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Deep-sea deployment of a Microfabricated In Situ Gene Analysis System and Its Functional Sophistication

Research Project

Project/Area Number 17106012
Research Category

Grant-in-Aid for Scientific Research (S)

Allocation TypeSingle-year Grants
Research Field Naval and maritime engineering
Research InstitutionThe University of Tokyo

Principal Investigator

FUJII Teruo  The University of Tokyo, 生産技術研究所, 教授 (30251474)

Co-Investigator(Kenkyū-buntansha) YAMAMOTO Takatoki  東京大学, 生産技術研究所, 助教 (20322688)
FUKUBA Tatsuhiro  東京大学, 生産技術研究所, 特任准教授 (80401272)
Project Period (FY) 2005 – 2009
Project Status Completed (Fiscal Year 2009)
Budget Amount *help
¥91,260,000 (Direct Cost: ¥70,200,000、Indirect Cost: ¥21,060,000)
Fiscal Year 2009: ¥14,430,000 (Direct Cost: ¥11,100,000、Indirect Cost: ¥3,330,000)
Fiscal Year 2008: ¥17,160,000 (Direct Cost: ¥13,200,000、Indirect Cost: ¥3,960,000)
Fiscal Year 2007: ¥22,100,000 (Direct Cost: ¥17,000,000、Indirect Cost: ¥5,100,000)
Fiscal Year 2006: ¥19,370,000 (Direct Cost: ¥14,900,000、Indirect Cost: ¥4,470,000)
Fiscal Year 2005: ¥18,200,000 (Direct Cost: ¥14,000,000、Indirect Cost: ¥4,200,000)
Keywordsマイクロ流体デバイス / 現場分析装置 / 遺伝子解析 / 深海 / PCR / DNA精製 / 微生物 / ATP / IISA-Gene / IISA-ATP
Research Abstract

In this study, we developed an in situ gene analysis system that can be applied to oceanography. The system was successfully deployed in deep-sea environments to analyze microbial communities. By utilizing microfluidic devices, sample pre-treatment function was integrated. In situ microbial activity assay method was also established in this study. Corroborative work was carried out with Monterey Bay Aquarium Research Institute (MBARI) and it resulted in a successful evaluation of the system for red-tide plankton monitoring.

Report

(6 results)
  • 2009 Annual Research Report   Final Research Report ( PDF )
  • 2008 Annual Research Report
  • 2007 Annual Research Report
  • 2006 Annual Research Report
  • 2005 Annual Research Report
  • Research Products

    (59 results)

All 2009 2008 2007 2006 2005 Other

All Journal Article (20 results) (of which Peer Reviewed: 3 results) Presentation (33 results) Book (2 results) Remarks (4 results)

  • [Journal Article] Design Optimization and Evaluation of a Bioluminescence Detection Part on a Microfluidic Device for in situ ATP Quantification2009

    • Author(s)
      Y. Aoki, T. Fukuba, T. Yamamoto, T. Fujii
    • Journal Title

      IEEJ Trans.SM 129

      Pages: 73-76

    • NAID

      10024776610

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Design Optimization and Evaluation of a Bioluminescence Detection Part on a Microfluidic Device for in situ ATP Quantification2009

    • Author(s)
      Y. Aoki, ほか
    • Journal Title

      IEEJ Transactions on Electrical and Electronic Engineering, Sensors and Micromachines 129

      Pages: 73-76

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 海底表層でのバイオマーカを利用したメタン漏洩検知技術の開発2008

    • Author(s)
      帆秋利洋, 福場辰洋, 藤井輝夫, 布施博之
    • Journal Title

      海洋と生物 177

      Pages: 505-515

    • Related Report
      2009 Final Research Report
  • [Journal Article] ガス漏洩モニタリング (間接検出法 : バイオマーカー利用モニタリング)―遺伝子マーカーによるメタン漏洩の原位置検出技術の開発―2008

    • Author(s)
      帆秋利洋, 沖田紀子, 布施博之, 福場辰洋, 藤井輝夫, 鋤崎俊二, 天石文, 片山美津瑠, 吉田光毅, 安田博和, 藤原靖
    • Journal Title

