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Visualization and analysis of membrane fusion mechanism during vesicular transport of transportosome by using multi-photon excitation process

Research Project

Project/Area Number 17081017
Research Category

Grant-in-Aid for Scientific Research on Priority Areas

Allocation TypeSingle-year Grants
Review Section Biological Sciences
Research InstitutionNational Institute for Physiological Sciences

Principal Investigator

NEMOTO Tomomi  National Institute for Physiological Sciences, 電子科学研究所, 教授 (50291084)

Co-Investigator(Renkei-kenkyūsha) KANEMATSU Takashi  広島大学, 大学院・医歯薬学総合研究科, 教授 (10264053)
TAKAHASHI Nobuyuki  京都大学, 大学院・農学系研究科, 助教 (50370135)
Project Period (FY) 2005 – 2009
Project Status Completed (Fiscal Year 2009)
Budget Amount *help
¥59,900,000 (Direct Cost: ¥59,900,000)
Fiscal Year 2009: ¥7,700,000 (Direct Cost: ¥7,700,000)
Fiscal Year 2008: ¥12,200,000 (Direct Cost: ¥12,200,000)
Fiscal Year 2007: ¥13,100,000 (Direct Cost: ¥13,100,000)
Fiscal Year 2006: ¥20,200,000 (Direct Cost: ¥20,200,000)
Fiscal Year 2005: ¥6,700,000 (Direct Cost: ¥6,700,000)
Keywords生理学 / 高機能レーザー / 生体分子 / 発生・分化 / 生物物理 / 開口放出 / 急性膵炎 / インシュリン / エンドサイトーシス / グルコース輸送体 / 蛍光タンパク質 / 高性能レーザー / 2光子顕微鏡 / 生物物理学 / 細胞生理学 / カルシウムシグナル / 外分泌腺
Research Abstract

Physiological functions of membrane transporter are supposed to be closely related to vesicular transport. However, membrane fusion mechanism underlying vital functions including exocytosis remains unclear. In order to clarify the molecular basis, we have developed a new light microscopy, two-photon microscopy, which is realized by using non-linear optics, multi-photon excitation process. By using two-photon microscopy, we have established new methods, which enable us to visualize finer structures inside a living organ, and we have obtained new important insights on molecular mechanism of exocytosis, and on physiological and pathological roles of membrane fusion.

Report

(6 results)
  • 2009 Annual Research Report   Final Research Report ( PDF )
  • 2008 Annual Research Report
  • 2007 Annual Research Report
  • 2006 Annual Research Report
  • 2005 Annual Research Report
  • Research Products

    (103 results)

All 2010 2009 2008 2007 2006 2005 Other

All Journal Article (34 results) (of which Peer Reviewed: 20 results) Presentation (60 results) Book (5 results) Remarks (2 results) Patent(Industrial Property Rights) (2 results)

  • [Journal Article] Rap1 controls lymphocyte adhesion cascade and interstitial migration within lymph nodes in a RAPL-dependent and -independent manner.2010

    • Author(s)
      Yukihiko Ebisuno, Koko Katagiri, Tomoya Katakai, Yoshihiro Ueda, Tomomi Nemoto, Hiroyuki Inada, Junichi Nabekura, Takaharu Okada, Reiji Kannagi, Toshiyuki Tanaka, Masayuki Miyasaka, Nancy Hogg, Tatsuo Kinashi
    • Journal Title

      Blood 115(4)

      Pages: 804-14

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] レーザーを用いた開口放出や細胞膜機能・形態の新規的な可視化手法2010

    • Author(s)
      根本知己
    • Journal Title

      Vol.35

      Pages: 57-62

    • Related Report
      2009 Final Research Report
  • [Journal Article] Rap1 controls lymphocyte adhesion cascade and interstitial migration within lymph nodes in a RAPL-dependent and -ind ependent manner2010

    • Author(s)
      Yukihiko Ebisuno
    • Journal Title

      Blood 115(4)

      Pages: 804-14

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Potential of "in vivo" Two-photon microscopy in visualization of 3D structure and functional information in living body2010

    • Author(s)
      根本知己
    • Journal Title

      J.Pharmacolog.Sci. 112

      Pages: 45-45

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] レーザーを用いた開口放出や細胞膜機能・形態の新規的な可視化手法2010

    • Author(s)
      根本知己
    • Journal Title

      膜 35

      Pages: 57-62

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 超短光パルスレーザーによる細胞、生体分子の動態の可視化解析2010

