Molecular structure and early events during signal transduction of LOV-proteins regulating movements of plants
Project/Area Number |
17084008
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Research Category |
Grant-in-Aid for Scientific Research on Priority Areas
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Allocation Type | Single-year Grants |
Review Section |
Biological Sciences
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Research Institution | Osaka Prefecture University |
Principal Investigator |
TOKUTOMI Satoru Osaka Prefecture University, 理学系研究科, 教授 (90142009)
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Co-Investigator(Kenkyū-buntansha) |
YOSHIHARA Shizue 大阪府立大学, 理学系研究科, 助教 (20382236)
|
Project Period (FY) |
2005 – 2009
|
Project Status |
Completed (Fiscal Year 2009)
|
Budget Amount *help |
¥86,300,000 (Direct Cost: ¥86,300,000)
Fiscal Year 2009: ¥16,800,000 (Direct Cost: ¥16,800,000)
Fiscal Year 2008: ¥16,800,000 (Direct Cost: ¥16,800,000)
Fiscal Year 2007: ¥16,800,000 (Direct Cost: ¥16,800,000)
Fiscal Year 2006: ¥16,800,000 (Direct Cost: ¥16,800,000)
Fiscal Year 2005: ¥19,100,000 (Direct Cost: ¥19,100,000)
|
Keywords | 植物 / LOV光受容体 / フォトトロピン / 分子構造 / 光反応 / シグナル伝達 / 青色光受容 / フラビンタンパク質 / LOVドメイン / 結晶構造 |
Research Abstract |
Phototropin (phot) is a blue light receptor for phototropism, chloroplast relocation movements, stomatal opening, etc that are involved in fine-tuning of the efficiency of photosynthesis in plants. A phot molecule has two photoreceptive domains named LOV1 and LOV2 and is thought to be a light-regulated protein kinase. The present study aimed to solve the molecular structure of the two LOV and the kinase domains and analyze the molecular mechanism that regulates the kinase activity by light, and gathered many useful information.
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Report
(6 results)
Research Products
(174 results)
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[Journal Article] Oligomeric State-Dependent Conformational Change of the BLUF ProteinTePixD (Tll0078)2009
Author(s)
Tanaka, K., Nakasone, Y., Okajima, K., Ikeuchi, M., Tokutomi, S., Terazima, M.
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Journal Title
J. Mol. Biol. 386巻
Pages: 1290-1230
Related Report
Peer Reviewed
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[Journal Article] Time-Resolved FT-IR Study on Photoadduct Formation and Secondary Structural Changes within the Phototropin LOV Domain2009
Author(s)
Pfeifer, A., Majerus, T., Zikihara, K., Matsuoka, D., Tokutomi, S., Heberle, J., Kottke, T.
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Journal Title
BiophysJ. 96巻
Pages: 1462-1470
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Peer Reviewed
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[Journal Article] Oligomeric State-Dependent Conformational Change of the BLUF Protein TePixD (T110078)2009
Author(s)
Tanaka, K., Nakasone, Y., Okajima, K., Ikeuchi, M., Tokutomi, S., Terazima, M.
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Journal Title
J.Mol.Biol. 386
Pages: 1290-1230
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Peer Reviewed
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[Journal Article] Time-Resolved FT-IR Study on Photoadduct Formation and Secondary Structural Changes within the Phototropin LOV Domain.2009
Author(s)
Pfeifer, A., Majerus, T., Zikihara, K., Matsuoka, D., Tokutomi, S., Heberle, J., Kottke, T.
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Journal Title
Biophys J 96
Pages: 1462-1470
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Peer Reviewed
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[Journal Article] Time-Resolved FT-IR Study on Photoadduct Formation and Secondary Structural Changes within the Phototropin LOV Doitain2009
Author(s)
Pfeifer, A., Majerus, T., Zikihara, K., Matsuoka, D., Tokutomi, S., Heberle, J and Kottke, T
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Journal Title
Biophys J 96
Pages: 1462-1470
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Peer Reviewed
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[Journal Article] Stability of Dimer and Domain-Domain Interaction of Arabidopsis Phototropin 1 LOV22008
Author(s)
Nakasone, Y., Eitoku, T., Zikihara, K., Matsuoka, D., Tokutomi, S., Terazima, M.