      月刊海洋 40

      Pages: 106-116

    • Related Report
      2009 Final Research Report
  • [Journal Article] 海底表層でのバイオマーカを利用したメタン漏洩検知技術の開発2008

    • Author(s)
      帆秋利洋, ほか
    • Journal Title

      海洋と生物 177

      Pages: 505-515

    • Related Report
      2008 Annual Research Report
  • [Journal Article] ガス漏洩モニタリング(間接検出法:バイオマーカー利用モニタリング)-遺伝子マーカーによるメタン漏洩の原位置検出技術の開発-2008

    • Author(s)
      帆秋利洋, ほか
    • Journal Title

      月刊海洋 40

      Pages: 106-116

    • Related Report
      2007 Annual Research Report
  • [Journal Article] 現場計測技術開発と海洋動物研究を目的とした鳩間海丘調査 (NT06-14航海報告)2007

    • Author(s)
      藤井 輝夫 他
    • Journal Title

      第23回しんかいシンポジウム (Blue Earth'07) 予稿集

      Pages: 64-65

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Development of an Integrated In Situ Analyzer for Quantitative Analysis of Microbial ATP in Aquatic Environments2007

    • Author(s)
      T.Fukuba et al.
    • Journal Title

      Proceedings of International Symposium on Underwater Technology 2007 (In press)

    • Related Report
      2006 Annual Research Report
  • [Journal Article] 現場型遺伝子解析装置"IISA-Gene"の開発2006

    • Author(s)
      福場辰洋, 宮地輝光, 山本貴富喜, 藤井輝夫
    • Journal Title

      月刊海洋 38

      Pages: 871-876

    • Related Report
      2009 Final Research Report
  • [Journal Article] 海洋における現場計測分析技術の新展開―総説―2006

    • Author(s)
      藤井輝夫
    • Journal Title

      月刊海洋 38

      Pages: 825-830

    • Related Report
      2009 Final Research Report
  • [Journal Article] 極限環境用マイクロ現場分析装置"IISA"シリーズの開発と評価2006

    • Author(s)
      福場 辰洋 他
    • Journal Title

      第13回化学とマイクロ・ナノシステム研究会 (13^<th>CHEMINAS) 予稿集

    • Related Report
      2006 Annual Research Report
  • [Journal Article] 海洋における現場計測分析技術の新展開-総説-2006

    • Author(s)
      藤井 輝夫
    • Journal Title

      月刊海洋 38

      Pages: 825-830

    • Related Report
      2006 Annual Research Report
  • [Journal Article] 現場型遺伝子解析装置"IISA-Gene"の開発2006

    • Author(s)
      福場 辰洋 他
    • Journal Title

      月刊海洋 38

      Pages: 871-876

    • Related Report
      2006 Annual Research Report
  • [Journal Article] マイクロ流体デバイスを用いた集積化現場計測「IISA」の開発2006

    • Author(s)
      福場 辰洋 他
    • Journal Title

      日本船舶海洋工学会講演会論文集 3

      Pages: 31-34

    • Related Report
      2006 Annual Research Report
  • [Journal Article] 深海熱水地帯における現場複合計測技術の開発- 熱水プルームマッピングと現場遺伝子解析の試み2006

    • Author(s)
      藤井輝夫 他
    • Journal Title

      第22回しんかいシンポジウム(Blue Earth'06)予稿集

      Pages: 60-61

    • Related Report
      2005 Annual Research Report
  • [Journal Article] マイクロ加工技術を用いた現場型微生物遺伝子解析装置(IISA-Gene)の開発と実海域における試験運用2006

    • Author(s)
      福場辰洋 他
    • Journal Title

      第22回しんかいシンポジウム(Blue Earth'06)予稿集

      Pages: 258-259

    • Related Report
      2005 Annual Research Report
  • [Journal Article] 極限環境微生物学のための現場型微生物遺伝子解析装置の開発2005