    • Author(s)
      根本知己
    • Journal Title

      レーザー医学会誌 30

      Pages: 435-440

    • NAID

      10026089167

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Pancreas-specific aquaporin 12 null mice showed increased susceptibility to caerulein-induced acute pancreatitis2009

    • Author(s)
      Eriko Ohta, Tomohiro Itoh, Tomomi Nemoto, Jiro Kumagai, Shigeru B.H. Ko, Kenichi Ishibashi, Mayuko Ohno, Keiko Uchida, Akihito Ohta, Eisei Sohara, Shinichi Uchida, Sei Sasaki, Tatemitsu Rai
    • Journal Title

      Am. J. Physiol. Cell Physiol. 297(6)

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] An ultramarine fluorescent protein with high photostability and pH insensitivity2009

    • Author(s)
      Wataru Tomosugi, Tomoki Matsuda, Tomomi Tani, Ippei Kotera, Kenta Saito, Kazuki Horikagwa, Tomomi Nemoto, Takeharu Nagai
    • Journal Title

      Nature Methods vol.6

      Pages: 351-353

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Differential Activity-Dependent Secretion of Brain-Derived Neurotrophic Factor from Axon and Dendrite2009

    • Author(s)
      Naoto Matsuda
    • Journal Title

      J.Neurosci. 29(45)

      Pages: 14185-98

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Pancreas-specific aquaporin 12 null mice showed increased susceptibility to caerulein-induced acute pancreatitis2009

    • Author(s)
      Eriko Ohta
    • Journal Title

      Am.J.Physiol.Cell Physiol. 297(6)

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Maternal separation decreases the stability of mushroom spines in adult mice somatosensory cortex2009

    • Author(s)
      Yusuke Takatsuru
    • Journal Title

      Brain Res. 1294

      Pages: 45-51

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Neuronal circuit remodeling in the contralateral cortical hemisphere during functional recovery from cerebral infarction2009

    • Author(s)
      Yusuke Takatsuru
    • Journal Title

      J.Neurosci. 29

      Pages: 10081-6

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] An ultramarine fluorescent protein with high photostability and pH insensitivity2009

    • Author(s)
      Wataru Tomosugi
    • Journal Title

      Nature Methods 6

      Pages: 351-353

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 光の回折限界を超える蛍光イメージング技術2009

    • Author(s)
      根本知己
    • Journal Title

      ぶんせき 409

      Pages: 8-13

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Living cell functions and morphology revealed by two-photon microscopy in intact neural and secretory organs2008

    • Author(s)
      Tomomi Nemoto
    • Journal Title

      Mol. Cells vol.26

      Pages: 113-120

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Living cell functions and morphology revealed by two-photon microscopy in intact neural and secretory organ2008

    • Author(s)
      Tomomi Nemoto
    • Journal Title

      Mol. Cell 26

      Pages: 113-120

    • Related Report
      2008 Annual Research Report
  • [Journal Article] 3光子励起過程と2光子励起過程を同時に用いた外分泌腺の自家蛍光イメージング2008

    • Author(s)
      根本知己
    • Journal Title

      細胞工学 27

      Pages: 861-861

    • Related Report
      2008 Annual Research Report
  • [Journal Article] 2光子顕微鏡による膜動態可視化解析技術の新展開2008

    • Author(s)
      根本知己
    • Journal Title

      薬学雑誌 128

      Pages: 513-520

    • NAID

      110006656190

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Two cAMP-dependent pathways differentially regulate exocytosis of large dense-core and small vesicles in beta cells2007

    • Author(s)
      Hiroyasu Hatakeyama, Noriko Takahashi, Takuya Kishimoto, Tomomi Nemoto, Haruo Kasai
    • Journal Title

      J. Physiol. vol.582

      Pages: 1087-98

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Two cAMP-dependent pathways differentially regulate exocytosis of large dens-core and small vesicles in beta cells2007

    • Author(s)
      Hiroyasu Hatakeyama,
    • Journal Title

      J. Physiol 582

      Pages: 1087-98

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] New advances in Nanomedicine: Diagnosis and Preventive Medicine2007

    • Author(s)
      Chiming Wei
    • Journal Title

      Med. Clin. N. Am. 91

      Pages: 871-879

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 2光子顕微鏡による耐in vivo可視化技術2007

    • Author(s)
      根本知己
    • Journal Title

      別冊化学「分子イメージング最前線 : 蛍光プローブが拓くライフサイエンスの未来」

      Pages: 77-82

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Vacuolar sequential exocytosis of large dense-core vesicles in adrenal medulla2006