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Journal Title
J. Mol. Biol. 373巻
Pages: 904-913
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Peer Reviewed
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[Journal Article] Crystallization and preliminary X-ray diffraction analysis of the LOV1 domains of phototropin 1 and 2 from Arabidopsis thaliana2008
Author(s)
Nakasako, M., Hirata, M., Shimizu, N., Hosokawa, S., Matsuoka, D., Oka, T., Yamamoto, M., Tokutomi, S.
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Journal Title
Acta Crystallogr. Sect F. 64巻
Pages: 617-621
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Peer Reviewed
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[Journal Article] Blue light diminishes interaction of PAS/LOV proteins, putative blue light receptors in Arabidopsisthaliana, with their interacting partners2008
Author(s)
Ogura, Y., Komatsu, A., Zikihara, K., Nanjo, T., Tokutomi, S., Wada, M. andKiyosue, T.
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Journal Title
J Plant Research. 121巻
Pages: 97-105
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Peer Reviewed
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[Journal Article] Stability of Dimer and Domam-Doniam InieraCtioh of Arabidopsis Photo trapm 1 LOV22008
Author(s)
Nakasone, Y., Eitoku, T., Zikihara, K., Matsuoka, D., Tokutomi, S., and Terazima, M
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Journal Title
J. Mol. Biol 373
Pages: 904-913
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Peer Reviewed
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[Journal Article] Crystallization and preliminary X-ray diffraction analysis of the LOV1 domains of phototropin 1 and 2 from Arabidopsis, thaliana2008
Author(s)
Nakasalui, M., Hirata, M., Shunizu, N., Hosokawa, S., Matsuoka, D., Oka, T., Yamamoto, M. and Tokutomi, S
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Journal Title
Acta Crystallogr Sect F 64
Pages: 617-621
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Peer Reviewed
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[Journal Article] Blue light dimuushes interaction of PAS/LOV proteins, putativeblue light receptors in Arabidopsis thahana, with their mteracting partners2008
Author(s)
Ogura, Y., Komatsu, A., Zikihara, K., Nanjo, T., Tokutomi, S., Wada, M. and Kiyosue, T
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Journal Title
J Plant Research 121
Pages: 97-195
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Peer Reviewed
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[Journal Article] Blue light diminishes interaction of PAS/LOV proteins,putative blue light receptors in Arabidopsis thaliana,with their interacting partners.2008
Author(s)
Ogura, Y., Komatsu, A, Zikihara, K., Nanjo, T., Tokutomi.S., Wada, M.and Kiyosue, T
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Journal Title
J Plant Research 121
Pages: 97-105
NAID
Related Report
Peer Reviewed
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[Journal Article] Heterogeneous Environment of the S-H Group of Cys966 near the Flavin Chromophore in the LOV2 Domain of AdiantumNeochrome12007
Author(s)
Sato, Y., Nabeno, M., Iwata, T., Tokutomi, S., Sakurai, M., Kandori, N.
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Journal Title
Biochemistry 46巻
Pages: 1025-65
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Peer Reviewed
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[Journal Article] Photochemical intermediates of Arabidopsis phototropin 2 LOV2 associated with conformational changes.2007
Author(s)
Eitoku, T., Nakasone, Y., Zikihara, K., Matsuoka, D., Tokutomi, S., Terazima, M.
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Journal Title
J. Mol. Biol. 371巻
Pages: 1290-303
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Peer Reviewed
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[Journal Article] Heterogeneous Environment of the S-H Group of Cys 966 near the Flavin Chromophore in the LOV2 Domain of Adiantum Neochromel.2007
Author(s)
Sato, Y., Nabeno, M., Iwata, T., Tokutomi, S., Sakurai, M.and Kandori, N.
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Journal Title
Biochemistry 46
Pages: 1025-1065
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Peer Reviewed
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[Journal Article] Photochemical intermediates of Arabidopsis phototropin 2LOV2 associated with conformational changes.2007
Author(s)
Eitoku, T., Nakasone, Y., Zikihara, K., Matsuoka, D., Tokutomi, S.and Terazima, M.
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Journal Title
J.Mol.Biol. 371
Pages: 1290-303
Related Report
Peer Reviewed
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[Journal Article] Reconstitution of blue/greenreversible photoconversion of a cyanobacterial photoreceptor, PixJ1, in phycocyanobilin producing E. coli2006
Author(s)
Yoshihara, S., Shimada, T., Matsuoka, D., Zikihara, K., Kohchi, T., Tokutomi, S.