    • Author(s)
      福場辰洋, 山本貴富喜, 長沼毅, 藤井輝夫
    • Journal Title

      海の研究 14

      Pages: 361-368

    • NAID

      110003375616

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Processing and Analyzing Biological Samples in Microchannels2005

    • Author(s)
      T.Fujii
    • Journal Title

      Proceedings of 3rd International Conference on Microchannels and Minichannels ICMM2005

      Pages: 75083-75083

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Development of Miniaturized In situ Analysis Devices for Biological and Chemical Oceanography2005

    • Author(s)
      T.Fukuba et al.
    • Journal Title

      The 3rd Annual International IEEE EMBS Special Topic Conference on Microtechnologies in Medicine and Biology

      Pages: 56-59

    • Related Report
      2005 Annual Research Report
  • [Journal Article] 深海熱水地帯における化学及び生物活動に関する現場複合計測の試み2005

    • Author(s)
      福場辰洋 他
    • Journal Title

      2005年度日本地球化学会第52回年会講演要旨集

      Pages: 7-7

    • NAID

      130004592652

    • Related Report
      2005 Annual Research Report
  • [Presentation] Microbial Activity Assay in Deep-Sea Environment using a Microfluidic Device2009

    • Author(s)
      T. Fukuba, Y Aoki, T. Fujii
    • Organizer
      μTAS2009, Proceedings, (2010-2012)
    • Place of Presentation
      Jeju, Korea
    • Year and Date
      2009-11-05
    • Related Report
      2009 Final Research Report
  • [Presentation] Microbial Activity Assay in Deep-Sea Environment using a Microfluidic Device2009

    • Author(s)
      T.Fukuba, Y Aoki, T.Fujii
    • Organizer
      The 13^<th> International Conference on Miniaturized Systems for Chemistry and Life Sciences(μTAS2009)
    • Place of Presentation
      韓国・済州
    • Year and Date
      2009-11-05
    • Related Report
      2009 Annual Research Report
  • [Presentation] PCR Based DNA Detection in Deep-Sea Environment2009

    • Author(s)
      T. Fukuba, M. Hiraga, A. Takamatsu, T. Fujii
    • Organizer
      μTAS2009, Proceedings(1279-1281)
    • Place of Presentation
      Jeju, Korea
    • Year and Date
      2009-11-03
    • Related Report
      2009 Final Research Report
  • [Presentation] PCR Based DNA Detection in Deep-Sea Environment2009

    • Author(s)
      T.Fukuba, M.Hiraga, A.Takamatsu, T.Fujii
    • Organizer
      The 13^<th> International Conference on Miniaturized Systems for Chemistry and Life Sciences(μTAS2009)
    • Place of Presentation
      韓国・済州
    • Year and Date
      2009-11-03
    • Related Report
      2009 Annual Research Report
  • [Presentation] 海洋探索における最新マイクロ流体デバイスの利用と展望~深海フィールド型遺伝子解析装置の開発にみる~2009

    • Author(s)
      福場辰洋、藤井輝夫
    • Organizer
      第59回日本電気泳動学会シンポジウム
    • Place of Presentation
      東京・立教大学
    • Year and Date
      2009-05-29
    • Related Report
      2009 Annual Research Report
  • [Presentation] Development of Integrated ln Situ Analyzers and its Application to Oceanography2009

    • Author(s)
      T. Fukuba, ほか
    • Organizer
      PITTCON2009
    • Place of Presentation
      アメリカ・シカゴ
    • Year and Date
      2009-03-13
    • Related Report
      2008 Annual Research Report
  • [Presentation] Development of Integrated In Situ Analyzers and its Application to Oceanography2009

    • Author(s)
      T. Fukuba, C. Provin, Y. Aoki, K. Okamura, K. Shitashima, T. Yamamoto, T. Fujii
    • Organizer
      PITTCON2009, Abstruct, (2060-9P)
    • Place of Presentation
      Chicago, USA
    • Related Report
      2009 Final Research Report
  • [Presentation] Microfluidic in situ Biological and Chemical Sensing2008

