Elucidation of tolerance mechanism against peripheral target antigens in autoimmune diseases

• Project/Area Number: 17109012
• Research Category: Grant-in-Aid for Scientific Research (S)
• Allocation Type: Single-year Grants
• Research Field: Dermatology
• Research Institution: Keio University
• Principal Investigator: AMAGAI Masayuki 慶應大, 医学部, 教授 (90212563)
• Co-Investigator(Kenkyū-buntansha): KOYASU Shigeo 慶應義塾大学, 医学部, 教授 (90153684) ; KUWANA Masataka 慶應義塾大学, 医学部, 准教授 (50245479) ; ISHIKO Akira 慶應義塾大学, 医学部, 准教授 (10202988)
• Project Period (FY): 2005 – 2008
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥111,150,000 (Direct Cost: ¥85,500,000、Indirect Cost: ¥25,650,000); Fiscal Year 2009: ¥18,850,000 (Direct Cost: ¥14,500,000、Indirect Cost: ¥4,350,000); Fiscal Year 2008: ¥18,850,000 (Direct Cost: ¥14,500,000、Indirect Cost: ¥4,350,000); Fiscal Year 2007: ¥18,850,000 (Direct Cost: ¥14,500,000、Indirect Cost: ¥4,350,000); Fiscal Year 2006: ¥26,520,000 (Direct Cost: ¥20,400,000、Indirect Cost: ¥6,120,000); Fiscal Year 2005: ¥28,080,000 (Direct Cost: ¥21,600,000、Indirect Cost: ¥6,480,000)
• Keywords: 自己免疫 / 免疫寛容 / モデルマウス / 天疱瘡 / カドヘリン / 自己抗体 / 皮膚疾患 / 細胞接着

Research Abstract

The purpose of this study is to clarify the mechanisms of peripheral tolerance using pemphigus vulgaris which is mediated by IgG autoantibodies against desmoglein 3 (Dsg3) as a model disease. Dsg3-specific T cells showed pathogenic heterogeneity in anti-Dsg3 IgG production and IL-4 was a key cytokine in this process. A single helper T cell clone was sufficient to commit polyclonal naive B cells to produce pathogenic IgG. Mature Dsg3-specific B cells were eliminated via a Fas-mediated process in a CD4^+ T cell dependent fashion when tissue damages were induced by pathogenic IgG, suggesting a novel peripheral B cell tolerance mechanism.

Research Products

• [Journal Article] A single helper T cell clone is sufficient to commit polyclonal naive B cells to produce pathogenic IgG in experimental pemphigus vulgaris (2009)
  • Author(s): Takahashi H, Kuwana M, Amagai M
  • Journal Title: J Immunol 182(3) Pages: 1740-1745
• [Journal Article] Pemphigus mouse model as a tool to evaluate various immunosuppressive therapies. (2009)
  • Author(s): Takae Y, Nishikawa T, Amagai M
  • Journal Title: Exp Dermatol 18(3) Pages: 252-260
• [Journal Article] A single helper T cell clone is sufficient to commit polyclonal naive B cells to produce pathogenic IgG in experimental pemphigus vulgaris (2009)
  • Author(s): Takahashi H, Kuwana M, Amagai M
  • Journal Title: J Immunol 182 Pages: 1740-1745
  • Peer Reviewed
• [Journal Article] Genetic characterization of human Dsg3-specific B cells isolated by flow cytometry from the peripheral blood of patients with pemphigus vulgaris. (2008)
  • Author(s): Yamagami J, Takahashi H, Ota T, Amagai M
  • Journal Title: J Dermatol Sci 52(2) Pages: 98-107
  • NAID: 10024457486
• [Journal Article] Autoreactive B-cell elimination by pathogenic IgG specific for the same antigen: implications for peripheral tolerance. (2008)
  • Author(s): Ota T, Aoki-Ota M, Tsunoda K, Nishikawa T, Koyasu S, Amagai M
  • Journal Title: Int Immunol 20(10) Pages: 1351-1360
  • NAID: 10024357442
• [Journal Article] Novel system evaluating in vivo pathogenicity of desmoglein 3-reactive T cell clones using murine pemphigus vulgaris. (2008)
  • Author(s): Takahashi H, Amagai M, Nishikawa T, Fujii Y, Kawakami Y, Kuwana M
