Elucidation of mechanism involved in suppressor activity of avirulent genes and its application for control of plant diseases.

• Project/Area Number: 17108001
• Research Category: Grant-in-Aid for Scientific Research (S)
• Allocation Type: Single-year Grants
• Research Field: Plant pathology
• Research Institution: Shizuoka University
• Principal Investigator: TSUYUMU Shinji Shizuoka University, 創造科学技術大学院, 教授 (30090541)
• Co-Investigator(Kenkyū-buntansha): TNAKA Shigeyasu 静岡大学, 創造科学技術大学院, 教授 (90146233) ; SHIOJIRI Nobuyosi 静岡大学, 理学部, 教授 (70162568) ; MURATA Takeomi 静岡大学, 農学部, 准教授 (30273171) ; TAKIKAWA Yuuichi 静岡大学, 農学部, 教授 (90163344) ; DOHRA Hideo 静岡大学, 遺伝子実験施設, 准教授 (10311705) ; HIRATA Hisae 静岡大学, 農学部, 准教授 (00432196) ; 原 正和 静岡大学, 農学部, 教授 (10293614)
• Project Period (FY): 2005 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥111,410,000 (Direct Cost: ¥85,700,000、Indirect Cost: ¥25,710,000); Fiscal Year 2009: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2008: ¥27,040,000 (Direct Cost: ¥20,800,000、Indirect Cost: ¥6,240,000); Fiscal Year 2007: ¥26,130,000 (Direct Cost: ¥20,100,000、Indirect Cost: ¥6,030,000); Fiscal Year 2006: ¥27,040,000 (Direct Cost: ¥20,800,000、Indirect Cost: ¥6,240,000); Fiscal Year 2005: ¥26,910,000 (Direct Cost: ¥20,700,000、Indirect Cost: ¥6,210,000)
• Keywords: 細菌 / 非病原力因子 / 遺伝子 / 病害抵抗性 / 非病原力遺伝子 / 植物病原細菌 / サプレッサー / 耐病性植物 / テロメレース / サプレッサー活性 / ペクチンメチルエステラーゼ / タイプIII分泌機構 / カンキツかいよう病菌 / エフェクター / 核局在性配列 / 病原サイクル / ガード分子 / ゲノミクス / プロテオミクス / 宿主認識 / CCoAMT / avrBs3 / pthファミリー / avrPto / avrRpt / avrB

Research Abstract

植物は、病原菌との遭遇に際し,抵抗性反応を誘導し、元来病気かからない。病気にかかるのは、例外的な出来事である。本研究では、この抵抗性誘導を抑制する病原菌側の因子を特定し、さらにこれに結合する植物の因子を発見し、この因子の抵抗性誘導分子への結合の特異性により、抵抗性反応のオン,オフが決定されることを明らかにし、これらの因子の改変で抵抗性反応を常にオンにすることによる新しい植物病害防除の戦略を示すことが出来た.

Research Products

• [Journal Article] A TonB-dependent transducer is responsible for regulation of pathogenicity-related genes in Xanthomonas axonopodis pv. citri. (2010)
  • Author(s): L. Q Aini, H Hirata, S. Tsuyumu
  • Journal Title: J. Gen. Plant Pathol 76 Pages: 132-142
  • NAID: 10029719320
  • Peer Reviewed
• [Journal Article] SlyA, a MarR family transcriptional regulator, is essential for virulence in Dickeya dadantii 3937 (2009)
  • Author(s): M.M. Haque, M.S. Kabir, L.Q. Aini, H. Hirata, S. Tsuyumu
  • Journal Title: J. Bacteriol 191 Pages: 5409-5418
  • Peer Reviewed
• [Journal Article] Initial Intracellular proteome profile of Aspergillus niger biofilms. (2009)
  • Author(s): G.K. Villena, L. Venkatesh, A. Yamazaki, S. Tsuyumu, M. Gutierrez-Correa
  • Journal Title: Bioresource Technol 16(1) Pages: 101-108
  • Peer Reviewed
• [Journal Article] Self-regulation of a new pathogenicity-related gene encoding. leucine-rich protein LrpX inXanthomonas oryzae pv. oryza. (2009)
  • Author(s): Md. R. Islum, H. Hirata, S. Tsuge, S. Tsuyumu
  • Journal Title: J. Gen. Plant Pathol 75 Pages: 56-65
  • Peer Reviewed
• [Journal Article] A leucine-rich protein, LrpX, is a new regulator of hrp genes in Xanthomonas oryzae pv. oryzae. (2009)
  • Author(s): Md. R. Islum, Md. S. Kabir, H. Hirata, S. Tsuge, S. Tsuyumu