    • Author(s)
      T. Fukuba, T. Fujii
    • Organizer
      7^<th> International Symposium for Subsurface Microbiology (ISSM), Proceedings, (182)
    • Place of Presentation
      Shizuoka, Japan
    • Year and Date
      2008-11-20
    • Related Report
      2009 Final Research Report
  • [Presentation] Micronuidic in situ Biologlcal and Chemical Sensing2008

    • Author(s)
      T. Fukuba, ほか
    • Organizer
      7th International Symposium for Subsurface Microbiology(ISSM)
    • Place of Presentation
      静岡
    • Year and Date
      2008-11-20
    • Related Report
      2008 Annual Research Report
  • [Presentation] 現場型ATP定量装置IISA-ATPの開発とマイクロ流路形状の検討2008

    • Author(s)
      Y. Aoki, ほか
    • Organizer
      第25回「センサ・マイクロマシンと応用システム」シンポジウム
    • Place of Presentation
      沖縄
    • Year and Date
      2008-10-23
    • Related Report
      2008 Annual Research Report
  • [Presentation] Simple Method for Quantitative PCR Using Flow-Through PCR Device2008

    • Author(s)
      T. Fukuba, M. Hiraga, A. Takamatsu, C. Provin, T. Yamamoto, T. Fujii
    • Organizer
      μTAS 2008, Proceedings, (1743-1745)
    • Place of Presentation
      San Diego, USA
    • Year and Date
      2008-10-16
    • Related Report
      2009 Final Research Report
  • [Presentation] Simple Method for Quantitative PCR Using FIow-Through PCR Device2008

    • Author(s)
      T. Fukuba, ほか
    • Organizer
      The 12th Internadonal Conference on Miniatuhzed Systems for Chemistry and Life Sciences(μTAS2008)
    • Place of Presentation
      アメリカ・サンディエゴ
    • Year and Date
      2008-10-16
    • Related Report
      2008 Annual Research Report
  • [Presentation] Flow-through PCR装置によるDNAの定量2008

    • Author(s)
      平賀雅隆, ほか
    • Organizer
      第17回化学とマイクロ・ナノシステム研究会(17th CHEMINAS)
    • Place of Presentation
      福岡
    • Year and Date
      2008-05-21
    • Related Report
      2008 Annual Research Report
  • [Presentation] 海洋底泥層における微生物遺伝子マーカーの探索と利用2008

    • Author(s)
      帆足利洋, ほか
    • Organizer
      平成20年度海洋理工学会春季大会
    • Place of Presentation
      東京
    • Year and Date
      2008-05-15
    • Related Report
      2008 Annual Research Report
  • [Presentation] マイクロ流体デバイスを応用した現場型遺伝子解析装置「IISA-Gene」によるバイオマーカ検2008

    • Author(s)
      福島辰洋, ほか
    • Organizer
      平成20年度海洋理工学会春季大会
    • Place of Presentation
      東京
    • Year and Date
      2008-05-15
    • Related Report
      2008 Annual Research Report
  • [Presentation] DNA markers associated with methane leakage from gas hydrates in the deep sea2008

    • Author(s)
      M. Katayama, ほか
    • Organizer
      OCEANS'08 MTS/IEEE KOBE-TECHNO-OCEAN'08
    • Place of Presentation
      神戸
    • Year and Date
      2008-04-10
    • Related Report
      2008 Annual Research Report
  • [Presentation] Development of"IISA-ATP"System for in situ Microbial Activity Assessment in Deep-sea2008

    • Author(s)
      Y. Aoki, ほか
    • Organizer
      OCEANS'08 MTS/IEEE KOBE-TECHN0-OCEAN'08
    • Place of Presentation
      神戸
    • Year and Date
      2008-04-10
    • Related Report
      2008 Annual Research Report
  • [Presentation] Flow-through PCR装置によるDNAの定量2008

