Comprehensive identification and analysis of extracellular signaling molecules involved in vascular tissue formation
| Project/Area Number |
17207004
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
植物生理・分子
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| Research Institution | The University of Tokyo |
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Principal Investigator |
FUKUDA Hiroo The University of Tokyo, Graduate School of Science, Department of Sciences, Professor (10165293)
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| Co-Investigator(Kenkyū-buntansha) |
SAWA Shimichiro University of Tokyo, Graduate School of Science, Department of Sciences, Professor (00315748)
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| Project Period (FY) |
2005 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥51,610,000 (Direct Cost: ¥39,700,000、Indirect Cost: ¥11,910,000)
Fiscal Year 2007: ¥14,040,000 (Direct Cost: ¥10,800,000、Indirect Cost: ¥3,240,000)
Fiscal Year 2006: ¥13,000,000 (Direct Cost: ¥10,000,000、Indirect Cost: ¥3,000,000)
Fiscal Year 2005: ¥24,570,000 (Direct Cost: ¥18,900,000、Indirect Cost: ¥5,670,000)
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| Keywords | Zinnea elegance / CLE / stem cell / tracheary element / meristem / peptide / folic acid / Arabidopsis thaliana / ブラシノステロイド / 前形成層 / 管状要素 / TOF-MS / プログラム細胞死 / ヌクレアーゼ / リパーゼ / システインプロテアーゼ / マイクロアレイ |
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| Research Abstract |
1. Isolation and characterization of TDIF: We succeeded in isolating TDIF, which is composed of 12 amino acid residues with two hydroxyproline residues and suppresses tracheary element differentiation. The gene for TDIF belonged to the CLE gene family. 2. Identification of the CLV3 peptide: We examined the activity of various size of CLV3 peptides against the shoot meristem formation, and found that 12 amino acids with 2 hydroxyproline residues are necessary and sufficient to suppress the meristem formation. 3. Various functions of CLE peptides: Twenty six chemically synthesized CLE peptides were tested for activities for various events including tracheary element differentiation and found their specificity and redundancy. 4. Analysis of xylogen homologues: We found 15 genes related to the xylogen genes in Arabidopsis genome and examined their expression pattern. Some of them were expressed preferentially in vascular tissues, but the others were expressed in distinctive tissues, suggesting their distinctive functions. 5. Analysis of procambium-specific genes using a gene trap line: We succeeded in the isolation of several genes that are expressed in preferentially in the procambium. 6. Analysis of the gamma-glutamyl hydrolase (GGH) gene: GGH1 is one of the genes that are expressed preferentially in the procambium. GGH shortens polyglutamate chains of the poly glutamate type of folic acid. The loss-of-function and gain-of-function analyses of GGHI, GGH2 and GGH3 indicated that these genes are involved in meristem formation. Taken together with results from in vitro experiments, we proposed that the poly glutamate type of folic acid plays a critical role in maintenance of stem cells in meristem.
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Report
(4 results)
Research Products
(146 results)
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[Journal Article] Transient transformation and RNA silencing in Zinnia tracheary element differentiating cell cultures2008
Author(s)
Endo, S., Pesquet, E, Tashiro, G., Kuriyama, H., Goffner, D., Fukuda, H.and Demura, T.
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Peer Reviewed
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[Journal Article] Comprehensive analysis of glucan elicitor-regulated gene expresslon intobacco BY-2 Cells reveals a novel MYB transcription factor in volved in the regulation of phenylpropanoid metabolism2007
Author(s)
Shinya, T., Galis, I., Narisawa, T., Sasaki, M., Fukuda.H., Matsuoka, E., Saito, M. and Matsuoka, K.
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Journal Title
Plant Cell Physiol 48
Pages: 1404-1413
Related Report
Peer Reviewed
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[Journal Article] Gain-of-function phenotypes of chemically synthetic CLAVATA3/ESR-related(CLE)pepdes in Arabidopsis thaliana and Oryza sativa2007
Author(s)
Kinoshita, A., Nakamura, Y, , Sasaki, E, Kyozuka, J., Fukuda H.and Sawa, S.
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Journal Title
Plant Cell Physiol 48
Pages: 1821-1825
Related Report
Peer Reviewed
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[Journal Article] Co-regulation of brassinosteroid biosynthesis-related genes during xylem cell differentiation.2007
Author(s)
Yamamoto, R., Fujioka, S., Iwamoto, K., Demura, T., Takatsuto, S. Yoshida, S. and Fukuda, H.
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Journal Title
Plant Cell Physiol. 48
Pages: 74-83
NAID
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[Journal Article] Gene expression profiling using cDNA microarray analysis of the sexual reproduction stage of the unicellular charophycean alga Closterium peracerosum-strigosum-littorale complex.2006
Author(s)
Sekimoto, H., Tanabe, Y., Tsuchikane, Y., Shirosaki, H., Fukuda, H., Demura, T., Ito, M.
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Journal Title
Plant Physiol. 141
Pages: 271-279
Related Report
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[Journal Article] A novel R2R3 MYB transcription factor NtMYBJS1 is a methyl jasmonate-dependent regulator of phenylpropanoid-conjugate biosynthesis in tobacco.2006
Author(s)
Galis, I., Simek, P., Narisawa, T., Sasaki, M., Horiguchi, T., Fukuda, H. and Matsuoka, K.
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Journal Title
Plant J. 46
Pages: 573-592
Related Report
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[Journal Article] Fluorescence cross-correlation analyses of molecular interaction between an Aux/IAA protein, MSG2/IAA19, and protein-protein interaction domains of auxin response factors of Arabidopsis expressed in HeLa cells.2006
Author(s)
Muto, H., Nagao, I., Demura, T., Fukuda, H., Kinjo, M., and Yamamoto, K.
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Journal Title
Plant Cell Physiol. 47
Pages: 1095-1101
NAID
Related Report
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[Journal Article] Dodeca-CLE peptides as suppressors of plant stem cell.2006
Author(s)
Ito, Y., Nakanomyo, I., Motose, H., Iwamoto, K., Sawa, S., Dohmae, N., and Fukuda, H.
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Journal Title
Science 313
Pages: 842-845
Related Report
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[Journal Article] A plant peptide encoded by CLV3 identified by in situ MALDITOF-MS analysis.2006
Author(s)
Kondo, T., Sawa, S., Kinoshita, A., Mizuno, S., Kakimoto, T., Fukuda, H., and Sakagami, Y.
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Journal Title
Science 313
Pages: 845-848
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[Journal Article] Gene trapping in Arabidopsis reveals genes involved in vascular development.2006
Author(s)
Nagawa, S., Sawa, S., Sato, S., Kato, T., Tabata, S., and Fukuda, H.
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Journal Title
Plant Cell Physiol. 47
Pages: 1394-1405
NAID
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[Presentation] Function of dodeca-CLE peptides in plant stem cells(invited)2007
Author(s)
Fukuda, H., Nakanomyo, I., Inoue, A., Kinoshita, A., Sawa, S.
Organizer
IPGSA
Place of Presentation
Puerto Vallarta,Mexico
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