  • Journal Title: J Immunol 181(2) Pages: 1526-1535
• [Journal Article] Staphylococcal exfoliative toxins: "Molecular scissors" of bacteria that attack the cutaneous defense barrier in mammals. (2008)
  • Author(s): Nishifuji K, Sugai M, Amagai M
  • Journal Title: J Dermatol Sci 49(1) Pages: 21-31
  • NAID: 10024118117
• [Journal Article] Evaluating efficacy of plasmapheresis for patients with pemphigus using desmoglein enzyme-linked immunosorbent assay. (2008)
  • Author(s): Nagasaka T, Fujii Y, Ishida A, Handa M, Tanikawa A, Amagai M, Nishikawa T
  • Journal Title: Br J Dermatol 158(4) Pages: 685-690
• [Journal Article] Genetic characterization of human Dsg3-specific B cells isolated by flow cytometry from the peripheral blood of patients with pemphigus vulgaris. (2008)
  • Author(s): Yamagami J, Takahashi H, Ota T, Amagai M
  • Journal Title: J Dermatol Sci 52 Pages: 98-107
  • NAID: 10024457486
  • Peer Reviewed
• [Journal Article] Novel system evaluating in vivo pathogenicity of desmoglein 3-reactive T cell clones using murine pemphigus vulgaris (2008)
  • Author(s): Takahashi H, Amagai M, Nishikawa T, Fujii Y, Kawakami Y, Kuwana M
  • Journal Title: J Immunol 181 Pages: 1526-1535
  • Peer Reviewed
• [Journal Article] Pemphigus mouse model as a tool to evaluate various immunosuppressive therapies (2008)
  • Author(s): Takae Y, Nishikawa T, Amagai M
  • Journal Title: Exp Dermatol 18 Pages: 252-260
  • Peer Reviewed
• [Journal Article] Autoreactive B-cell elimination by pathogenic IgG specific for the same antigen : implications for peripheral tolerance (2008)
  • Author(s): Ota T, Aoki-Ota M, Tsunoda K, Nishikawa T, Koyasu S, Amagai M
  • Journal Title: Int Immunol 20 Pages: 1351-1360
  • NAID: 10024357442
  • Peer Reviewed
• [Journal Article] Evaluating efficacy of plasmapheresis for patients with pemphigus using desmoglein enzyme-linked immunosorbent assay (2008)
  • Author(s): Nagasaka T, et. al.
  • Journal Title: Br J Dermatol (In press)
  • Peer Reviewed
• [Journal Article] Development of tissue-targeting hemagglutinating virus of Japan envelope vector for successful delivery of therapeutic gene to mouse skin. (2007)
  • Author(s): Kawachi M, Tamai K, Saga K, Yamazaki T, Fujita H, Shimbo T, Kikuchi Y, Nimura K, Nishifuji K, Amagai M, Uitto J, Kaneda Y
  • Journal Title: Hum Gene Ther 18(10) Pages: 881-894
• [Journal Article] No activation of urokinase plasminogen activator by anti-desmoglein 3 monoclonal IgG antibodies in cultured human keratinocytes. (2007)
  • Author(s): Yamamoto Y, Aoyama Y, Shu E, Tsunoda K, Amagai M, Kitajima Y
  • Journal Title: J Dermatol Sci 47(2) Pages: 119-125
  • NAID: 10024116704
• [Journal Article] Anti-desmoglein 3 (Dsg3) monoclonal antibodies deplete desmosomes of Dsg3 and differ in their Dsg3-depleting activities related to pathogenicity. (2007)
  • Author(s): Yamamoto Y, Aoyama Y, Shu E, Tsunoda K, Amagai M, Kitajima Y
  • Journal Title: J Biol Chem 282(24) Pages: 17866-17876
• [Journal Article] T helper type 2-biased natural killer cell phenotype in patients with pemphigus vulgaris. (2007)
  • Author(s): Takahashi H, Amagai M, Tanikawa A, Suzuki S, Ikeda Y, Nishikawa T, Kawakami Y, Kuwana M
  • Journal Title: J Invest Dermatol 127 Pages: 324-330
• [Journal Article] Development of tissue-targeting hemagglutinating virus of Japan envelope vector for successful delivery of therapeutic gene to mouse skin (2007)
  • Author(s): Kawachi M, et. al.