    • Author(s)
      平賀雅隆, 福場辰洋, 山本貴富喜, 高松敦子, 藤井輝夫
    • Organizer
      第17回化学とマイクロ・ナノシステム研究会 (17th CHEMINAS)要旨集, (57)
    • Place of Presentation
      福岡
    • Related Report
      2009 Final Research Report
  • [Presentation] Development of "IISA-ATP" System for in situ Microbial Activity Assessment in Deep-sea Environment2008

    • Author(s)
      Y. Aoki, T. Fukuba, T. Yamamoto, T. Fujii
    • Organizer
      OCEANS'08 NITS/IEEE KOBE-TECHNO-OCEAN'08
    • Place of Presentation
      Kobe, Japan
    • Related Report
      2009 Final Research Report
  • [Presentation] Development of Integrated in situ Analyzers (IISA) for Oceanography Applications2007

    • Author(s)
      T. Fukuba, A. Miyaji, N. Fukuzawa, C. Provin, T. Yamamoto, L. Glutz, T. Okamoto, T. Fujii
    • Organizer
      μTAS 2007, Proceedings, (844-846)
    • Place of Presentation
      Paris, France
    • Year and Date
      2007-10-09
    • Related Report
      2009 Final Research Report
  • [Presentation] Development of Integrated in situ Analyzers (IISA) for Oceanography Applications2007

    • Author(s)
      T. Fukuba, ほか
    • Organizer
      Eleventh International Conference on Miniaturized Systems for Chemistry and Life Sciences (μTAS 2007)
    • Place of Presentation
      フランス・パリ
    • Year and Date
      2007-10-09
    • Related Report
      2007 Annual Research Report
  • [Presentation] 現場型ATP定量装置IISA-ATPの開発とマイクロ流路形状の検討2007

    • Author(s)
      青木優介, 福場辰洋, 山本富貴喜, 藤井輝夫
    • Organizer
      第25回センサ・マイクロマシンと応用システムシンポジウム要旨集, (103)
    • Place of Presentation
      東京
    • Year and Date
      2007-09-15
    • Related Report
      2009 Final Research Report
  • [Presentation] 現場型微生物遺伝子解析装置「IISA-Gene」の開発2007

    • Author(s)
      福場辰洋, ほか
    • Organizer
      東京大学生命科学ネットワークシンポジウム2007
    • Place of Presentation
      東京
    • Year and Date
      2007-09-15
    • Related Report
      2007 Annual Research Report
  • [Presentation] 現場型微生物活性分析装置"IISA-ATP"の開発2007

    • Author(s)
      青木優介, ほか
    • Organizer
      東京大学生命科学ネットワークシンポジウム2007
    • Place of Presentation
      東京
    • Year and Date
      2007-09-15
    • Related Report
      2007 Annual Research Report
  • [Presentation] Microfluidic-Based in situ Biological and Chemical Sensing-Towards Integrated and Real-Time Measurements in Deep-sea-2007

    • Author(s)
      T. Fujii, T. Fukuba
    • Organizer
      International Symposium on Underwater Technology 2007, Proceedings, (210)
    • Place of Presentation
      Tokyo, Japan
    • Year and Date
      2007-04-19
    • Related Report
      2009 Final Research Report
  • [Presentation] Development of an Integrated In Situ Analyzer for Quantitative Aalysis of Microbial ATP in Aquatic Environments2007

    • Author(s)
      T. Fukuba, N. Fukuzawa, L. S. Glutz, A. Miyaji, T. Fujii
    • Organizer
      International Symposium on Underwater Technology 2007, Proceedings, (240-244)
    • Place of Presentation
      Tokyo, Japan
    • Year and Date
      2007-04-19
    • Related Report
      2009 Final Research Report
  • [Presentation] Development of an Integrated In Situ Analyzer for Quantitative Analysis of Microbial ATP in Aquatic Environments2007

    • Author(s)
      T. Fukuba, ほか
    • Organizer
      International Symposium on Underwater Technology 2007
    • Place of Presentation
      東京
    • Year and Date
      2007-04-19
    • Related Report
      2007 Annual Research Report
  • [Presentation] Microfluidic-Based in situ Biological and Chemical Sensing -Towards Integrated and Real-Time Measurements in Deep-sea-2007