  • Journal Title: J. Gen. Plant Pathol 75 Pages: 66-70
  • NAID: 10029717377
  • Peer Reviewed
• [Journal Article] Differential gene expression of some lignocellulolytic enzymes in Aspergillus niger biofilms. (2009)
  • Author(s): G.K. Villena, T. Fujikawa, S. Tsuyumu, M. Gutierrez-Correa
  • Journal Title: Rev. Peru Biol 15(2) Pages: 97-102
  • Peer Reviewed
• [Journal Article] Analyses of the genes involved in disease development to construct disease-resistant plants by genetic engineering. (2009)
  • Author(s): S. Tsuyumu, H. Hirata
  • Journal Title: J. Automation, Mobile Robotics and Intelligent Systems (JAMRIS) 3(4) Pages: 84-86
  • Peer Reviewed
• [Journal Article] SlyA, a MarR family transcriptional regulator, is essential for virulence in Dickeya dadantii 3937 (2009)
  • Author(s): M.M.Haque, M.S.Kabir, L.Q.Aini, H.Hirata, S.Tsuyumu
  • Journal Title: J.Bacteriol. 191 Pages: 5409-5418
  • Peer Reviewed
• [Journal Article] Initial Intracellular proteome profile of Aspergillus niger biofilms (2009)
  • Author(s): G.K.Villena, L.Venkatesh, A.Yamazaki, S.Tsuyumu, M.Gutierrez-Correa
  • Journal Title: Bioresource Technol. 16 Pages: 101-108
  • Peer Reviewed
• [Journal Article] Self-regulation of a new pathogenicity-related gene encoding. leucine-rich protein LrpX in Xanthomonas oryzae pv. oryza (2009)
  • Author(s): Md.R.Islum, H.Hirata, S.Tsuge, S.Tsuyumu
  • Journal Title: J.Gen.Plant Pathol. 75 Pages: 56-65
  • NAID: 10029717323
  • Peer Reviewed
• [Journal Article] Self-regulation of a new pathogenicity-related gene encoding leucine-rich protein LrpX in Xanthomonas oryzae pv. oryzae. (2009)
  • Author(s): Md. R. Islum, H. Hirata, S. Tsuge, S. Tsuvumu
  • Journal Title: J. Gen. Plant Pathol. 75 Pages: 56-65
  • NAID: 10029717323
  • Peer Reviewed
• [Journal Article] A leucine-rich protein, LrpX, is a new regulator of hrp genes in Xanthomonas oryzae pv. oryzae. (2009)
  • Author(s): Md. R. Islum, Md. S. Kabir, H. Hirata. S. Tsuge, S. Tsuvumu
  • Journal Title: J. Gen. Plant Pathol. 75 Pages: 66-71
  • NAID: 10029717377
  • Peer Reviewed
• [Journal Article] 植物は病気にかからない(Plants are basically resistant to any disease) (2008)
  • Author(s): 露無慎二, 藤川貴史, 駒井恵子, 木村幸, 小松節子, 平田久笑, 本当は
  • Journal Title: 土と微生物(Soil Microorganisms) 62(2) Pages: 102-105
  • Peer Reviewed
• [Journal Article] Type III regulators HrpG and HrpXct control synthesis of alpha-amylase, which is involved in in planta multiplication of Xanthomonas axonopodis pv. citri. (2008)
  • Author(s): H. Yamazaki, H. Hirata, S. Tsuyumu
  • Journal Title: J. Gen. Plant Pathol 74(3) Pages: 254-257
  • Peer Reviewed
• [Journal Article] HrpG regulates typeII secretory proteins in Xanthomonas axonopodis pv. citri. (2008)
  • Author(s): H. Yamazaki, H. Hirata, S. Tsuyumu
  • Journal Title: J. Gen. Plant Pathol 74 Pages: 138-150
  • NAID: 10029715787
  • Peer Reviewed
• [Journal Article] Genome sequence and rapid evolution o the rice pathogen Xanthomonas oryzae v. oyzae PXO99A. (2008)
  • Author(s): Salzberg L, et al., H.Hirtata, S.Tsuyumu
  • Journal Title: BMC Genomics 9 Pages: 204-204
  • Peer Reviewed