  • Journal Title: Hum Gene Ther 18 Pages: 881-894
  • Peer Reviewed
• [Journal Article] Anti-desmoglein 3(Dsg3)monoclonal antibodies deplete desmosomes of Dsg3 and differ in their Dsg3-depleting activities related to pathogenicity (2007)
  • Author(s): Yamamoto Y, et. al.
  • Journal Title: J Biol Chem 282 Pages: 17866-17876
  • Peer Reviewed
• [Journal Article] Imaging and force spectroscopy on desmoglein 1 using atomic force microscopy reveal multivalent Ca(2+)-dependent,low-affinity trans-interaction (2007)
  • Author(s): Waschke J, et. al.
  • Journal Title: J Membr Biol 216 Pages: 83-92
  • Peer Reviewed
• [Journal Article] IgG autoantibodies directed against desmoglein 3 cause dissociation of keratinocytes in canine pemphigus vulgaris and paraneoplastic pemphigus (2007)
  • Author(s): Nishifuji K, et. al.
  • Journal Title: Vet Immunol Immunopathol 117 Pages: 209-221
  • Peer Reviewed
• [Journal Article] T helper type 2-biased natural killer cell phenotype in patients with pemphigus vulgaris (2007)
  • Author(s): Takahashi H, Amagai M, Tanikawa A, Suzuki S, Ikeda Y, Nishikawa T, Kawakami Y, Kuwana M
  • Journal Title: J Invest Dermatol 127・2 Pages: 324-330
• [Journal Article] Pemphigus, bullous impetigo, and the staphylococcal scalded-skin syndrome. (2006)
  • Author(s): Stanley J R, Amagai M
  • Journal Title: N Engl J Med 355 Pages: 1800-1810
• [Journal Article] Synergistic pathogenic effects of combined mouse monoclonal anti-desmoglein 3 IgG antibodies on pemphigus vulgaris blister formation. (2006)
  • Author(s): Kawasaki H, Tsunoda K, Hata T, Ishii K, Yamada T, Amagai M
  • Journal Title: J Invest Dermatol 126 Pages: 2621-2630
• [Journal Article] Tolerance induction by the blockade of CD40/CD154 interaction in pemphigus vulgaris mouse model. (2006)
  • Author(s): Aoki-Ota M, Kinoshita M, Ota T, Tsunoda K, Iwasaki T, Tanaka S, Koyasu S, Nishikawa T, Amagai M
  • Journal Title: J Invest Dermatol 126 Pages: 105-113
• [Journal Article] Enzyme-linked immunosorbent assay using bacterial recombinant proteins of human BP230 as a diagnostic tool for bullous pemphigoid. (2006)
  • Author(s): Yoshida M, Hamada T, Amagai M, Hashimoto K, Uehara R, Yamaguchi K, Imamura K, Okamoto E, Yasumoto S, Hashimoto T
  • Journal Title: J Dermatol Sci 41 Pages: 21-30
  • NAID: 10020550648
• [Journal Article] Desmoglein endocytosis and desmosome disassembly are coordinated responses to pemphigus autoantibodies. (2006)
  • Author(s): Calkins C C, Setzer S V, Jennings J M, Summers S, Tsunoda K, Amagai M, Kowalczyk A P
  • Journal Title: J Biol Chem 281 Pages: 7623-7634
• [Journal Article] Pathogenic monoclonal antibody against desmoglein 3 augments phosphorylation of desmoglein 3 and p38 MAP K in a human squamous carcinoma cell line. (2006)
  • Author(s): Kawasaki Y, Aoyama Y, Tsunoda K, Amagai M, Kitajima Y
  • Journal Title: Autoimmunity 39 Pages: 587-590
• [Journal Article] Production of Low Titers of Anti-Desmoglein 1 IgG Autoantibodies in Some Patients with Staphylococcal Scalded Skin Syndrome. (2006)
  • Author(s): Anzai H, Stanley J R, Amagai M
  • Journal Title: J Invest Dermatol 126 Pages: 2139-2141
• [Journal Article] Are desmoglein autoantibodies essential for the immunopathogenesis of pemphigus vulgaris, or just 'witnesses of disease'? (2006)
  • Author(s): Amagai M, Ahmed A R, Kitajima Y, Bystryn J C, Milner Y, Gniadecki R, Hertl M, Pincelli C, Fridkis-Hareli M, Aoyama Y, Frusic-Zlotkin M, Muller E, David M, Mimouni D, Vind-Kezunovic D, Michel B, Mahoney M, Grando S
  • Journal Title: Exp Dermatol 15 Pages: 815-831
• [Journal Article] Desmoglein endocytosis and desmosome disassembly are coordinated responses to pemphigus autoantibodies (2006)
  • Author(s): Calkins CC, Setzer SV, Jennings JM, Summers S, Tsunoda K, Amagai M, Kowalczyk AP
  • Journal Title: J Biol Chem 281・11 Pages: 7623-7634
• [Journal Article] Production of Low Titers of anti-Desmoglein 1 IgG Autoantibodies in Some Patients with Staphylococcal Scalded Skin Syndrome (2006)
  • Author(s): Anzai H, Stanley JR, Amagai M
  • Journal Title: J Invest Dermatol 126・9 Pages: 2139-2141
• [Journal Article] Are desmoglein atuoantibodies essential for the immunopathogenesis of pemphigus vulgaris, or just ′withnesses of disease′? (2006)
  • Author(s): Amagai M, Ahmed AR, Kitajima Y, Bystryn JC, Milner Y, et al.
