Molecular Mechanisms of Vertebrate Photoperiodism

• Project/Area Number: 17108003
• Research Category: Grant-in-Aid for Scientific Research (S)
• Allocation Type: Single-year Grants
• Research Field: Basic veterinary science/Basic zootechnical science
• Research Institution: Nagoya University
• Principal Investigator: EBIHARA Shizufumi Nagoya University, 生命農学研究科, 教授 (50135331)
• Co-Investigator(Kenkyū-buntansha): MAEDA Keiichirou 名古屋大学, 大学院・生命農学研究科, 教授 (30181580) ; YOSHIMURA Takashi 名古屋大学, 大学院・生命農学研究科, 教授 (40291413) ; IIGO Masayuki 宇都宮大学, 農学部, 准教授 (10232109)
• Co-Investigator(Renkei-kenkyūsha): NAKAO Nobuhiro 名古屋大学, 大学院・生命農学研究科, 研究員 ; HW Korf Johann Wofgang Goethe-Universitat, 教授 ; YASUO Shinobu Johann Wofgang Goethe-Universitat, 研究員
• Project Period (FY): 2005 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥111,800,000 (Direct Cost: ¥86,000,000、Indirect Cost: ¥25,800,000); Fiscal Year 2009: ¥20,800,000 (Direct Cost: ¥16,000,000、Indirect Cost: ¥4,800,000); Fiscal Year 2008: ¥20,800,000 (Direct Cost: ¥16,000,000、Indirect Cost: ¥4,800,000); Fiscal Year 2007: ¥20,800,000 (Direct Cost: ¥16,000,000、Indirect Cost: ¥4,800,000); Fiscal Year 2006: ¥20,800,000 (Direct Cost: ¥16,000,000、Indirect Cost: ¥4,800,000); Fiscal Year 2005: ¥28,600,000 (Direct Cost: ¥22,000,000、Indirect Cost: ¥6,600,000)
• Keywords: 生理 / 行動 / 生産機能制御 / 光周性 / 甲状腺ホルモン / メラトニン / マウス / 気分障害 / 尾懸垂テスト / 脱ユビキチン化酵素 / 季節繁殖 / 脊椎動物 / 生殖腺 / 概日時計 / ウズラ

Research Abstract

The photoperiodic control of physiological phenomena (known as photoperiodism), such as flowering in plants and reproductive activity in animals is observed in a wide variety of species. However, the molecular mechanism of photoperiodism was not well understood in any organism studies. In the present study, we investigated a process from photic inputs to GnRH release in photoperiodism, focusing on the regulation and function of Dio2 gene using fish, birds and mammals.

Research Products

• [Journal Article] A mammalian neural tissue opsin(Opsin 5)is a deep brain photoreceptor in birds. (2010)
  • Author(s): Nakane Y, Ebihara S, Yoshimura T.
  • Journal Title: Proc Natl Acad Sci USA. 107(34) Pages: 15264-15268
  • Peer Reviewed
• [Journal Article] Usp46 is a quantitative trait gene regulating mouse immobile behavior in the tail suspension and forced swimming tests. (2009)
  • Author(s): Tomida S, Mamiya T, Abe K, Yoshimura T, Nabeshima T, Ebihara S.
  • Journal Title: Nat Genet. 41 Pages: 688-695
  • Peer Reviewed
• [Journal Article] Genetic and molecular analysis of wild-derived arrhythmic mice. (2009)
  • Author(s): Watanabe T, Yoshimura T, Ebihara S.
  • Journal Title: PLoS ONE. 4(1)
  • Peer Reviewed
• [Journal Article] Melatonin transmits photoperiodic signals through the MT1 melatonin receptor. (2009)
  • Author(s): Yasuo S, Yoshimura T, Ebihara S, Korf HW.
  • Journal Title: J.Neurosci. 29 Pages: 2885-2889
  • Peer Reviewed
• [Journal Article] Melatonin transmits photoperiodic signals through the MT1 melatonin receptor (2009)
  • Author(s): Yasuo S, et al.
  • Journal Title: J. Neurosci. 29 Pages: 2885-2889
  • Peer Reviewed
• [Journal Article] Genetic and molecular analys is of wild-derived arrhythmic mice (2009)
  • Author(s): Watanabe T, et al.