    • Author(s)
      Takuya Kishimoto, Ryoichi Kimura, Ting-Ting Liu, Tomomi Nemoto, Noriko Takahashi, Haruo Kasai
    • Journal Title

      EMBO J. vol.25

      Pages: 673-82

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Rapid glucose sensing by protein kinase A for insulin exocytosis in mouse pancreatic islets2006

    • Author(s)
      Hatakeyama H
    • Journal Title

      J Physiol 570

      Pages: 271-282

    • Related Report
      2006 Annual Research Report 2005 Annual Research Report
  • [Journal Article] Vacuolar sequential exocytosis of large dense-core vesicles in adrenal medulla2006

    • Author(s)
      Kishimoto T
    • Journal Title

      EMBO J 25

      Pages: 673-682

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Two-photon excitation imaging of exocytosis and endocytosis and their spatial organizations2006

    • Author(s)
      Kasai H
    • Journal Title

      Adv. Drug Deliv. Rev 58

      Pages: 850-877

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Two-photon microscopic analysis of acetylcholine-induced mucus secretion in guinea pig nasal glands2005

    • Author(s)
      Akihiro Oshima, Tatsuya Kojima, Kenji Dejima, Yasuo Hisa, Haruo Kasai, Tomomi Nemoto
    • Journal Title

      Cell Calcium vol.37

      Pages: 349-357

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Two-photon microscopic analysis of acetylcholine-induced mucus secretion in guinea pig nasal glands2005

    • Author(s)
      Oshima, A.
    • Journal Title

      Cell Calcium 37

      Pages: 349-357

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Rapid Ca2+-dependent increase in oxygen consumption by mitochondria in single mammalian central neurons2005

    • Author(s)
      Hayakawa, Y.
    • Journal Title

      Cell Calcium 37

      Pages: 359-370

    • Related Report
      2005 Annual Research Report
  • [Journal Article] A new quantitative (TEPIQ) analysis for diameters of exocytic vesicles and its application to pancreatic islets2005

    • Author(s)
      Kasai H
    • Journal Title

      J Physiol 568

      Pages: 891-903

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Sequential compound exocytosis of large dense-core vesicles in PC12 cells studied with TEPIQ (two-photon extracellular polar-tracer imaging-based quantification) analysis2005

    • Author(s)
      Kishimoto T
    • Journal Title

      J Physiol 568

      Pages: 905-915

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Exocytosis and endocytosis of small vesicles in PC12 cells studied with TEPIQ (two-photon extracellular polar-tracer imaging-based quantification) analysis.2005

    • Author(s)
      Liu TT
    • Journal Title

      J Physiol 568

      Pages: 917-929

    • Related Report
      2005 Annual Research Report
  • [Journal Article] 分泌腺細胞の開口放出の分子機構-多光子励起過程を用いた可視化解析によりみえてきたもの.2005

    • Author(s)
      根本知己
    • Journal Title

      蛋白質核酸酵素 50(8)

      Pages: 949-957

    • NAID

      40006813109

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Vacuolar sequential exocytosis of large dense-core vesicles in adrenal medulla

    • Author(s)
      Kishimoto T
    • Journal Title

      EMBO J (In press)

    • Related Report
      2005 Annual Research Report
  • [Presentation] two-photon Microscopic Analysis of Secretion and Fine Intracellular Structure of Living Cell2010

    • Author(s)
      根本知己
    • Organizer
      International Symposium of Joint Research Network on Advanced Materials and Devices
    • Place of Presentation
      ホテルニドム、苫小牧市
    • Year and Date
      2010-03-25
    • Related Report
      2009 Annual Research Report
  • [Presentation] Recent develdpment in functional analysis of biological specimen by using two-photon microscopy2010

    • Author(s)
      Terumasa Hibi
    • Organizer
      International Symposium of Joint Research Network on Advanced Materials and Devices
    • Place of Presentation
      ホテルニドム、苫小牧市
    • Year and Date
      2010-03-25
    • Related Report
      2009 Annual Research Report
  • [Presentation] 輸送小胞、膜融合の生理機能における多光子イメージングの展開2010

    • Author(s)
      根本知己
    • Organizer
      生体膜輸送体特定班公開シンポジウム
    • Place of Presentation
      大阪市
    • Year and Date
      2010-03-19
    • Related Report
      2009 Annual Research Report
  • [Presentation] Enhancement of lateral resolution of confocal and two-photon laser scanning microscopy by using higher-order radially polarized laser beams2010