• [Journal Article] Type III regulators HrpG and HrpXct control synthesis of alpha-amylase, which is involved in in planta multiplication of Xanthomonas axonopodis pv. citri. (2008)
  • Author(s): H. Yamazaki, H. Hirata, S. Tsuvumu
  • Journal Title: J. Genlant Pathol. 74 Pages: 254-257
  • Peer Reviewed
• [Journal Article] HrpG regulates Type II secretion proteins in Xanthomonas campestris pv. citri (2008)
  • Author(s): H. Yamazaki, H. Hirata, S. Tsuyumu
  • Journal Title: J. Gen. Plant Pathol. 74 Pages: 258-271
  • Peer Reviewed
• [Journal Article] HrpG and HrpXct,type III regulators,control synthesis of α-amylase that is responsible for in planta multiplication of Xanthomonas axon opodis pv. citri (2008)
  • Author(s): H. Yamazaki, H. Hirata and S. Tsuyumu
  • Journal Title: J.Gen.Plant Pathol 74(印刷中)
  • Peer Reviewed
• [Journal Article] HrpG regulates typeII secretory proteins in Xanthomonas axonopodis pv.citri (2008)
  • Author(s): H. Yamazaki, H. Hirata and S. Tsuyumu
  • Journal Title: J.Gen.Plant Pathol 74 Pages: 138-150
  • Peer Reviewed
• [Journal Article] A sugar transporter (MfsX) is also required by Dickeya dadantiti 3937 for in planta fitness. (2007)
  • Author(s): T. Joko, H. Hirata, S. Tsuyumu
  • Journal Title: J. Gen. Plant Pathol 73 Pages: 274-280
  • Peer Reviewed
• [Journal Article] Sugar transporter (MfsX) of the major facilitator superfamily is required for flagella-mediated pathogenesis in Dickeya dadantiti 3937. (2007)
  • Author(s): T. Joko, H. Hirata, S. Tsuyumu
  • Journal Title: J. Gen. Plant Pathol 73 Pages: 266-273
  • Peer Reviewed
• [Journal Article] A pthA homolog from Xanthomonas axonopodis pv. citri responsible for host-specific suppression of virulence. (2007)
  • Author(s): H. Shiotani, T. Fujikawa, H. Ishihara, S. Tsuyumu, K. Ozaki
  • Journal Title: J. Bacteriol 189(8) Pages: 3271-3279
  • Peer Reviewed
• [Journal Article] Proteome analysis of the carbonate insoluble outer membrane fraction of the soft-rot pathogen Dickeya dadantii (syn. Erwinia chrysanthemi) Strain 3937. (2007)
  • Author(s): L. Babujjee, V. Balakrishnan, A. Yamazaki, S. Tsuyumu
  • Journal Title: J. Proteome Res 6(1) Pages: 62-69
  • Peer Reviewed
• [Journal Article] Proteome analysis of the carbonate insoluble outer membrane fraction of the soft-rot pathogen Dickeya dadantii(syn.Erwinia chrysanthemi)Strain 3937 (2007)
  • Author(s): L. Babujjee, V. Balakrishnan, A. Yamazaki, S. Tsuyumu
  • Journal Title: J.Proteome Res 6 Pages: 62-69
  • Peer Reviewed
• [Journal Article] Proteome analysis of the carbonate insoluble outer membrane fraction of the soft-rot pathogen Dickeya dadantii (syn. Erwinia chrysanthemi) Strain 3937. (2007)
  • Author(s): L.Babujjee, V.Balakrishnan, A.Yamazaki, S.Tsuyumu
  • Journal Title: J. Proteome Res. 6(1) Pages: 62-69
• [Journal Article] Bacterial Lipopolysaccharides induce defense responses associated with programmed cell death in rice cells. (2006)
  • Author(s): Y. Desaki, ら, 4番目S. Tsuyumu
  • Journal Title: Plant Cell Physiol 47 Pages: 1530-1540
  • NAID: 10018652435
  • Peer Reviewed