  • Journal Title: PLoS ONE 4
  • Peer Reviewed
• [Journal Article] Reorganization of the suprachiasmatic nucleus coding for day length. (2008)
  • Author(s): Naito E, Watanabe T, Tei H, Yoshimura T, Ebihara S.
  • Journal Title: J.Biol.Rhythms 23 Pages: 140-149
  • Peer Reviewed
• [Journal Article] Thyrotrophin in the pars tuberalis triggers photoperiodic response. (2008)
  • Author(s): Nakao N, Ebihara S, Ueda HR, Yoshimura T.
  • Journal Title: Nature 452 Pages: 317-322
  • Peer Reviewed
• [Journal Article] Involvement of thyrotropin in photoperiodic signal transduction in mice. (2008)
  • Author(s): Ono H, Ebihara S, Korf HW, Yoshimura T.
  • Journal Title: Proc Natl Acad Sci USA. 105(47) Pages: 18238-18242
  • Peer Reviewed
• [Journal Article] Involvement of thyrotropin in photoperiodic signal transduction in mice (2008)
  • Author(s): Ono H, et al.
  • Journal Title: Proo Natl Acad Sci USA. 105 Pages: 18238-18242
  • Peer Reviewed
• [Journal Article] Thyrotrophin in the pars tuberalis triggers photoperiodic response. (2008)
  • Author(s): N. Nakao, et. al.
  • Journal Title: Nature 452 Pages: 317-322
  • Peer Reviewed
• [Journal Article] Reorganiztion of the suprachiasmatic nucleus by photic and non-photic environments. (2008)
  • Author(s): S. Ebihara, et. al.
  • Journal Title: Sleep and Biological Rhythms (In press)
  • Peer Reviewed
• [Journal Article] Reorganization of the suprachiasmatic nucleus coding for day length. (2008)
  • Author(s): E. Naito, et. al.
  • Journal Title: J Biol Rhythms 23 Pages: 140-149
  • Peer Reviewed
• [Journal Article] Bimodal clock gene expression in mouse suprachiasmatic nucleus and peripheral tissues under a 7-hour light and 5-hour dark condition. (2007)
  • Author(s): T.Watanabe, T.Yoshimura, S.Ebihara.
  • Journal Title: J.Biol.Rhythms 22 Pages: 58-68
  • Peer Reviewed
• [Journal Article] Hypothalamic expression of thyroid hormone-activating and -inactivating enzyme genes in relation to photorefractoriness in birds and mammals. (2007)
  • Author(s): T.Watanabe, S.Ebihara, T.Yoshimura.
  • Journal Title: Am J Physiol Regul Integr Comp Physiol 292
  • Peer Reviewed
• [Journal Article] Differential response of type 2 deiodinase gene expression to photoperiod between photoperiodic Fischer 344 and nonphotoperiodic Wistar rats. (2007)
  • Author(s): S.Yasuo, S.Ebihara, T.Yoshimura.
  • Journal Title: Am J Physiol Regul Integr Comp Physiol. 292(3)
  • Peer Reviewed
• [Journal Article] Involvement of transforming growth factor α TGF in the photoperiodic regulation of reproduction in birds. (2007)
  • Author(s): T.Takagi, S.Ebihara, T.Yoshimura.
  • Journal Title: Endocrinology. 148(6) Pages: 2788-2792
  • Peer Reviewed
• [Journal Article] Circadian clock gene regulation of steroidogenic acute regulatory protein gene expression in preovulatory ovarian follicles. (2007)
  • Author(s): N.Nakao, S.Ebihara, T.Yoshimura.
  • Journal Title: Endocrinology 148 Pages: 3031-3038
  • Peer Reviewed
• [Journal Article] Photoperiodic changes in hypothalamic insulin receptor gene expression are regulated by gonadal testosterone. (2007)
  • Author(s): T.Anraku, S.Ebihara, T.Yoshimura.
  • Journal Title: Brain Res. 1163 Pages: 86-90
  • Peer Reviewed
• [Journal Article] Temporal dynamics of type 2 deiodinase expression after melatonin injections in Syrian hamsters. (2007)
  • Author(s): Yasuo S, Yoshimura T, Ebihara S, Korf HW.
