Evolution of microtubule organizing system in land plants in terms of dynamics and nano-structures of microtubule ends

• Project/Area Number: 17207006
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Morphology/Structure
• Research Institution: University of Hyogo
• Principal Investigator: MINEYUKI Yoshinobu University of Hyogo, 大学院・生命理学研究科, 教授 (30219703)
• Co-Investigator(Kenkyū-buntansha): 唐原 一郎 富山大学, 大学院・理工学研究科, 准教授 (60283058) ; 中井 朋則 兵庫県立大学, 大学院・生命理学研究科, 助教 (60347531)
• Co-Investigator(Renkei-kenkyūsha): STAEHELIN L. Andrew コロラド大学, MCD Biology, 教授
• Project Period (FY): 2005 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥51,480,000 (Direct Cost: ¥39,600,000、Indirect Cost: ¥11,880,000); Fiscal Year 2008: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000); Fiscal Year 2007: ¥11,830,000 (Direct Cost: ¥9,100,000、Indirect Cost: ¥2,730,000); Fiscal Year 2006: ¥12,610,000 (Direct Cost: ¥9,700,000、Indirect Cost: ¥2,910,000); Fiscal Year 2005: ¥23,790,000 (Direct Cost: ¥18,300,000、Indirect Cost: ¥5,490,000)
• Keywords: 植物 / ナノマシン / 細胞・組織 / 生体分子 / ガンマチューブリン / 微小管形成中心 / 電子線トモグラフィー / 陸上植物

Research Abstract

動物は微小管形成の制御装置として中心体を持つが、植物は中心体なしで微小管形成を行っている。本研究では、微小管の重合開始に必要なγチューブリン分子とγチューブリンが作る微小管端のキャップ構造に注目して、植物特有の微小管形成システムは何かを調べた。その結果、被子植物ではキャップ構造が不安定なことが示唆できた。また、コケ・シダ・裸子植物で保存され、被子植物で変化するγチューブリン分子表面に存在する十数個のアミノ酸が見つかった。キャップ構造の安定性とγチューブリン構造の変化との関係は今後の課題となった。

