Interactions of transportsome and cytoskeleton

• Project/Area Number: 17081014
• Research Category: Grant-in-Aid for Scientific Research on Priority Areas
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: Kumamoto University
• Principal Investigator: NAKANISHI Hiroyuki Kumamoto University, 大学院・生命科学研究部, 教授 (80314318)
• Co-Investigator(Kenkyū-buntansha): 上江洲 章吉 熊本大学, 大学院・医学薬学研究部, 助教 (70363520)
• Co-Investigator(Renkei-kenkyūsha): UEZU Akiyoshi 熊本大学, 大学院・生命科学研究部, 助教 (70363520) ; FUJITA Naoyuki 熊本大学, 大学院・生命科学研究部, 准教授 (50403192) ; UMEDA Kazuaki 熊本大学, 大学院・生命科学研究部, 助教 (80444876)
• Project Period (FY): 2005 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥60,500,000 (Direct Cost: ¥60,500,000); Fiscal Year 2009: ¥12,100,000 (Direct Cost: ¥12,100,000); Fiscal Year 2008: ¥12,100,000 (Direct Cost: ¥12,100,000); Fiscal Year 2007: ¥13,700,000 (Direct Cost: ¥13,700,000); Fiscal Year 2006: ¥16,200,000 (Direct Cost: ¥16,200,000); Fiscal Year 2005: ¥6,400,000 (Direct Cost: ¥6,400,000)
• Keywords: 微小管 / 細胞膜 / クラスリン / エンドサイトーシス / 上皮性ナトリウムチャンネル / 中心体 / リポソーム / ユビキチン / ユビキチン化酵素E3 / リン脂質 / インテグリン / ユビキンチン化酵素E3 / 線毛 / ソーティング / 微小管結合蛋白質 / 初期エンドソーム / チュブリン / 微小管結合タンパク質 / 細胞膜変形 / 一次線毛

Research Abstract

We have identified SGIP1・ and its homologous molecules, FCHO1 and FCHO2, as novel microtubule-binding proteins. They bound not only to microtubules but also to membrane phospholipids and deformed membranes to tubules. SGIP1・ and FCHO2 were involved in clathrin-dependent endocytosis. FCHO2 regulated the ubiquitination and endocytosis of epithelial sodium channel (ENaC). In addition, FCHO2, which was also localized in centrosomes, regulated the organization of microtubules.

Research Products

• [Journal Article] Identification and characterization of the novel centrosomal protein centlein. Biochem. (2008)
  • Author(s): Makino, K., Umeda, K., Uezu, A., Hiragami, Y., Sakamoto, T., Ihn, H., Nakanishi, H.
  • Journal Title: Biophys. Res. Commun. 366 Pages: 958-962
  • Peer Reviewed
• [Journal Article] Mass spectrometric analysis of microtubule-cosedimented proteins from rat brain. (2008)
  • Author(s): Sakamoto, T., Uezu, A., Kawauchi, S., Kuramoto, T., Makino, K., Umeda, K., Araki, N., Baba, H., Nakanishi, H.
  • Journal Title: Genes Cells 13 Pages: 295-312
  • Peer Reviewed
• [Journal Article] Frabin and its related Cdc42-specific guanine nucleotide exchange factors couple the actin cytoskeleton with the plasma membrane. (2008)
  • Author(s): Nakanishi, H., Takai, Y.
  • Journal Title: J. Cell. Mol. Med 12 Pages: 1160-1176
  • Peer Reviewed
• [Journal Article] Frabin and its related Cdc42-specific guanine nucleotide exchange factors couple the actin cytoskeleton with the plasma membrane (2008)
  • Author(s): H. Nakanishi and Y. Takai
  • Journal Title: J.Cell.Mol.Med 12 Pages: 1119-1176
  • Peer Reviewed
• [Journal Article] Identification and characterization of the novel centrosomal protein centlein (2008)
  • Author(s): K. Makino, et. al.
  • Journal Title: Biochem. Biophys. Res. Commun. 366 Pages: 958-962
  • Peer Reviewed
• [Journal Article] Mass spectrometric analysis of microtubule-cosedimented proteins from rat brain (2008)
  • Author(s): T. Sakamoto, et. al.
  • Journal Title: Genes to Cells 13 Pages: 295-312
  • Peer Reviewed
• [Journal Article] SGIP1・is an endocytic protein that directly interacts with phospholipids and Eps15. (2007)
  • Author(s): Uezu, A., Horiuchi, A., Kanda, K., Kikuchi, N., Umeda, K., Tsujita, K., Suetsugu, S., Araki, N., Yamamoto, H., Takenawa, H., Nakanishi, H.
  • Journal Title: J. Biol. Chem 282 Pages: 26481-26489
  • Peer Reviewed
• [Journal Article] SGIP1α is an endocytic protein that directly interacts with phospholipids and Eps15 (2007)
  • Author(s): A. Uezu, et. al.
  • Journal Title: J. Biol. Chem. 282 Pages: 26481-26489
  • Peer Reviewed
• [Presentation] 新しいリン脂質結合タンパク質SGIP1αによる初期エンドソームでのEGF受容体のソーティング機構 (2008)
  • Author(s): 上江洲章吉
  • Organizer: BMB2008
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-12
• [Presentation] 新しいリン脂質結合タンパク質SGIP1・による初期エンドソームでのEGF受容体のソーティング機構 (2008)
  • Author(s): 上江洲章吉
  • Organizer: BMB2008
  • Place of Presentation: 神戸ポートアイランド
• [Presentation] SGIP1・is an endocytic protein that directly interacts with phospholipids and Eps15 (2007)
  • Author(s): 上江洲章吉
  • Organizer: BMB2007
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2007-12-12
• [Presentation] SGIP1α is an endocytic protein that directly interacts with phospholipids and Eps15 (2007)
  • Author(s): 上江州章吉
  • Organizer: BMB2007
  • Place of Presentation: 横浜
  • Year and Date: 2007-12-12
• [Presentation] 新しい中心体蛋白質の同定と機能解析 (2007)
  • Author(s): 牧野公治
  • Organizer: BMB2007
  • Place of Presentation: パシフィコ横浜
• [Presentation] 神経突起の形成に関わる新しい微小管結合蛋白質 (2007)
  • Author(s): 菊池直也、Brelin
  • Organizer: BMB2007
  • Place of Presentation: パシフィコ横浜
• [Presentation] 新しい中心体蛋白質の同定と機能解析 (2007)
  • Author(s): 牧野公治
  • Organizer: 日本研究皮膚化学会
  • Place of Presentation: パシフィコ横浜
• [Presentation] SGIP1・による膜輸送の制御 (2006)
  • Author(s): 堀内綾香
  • Organizer: 第59回日本薬理学学会西南部会
  • Place of Presentation: 沖縄(メルパルク沖縄)
  • Year and Date: 2006-11-24
• [Presentation] 新しい微小管結合タンパク質による中心体の機能制御 (2006)
  • Author(s): 坂本達彦
  • Organizer: 第65回日本癌学会学術総会
  • Place of Presentation: パシフィコ横浜
• [Remarks]
  • URL: http://srv02.medic.kumamoto-u.ac.jp/dept/pharma1/pharma1.html