    • Author(s)
      T. Hibi, Y Kozawa, A. Sato, H. Yokoyama, S. Sato, T. Nemoto
    • Organizer
      Focus on Microscopy 2010
    • Place of Presentation
      Shanghai, China
    • Related Report
      2009 Final Research Report
  • [Presentation] 多光子顕微鏡を用いた生きた個体内部での細胞機能・活性のin vivo可視化解析2010

    • Author(s)
      根本知己
    • Organizer
      第83回日本薬理学会年会
    • Place of Presentation
      大阪国際会議場
    • Related Report
      2009 Final Research Report
  • [Presentation] 多光子顕微鏡を用いた生きた個体内部での細胞機能・活性の in vivo 可視化解析2010

    • Author(s)
      根本知己
    • Organizer
      第83回日本薬理学会年会
    • Place of Presentation
      大阪国際会議場
    • Related Report
      2009 Annual Research Report
  • [Presentation] 多光子励起法による初代培養肝細胞内CYP酵素活性の測定2010

    • Author(s)
      岸本拓哉
    • Organizer
      日本薬学会第130回年会
    • Place of Presentation
      岡山市
    • Related Report
      2009 Annual Research Report
  • [Presentation] インビボイメージングにおける2光子顕微鏡。非線形光学顕微鏡の特徴と可能性2009

    • Author(s)
      根本知己
    • Organizer
      第32回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜、横浜市
    • Year and Date
      2009-12-12
    • Related Report
      2009 Annual Research Report 2009 Final Research Report
  • [Presentation] 2光子顕微鏡法の原理と応用2009

    • Author(s)
      根本知己
    • Organizer
      第14回細胞生物学ワークショップ
    • Place of Presentation
      北海道大学、札幌市
    • Year and Date
      2009-11-24
    • Related Report
      2009 Annual Research Report
  • [Presentation] 2光子顕微鏡による生体計測2009

    • Author(s)
      根本知己
    • Organizer
      第7回医用分光学研究会、東邦大学医療センター大橋病院
    • Place of Presentation
      東京都
    • Year and Date
      2009-11-07
    • Related Report
      2009 Final Research Report
  • [Presentation] 2光子顕微鏡による生体計測2009

    • Author(s)
      根本知己
    • Organizer
      第7回医用分光学研究会
    • Place of Presentation
      東邦大学医療センター大橋病院、東京都
    • Year and Date
      2009-11-07
    • Related Report
      2009 Annual Research Report
  • [Presentation] 多光子顕微鏡による深部組織のin vivo(生きたまま)イメージングの技術2009

    • Author(s)
      根本知己
    • Organizer
      レーザー学会東京支部秋セミナー
    • Place of Presentation
      慶応義塾大学理工学部、横浜市
    • Year and Date
      2009-10-30
    • Related Report
      2009 Final Research Report
  • [Presentation] 多光子顕微鏡による深部組織の in vivo(生きたまま)イメージングの技術2009

    • Author(s)
      根本知己
    • Organizer
      レーザー学会東京支部秋セミナー
    • Place of Presentation
      慶応義塾大学理工学部、横浜市
    • Year and Date
      2009-10-03
    • Related Report
      2009 Annual Research Report
  • [Presentation] 超短光パルスレーザーを用いた細胞生理機能の可視化解析技術の展開2009

    • Author(s)
      根本知己
    • Organizer
      若手研究者支援のための産学協同GCOEシンポジウム2009
    • Place of Presentation
      札幌京王ホテル、札幌市
    • Year and Date
      2009-10-01
    • Related Report
      2009 Annual Research Report
  • [Presentation] 非侵襲的なバイオ分子機能イメージングを可能とするパルスレーザーを用いた光学顕微鏡法の開発2009

    • Author(s)
      根本知己
    • Organizer
      第1回 BioOpto Japan カンファレンス
    • Place of Presentation
      パシフィコ横浜・横浜市
    • Year and Date
      2009-09-17
    • Related Report
      2009 Annual Research Report
  • [Presentation] 計測・解析法1(細胞イメージング)2009

    • Author(s)
      根本知己
    • Organizer
      日本生理学会主催生理学若手サマースクール2009
    • Place of Presentation
      岡崎コンファレンスセンター、岡崎市
    • Year and Date
      2009-09-14
    • Related Report
      2009 Annual Research Report
  • [Presentation] 光の回折限界を超える細胞機能イメージングの試み2009