Research Products

• [Journal Article] The preprophase band is a localized center of clathrin-mediated endocytosis in late prophase cells of the onion cotyledon epidermis (2009)
  • Author(s): Karahara, I., Suda, J., Tahara, H., Yokota, E., Shimmen, T., Misaki, K., Yonemura, S., Staehelin, L.A., and Mineyuki, Y
  • Journal Title: Plant J 57 Pages: 813-831
  • Peer Reviewed
• [Journal Article] The preprophase band is a localized center of clathrin-mediated endocytosis in late prophase cells of the onion cotyledon epidermis (2009)
  • Author(s): Karahara, I.
  • Journal Title: Plant Journal 57 Pages: 819-831
  • Peer Reviewed
• [Journal Article] Plant microtubule studies : past and present (2007)
  • Author(s): Mineyuki, Y
  • Journal Title: J. Plant Res 120 Pages: 45-51
  • NAID: 10018828444
  • Peer Reviewed
• [Journal Article] γ-Tubulin-like molecules in the mouse duodenal epithelium (2007)
  • Author(s): Suzaki, E., Nomura, R., Horio, T., Mineyuki, Y., and Kataoka, K
  • Journal Title: Histochem. Cell Biol 128 Pages: 175-182
  • Peer Reviewed
• [Journal Article] 電子線トモグラフィーとは何か : ナノスケールでの3Dバイオイメージング (2007)
  • Author(s): 唐原一郎, 須田甚将, 峰雪芳宣
  • Journal Title: 細胞工学 26 Pages: 696-701
• [Journal Article] γ-Tubuhlin-like molecules in the mous eduodenal epithelium. (2007)
  • Author(s): Suzaki, Etsuko
  • Journal Title: Histochem.Cell Biol 128 Pages: 175-182
  • Peer Reviewed
• [Journal Article] 電子線トモグラフィーとは何か:ナノスケールでの3Dバイオイメージング (2007)
  • Author(s): 唐原 一郎
  • Journal Title: 細胞工学 26 Pages: 696-701
• [Journal Article] Plant microtubule studies : past and present (2007)
  • Author(s): Yoshinobu Mineyuki
  • Journal Title: Journal of Plant Research 120 Pages: 45-51
  • NAID: 10018828444
• [Journal Article] Membrane?associated microtubuleorganizing centers in basal land plants (2005)
  • Author(s): Mineyuki, Y., and Shimamura, M
  • Journal Title: Cytologia 70
• [Journal Article] Membrane-associated microtubule-organizing centers in basal land plants (2005)
  • Author(s): Yoshinobu Mineyuki, Masaki Shimamura
  • Journal Title: Cytologia 70・3
• [Journal Article] Quantitative analysis of vesicles in the preprophase band by electron tomography
  • Author(s): Karahara, I., Suda, J., Staehelin, L.A., and Mineyuki, Y
  • Journal Title: Cytologia (in press)
  • NAID: 10025405365
• [Presentation] Changes in function and amino acid residues of γ-tubulin in the evolution of green plants (2009)
  • Author(s): Miyamoto, Y., Horio, T., Yamauchi, D., Okada, T., Sekita, S., Futamura, N., Shinohara, K., Fujimoto, Y., Nakai, T., Shimmen, T., Shimamura, M., Akashi, T., and Mineyuki, Y
  • Organizer: 第61回日本細胞生物学会大会
  • Place of Presentation: 名古屋市
• [Presentation] Electron tomographic analysis of the plant cell cortex : The preprophase band is a localized center of clathrin-mediated endocytosis (2009)
  • Author(s): Karahara, I., Suda, J., Tahara, H., Yokota, E., Shimmen, T., Misaki, K., Yonemura, S., Staehelin, L., and Mineyuki, Y
  • Organizer: Asia-Pacific Congress on Electron Tomography
  • Place of Presentation: Brisbane, Australia
• [Presentation] Electron tomographic analysis of microtubulemicrofilament interactions in the epidermal cell cortex of high pressure frozen cotyledons (2009)
  • Author(s): Takeuchi, M., Karahara, I., Kajimura, N., Takaoka, A., Misaki, K., Yonemura, S., Staehelin, L., and Mineyuki, Y
  • Organizer: Asia-Pacific Congress on Electron Tomography
  • Place of Presentation: Brisbane, Australia
• [Presentation] Electron tomographic analysis of the plant cell cortex : The preprophase band is a localized center of clathrin-mediated endocytosis. (2009)
  • Author(s): Karahara, I.
  • Organizer: Asia-Pacific Congress on Electron Tomography
  • Place of Presentation: クイーンズランド大学(ブリスベン, オーストラリア)
• [Presentation] Preprophase band is a localized center of clathrin-mediated endocytosis in late prophase of onion cotyledon epidermis (2008)
  • Author(s): Karahara, I., Suda, J., Masuta, Y., Tahara, H., Yokota, E., Shimmen, T., Misaki, K., Yonemura, S., Staehelin, L., and Mineyuki, Y
  • Organizer: 9th Asia-Pacific Microscopy Conference
  • Place of Presentation: Jeju, Republic of Korea
• [Presentation] Plant γ-tubulins have gradually lost the function that is essential for unikonts cells during their evolution (2008)
  • Author(s): Miyamoto, Y., Yamauchi, D., Kuno, R., Nakai, T., Futamura, N., Shinohara, K., Shimmen, T., Shimamura, M., Akashi, T., Horio, T., and Mineyuki, Y
  • Organizer: 2nd International Symposium on Bio-nanosystems
  • Place of Presentation: Tokyo
• [Presentation] 植物細胞表層の電子線トモグラフィー解析 (2008)
  • Author(s): 唐原一郎, 峰雪芳宣
  • Organizer: 日本植物学会第72回大会 シンポジウム
  • Place of Presentation: 高知
• [Presentation] 加圧凍結・2軸電子線トモグラフィー法による微小管構築機構の解析 (2008)
  • Author(s): 峰雪芳宣, 唐原一郎
  • Organizer: 第49回日本植物生理学会年会シンポジウム"植物細胞の内骨格と外骨格"
  • Place of Presentation: 札幌
• [Presentation] Analysis of Membrane/Cytoskeletal Systems in Plant Cell Cortex (2008)
