|Budget Amount *help
¥114,270,000 (Direct Cost: ¥87,900,000、Indirect Cost: ¥26,370,000)
Fiscal Year 2009: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2008: ¥27,690,000 (Direct Cost: ¥21,300,000、Indirect Cost: ¥6,390,000)
Fiscal Year 2007: ¥27,690,000 (Direct Cost: ¥21,300,000、Indirect Cost: ¥6,390,000)
Fiscal Year 2006: ¥22,100,000 (Direct Cost: ¥17,000,000、Indirect Cost: ¥5,100,000)
Fiscal Year 2005: ¥31,200,000 (Direct Cost: ¥24,000,000、Indirect Cost: ¥7,200,000)
(1)We elucidated the molecular mechanism for tethering p^<47> and p^<40> to cyt b558 or membrane on the structural basis.
(2)We found that IRF-3 is phosphorylated by IKKi at Ser386 and form a homodimer to bind CBP/p300in vitro and produces type Iinterferons.
(3)RIG-I like receptor family proteins (RLR) play essential roles to recognize double stranded RNA (dsRNA) in cytosol. We found that the C terminal domain of RLR recognizes viral specific dsRNA. Wedetermined the structure of C-terminal domain of RLR and discussed the specific interaction with viral specific dsRNA.
(4)dsRNA and LPS bind to TLR3 and TLR4, respectively to activate downstream signaling to producetype 1 interferons. Here, we determined the structure of TIR domains of TRIF and TRAM andelucidated their oligomerization process which is essential for down stream signaling.
(5)We elucidated the recognition mechanism of β1-3 glucan by βGRP from Bombyx Mori.