    • Author(s)
      根本知己
    • Organizer
      生物物理若手の会第49回夏の学校
    • Place of Presentation
      支笏湖ユースホステル、千歳市
    • Year and Date
      2009-08-01
    • Related Report
      2009 Annual Research Report
  • [Presentation] 多光子励起顕微雛による細胞機能の分子イメージング2009

    • Author(s)
      根本知己
    • Organizer
      金沢大学十全医学会総会・学術集会
    • Place of Presentation
      金沢大学医学部記念館、金沢市
    • Year and Date
      2009-07-25
    • Related Report
      2009 Annual Research Report
  • [Presentation] カルシウム依存性開口放出の可視化解析2009

    • Author(s)
      根本知己
    • Organizer
      日本薬学会東海支部特別講演会
    • Place of Presentation
      名古屋市立大学薬学部、名古屋市
    • Year and Date
      2009-05-21
    • Related Report
      2009 Annual Research Report
  • [Presentation] ニューロンとグリアの生体イメージング2009

    • Author(s)
      鍋倉淳一
    • Organizer
      第4回イメージングサイエンスシンポジウム
    • Place of Presentation
      岡崎、日本
    • Year and Date
      2009-03-24
    • Related Report
      2008 Annual Research Report
  • [Presentation] 非線形光学を利用したバイオ分子イメージング〜2光子顕微鏡によるin vivoイメージングの展開2009

    • Author(s)
      根本知己
    • Organizer
      ソニーオープンラボ第3回講演会
    • Place of Presentation
      東京、日本
    • Year and Date
      2009-03-04
    • Related Report
      2008 Annual Research Report
  • [Presentation] 2光子顕微鏡を用いた細胞機能計測とin vivoイメージング2009

    • Author(s)
      根本知己
    • Organizer
      防衛医科大学「バイオを考える」シンポジウム
    • Place of Presentation
      所沢市、日本
    • Year and Date
      2009-02-27
    • Related Report
      2008 Annual Research Report
  • [Presentation] 多光子励起過程を用いた細胞機能・動態のin vivo可視化2009

    • Author(s)
      根本知己
    • Organizer
      環境医学研究所合同シンポジウム
    • Place of Presentation
      名古屋、日本
    • Year and Date
      2009-02-16
    • Related Report
      2008 Annual Research Report
  • [Presentation] Recent development in microscopic analysis using two-photon excitation and non-linear process2009

    • Author(s)
      Tomomi Nemoto
    • Organizer
      ISNM2009 and Asian Core Symposium-Nano and Biomedical Molecular Science
    • Place of Presentation
      岡崎、日本
    • Year and Date
      2009-02-06
    • Related Report
      2008 Annual Research Report
  • [Presentation] Neural and secretory activities revealed by two-photon microscopy2009

    • Author(s)
      Tomomi Nemoto
    • Organizer
      The 6th Asian Biophysical Association (ABA) Symposium, Hong Kong University of Science and Technology
    • Place of Presentation
      Hong-Kong, China
    • Related Report
      2009 Final Research Report
  • [Presentation] PANCREAS-SPECIFIC AQUAPORIN 12 NULL MICE SHOWED INCREASED SUSCEPTIBILITY TO CAERULEIN-INDUCED RCUTE PANCREATITIS2009

    • Author(s)
      Eriko Ohta
    • Organizer
      The 36th Congress of the International Union of Physiological Sciences
    • Place of Presentation
      Kyoto International Conference Center, Kyoto, Japan
    • Related Report
      2009 Annual Research Report
  • [Presentation] PANCREAS-SPECIFIC AQUAPORIN 12 NULL MICE SHOWED INCREASED SUSCEPTIBILITY TO CAERULEIN-INDUCED ACUTE PANCREATITIS2009

    • Author(s)
      Eriko Ohta
    • Organizer
      World Congress of Nephrology 2009
    • Place of Presentation
      Milan, Italy
    • Related Report
      2009 Annual Research Report
  • [Presentation] Sensory input dependent remodeling of cell composition in the adult olfactory bulb glomeruli2009

    • Author(s)
      Masato Sawada
    • Organizer
      第32回日本神経科学大会
    • Place of Presentation
      名古屋国際会議場、名古屋市
    • Related Report
      2009 Annual Research Report
  • [Presentation] PANCREAS-SPECIFIC AQUAPORIN 12 NULL MICE SHOWED INCREASED SUSCEPTIBILITY TO CAERULEIN-INDUCED ACUTE PANCREATITIS2009