  • Author(s): Mineyuki, Y
  • Organizer: International Symposium on Picobiology
  • Place of Presentation: Kamigori, Hyogo
• [Presentation] 陸上植物γ-チューブリンのアミノ酸配列変化と機能変遷 (2008)
  • Author(s): 宮本裕子
  • Organizer: 日本植物学会大72回大会
  • Place of Presentation: 高知大学(高知市)
• [Presentation] Preprophase band is a localized center of clathrin-mediated endocytosis in late prophase of onion cotyledon epidermis (2008)
  • Author(s): Karahara, I.
  • Organizer: 9^<th> Asia-Pacific Microscopy Conference
  • Place of Presentation: Jeju International Convention Center (済州島、韓国)
• [Presentation] Analysis of Membrane/Cytoskeletal Systems in Plant Cell Cortex (2008)
  • Author(s): Mineyuki, Yoshinobu
  • Organizer: International Symposium on Picobiology
  • Place of Presentation: 兵庫県上郡町
• [Presentation] 加圧凍結・2軸電子線トモグラフィー法による微小管構築機構の解析 (2008)
  • Author(s): 峰雪 芳宣
  • Organizer: 第49回日本植物生理学会年会
  • Place of Presentation: 札幌市
• [Presentation] Electron tomographic analysis of preprophase band (2007)
  • Author(s): Mineyuki, Yoshinobu
  • Organizer: Andrew Staehelin's Retirement Symposium
  • Place of Presentation: ボルダー市コロラド大学
  • Year and Date: 2007-06-16
• [Presentation] Electron tomographic analysis of preprophase band (2007)
  • Author(s): Mineyuki, Y., and Karahara, I
  • Organizer: Andrew Staehelin?s Retirement Symposium
  • Place of Presentation: Boulder, USA
  • Year and Date: 2007-06-06
• [Presentation] 電子線トモグラフィー-分裂準備帯における膜系の3次元微細構造解析- (2007)
  • Author(s): 唐原一郎, 須田甚将, Staehelin, L.A., 峰雪芳宣
  • Organizer: 第48回日本植物生理学会年会シンポジウム"植物細胞を観る??植物バイオイメージング最前線-"
  • Place of Presentation: 松山
• [Presentation] 電子線トモグラフィーによる分裂準備帯に局在する膜系の解析 (2007)
  • Author(s): 唐原 一郎
  • Organizer: 日本顕微鏡学会第63回学術講演会
  • Place of Presentation: 新潟朱鷺メッセ
• [Presentation] Preprophase band microtubules lack capped ends (2007)
  • Author(s): Mineyuki, Yoshinobu
  • Organizer: 日本細胞生物学会第59回年会
  • Place of Presentation: 福岡市
• [Presentation] 細胞分裂パターン制御に関与する細胞骨格・膜からなるナノシステムの解析 (2006)
  • Author(s): 峰雪芳宣
  • Organizer: 第66回 京阪神地区 植物細胞生理学談話会
  • Place of Presentation: 大阪市
  • Year and Date: 2006-03-25
• [Presentation] Electron tomographic analysis of cortical microtubule ends in plant interphase and prophase cells preserved by high-pressure freezing (2006)
  • Author(s): Mineyuki, Y., Karahara, I., Suda, J., and Staehelin, L.A
  • Organizer: The 16th Internatinal Microscopy Congress
  • Place of Presentation: Sapporo
• [Presentation] Effects of cytochalasin D on vesicle transport at the preprophase band analyzed by electron tomography (2006)
  • Author(s): Suda, J., Karahara, I., Staehelin, L.A., and Mineyuki, Y
  • Organizer: The 16th Internatinal Microscopy Congress
  • Place of Presentation: Sapporo
• [Presentation] Electron tomographic analysis of cortical microtubule ends in plant interphase and prophase cells preserved by high-pressure freezing (2006)
  • Author(s): Mineyuki, Y., Karahara, I., Suda, J., and Staehelin, L.A
  • Organizer: The 16th Gordon Research Conference Plant & Fungal Cytoskeleton
  • Place of Presentation: Andover, New Hampshire, USA
• [Presentation] 加圧凍結・電子線トモグラフィーを使った微小管端構造の解析 (2005)
  • Author(s): 峰雪芳宣, 唐原一郎
  • Organizer: 日本植物学会第69回大会シンポジウム"微小管構築の分子機構解明への新展開"
  • Place of Presentation: 富山市
• [Presentation] 加圧凍結・2軸電子線トモグラフィー法による微小管ダイナミクス制御ナノマシンの解析 (2005)
  • Author(s): 峰雪芳宣, 唐原一郎
  • Organizer: 日本顕微鏡学会第61回学術講演会 シンポジウム 第2部"電子線トモグラフィーと細胞生物学"
  • Place of Presentation: つくば市
• [Presentation] Membrane Associated Microtubule System in Lower Land Plants Holds Clues to Evolution of the Cytokinetic Apparatus of Division Site Determination in Flowering Plants (2004)
  • Author(s): Mineyuki, Y., and Shimamura, M
  • Organizer: The Americal Society for Cell Biology 2004 Summer Meeting "Cytokinesis"
  • Place of Presentation: Vermont, USA
• [Book] 電子線トモグラフィーとは何か : ナノスケールでの3D バイオイメージング. 電子顕微鏡で読み解く生命のなぞ ナノワールドに迫るパワフル技術入門 (2008)
  • Author(s): 唐原一郎, 須田甚将, 峰雪芳宣
  • Publisher: 藤本豊士, 山本章嗣編 (東京 : 秀潤社)
• [Book] 電子線トモグラフィー. 細胞工学別冊植物細胞工学シリーズ22 植物の細胞を観る実験プロトコール (2006)
  • Author(s): 峰雪芳宣, 唐原一郎, 須田甚将
  • Publisher: 福田裕穂, 西村幹生, 中野明彦編(秀潤社)
• [Book] Methods in Molecular Biology Series, S. Schwartzbach and T. Osafune, eds(Totoa, NJ : The Humana Press Inc.)(in press)
  • Author(s): Takeuchi, M., Takabe, K., and Mineyuki, Y
  • Publisher: Immunoelectron microscopy of cryofixed and freeze- substituted plant tissues. In Immuno-electron Microscopy : Methods and Protocols
• [Book] Immunoelectron microscopy of cryofixed and freeze- substituted plant tissues. In Methods in Molecular Biology, Immuno-electron Microscopy : Methods and Protocols eds. S. D. Schwartzbach and T. Osafune
  • Author(s): Takeuchi, M.
  • Publisher: The Humana Press Inc.(印刷中)
• [Remarks]
  • URL: http://www.sci.u-hyogo.ac.jp/life/biosynth/index-j.html
• [Remarks]
  • URL: http://www.sci.u-hyogo.ac.jp/life/biosynth/index-j.html
• [Remarks]
  • URL: http://www.sci.u-hyogo.ac.jp/life/biosynth/index-j.html