Visualization and analysis of membrane fusion mechanism during vesicular transport of transportosome by using multi-photon excitation process
| Project/Area Number |
17081017
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| Research Category |
Grant-in-Aid for Scientific Research on Priority Areas
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| Allocation Type | Single-year Grants |
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| Review Section |
Biological Sciences
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| Research Institution | National Institute for Physiological Sciences |
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Principal Investigator |
NEMOTO Tomomi National Institute for Physiological Sciences, 電子科学研究所, 教授 (50291084)
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| Co-Investigator(Renkei-kenkyūsha) |
KANEMATSU Takashi 広島大学, 大学院・医歯薬学総合研究科, 教授 (10264053)
TAKAHASHI Nobuyuki 京都大学, 大学院・農学系研究科, 助教 (50370135)
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| Project Period (FY) |
2005 – 2009
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| Project Status |
Completed (Fiscal Year 2009)
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| Budget Amount *help |
¥59,900,000 (Direct Cost: ¥59,900,000)
Fiscal Year 2009: ¥7,700,000 (Direct Cost: ¥7,700,000)
Fiscal Year 2008: ¥12,200,000 (Direct Cost: ¥12,200,000)
Fiscal Year 2007: ¥13,100,000 (Direct Cost: ¥13,100,000)
Fiscal Year 2006: ¥20,200,000 (Direct Cost: ¥20,200,000)
Fiscal Year 2005: ¥6,700,000 (Direct Cost: ¥6,700,000)
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| Keywords | 生理学 / 高機能レーザー / 生体分子 / 発生・分化 / 生物物理 / 開口放出 / 急性膵炎 / インシュリン / エンドサイトーシス / グルコース輸送体 / 蛍光タンパク質 / 高性能レーザー / 2光子顕微鏡 / 生物物理学 / 細胞生理学 / カルシウムシグナル / 外分泌腺 |
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| Research Abstract |
Physiological functions of membrane transporter are supposed to be closely related to vesicular transport. However, membrane fusion mechanism underlying vital functions including exocytosis remains unclear. In order to clarify the molecular basis, we have developed a new light microscopy, two-photon microscopy, which is realized by using non-linear optics, multi-photon excitation process. By using two-photon microscopy, we have established new methods, which enable us to visualize finer structures inside a living organ, and we have obtained new important insights on molecular mechanism of exocytosis, and on physiological and pathological roles of membrane fusion.
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Report
(6 results)
Research Products
(103 results)
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[Journal Article] Rap1 controls lymphocyte adhesion cascade and interstitial migration within lymph nodes in a RAPL-dependent and -independent manner.2010
Author(s)
Yukihiko Ebisuno, Koko Katagiri, Tomoya Katakai, Yoshihiro Ueda, Tomomi Nemoto, Hiroyuki Inada, Junichi Nabekura, Takaharu Okada, Reiji Kannagi, Toshiyuki Tanaka, Masayuki Miyasaka, Nancy Hogg, Tatsuo Kinashi
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Journal Title
Blood 115(4)
Pages: 804-14
Related Report
Peer Reviewed
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[Journal Article] Pancreas-specific aquaporin 12 null mice showed increased susceptibility to caerulein-induced acute pancreatitis2009
Author(s)
Eriko Ohta, Tomohiro Itoh, Tomomi Nemoto, Jiro Kumagai, Shigeru B.H. Ko, Kenichi Ishibashi, Mayuko Ohno, Keiko Uchida, Akihito Ohta, Eisei Sohara, Shinichi Uchida, Sei Sasaki, Tatemitsu Rai
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Journal Title
Am. J. Physiol. Cell Physiol. 297(6)
Related Report
Peer Reviewed
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