    • Author(s)
      Eriko Ohta
    • Organizer
      第52回日本腎臓学会学術総会
    • Place of Presentation
      パシフィコ横浜、横浜市
    • Related Report
      2009 Annual Research Report
  • [Presentation] Neural and secretory activities revealed by two-photon microscopy2009

    • Author(s)
      Tomomi Nemoto
    • Organizer
      The 6th Asian Biophysical Association (ABA) Symposium
    • Place of Presentation
      Hong-Kong, China
    • Related Report
      2008 Annual Research Report
  • [Presentation] Deficiency of PRIP gene in mice exhibits a phenotype of hyperinsulinemia2009

    • Author(s)
      兼松隆
    • Organizer
      第82回薬理学会年会
    • Place of Presentation
      横浜、日本
    • Related Report
      2008 Annual Research Report
  • [Presentation] Two-photon microscopy for in-vivo molecular cell biology of neural and secretory function2008

    • Author(s)
      根本知己
    • Organizer
      国立遺伝学研究所, NIG Biological Symposium
    • Place of Presentation
      三島、日本
    • Year and Date
      2008-12-24
    • Related Report
      2008 Annual Research Report
  • [Presentation] 非線形光学と細胞機能計測2008

    • Author(s)
      根本知己
    • Organizer
      多次元共同脳科学推進センターシンポジウム
    • Place of Presentation
      東京、日本
    • Year and Date
      2008-12-06
    • Related Report
      2008 Annual Research Report
  • [Presentation] Development in imaging and stimulation by using multi-photon excitation process2008

    • Author(s)
      Tomomi Nemoto
    • Organizer
      2008 Annual Meeting of the Spectroscopical Society of Japan
    • Place of Presentation
      仙台、日本
    • Year and Date
      2008-11-21
    • Related Report
      2008 Annual Research Report
  • [Presentation] The dendritic spine of layer V pyramidal neuron in contralateral area of focal ischemia was actively remodeling in somatosensory cortex2008

    • Author(s)
      Yusuke Takatsuru
    • Organizer
      Neuroscience 2008
    • Place of Presentation
      Washington DC, United States
    • Year and Date
      2008-11-16
    • Related Report
      2008 Annual Research Report
  • [Presentation] 2光子顕微鏡による神経グリア細胞動態のニューロフォトニクス2008

    • Author(s)
      根本知己
    • Organizer
      第29回日本レーザー医学会総会
    • Place of Presentation
      東京、日本
    • Year and Date
      2008-11-15
    • Related Report
      2008 Annual Research Report
  • [Presentation] Potential of two-photon microscopy for analysis of living organ2008

    • Author(s)
      Tomomi Nemoto
    • Organizer
      39^<th> NIPS International Symposium & 7^<th> OIB Symposium
    • Place of Presentation
      岡崎、日本
    • Year and Date
      2008-11-10
    • Related Report
      2008 Annual Research Report
  • [Presentation] In vivo functional imaging for secretion and neural activities by two-photon microscopy2008

    • Author(s)
      Tomomi Nemoto
    • Organizer
      The 9th International Congress of Cell Biology and KSMBM
    • Place of Presentation
      Seoul, Korea
    • Year and Date
      2008-10-11
    • Related Report
      2009 Final Research Report
  • [Presentation] in vivo functional imaging for secretion and neural activities by two-photon microscopy2008

    • Author(s)
      Tomomi Nemoto
    • Organizer
      The 9^<th> International Congress of Cell Biology
    • Place of Presentation
      Seoul, Korea
    • Year and Date
      2008-10-11
    • Related Report
      2008 Annual Research Report
  • [Presentation] 多光子励起過程を用いた非侵襲的な可視化技術の展開2008

    • Author(s)
      根本知己
    • Organizer
      第23回生体・生理工学シンポジウム
    • Place of Presentation
      名古屋、日本
    • Year and Date
      2008-09-29
    • Related Report
      2008 Annual Research Report
  • [Presentation] 2光子顕微鏡によるバイオイメージングの展開と細胞機能の解析への応用」、2008

    • Author(s)
      根本 知己
    • Organizer
      第8回情報バイオトロニクス講演会、
    • Place of Presentation
      仙台市、東北大学
    • Year and Date
      2008-01-11
    • Related Report
      2007 Annual Research Report
  • [Presentation] 2光子顕微鏡による神経・分泌機能の可視化技術の展開2008

