: Nature Chemical Biology, The First Annual Symposium "The Chemical Biology of the Cell"
  • Place of Presentation: ボストン(アメリカ)
  • Year and Date: 2006-11-11
• [Presentation] Ligand-induced stabilized raft as a signaling platform for Gpi-anchored receptors: A single-molecule tracking study (2006)
  • Author(s): A. Kusumi
  • Organizer: Keystone Symposia "Lymphocyte Activation and Signaling"
  • Place of Presentation: スチームボートスプリングス(アメリカ)
  • Year and Date: 2006-01-08
• [Presentation] Single-molecule imaging of signal transduction in the plasma membrane of living cells (2005)
  • Author(s): A. Kusumi
  • Organizer: The 2005 Conference of the French Cell Biology Society (SBCF)
  • Place of Presentation: パリ(フランス)
  • Year and Date: 2005-11-07