Assembly mechanism of the highly regular axonemal structure in cilia and flagella

• Project/Area Number: 17207009
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Biophysics
• Research Institution: The University of Tokyo
• Principal Investigator: KAMIYA Ritsu The University of Tokyo, 大学院・理学系研究科, 教授 (10124314)
• Co-Investigator(Kenkyū-buntansha): HIRONO Masafuml 東京大学, 大学院・理学系研究科, 准教授 (10212177) ; YAGI Toshiki 京都大学, 大学院・理学研究科, 准教授 (40292833)
• Project Period (FY): 2005 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥36,400,000 (Direct Cost: ¥28,000,000、Indirect Cost: ¥8,400,000); Fiscal Year 2008: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000); Fiscal Year 2007: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000); Fiscal Year 2006: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000); Fiscal Year 2005: ¥16,380,000 (Direct Cost: ¥12,600,000、Indirect Cost: ¥3,780,000)
• Keywords: 鞭毛 / 繊毛 / 軸糸 / ダイニン / 微小管 / 基底小体 / 中心子 / クラミドモナス / 鞭毛・繊毛 / ネキシンリンク / PACRG / 周辺微小管 / 9+2構造 / カートホイール / SAS-6 / Rib72 / Pacrg / 構造の周期性

Research Abstract

鞭毛・繊毛の内部は9本の周辺微小管が円筒状に並んだ構造を持ち、縦方向には96nmの繰り返し周期がある。このような構造の形成機構に迫るため、緑藻クラミドモナスを使って、軸糸微小管の結合蛋白質を検索するとともに、構造形成異常変異株の原因遺伝子を決定した。その結果、新規軸糸構成蛋白質10種を発見するとともに、軸糸の9回の対称性の起源に関して重要な知見を得た。