• [Journal Article] The Erwinia chrysanthemi 3937 PhoQ sensor kinase regulates several virulence detereminants. (2006)
  • Author(s): B. Venkatesh, Babujee, H. Liu, P. Hedley, T. Fujikawa, S. Tsuyumu
  • Journal Title: J. Bacteriol 188(8) Pages: 3088-3098
  • Peer Reviewed
• [Journal Article] Suppression of defense response in plants by the avrBs3/pthA gene family of Xanthomonas spp. Mol. (2006)
  • Author(s): T. Fujikawa, T. Yamashita, S. Tsuyumu
  • Journal Title: Plant-Microbe. Interact 19 Pages: 342-349
  • Peer Reviewed
• [Journal Article] Flagella-mediated motility is required for biofilm formation by Erwinia carotovora subsp. carotovora. (2006)
  • Author(s): M.M. Hossain, S. Tsuyumu
  • Journal Title: J. Gen. Plant Pathol 72 Pages: 34-39
  • NAID: 10017288023
  • Peer Reviewed
• [Journal Article] Bacterial Lipopolysaccharides Induce Defense Responses Associated with Programmed Cell Death in Rice Cells. (2006)
  • Author(s): Desaki, Miya, Venkatesh, Tsuyumu, Yamane, Kaku, Minami, Shibuya
  • Journal Title: Plant Cell Physiol. 47 Pages: 1530-1540
  • NAID: 10018652435
• [Journal Article] The Erwinia chrysanthemi 3937 PhoQ sensor kinase regulates several virulence detereminants. (2006)
  • Author(s): Venkatesh, Babujee, Liu, Hedley, Fujikawa, Birch, Toth, Tsuyumu
  • Journal Title: J. Bacteriol. 188(8) Pages: 3088-3098
• [Journal Article] Hypersensitive response suppression by type III effectors of plant pathogenic bacteria. (2006)
  • Author(s): T.Fujikawa, H.Ishihara, J.E.Leach, S.Tsuyumu
  • Journal Title: J. Gen. Plant Pathol. 72 Pages: 176-179
  • NAID: 10027220603
• [Journal Article] Suppression of defense response in plants by the avrBs3/pthA gene family of Xanthomonas spp. (2006)
  • Author(s): T.Fujikawa, T.Yamashita, S.Tsuyumu
  • Journal Title: Mol. Plant-Microbe. Interact. 19 Pages: 342-349
• [Journal Article] Suppression of defense response in plants by the avrBs3/pthA gene family of Xanthomonas spp (2006)
  • Author(s): T.Fujikawa, H.Ishihara, J.E.Leach, S.Tsuyumu
  • Journal Title: Mol.Plant-Microbe.Interact. 19 Pages: 342-349
• [Journal Article] HR suppression by type III effectors of plant pathogenic bacteria (2006)
  • Author(s): T.Fujikawa, T.Yamashita, S.Tsuyumu
  • Journal Title: J.Gen.Plant Pathol 72(3)(印刷中)
• [Journal Article] Virulence, resistance to magainin II, and expression of pectate lyase are controlled by the PhoP-PhoQ two-component regulatory system responding to pH and magnesium in Erwinia chrysanthemi 3937. (2005)
  • Author(s): Haque, M.M., S.Tsuyumu
  • Journal Title: J.Gen.Plant Pathol 71 Pages: 47-53
  • NAID: 10014472035
• [Journal Article] Virulence, accumulation of acetyl-coenzyme A and pectate lyase synthesis are controlled by PhoP-PhoQ two-component regulatory system responding to organic acids in Erwinia chrysanthemi 3937. (2005)
  • Author(s): Haque, M.M., A.Yamazaki, S.Tsuyumu
  • Journal Title: J.Gen.Plant Pathol. 71 Pages: 133-138
  • NAID: 10015507885
• [Journal Article] Motility is an important determinant for pathogenesis of Erwinia carotovora subsp.carotovora. (2005)
  • Author(s): Hossain, M.M., S.Shibata, S.Aizawa, S.Tsuyumu