  • Journal Title: Experimental Dermatology 15・10 Pages: 815-831
• [Journal Article] Pemphigus, bullous impetigo, and the staphylococcal scalded-skin syndrome (2006)
  • Author(s): Stanley JR, Amagai M
  • Journal Title: N Engi J Med 355・17 Pages: 1800-1810
• [Journal Article] Synergistic pathogenic effects of combined mouse monoclonal anti-desmoglein 3 IgG antibodies on pemphigus vulgaris blister formation (2006)
  • Author(s): Kawasaki H, Tsunoda K, Hata T, Ishii K, Yamada T, Amagai M
  • Journal Title: J Invest Dermatol 126・12 Pages: 2621-2630
• [Journal Article] Tolerance Induction by the Blockade of CD40/CD154 Interaction in Pemphigus Vulgaris Mouse Model. (2006)
  • Author(s): Aoki-Ota M, Kinoshita M, Ota T, Tsunoda K, Iwasaki T, Tanaka S, Koyasu S, Nishiawa T, Amagai M.
  • Journal Title: Invest Dermatol 126・1 Pages: 105-113
• [Journal Article] Genetic and functional characterization of human pemphigus vulgaris monoclonal autoantibodies isolated by phage display. (2005)
  • Author(s): Payne A S, Ishii K, Kacir S, Lin C, Li H, Hanakawa Y, Tsunoda K, Amagai M, Stanley J R, Siegel D L
  • Journal Title: J Clin Invest 115 Pages: 888-899
• [Journal Article] Cutaneous type pemphigus vulgaris: a rare clinical phenotype of pemphigus. (2005)
  • Author(s): Yoshida K, Takae Y, Saito H, Oka H, Tanikawa A, Amagai M, Nishikawa T
  • Journal Title: J Am Acad Dermatol 52 Pages: 839-845
• [Journal Article] n vitro keratinocyte dissociation assay for evaluation of the pathogenicity of anti-desmoglein 3 IgG autoantibodies in pemphigus vulgaris. (2005)
  • Author(s): Ishii K, Harada R, Matsuo I, Shirakata Y, Hashimoto K, Amagai M
  • Journal Title: J Invest Dermatol 124 Pages: 939-946
• [Journal Article] In vivo ultrastructural localization of the desmoglein 3 adhesive interface to the desmosome mid-line. (2005)
  • Author(s): Shimizu A, Ishiko A, Ota T, Saito H, Oka H, Tsunoda K, Amagai M, Nishikawa T
  • Journal Title: J Invest Dermatol 124 Pages: 984-989
• [Journal Article] In vitro keratinocyte dissociation assay for evaluation of the pathogenicity of anti-desmoglein 3 IgG autoantibodies in pemphigus vulgaris. (2005)
  • Author(s): Ishii K, Harada R, Matsuo I, Shirakata Y, Hashimoto K, Amagai M.
  • Journal Title: J Invest Dermatol 124・5 Pages: 939-946
• [Journal Article] In vivo ultrastructural localization of the desmoglein 3 adhesive interface to the desmosome mid-line. (2005)
  • Author(s): Shimizu A, Ishiko A, Ota T, Saito H, Oka H, Tsunoda K, Amagai M, Nishikawa T.