    • Author(s)
      根本知己
    • Organizer
      第85回生理学会大会
    • Place of Presentation
      京王プラザホテル、東京
    • Related Report
      2009 Final Research Report
  • [Presentation] 生体中の細胞・分子機能ライブイメージング2008

    • Author(s)
      根本知己
    • Organizer
      原子・分子・光科学(AMO)討論会
    • Place of Presentation
      東京、日本
    • Related Report
      2008 Annual Research Report
  • [Presentation] Principle and potential of two-photon microscopy2008

    • Author(s)
      根本知己
    • Organizer
      The 31^<st> Annual Meeting of The Japan Society of Neuroscience
    • Place of Presentation
      東京、日本
    • Related Report
      2008 Annual Research Report
  • [Presentation] 多光子顕微鏡による蛍光イメージングの技術的展開2008

    • Author(s)
      根本知己
    • Organizer
      平成20年度特定領域「生体膜輸送体」第1回班会議
    • Place of Presentation
      淡路市, 日本
    • Related Report
      2008 Annual Research Report
  • [Presentation] 多光子顕微鏡による細胞内膜小胞系動態の可視化2008

    • Author(s)
      根本知己
    • Organizer
      H. 20年度特定領域「生体膜輸送体」第1回班会議
    • Place of Presentation
      淡路市, 日本
    • Related Report
      2008 Annual Research Report
  • [Presentation] 2光子顕微鏡による神経・分泌機能の可視化技術の展開2008

    • Author(s)
      根本 知己
    • Organizer
      第85回生理学会大会
    • Place of Presentation
      東京 京王プラザホテル
    • Related Report
      2007 Annual Research Report
  • [Presentation] 2光子顕微鏡による生細胞機能の蛍光イメージング2007

    • Author(s)
      根本 知己
    • Organizer
      日本分光学会「生細胞分光部会」シンポジウム
    • Place of Presentation
      東京工業大学、横浜市
    • Year and Date
      2007-12-14
    • Related Report
      2007 Annual Research Report
  • [Presentation] 超短光パルスレーザーによる多光子励起過程を用いた細胞機能のイメージング2007

    • Author(s)
      根本 知己
    • Organizer
      東京工業大学大学院理学系研究科物性物理学専攻松下研究室セミナー
    • Place of Presentation
      東京工業大学、東京都
    • Year and Date
      2007-07-20
    • Related Report
      2007 Annual Research Report
  • [Presentation] レーザー顕微鏡の現状と可能性-2光子顕微鏡を中心に2007

    • Author(s)
      根本 知己
    • Organizer
      第1回「生体分子イメージングの新たな技術開発に関する研究会
    • Place of Presentation
      愛知県産業貿易館、名古屋市
    • Year and Date
      2007-06-28
    • Related Report
      2007 Annual Research Report
  • [Presentation] in vivo functional imaging of neural and secretory activities by using two-photon microscopy2007

    • Author(s)
      根本 知己
    • Organizer
      第40回日本発生生物学会・第59回細胞生物学会合同大会
    • Place of Presentation
      福岡国際会議場、福岡市
    • Year and Date
      2007-05-29
    • Related Report
      2007 Annual Research Report
  • [Presentation] 多光子励起法による大脳皮質のin vivo imaging2007

    • Author(s)
      和氣弘明
    • Organizer
      平成19年度生理学研究会「グリア細胞による脳機能調節機構の解明 新しいグリア研究の手がかりを求めて
    • Place of Presentation
      岡崎市
    • Year and Date
      2007-04-26
    • Related Report
      2007 Annual Research Report
  • [Presentation] 2光子顕微鏡によるin vivo imaging2007

    • Author(s)
      和氣弘明
    • Organizer
      第5回理研・分子研合同シンポジウム エクストリームフォトニクス研究-バイオイメージング-
    • Place of Presentation
      理化学研究所,和光市
    • Year and Date
      2007-04-17
    • Related Report
      2007 Annual Research Report
  • [Presentation] Two-photon microscopy for in vivo analysis of neural and secretory activities2007

    • Author(s)
      Tomomi Nemoto
    • Organizer
      Third Annual Conference of the American Academy of Nanomedicine (AANM)
    • Place of Presentation
      San Diego, United States
    • Related Report
      2009 Final Research Report
  • [Presentation] in vivo functional imaging of neural and secretory activities by two-photon microscopy2007