  • Journal Title: Physiol.Mole.Plant Pathol. 66 Pages: 134-143
• [Presentation] A Role of Citrus Telomerase on Canker Formation Caused by Xanthomonas axonopodis pv. citri. (2009)
  • Author(s): Y.Ishiyama, T.Sekiya, A.Matsukura, H.Hirata, S.Tsuyumu
  • Organizer: Japan-Korea Joint Symposium
  • Place of Presentation: 韓国済州島
  • Year and Date: 2009-10-28
• [Presentation] Essential Region in Flagellin of Pectobacterium carotovrum subsp. carotovorum Responsible for Induction of Tobacco Cell Death (2009)
  • Author(s): H.Yamamoto, H.Hirata, S.Tsuyumu
  • Organizer: Japan-Korea Joint Symposium
  • Place of Presentation: 韓国済州島
  • Year and Date: 2009-10-28
• [Presentation] Studies on Molecular Plant-Microbe Interactions in Japan (2009)
  • Author(s): S.Tsuyumu, H.Hirata
  • Organizer: Japan-Korea Joint Symposium
  • Place of Presentation: 韓国済州島
  • Year and Date: 2009-10-08
• [Presentation] Pectobacterium carotovorum subsp. carotovorum由来フラジェリンにおける細胞死誘導必須部位の決定 (2009)
  • Author(s): 田中尊徳, ら
  • Organizer: 日本植物病理学会大会
  • Place of Presentation: 山形市
  • Year and Date: 2009-03-27
• [Presentation] Xanthomonas属細菌のエフェクター(AvrBs3/PthAファミリー)の抵抗性遺伝子Bs3プロモーター領域への結合解析 (2009)
  • Author(s): 奥出祥子, ら
  • Organizer: 日本植物病理学会大会
  • Place of Presentation: 山形市
  • Year and Date: 2009-03-27
• [Presentation] カンキツかいよう病菌の病徴発現に於けるテロメレースの役割 (2009)
  • Author(s): 石山佳幸, ら
  • Organizer: 日本植物病理学会大会
  • Place of Presentation: 山形市
  • Year and Date: 2009-03-27
• [Presentation] Pectobacterium carotovorum subsp. carotovorum 由来フラジェリンにおける細胞死誘導必須部位の決定 (2009)
  • Author(s): 田中尊徳, 井上望, 藤沢郁弥, 平田久笑, 露無慎二
  • Organizer: 日本植物病理学会大会
  • Place of Presentation: 山形大学
  • Year and Date: 2009-03-27
• [Presentation] Xanthomonas 属細菌のエフェクター (AvrBs3/PthAファミリー) の抵抗性遺伝子Bs3プロモーター領域への結合解析 (2009)
  • Author(s): 奥出祥子, 平田久笑, 露無慎二
  • Organizer: 日本植物病理学会大会
  • Place of Presentation: 山形大学
  • Year and Date: 2009-03-27
• [Presentation] カンキツかいよう病菌の病徴発現に於けるテロメレースの役割 (2009)
  • Author(s): 石山佳幸, 関谷敏芳, 松倉藍, 平田久笑, 露無慎二
  • Organizer: 日本植物病理学会大会
  • Place of Presentation: 山形大学
  • Year and Date: 2009-03-27
• [Presentation] Plant Phenolic compound o-coumaric acid enhances biofilm fomation and Pel Production in Dickeya dadantii 3937 (2009)
  • Author(s): J.S.Kabir, H.Hirata, S.Tsuyumu
  • Organizer: 日本植物病理学会大会
  • Place of Presentation: 山形大学
  • Year and Date: 2009-03-27
• [Presentation] 細菌感染がタバコモザイクウイルス (TMV) による局部病反形成に及ぼす影響 (2009)
  • Author(s): 石川典子, 平田久笑, 露無慎二
  • Organizer: 日本植物病理学会大会
  • Place of Presentation: 山形大学
  • Year and Date: 2009-03-27
• [Presentation] Pectobacterium 属細菌のエフェクター(AvrBs3/PthAファミリー)の抵抗性遺伝子Bs3プロモーター領域への結合 (2009)
  • Author(s): 奥出祥子, 平田久笑, 露無慎二
  • Organizer: 日本植物病理学会大会
  • Place of Presentation: 山形市
  • Year and Date: 2009-03-27
• [Presentation] Pectobacterium carotovorrm subsp. carotovorum由来フラジェリンにおける細胞致死誘導必須部位の決定 (2009)
  • Author(s): 田中尊徳, 井上望, 藤沢郁弥, 平田久笑, 露無慎二
  • Organizer: 日本植物病理学会大会
  • Place of Presentation: 山形市
  • Year and Date: 2009-03-27
• [Presentation] Plant phenoloic compound o-coumaric acid enhances biofilm formation and Pel production in Dickey a dadantii 3937. (2009)
  • Author(s): Kabir Md. S., Hirata H, Tsuvumu, S.