Research Products

• [Journal Article] IC97 is a novel intermediate chain of I1 dynein that interacts with tubulin and regulates interdoublet sliding (2009)
  • Author(s): Wirschell, M., Yang, C., Yang, P, Fox, L., Yanigasawa, H., Kamiya, R., Witman, G. B., Porter, M., Sale, W. S.
  • Journal Title: Mot.Biol.Cell 20 Pages: 3044-3054
• [Journal Article] Identification of novel dyneins that localize exclusively to the proximal portion of Chlamydomonas flagella (2009)
  • Author(s): Yagi, T., Uematsu, K., Liu, Z., Kamiya, R.
  • Journal Title: J.Cell Sci. 122 Pages: 1306-1314
• [Journal Article] Ratchetlike properties of in vitro microtubule translocation by a Chlamydomonas inner arm dynein speciesc in the presence of flow (2009)
  • Author(s): Kikushima, K.
  • Journal Title: Biophys.J. 97 Pages: 1657-1662
  • Peer Reviewed
• [Journal Article] Systematic comparison of in vitro motile properties between Chlamydomonas wild-type and mutant outer arm dyneins each lacking one of the three heavy chains (2009)
  • Author(s): Furuta, A.
  • Journal Title: J.Biol.Chem. 284 Pages: 5927-5935
  • Peer Reviewed
• [Journal Article] Identification of novel dyneins that localize exclusively to the proximal portion of Chlamydomonas flagella (2009)
  • Author(s): Yagi, T.
  • Journal Title: J.Cell Sci. 122 Pages: 1306-1314
  • Peer Reviewed
• [Journal Article] IC97 is a novel intermediate chain of 11 dynein that interacts with tubulin and regulates interdoublet sliding (2009)
  • Author(s): Wirschell, M.
  • Journal Title: Mol.Biol.Cell 20 Pages: 3044-3054
  • Peer Reviewed
• [Journal Article] 1IC138 defines a sub-domain at the base of the I1 dynein that regulates microtubule sliding and flagellar motility (2009)
  • Author(s): Bower, R.
  • Journal Title: Mol.Biol.Cell 20 Pages: 3055-3063
  • Peer Reviewed
• [Journal Article] Calcium-dependent flagellar motility activation in Chlamydomonas reinhardtii in response to mechanical agitation (2009)
  • Author(s): Wakabayashi, K.
  • Journal Title: Cell Motil Cytoskeleton 66 Pages: 736-742
  • Peer Reviewed
• [Journal Article] (2009)
  • Author(s): King, S.M., Kamiya, R.
  • Journal Title: Axonemal dyneins : assembly, structure and force generation.(Elsevier Press) Pages: 129-206
• [Journal Article] A novel 44-kDa subunit of axonemal dynein conserved from Chlamydomonas to mammals (2008)
  • Author(s): Yamamoto, R
  • Journal Title: Euk.Cell 7 Pages: 154-161
  • Peer Reviewed
• [Journal Article] Clockwise translocation of microtubules by flagellar inner-armdyneins in vitro (2008)
  • Author(s): Kikushima, K.
  • Journal Title: Biophys.J. 94 Pages: 4014-4019
  • Peer Reviewed
• [Journal Article] Partially functional outer-arm dynein in a novel Chlamydomonas mutant expressing a truncatedg heavy chain (2008)
  • Author(s): Liu, Z.
  • Journal Title: Euk.Cell 7 Pages: 1136-1145
  • Peer Reviewed
• [Journal Article] Ktu/PF13 is required for cytoplasmic pre-assembly of axonemal dyneins (2008)
  • Author(s): Omran, H.
  • Journal Title: Nature 456 Pages: 611-616
  • Peer Reviewed
• [Journal Article] SAS-6 is a cartwheel protein that establishes the 9-fold symmetry of the centriole (2007)
  • Author(s): Nakazawa, Y., Hiraki, M., Kamiya, R., Hirono, M.
  • Journal Title: Curr.Biol 17 Pages: 2169-2174
• [Journal Article] Bldl0p constitutes the distal fiber of the cartwheel, a crucial strucuture that stabilizes the 9-fold microtubule arrangement in the centriole (2007)
  • Author(s): Hiraki, M., Nakazawa, Y., Kamiya, R., Hirono, M.
  • Journal Title: Curr.Biol. 17 Pages: 1778-1783
• [Journal Article] Axonemal localization of Chlamydomonas PACK, a homologue of the human Park in-coregulated gene product (2007)
  • Author(s): Ikeda, K., Ikeda, T., Morikawa, K., Kamiya, R.
  • Journal Title: Cell Motil.Cytoskeleton 64 Pages: 814-821
• [Journal Article] Axonemal localization of Chlamydomonas PACRG, a homologue of the human Parkin-coregulated gene product (2007)
  • Author(s): Ikeda, K.
  • Journal Title: Cell Motil. Cytoskeleton 64 Pages: 814-821
  • Peer Reviewed
• [Journal Article] Bld10p constitutes the cartwheel-spoke tip and stabilizes the 9-fold symmetry of the centriole (2007)
  • Author(s): Hiraki, M.
  • Journal Title: Current Biology 17 Pages: 1778-1783
  • Peer Reviewed
• [Journal Article] SAS-6 is a cartwheel protein that establishes the 9-fold symmetry of the centriole (2007)
  • Author(s): Nakazawa, Y.
  • Journal Title: Current Biology 17 Pages: 2169-2174
  • Peer Reviewed
• [Journal Article] Functional binding of inner-arm dyneins with demembranated flagella of Chlamydomonas mutants (2006)
  • Author(s): Yamamoto, R., Yagi, T., Kamiya, R.
  • Journal Title: Cell Motil.Cytoskeleton 63 Pages: 258-265
• [Journal Article] Radial spoke proteins of Chlamydomonas flagella (2006)
  • Author(s): Yang, P.
  • Journal Title: Journal of Cell Science 119 Pages: 1165-1174
• [Journal Article] Functional binding of inner-arm dyneins with demembranated flagella of Chlamydomonas mutants (2006)
  • Author(s): Yamamoto, R.
  • Journal Title: Cell Motility and the Cytoskeleton 63 Pages: 258-265