  • Journal Title: J Invest Dermatol 124・5 Pages: 984-989
• [Journal Article] Cutaneous type pemphigus vulgaris: a rare clinical phenotype of pemphigus. (2005)
  • Author(s): Yoshida K, Takae Y, Saito H, Oka H, Tanikawa A, Amagai M, Nishikawa T.
  • Journal Title: J Am Acad Dermatol 52・5 Pages: 839-845
• [Presentation] Characterization of T cells that regulate pathogenic IgG production in pemphigus model mice (2009)
  • Author(s): Amagai M
  • Organizer: 2009 Shanghai International Academic Meeting on Bullous Disease
  • Place of Presentation: Shanghai, China
  • Year and Date: 2009-03-21
• [Presentation] Cell adhesion molecules: targets of skin diseases (2008)
  • Author(s): Amagai M
  • Organizer: The 6th Wor ld Congr ess of Veter inary Dermatology
  • Place of Presentation: Hong Kong
• [Presentation] Pemphigus from molecules to mice to human (2008)
  • Author(s): Amagai M
  • Organizer: The 60th Annual Fall Meeting of Kor ean Dermatological Association
  • Place of Presentation: Seoul, Korea
• [Presentation] CD4+CD25+ regulatory T cells suppressing anti-Dsg3 antibody production in pemphigus vulgaris develop in the absence of Dsg3 (2008)
  • Author(s): Yokoyama T, Matsuda S, Takae Y, Amagai M, Koyasu S
  • Organizer: The International Investigative Dermatology 2008
  • Place of Presentation: Kyoto, Japan
• [Presentation] Pathogenic pemphigus foliaceus (PF) and vulgaris (PV) antibodies share a consensus amino acid motif in their CDR3 regions (2008)
  • Author(s): Yamagami J, Ishii K, Payne AS, Kacir S, Amagai M, Siegel DL, Stanley JR
  • Organizer: The International Investigative Dermatology 2008
  • Place of Presentation: Kyoto, Japan
• [Presentation] IgM to IgG isotype switching of anti-desmoglein 3 antibodies is an essential step for pathogenesis of pemphigus vulgaris (2008)
  • Author(s): Tsunoda K, Ota T, Hata T, Shimizu A, Yamada T, Nakagawa T, Amagai M
  • Organizer: The International Investigative Dermatology 2008
  • Place of Presentation: Kyoto, Japan
• [Presentation] Single desmoglein 3-reactive CD4+ T cell clones activate polyclonal naive B cells and promote the production of pathogenic IgG in experimental pemphigus vulgaris (2008)
  • Author(s): Takahashi H, Kuwana M, Amagai M
  • Organizer: The International Investigative Dermatology 2008
  • Place of Presentation: Kyoto, Japan
• [Presentation] Two pathogenic anti-desmoglein 1 (Dsg1) monoclonal antibodies isolated from one pemphigus foliaceus (PF) patient define major Dsg1 epitopes recognized by most pemphigus patients (2008)
  • Author(s): Ishii K, Adly M, Yokouchi M, Stanley JR, Amagai M
  • Organizer: The International Investigative Dermatology 2008
  • Place of Presentation: Kyoto, Japan
• [Presentation] Induction of both humoral and cellular immune response against desmoglein 3 provides a mouse model mimicking an immunological aspect of paraneoplastic pemphigus (2008)
  • Author(s): Hata T, Nishifuji K, Yamada T, Shimoda K, Amagai M
  • Organizer: The International Investigative Dermatology 2008
  • Place of Presentation: Kyoto, Japan
• [Presentation] Proteolytic processing of precursor desmoglein 1 (Dsg1) unmarks pathogenic epitope (2008)
  • Author(s): Yokouchi M, Ishii K, Stanley JR, Amagai M
  • Organizer: Post IID 2008 Satellite: International Meeting on Autoimmune Bullous Diseases Otsu
  • Place of Presentation: Japan
• [Presentation] Mechanisms of desmosome disassembly in pemphigus vulgaris (2008)
  • Author(s): Kowalczyk A, Kottke M, Jennings JM, Delva E, Hanakawa Y, Amagai M
  • Organizer: Post IID 2008 Satellite: International Meeting on Autoimmune Bullous Diseases Otsu
  • Place of Presentation: Japan
• [Presentation] Mechanisms of desmosome disassembly in pemphigus vulgaris (2008)
  • Author(s): Kowalczyk A, Kottke M, Jennings JM, Delva E, Hanakawa Y, Amagai M.