  • Journal Title: Endocrinology 148 Pages: 4385-4392
  • Peer Reviewed
• [Journal Article] Molecular evolution of prepro-thyrotropin-releasing hormone in the chicken (Gallus gallus) and its expression in the brain. (2007)
  • Author(s): Y. Aoki, et. al.
  • Journal Title: Zoological Sci. 24 Pages: 686-692
  • Peer Reviewed
• [Journal Article] Bimodal clock gene expression in mouse suprachiasmatic nucleus and peripheral tissues under a 7-hour light and 5-hour dark condition. (2007)
  • Author(s): T.Watanabe et al.
  • Journal Title: J.Biol.Rhythms 22 Pages: 58-68
• [Journal Article] Hypothalamic expression of thyroid hormone-activating and -inactivating enzyme genes in relation to photorefractoriness in birds and mammals. (2007)
  • Author(s): T.Watanabe et al.
  • Journal Title: Am J Physiol Regul Integr Comp Physiol 292
• [Journal Article] Differential response of type 2 deiodinase gene expression to photoperiod between photoriodic fischer 344 and nonphotoperiodic Wistar rats. (2007)
  • Author(s): S.Yasuo et al.
  • Journal Title: Am J Physiol Regul Integr Comp Physiol 292
• [Journal Article] Long day suppressed expression of type 2 deiodinase gene in the mediobasal hypothalamus of the Saanen goat, a short day breeder : Implication for seasonal window of thyroid hormone action on reproductive neuroendocrine axis. (2006)
  • Author(s): S.Yasuo, S.Ebihara, T.Yoshimura.
  • Journal Title: Endocrinology 147 Pages: 432-440
  • Peer Reviewed
• [Journal Article] Peripheral clock gene expression in CS mice with bimodal locomotor rhythms. (2006)
  • Author(s): T.Watanabe, T.Yoshimura, S.Ebihara.
  • Journal Title: Neurosci Res. 54 Pages: 295-301
  • NAID: 10020617253
  • Peer Reviewed
• [Journal Article] Molecular mechanism of photoperiodic time measurement in the brain of Japanese quail. (2006)
  • Author(s): S.Yasuo, S.Ebihara, T.Yoshimura.
  • Journal Title: Chronobiology International 23 Pages: 307-315
  • Peer Reviewed
• [Journal Article] Possible involvement of organic anion transporting polypeptide 1c1 in the photoperiodic response of gonads in birds. (2006)
  • Author(s): N.Nakao, S.Ebihara, T.Yoshimura.
  • Journal Title: Endocrinology 147 Pages: 1067-1073
  • Peer Reviewed
• [Journal Article] T3 implantation mimics photoperiodically reduced encasement of nerve terminals by glial processes in the median eminence of Japanese quail. (2006)
  • Author(s): T.Yamamura, S.Yasuo, K.Hirunagi, S.Ebihara, T.Yoshimura.
  • Journal Title: Cell Tissue Res. 324 Pages: 175-179
  • Peer Reviewed
• [Journal Article] Long day suppressed expression of type-2 deiodinase gene in the mediobasal hypothalamus of the Saanen goat, a short day breeder : Implication for seasonal window of thyroid hormone action on reproductive neuroendocrine axis. (2006)
  • Author(s): S.Yasuo et al.
  • Journal Title: Endocrinology 147 Pages: 432-440
• [Journal Article] Peripheral clock gene expression in CS mice with bimodal locomotor rhythms. (2006)
  • Author(s): T.Watanabe et al.
  • Journal Title: Neurosci Res. 54 Pages: 295-301
• [Journal Article] Molecular mechanism of photoperiodic time measurement in the brain of Japanese quail. (2006)
  • Author(s): S.Yasuo et al.
  • Journal Title: Chronobiology International 23 Pages: 2307-2315
• [Journal Article] Molecular mechanism of the photoperiodic response of gonads in birds and mammals. (2006)
  • Author(s): T.Yoshimura et al.
  • Journal Title: Comparative Biochemistry and Physiology 144 Pages: 345-350
• [Journal Article] Possible involvement of organic anion transporting polypeptide lcl in the photoperiodic response of gonads in birds. (2006)
  • Author(s): N.Nakao et al.