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Journal Title
Biochyemistry 45巻
Pages: 3783-3792
Related Report
Peer Reviewed
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[Journal Article] Photoreaction cycle of LOV domain in FKF1 determined by low-temperatureabsorption spectroscopy2006
Author(s)
Zikihara, K., Iwata, T., Matsuoka, D., Kandori, H., Todo, T., Tokutomi, S.
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Journal Title
Biochemistry 36巻
Pages: 10828-10837
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Peer Reviewed
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[Journal Article] Three Putative Photosensory LOV Domains with Distinct Biochemical Properties from the Filamentous Cyanobacterium Anabaena sp. PCC 7120.2006
Author(s)
Narikawa, R., Zikihara, K., Okajima, K., Ochiai, Y., Katayama, M., Shichida, Y., Tokutomi, S., Ikeuchi, M.
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Journal Title
Photochem. Photobiol 82巻
Pages: 1627-1633
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Peer Reviewed
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[Journal Article] Identification ofthe C=O stretching vibrations of FMN and peptide backbone by 13C-labeling of the LOV2 domain of Adiantum phytochrome32006
Author(s)
Iwata, T., NozakI, D., Sato, Y., Sato, K., Nishina, Y., Shiga, K., Tokutomi, S., Kandori, H.
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Journal Title
Biochemistry 45巻
Pages: 15384-15391
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Peer Reviewed
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[Journal Article] Identification of the C=O stretching vibrations of FMN and peptide backbone by 13C-labeling of the LOV2 domain of Adiantum phytochrome32006
Author(s)
Iwata, T., NozakI, D., Sato, Y., Sato, K., Nishina, Y., Shiga, K., Tokutomi, S., Kandori, H.
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Journal Title
Biochemistry 45
Pages: 15384-15391
Related Report
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[Journal Article] Three Putative Photosensory LOV Domains with Distinct Biochemical Properties from the Filamentous Cyanobacterium Anabaena sp. PCC 7120.2006
Author(s)
Narikawa, R., Zikihara, K., Okajima, K., Ochiai, Y., Katayama, M., Shichida, Y., Tokutomi, S., Ikeuchi, M.
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Journal Title
Photochem. Photobiol. 82
Pages: 1627-1633
Related Report
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[Journal Article] Photoreaction cycle of LOV domain in FKF1 determined by low-temperature absorption spectroscopy.2006
Author(s)
Zikihara, K., Iwata, T., MatsuokaD., Kandori, H., Todo, T., Tokutomi, S.
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Journal Title
Biochemistry 36
Pages: 10828-10837
Related Report
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[Journal Article] Reconstitution of blue/green reversible photoconversion of a cyanobacterial photoreceptor, PixJ1, in phycocyanobilin producing E. coli.2006
Author(s)
Yoshihara, S., Shimada, T., Matsuoka, D., Zikihara, K., Kohchi, T., Tokutomi, S.
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Journal Title
Biochyemistry 45
Pages: 3783-3792
Related Report
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[Journal Article] Reconstitution of blue/green reversible photoconversion of a cyanobacterialphotoreceptor, PixJ1, in phycocyanobilin producing E.coli.2006
Author(s)
Yoshihara, S., Shimada, T., Matsuoka, D., Zikihara, K., Kohchi, T., Tokutomi, S.
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Journal Title
Biochemistry 45
Pages: 3775-3784
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[Presentation] Involvement of Arg427 in the light-signal transduction of Arabidopsis phototropin2, a blue-light photoreceptor2009
Author(s)
Zikihara, K., Katsura, H., Oyamatsu, S., Sakurai, M., Tokutomi, S.
Organizer
第47回日本生物物理学会
Place of Presentation
徳島
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[Presentation] Temperature-dependent reaction efficiency of phototropin and possible function of temperature sensor2009
Author(s)
Nakasone, Y., Zikihara, K., Matsuoka, D., Tokutomi, S., Suzuki, T., Nagatani, A., Terazima, M.
Organizer
第47回日本生物物理学会
Place of Presentation
徳島
Related Report
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[Presentation] Pressure effects on reaction dynamics of a photosensor protein TePixD2009
Author(s)
Kuroi.K., Tanaka, K., Nakasone, Y, Okajima, K., Ikeuchi, M., Tokutomi, S., Terazima, M.