  • Organizer: Post IID 2008 Satellite : International Meeting on Autoimmune Bullous Diseases
  • Place of Presentation: Otsu, Japan
• [Presentation] IgM to IgG isotype switching of anti-desmoglein 3 antibodies is anessential step for pathogenesis of pemphigus vulgaris (2008)
  • Author(s): Tsunoda K, Ota T, Hata T, Shimizu A, Yamada T, Nakagawa T, Amagai M
  • Organizer: The International Investigative Dermatology2008
  • Place of Presentation: Kyoto, Japan
• [Presentation] Two pathogenic anti-desmoglein 1 (Dsg1) monoclonal antibodies isolated from one pemphigus foliaceus (PF)patient define major Dsg1 epitopes recognized by most pemphigus patients (2008)
  • Author(s): Ishii K, Adly M, Yokouochi M, Stanley JR, Amagai M
  • Organizer: The International Investigative Dermatology 2008
  • Place of Presentation: Kyoto, Japan
• [Presentation] Single desmoglein 3-reactive CD4+ T cell clones activate polyolonalnaive B cells and promote the production of pathogenic IgG inexperimental pemphigus vulgaris (2008)
  • Author(s): Takahashi H, Kuwana M, Amagai M
  • Organizer: The International Investigative Dermatology 2008
  • Place of Presentation: Kyoto, Japan
• [Presentation] Pemphigus from molecules to mice to human (2008)
  • Author(s): Amagai M
  • Organizer: The 60th Annual Fall Meeting of Korean Dermatological Association
  • Place of Presentation: Seoul, Korea
• [Presentation] Pemphigus with a simple logic behind complex phenotype (2007)
  • Author(s): Amagai M
  • Organizer: Hong Kong Society of Dermatology and Venereology Annual Scieentific Meeting 2007
  • Place of Presentation: Hong Kong,China
  • Year and Date: 2007-06-24
• [Presentation] Development of targeted therapies for autoimmune bullous diseases (2007)
  • Author(s): Amagai M
  • Organizer: 21st World Congr ess of Dermatology
  • Place of Presentation: Buenos Aires, Argentina
• [Presentation] Animal models- Do they tell us about human disease? (2007)
  • Author(s): Amagai M
  • Organizer: 21st Wor ld Congr ess of Dermatology
  • Place of Presentation: Buenos Aires, Argentina
• [Presentation] Suppressive effects of CD4+ CD25+ regulatory T cells on the development of phenotypes in a mouse model of pemphigus vulgaris (2007)
  • Author(s): Yokoyama T, Matsuda S, Takae Y, Amagai M, Koyasu S
  • Organizer: The 37th Annual Meeting of European Society for Dermatological Research (ESDR)
  • Place of Presentation: Zurich, Switzland
• [Presentation] Genetic characterlization of human Dsg3-specific B cells isolated by flow cytmetry from peripheral blood of patients with pemphigus vulgaris (2007)
  • Author(s): Yamagami J, Takahashi H, Ota T, Amagai M
  • Organizer: The 37th Annual Meeting of European Society for Dermatological Resear ch (ESDR)
  • Place of Presentation: Zurich, Switzland
• [Presentation] Effective B cell depletion therapy in a pemphigus mouse model (2007)
  • Author(s): Takae Y, Hamaguchi Y, Tedder TF, Amagai M
  • Organizer: The 68th Annual Meeting of the Society for Investigative Dermatology
  • Place of Presentation: Los Angeles, USA
• [Presentation] Effective B cell depletion therapy in a pemphigus mouse model (2007)
  • Author(s): Takae Y, et. al.
  • Organizer: The 68th Annual Meeting of the Society for Investigative Dermatology
  • Place of Presentation: Los Angeles,USA
• [Presentation] Evaluation of various immunosuppressive therapies using pemphigus mouse model (2007)
  • Author(s): Takae Y, et. al.
  • Organizer: 13th International Congress of Mucosal Immunology
  • Place of Presentation: Tokyo,Japan
• [Presentation] Genetic characterlization of human Dsg3-specific B cells isolated by flow cytmetryfrom periphelal blood of patients with pemphigus vulgahs (2007)
  • Author(s): Yamagami J, et. al.