  • Journal Title: Endocrinology 147 Pages: 1067-1073
• [Journal Article] T3 implantation mimics photoperiodically reduced encasement of nerve terminals by glial processes in the median eminence of Japanese quail. (2006)
  • Author(s): T.Yamamura et al.
  • Journal Title: Cell Tissue Res. 324 Pages: 175-179
• [Journal Article] T3 implantation mimics photoperiodically reduced encasement of nerve terminals by glial processes in the median eminence of Japanese quail. (2006)
  • Author(s): T.Yamamura et al.
  • Journal Title: Cell Tissue Res 324 Pages: 175-179
• [Journal Article] Possible involvement of organic anion transporting polypeptide 1cl in the photoperiodic response of gonads in birds. (2006)
  • Author(s): N.Nakao et al.
  • Journal Title: Endocrinology 147 Pages: 1067-1073
• [Journal Article] Peripheral clock gene expression in CS mice with bimodal locomotor rhythms. (2006)
  • Author(s): T.Watanabe et al.
  • Journal Title: Neurosci Res 54 Pages: 295-301
• [Journal Article] Long day suppressed expression of type 2 deiodinase gene in the mediobasal hypothalamus of the Saanen goat, a short day breeder : Implication for seasonal window of thyroid hormone action on reproductive neuroendocrine axis. (2006)
  • Author(s): S.Yasuo et al.
  • Journal Title: Endocrinology 147 Pages: 432-440
• [Journal Article] The reciprocal switching of two thyroid hormone-activating and -inactivating enzyme genes is involved in the photoperiodic gonadal response of Japanese quail. (2005)
  • Author(s): S.Yasuo, S.Ebihara, T.Yoshimura.
  • Journal Title: Endocrinology 146 Pages: 2551-2554
  • Peer Reviewed
• [Journal Article] Estrogen differentially regulates expressions of Per1 and Per2 genes between central and peripheral clocks, and between reproductive and non-reproductive tissues in female rats. (2005)
  • Author(s): T.J.Nakamura, S.Ebihara, K.Shinohara.
  • Journal Title: J Neurosci Res 82 Pages: 622-630
  • Peer Reviewed
• [Journal Article] The reciprocal switching of two thyroid hormone-activating and -inactivating enzyme genes is involved in the photoperiodic gonadal response of Japanese quail. (2005)
  • Author(s): S.Yasuo et al.
  • Journal Title: Endocrinology 146 Pages: 2551-2554
• [Journal Article] 生物はいかにして季節を読み取っているか?春を知らせる甲状腺ホルモン (2005)
  • Author(s): 山村崇 他
  • Journal Title: 化学と生物 43(3) Pages: 172-176
• [Journal Article] 脊椎動物の光周性 (2005)
  • Author(s): 中尾暢宏 他
  • Journal Title: 比較生理生化学 22(1) Pages: 20-26
  • NAID: 10025858028
• [Journal Article] 脊椎動物における光周性の制御機構 (2005)
  • Author(s): 海老原史樹文 他
  • Journal Title: 生物物理 45(4) Pages: 185-191
  • NAID: 10016628637
• [Journal Article] Involvement of TGF{alpha} in the photoperiodic regulation of reproduction in birds.
  • Author(s): T.Takagi et al.
  • Journal Title: Endocrinology (In press)
• [Journal Article] Molecular mechanism of the photoperiodic response of gonads in birds and mammals.
  • Author(s): T.Yoshimura
  • Journal Title: Comp Biochem Physiol (In press)
• [Journal Article] Molecular mechanism of photoperiodic time measurement in the brain of Japanese quail.
  • Author(s): S.Yasuo et al.