    • Author(s)
      Tomomi Nemoto
    • Organizer
      China-Japan Symposium of Nano-Chemical Biology
    • Place of Presentation
      北京、中華人民共和国
    • Related Report
      2007 Annual Research Report
  • [Presentation] Two-photon microscopy for in vivo analysis of neural and secretory activities2007

    • Author(s)
      Tomomi Nemoto
    • Organizer
      Third Annual Conference of the American Academy of Nanomedicine
    • Place of Presentation
      San Diego, U. S. A.
    • Related Report
      2007 Annual Research Report
  • [Presentation] Two-photon microscopy for in vivo deep imaging of the structure and function of living cells and tissues2007

    • Author(s)
      Tomomi Nemoto
    • Organizer
      1st International Symposium on Nanomedicine -from Basic to Applications-(ISNM2007) & 2nd Molecule-Based Information Transmission and Reception(MB-ITR2007)
    • Place of Presentation
      岡崎、日本
    • Related Report
      2007 Annual Research Report
  • [Presentation] 2光子顕微鏡を用いた神経・分泌細胞の機能解析2007

    • Author(s)
      根本 知己
    • Organizer
      第45回生物物理学会年会
    • Place of Presentation
      パシフィコ横浜、横浜市
    • Related Report
      2007 Annual Research Report
  • [Presentation] 2光子顕微鏡によるin vivoイメージング技術の将来2007

    • Author(s)
      根本 知己
    • Organizer
      日本光学会年次学術講演会
    • Place of Presentation
      大阪大学、吹田市
    • Related Report
      2007 Annual Research Report
  • [Presentation] Two-photon functional imaging of neurons and secretory gland cells2006

    • Author(s)
      Tomomi Nemoto
    • Organizer
      EABS & BSJ 2006
    • Place of Presentation
      Okinawa, Japan
    • Related Report
      2009 Final Research Report
  • [Book] Nanomedicine Science and Engineering (ed. M.J. Schulz), Ch. 20:Two Photon Microscopy for in vivo Analysis of Neural and Secretory Activities2010

    • Author(s)
      Tomomi Nemoto
    • Publisher
      Artech House, Inc.(印刷中)
    • Related Report
      2009 Final Research Report
  • [Book] ナノメディシン-ナノテクの医療応用(宇理須恒雄(編)):2光子顕微鏡を用いた生体組織細胞機能の非侵襲的な可視化解析法2008

    • Author(s)
      根本知己
    • Publisher
      (株)オーム社
    • Related Report
      2009 Final Research Report
  • [Book] 2光子顕微鏡を用いた生体組織細胞機能の非侵襲的な可視化解析法2008

    • Author(s)
      根本 知己
    • Publisher
      (株)オーム社
    • Related Report
      2007 Annual Research Report
  • [Book] 分子イメージング最前線-蛍光プローブが拓くライフサイエンスの未来(化学同人編集部編), 第4章:2光子顕微鏡によるin vivo可視化技術2007

    • Author(s)
      根本知己
    • Publisher
      (株)化学同人
    • Related Report
      2009 Final Research Report
  • [Book] 分子イメージング最前線: 蛍光プローブが拓くライフサイエンスの未来」第4章「2光子顕微鏡によるin vivo可視化技術2007

    • Author(s)
      根本 知己
    • Publisher
      化学同人
    • Related Report
      2007 Annual Research Report
  • [Remarks]

    • URL

      http://www.es.hokudai.ac.jp/labo/mcb/

    • Related Report
      2009 Final Research Report
  • [Remarks]

    • URL

      http://www.nips.ac.jp/tp/

    • Related Report
      2008 Annual Research Report
  • [Patent(Industrial Property Rights)] 生体内特定代謝物の迅速測定法と装置2009

    • Inventor(s)
      岸本拓哉, 大西通博, 根本知己, 鍋倉淳一
    • Industrial Property Rights Holder
      ソニー株式会社、自然科学研究機構
    • Filing Date
      2009-12-17
    • Related Report
      2009 Final Research Report
  • [Patent(Industrial Property Rights)] 生体内特定代謝物の迅速測定法と装置2009

    • Inventor(s)
      岸本拓哉、大西通博、根本知己、鍋倉淳一
    • Industrial Property Rights Holder
      ソニー株式会社・自然科学機構
    • Industrial Property Number
      2009-286695
    • Filing Date
      2009-12-17
    • Related Report
      2009 Annual Research Report

URL: 

Published: 2005-04-01   Modified: 2018-03-28  

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