  • Organizer: The 37th Annual Meeting of European Society for Dermatological Research(ESDR)
  • Place of Presentation: Zurich,Switzland
• [Presentation] Suppressive effects of CD4+ CD25+ regulatoly T cells on the develo pment of phenotypes in a mouse model of pemphigus vulgaris (2007)
  • Author(s): Yokoyama T, et. al.
  • Organizer: The 37th Annual Meeting of European Society for Dermatological Research(ESDR)
  • Place of Presentation: Zurich,Switzland
• [Presentation] Cadherins as autoimmune targets in pemphigu (2007)
  • Author(s): Amagai M
  • Organizer: International Symposium of the SFB 487: Membrane Proteins & Diseases
  • Place of Presentation: Wuerzburg,Germany
• [Presentation] Animal models-Do they tell us about human disease? (2007)
  • Author(s): Amagai M
  • Organizer: 21st World Congress of Dermatology
  • Place of Presentation: Buenos Aires,Argentina
• [Presentation] Development of targeted therapies for autoimmune bullous diseases (2007)
  • Author(s): Amagai M
  • Organizer: 21st World Congress of Dermatology
  • Place of Presentation: Buenos Aires,Argentina
• [Presentation] Genetic restriction of anti-desmoglein(Dsg)1 antibodies in pemphigus foliaceus (PF) (2006)
  • Author(s): Ishii K, Li H, Lin C, Kacir S, Amagai M, Siegel DL, Stanley JR
  • Organizer: The 67th Annual Meeting of the Society for Investigative Dermatology
  • Place of Presentation: Philadelphia, PA, USA
• [Presentation] Characterization of desmoglein 3-reactive helper T cells involved in pathogenic IgG production in a mouse model for pemphigus vulgaris (2006)
  • Author(s): Takahashi H, Amagai M, Nishikawa T, Kawakami Y, Kuwana M
  • Organizer: The 67th Annual Meeting of the Society for Investigative Dermatology
  • Place of Presentation: Philadelphia, PA, USA
• [Presentation] Binding kinetic analysis of anti-desmoglein 3 monoclonal antibodies by surface plasmon resonance technology (2006)
  • Author(s): Tsunoda K, Iemura S, Nakagawa T, Natsume T, Amagai M
  • Organizer: The 67th Annual Meeting of the Society for Investigative Dermatology
  • Place of Presentation: Philadelphia, PA, USA
• [Presentation] Dsg1 transgenic rescue of loss of cell adhesion in Dsg3 deficient mice (2005)
  • Author(s): Hata T, Nishifuji K, Shimoda K, Yamada T, Nishikawa T, Amagai M
  • Organizer: The 66th Annual Meeting of the Society for Investigative Dermatology
  • Place of Presentation: St. Louis, Missouri, USA
• [Presentation] Danger signal by pathogenic IgG eliminates autoreactive B cells against desmoglein 3 (2005)
  • Author(s): Ota T, Aoki-Ota M, Tsunoda K, Nishikawa T, Koyasu S, Amagai M
  • Organizer: desmoglein 3. The 66th Annual Meeting of the Society for Investigative Dermatology
  • Place of Presentation: St. Louis, Missouri, USA
• [Presentation] Expression of desmoglein 3 in medullary thymic epithelial cells in thymus (2005)
  • Author(s): Wada N, Nishifuji K, Yamada T, Kudoh J, Shimizu N, Peltonen L, Nagafuchi S, Amagai M
  • Organizer: The 66th Annual Meeting of the Society for Investigative Dermatology
  • Place of Presentation: St. Louis, Missouri, USA
• [Book] Clinical and Basic Immunodermatology (2009)
  • Author(s): Amagai M
  • Publisher: Springer London Ltd
• [Book] Pemphigus. Dermatology 2nd Edition (2008)
  • Author(s): Amagai M
  • Publisher: Mosby Elsevier
• [Book] Dermatologic Immunity (2008)
  • Author(s): Amagai M
  • Publisher: Karger, Basel
• [Book] Dermatology 2nd Edition (2008)
  • Author(s): Amagai M
  • Publisher: Mosby Elsevier
• [Remarks]
  • URL: http://web.sc.itc.keio.ac.jp/derma/
• [Remarks]
  • URL: http://web.sc.itc.keio.ac.jp/derma/index.html