  • Organizer: 日本植物病理学会大会
  • Place of Presentation: 山形市
  • Year and Date: 2009-03-27
• [Presentation] 細菌感染がタバコモザイクウイルス(TMV)による局部病反形成に及ぼす影響(2) (2009)
  • Author(s): 石川典子, 平田久笑, 露無慎二
  • Organizer: 日本植物病理学会大会
  • Place of Presentation: 山形市
  • Year and Date: 2009-03-27
• [Presentation] カンキツかいよう病菌の病徴発現におけるテロメレースの役割 (2009)
  • Author(s): 石山佳幸, 関谷敏房, 松倉藍, 平田久笑, 露無慎二
  • Organizer: 日本植物病理学会大会
  • Place of Presentation: 山形市
  • Year and Date: 2009-03-27
• [Presentation] カンキツかいよう病菌によるかいよう形成におけるカンキツTERT(Telomerase reverse transcriptase)遺伝子の役割 (2008)
  • Author(s): 松倉藍, 関谷敏芳, 平田久笑, 露無慎二
  • Organizer: 日本植物病理学会
  • Place of Presentation: 松江市
  • Year and Date: 2008-04-26
• [Presentation] カンキツかいよう病菌検出のための発光遺伝子群luxAB融合ファージの作出 (2008)
  • Author(s): 武田佑輔, 長谷徳次, 平田久笑, 露無慎二
  • Organizer: 日本植物病理学会
  • Place of Presentation: 松江市
  • Year and Date: 2008-04-26
• [Presentation] Xanthomonas axonopodis pv. citriのかいよう形成因子Ap口の転写後修飾 (2008)
  • Author(s): 梅川沙希子, 川島沙由梨, 平田久笑, 露無慎二
  • Organizer: 日本植物病理学会
  • Place of Presentation: 松江市
  • Year and Date: 2008-04-26
• [Presentation] カンキツかいよう病菌に於けるペクチナーゼ制御因子KdgRに関する検鏡 (2008)
  • Author(s): 田中弘太, 山崎明広, 平田久笑, 露無慎二
  • Organizer: 日本植物病理学会
  • Place of Presentation: 松江市
  • Year and Date: 2008-04-26
• [Presentation] Pectobcterium carotovorum subsp. carotovorumのフラジェリンにおける細胞死誘導必須領域の決定 (2008)
  • Author(s): 井上望, 藤沢郁弥, 佐々木則英, 平田久笑, 露無慎二
  • Organizer: 日本植物病理学会
  • Place of Presentation: 松江市
  • Year and Date: 2008-04-26
• [Presentation] Significance of binding of Apll,canker forming factor,of Xanthomonas axonopodis pv.citri to Citrus pectin methyl esterase (2007)
  • Author(s): S. Kimura, K. Komai, H. Hirata, S. Tsuyumu
  • Organizer: 国際植物-微生物分子相互作用学会
  • Place of Presentation: イタリア、ソレント市
• [Book] 植物病理学(難波成任著) (2010)
  • Author(s): 真山滋志
  • Publisher: 文永堂出版
• [Remarks]
  • URL: http://www.agr.shizuoka.ac.jp/bs/plant_pathology/index.html
• [Remarks]
  • URL: http://www.shizuoka.ac.jp/index.html