  • Journal Title: Chronobiol Intl (In press)
• [Presentation] マウスの「行動的絶望」を制御する遺伝子の特定 (2009)
  • Author(s): 海老原史樹文
  • Organizer: 理研筑波セミナー
  • Year and Date: 2009-05-12
• [Presentation] 行動的絶望"を司る量的形質遺伝子の特定-リズム異常を示すCSマウスを用いて- (2008)
  • Author(s): 海老原史樹文
  • Organizer: 日本遺伝学会第80回大会
  • Place of Presentation: 名古屋大学
  • Year and Date: 2008-09-03
• [Presentation] "行動的絶望"を司る遺伝子の特定 (2008)
  • Author(s): 海老原史樹文
  • Organizer: 川村浩先生アショフ・本間賞受賞記念シンポジウム
  • Place of Presentation: 三菱化学生命科学研究所
  • Year and Date: 2008-04-18
• [Presentation] "行動的絶望"を司る量的形質遺伝子の特定-リズム異常を示すCSマウスを用いて-、ワークショップ「生物時計研究の展開」 (2008)
  • Author(s): 海老原史樹文
  • Organizer: 日本遺伝学会第80回大会
• [Presentation] Reorganization of the suprachiasmatic nucleus by photic and non-photic environments. (2007)
  • Author(s): 海老原史樹文
  • Organizer: 2^<nd> Aschoff-Honma Prize Memorial Symposium.
  • Place of Presentation: Tokyo.
  • Year and Date: 2007-11-06
• [Presentation] MOLECULAR MECHANISMS OF PHOTOPERIODIC TIME MEASUREMENT IN BIRDS (2007)
  • Author(s): Yoshimura T.
  • Organizer: From Molecular clocks To Human Health Symposium
  • Place of Presentation: National Wine Centre of Australia
• [Presentation] 制限給餌による鳥類の概日リズム同調 (2007)
  • Author(s): 森健一郎, 他11名
  • Organizer: 第14回日本時間生物学会学術大会
  • Place of Presentation: 京王プラザホテル
• [Presentation] Molecular understanding of vertebrate photoperiodism. (2006)
  • Author(s): 海老原史樹文
  • Organizer: International Symposium of Chronomedicine and Biological Clocks.
  • Place of Presentation: Hokkaido University.
  • Year and Date: 2006-07-09
• [Presentation] Regional patterns of clock gene expression in the mouse SCN under different environmental conditions (2006)
  • Author(s): 海老原史樹文
  • Organizer: The 22nd International Symposium in Conjunction with Award of the International Prize for Biology "Chronobiology"
  • Place of Presentation: Tokyo International Forum
• [Presentation] (2006)
  • Author(s): 海老原史樹文
  • Organizer: 24th International Ornithological Congress
  • Place of Presentation: Hamburg, Germany
• [Presentation] Identification and characterization of thyroid hormone transporter mediating photoperiodic response in birds (2006)
  • Author(s): Nakao N.
  • Organizer: 10th Meeting of Society for Research on Biological Rhythms
• [Presentation] Fine tuning of photoperiodic time information in avian brain. (2005)
  • Author(s): S.Yasuo.
  • Organizer: 10^<th> Congress of the European Pineal and Biological Rhythms Society.
  • Place of Presentation: Frankfurt/Main, Germany
• [Book] 光周性の分子生物学 (2009)
  • Author(s): 海老原史樹文・井澤毅共編
  • Total Pages: 199
  • Publisher: シュプリンガー・ジャパン
• [Book] Melatonin regulation of clock genes in birds. In Functional Avian Endocrinology(A.Dawson & P.J.Sharp eds.) (2005)
  • Author(s): S.Ebihara, S.Yasuo, T.Yoshimura.
  • Publisher: Narosa, New Delhi
• [Book] Melatonin regulation of clock genes in birds. In Functional Avian Endocrinology(A.Dawson & P.J.Sharp eds.) (2005)
  • Author(s): S.Ebihara et al.
  • Total Pages: 447
  • Publisher: Narosa
• [Book] Melatonin regulation of clock genes in birds. In Functional Avian Endocrinology(A.Dawson & P.J.Sharp eds.) (2005)
  • Author(s): T.Yoshimura et al.
  • Total Pages: 447
  • Publisher: Narosa
• [Remarks] ホームページ
  • URL: http://www.agr.nagoya-u.ac.jp/~rhythm/
• [Patent(Industrial Property Rights)] 尾懸垂テスト及び強制水泳テストにおけるマウスの無動化を制御する遺伝子 (2008)
  • Inventor(s): 海老原史樹文, 冨田滋
  • Industrial Property Rights Holder: 国立大学法人名古屋大学
  • Filing Date: 2008-04-14