    • Author(s)
      T. Fujii, ほか
    • Organizer
      International Symposium on Underwater Technology 2007
    • Place of Presentation
      東京
    • Year and Date
      2007-04-19
    • Related Report
      2007 Annual Research Report
  • [Presentation] Development and Evaluation of the Integrated In Situ Analyzer for Gene-"IISA-Gene" for Microbiology in Extreme Environments2006

    • Author(s)
      T. Fukuba, T. Fujii
    • Organizer
      2006 International Conference on Microtechnologies in Medicine and Biology, Proceedings, (184-187)
    • Place of Presentation
      Okinawa, Japan
    • Related Report
      2009 Final Research Report
  • [Presentation] Development of in situ ATP Quantitative Analysis System "IISA-ATP""2006

    • Author(s)
      N. Fukuzawa, T. Fukuba, T. Fujii
    • Organizer
      2006 Inter national Conference on Micro technologies in Medicine and Biology, Proceedings, (260-263)
    • Place of Presentation
      Okinawa, Japan
    • Related Report
      2009 Final Research Report
  • [Presentation] 深海熱水地帯における化学及び生物活動に関する現場複合計測の試み2005

    • Author(s)
      福場辰洋, 岡本拓士, 長沼毅, 金子亮, 林徹, 許正憲, 吉田尊雄, 岡村慶, 下島公紀, 前田義明, 小池祐一, 藤井輝夫
    • Organizer
      2005年度日本地球化学会第52回年会講演会要旨集 (7)
    • Place of Presentation
      沖縄
    • Year and Date
      2005-09-26
    • Related Report
      2009 Final Research Report
  • [Presentation] Development of miniaturized in situ analysis devices for biological and chemical oceanography2005

    • Author(s)
      T. Fukuba, A. Imhof, M. Matsunaga, N. Takagi, T. Yamamoto, K. Okamura, T. Naganuma, T. Fujii
    • Organizer
      Microtechnology in Medicine and Biology, 2005. 3rd IEEE/EMBS Special Topic Conference, Proceedings, (56-54)
    • Place of Presentation
      Hawaii, USA
    • Related Report
      2009 Final Research Report
  • [Presentation] Processing and Analyzing Biological Samples in Microchannels2005

    • Author(s)
      T. Fujii
    • Organizer
      3rd International Conference on Micro channels and Minichannels, Proceedings, (75083)
    • Place of Presentation
      ICMM
    • Related Report
      2009 Final Research Report
  • [Book] マイクロ流体デバイスの深海微生物探査への応用 (松永是、竹山春子監修「バイオテクノロジーシリーズ マリンメタゲノムの有効利用」)2009

    • Author(s)
      福場辰洋, 藤井輝夫
    • Publisher
      シーエムシー出版
    • Related Report
      2009 Final Research Report
  • [Book] バイオテクノロジーシリーズ マリンメタゲノムの有効利用(マイクロ流体デバイスの深海微生物探査への応用)2009

    • Author(s)
      松永是、竹山春子監修(福場辰洋、藤井輝夫)
    • Total Pages
      279
    • Publisher
      シーエムシー出版
    • Related Report
      2009 Annual Research Report
  • [Remarks]

    • URL

      http://www.microfluidics.iis.u-tokyo.ac.jp/index_j.html

    • Related Report
      2009 Final Research Report
  • [Remarks]

    • URL

      http://www.microfluidics.iis.u-tokyo.ac.jp/index_j.html

    • Related Report
      2009 Annual Research Report
  • [Remarks]

    • URL

      http://www.microfluidics.iis.u-tokyo.ac.jp/index_j.html

    • Related Report
      2008 Annual Research Report
  • [Remarks]

    • URL

      http://www.microfluidics.iis.u-tokyo.ac.jp/index_j.html

    • Related Report
      2007 Annual Research Report

URL: 

Published: 2005-04-01   Modified: 2016-04-21  

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