Organizer
第47回日本生物物理学会
Place of Presentation
徳島
Related Report
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[Presentation] Light-induced structural changes of the LOV2 domain studied by FTIR spectroscopy of isotope labeled samples2008
Author(s)
Koyama, T., Yamamoto, A., Sato, Y., Iwata, T., Matuoka, D., Tokutomi, S.
Organizer
日本生物物理学会第46回年会
Place of Presentation
福岡
Related Report
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[Presentation] Structural basis for the photoreception of Arabidpsis phototropin2008
Author(s)
Tokutomi. S., Zikihara, K., Okajima, K,, Katsura, H., Yoshihara, S., Nakasako M
Organizer
15th Anniversary Meeting for Korean Society of Photoscience, Symposium
Place of Presentation
Korea, Jeju
Related Report
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[Presentation] Comparative studies of light-induced structural changes of the LOV2 domain and the Jαhelix among phototropins.2008
Author(s)
Koyama, T., Yamamoto, A., Iwata, T., Sato, Y., Matsuoka, D., Tokutomi S., Kandori, H.
Organizer
The 5th Open Workshop for "Chemistry of Biological Processes Created by Water and Biomolecules
Place of Presentation
Nara, Japan
Related Report
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[Presentation] Time-resolved thermodynamics on photochemical intermediates of phototropin associated with the conformational change2008
Author(s)
Eitoku, T., Nakasone, Y., Zikihara, K., Matsuoka, D., Tokutomi, S., Terazima, M.
Organizer
Gordon Research Conference: Photosensory Receptors & Signal Transduction
Place of Presentation
Ventura, CA, USA
Related Report
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[Presentation] Structural basis for the photoreception of Arabidpsis phototropin2008
Author(s)
Tokutomi S., Zikihara, K., Okajima, K., Katsura, H., Yoshihara, S., Nakasako, M
Organizer
15^<th> Anniversary Meetmg for Korean Society of Photscience
Place of Presentation
Korea, Jeju
Related Report
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[Presentation] 同位体標識試料を用いたLOV2ドメインの光誘起構造変化の赤外分光解析2008
Author(s)
Koyama, T, Yamamoto, A, Sato, Y, Iwata, T, Matuoka, D and Tokutomi, S
Organizer
日本生物物理学会第46回年会
Place of Presentation
福岡
Related Report
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[Presentation] 植物が有ずるLOVドメインの光反応ダイナミクス2008
Author(s)
Nakasone, Y, Eitoiu, T, Zukihara, K, Matsuoka, D, Tokutomi, S and-Terazima, M
Organizer
日本生物物理学会第46回年会
Place of Presentation
福岡
Related Report
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[Presentation] Comparatrve studres of light-induced structural changes of the LOV2 domain and the Jα helix among phototropins2008
Author(s)
Koyama, T, Yamamoto, A., Iwata, T., Sato, Y, Matsuoka, D., Tokutomi S.and Kandori, H
Organizer
The 5th Open Workshop for "Chemistxy of Biological Processes Created by Water and Biomolecules"
Place of Presentation
Nara,Japan
Related Report
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[Presentation] Time-resolved thermodynamics on photochemical intermediates of phototropin associated with the conformational change2008
Author(s)
Eitoku, T., Nakasone, Y., Zikihara, K., Matuoka, D., Tokutomi, S.&Terazima, M
Organizer
Gordon Research Conference:Photosensory Receptors&Signal Transduction
Place of Presentation
Ventura,CA,USA
Related Report
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[Presentation] Time-resolved thermaodynamics-on photochemical intermediates of phototropin associated with the conformational change2008
Author(s)
Nakasone, Y., Eitoku, T., Zikihara, K., Matuoka, D., Tokutomi, S.&Yerazima, M
Organizer
Gordon Research Conference:Photosensory Receptors&Signal Transduction
Place of Presentation
Ventura,CA,USA
Related Report
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[Presentation] Equiribum Between monomer and dimer forms of phototropin1 LOV2 domain2006
Author(s)
Nakasone, T., Eitoku, T., Matsuoka, D., Tokutomi, S., Terazima, M.
Organizer
5th East Asian Biophysical Symposium & 44th Annual Meeting of Biophysical Society of Japan
Place of Presentation
Okinawa, Japan
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