• [Journal Article] A novel subunit of axonemal dynein conserved among lower and higher eukaryotes (2006)
  • Author(s): Yamamoto, R.
  • Journal Title: FEBS Lett 580 Pages: 6357-6560
• [Journal Article] The proteins in radial spoke, the mechanochemical signal transducer in 9+2 cilia and flagella (2006)
  • Author(s): Yang, P.
  • Journal Title: Journal of Cell Science (印刷中)
• [Journal Article] Functional binding of inner-arm dyneins with demembranated flagella of Chlamydomonas mutants (2006)
  • Author(s): Yamamoto, R.
  • Journal Title: Cell Motility and the Cytoskeleton (印刷中)
• [Journal Article] An axonemal dynein particularly important for flagellar movement at high viscosity : implications from a new Chlamydomonas mutant deficient in the dynein heavy chain gene Dhc9 (2005)
  • Author(s): Yagi, T., Minoura, I., Fujiwara, A., Saito, R., Yasunaga, T., Hirono, M., Kamiya, R.
  • Journal Title: J.Biol.Chem. 280 Pages: 41412-41420
• [Journal Article] The mouse ortholog of EFHC1 implicated in juvenile myoclonic epilepsy is an axonemal protein widely conserved among organisms with motile cilia and flagella (2005)
  • Author(s): Ikeda, T., Ikeda, K., Enomoto, M., Park, M. K., Hirono, M., Kamiya, R.
  • Journal Title: FEBS Lett. 579 Pages: 819-822
• [Journal Article] Cyclical interactions between two outer doublet microtubules in split flagellar axonemes (2005)
  • Author(s): Aoyama, S.
  • Journal Title: Biophysical Journal 89 Pages: 3261-3268
• [Journal Article] An axonemal dynein particularly important for flagellar movement at high viscosity : implications from a new Chlamydomonas mutant deficient in the dynein heavy chain gene Dhc9 (2005)
  • Author(s): Yagi, T.
  • Journal Title: Journal of Biological Chemistry 280 Pages: 41412-41420
• [Presentation] Importance of tubulin polyglutamylation for cilia/flagella motility as revealed by a novel Chlamydomonas mutant (2009)
  • Author(s): 久保智広
  • Organizer: Gordon Research Conference on Cilia, Mucus, and Mucociliary Interactions
  • Place of Presentation: Lucca, Italy
• [Presentation] Genetic analyses of 9-fold symmetrical structures of the basal body and axoneme (2009)
  • Author(s): Masafumi Hirono
  • Organizer: Gordon Research Conference on Cilia, Mucus, and Mucociliary Interactions
  • Place of Presentation: Lucca, Italy
• [Presentation] クラミドモナス変異体で探る繊毛の形成と運動機構 (2008)
  • Author(s): 神谷律
  • Organizer: 第113回日本解剖学会総会
  • Place of Presentation: 大分
  • Year and Date: 2008-03-28
• [Presentation] SAS-6 is crucial for establishing "nine-ness" of the centriole (2008)
  • Author(s): 中澤友紀
  • Organizer: 第60回日本細胞生物学会大会
  • Place of Presentation: 横浜
• [Presentation] A novel coiled-coil protein that localizes exclusively to immature centrioles in Chlamydomonas (2008)
  • Author(s): 白土玄
  • Organizer: 第60回日本細胞生物学会大会
  • Place of Presentation: 横浜
• [Presentation] クラミドモナス変異株で明らかになった鞭毛チューブリン翻訳後修飾の機能的重要性 (2008)
  • Author(s): 久保智広
  • Organizer: 日本動物学会第79回大会
  • Place of Presentation: 福岡
• [Presentation] Phenotypic analyses of novel mutants lacking an intermediate chain of flagellar inner-arm dyneinf(I1) (2008)
  • Author(s): 山本遼介
  • Organizer: 第46回日本生物物理学会年会
  • Place of Presentation: 福岡
• [Presentation] Motile properties of Chlamydomonas outer arm dynein heavy chains studied in vitro (2008)
  • Author(s): 古田茜
  • Organizer: 第46回日本生物物理学会年会
  • Place of Presentation: 福岡
• [Presentation] Novel inner-arm dyneins localized to the proximal portion of the Chlamydomonas flagella (2008)
  • Author(s): 八木俊樹
  • Organizer: 第46回日本生物物理学会年会
  • Place of Presentation: 福岡
• [Presentation] Identification of Three Minor Inner-Arm Dynein Heavy Chains in Chlamydomonas flagella (2008)
  • Author(s): 八木俊樹
  • Organizer: 13th International Chlamydomonas Conference
  • Place of Presentation: Hyeres-les-Palmiers, France
• [Presentation] Structural and Biochemical Properties of the Outer-Dynein-Arm Docking Complex(ODA-DC)Studied Using Recombinant Proteins (2008)
  • Author(s): Ken-ichi Wakabayashi
  • Organizer: 13th International Chlamydomonas Conference
  • Place of Presentation: Hyeres-les-Palmiers, France
• [Presentation] Mechanism for establishing the 9-fold symmetry of the centriole structure A role of the cartwheel (2008)
  • Author(s): Masafumi Hirono
  • Organizer: 13th International Chlamydomonas Conference
  • Place of Presentation: Hyeres-les-Palmiers, France
• [Presentation] Assembly of the nine-fold symmetrical structure of basal bodies/centrioles (2008)
  • Author(s): Masafumi Hirono
  • Organizer: Gordon Research Conference on Plant and Fungal Cytoskeleton
  • Place of Presentation: Lucca, Italy
• [Presentation] A cartwheel-dependent mechanism for establishing the centriolar'nine-ness (2008)
  • Author(s): Masafumi Hirono
  • Organizer: First EMBO Conference on Centrosomes and Spindle Pole Bodies
  • Place of Presentation: Heidelberg, Germany
• [Presentation] Formation of Abnormal Centrioles with Eight-fold Symmetry in Chlamydomonas Cells Expressing Truncated Bld10p, a Component of the Centriolar Cartwheel (2007)
  • Author(s): Hiraki, M.
  • Organizer: 日本細胞生物学会第59回大会
  • Place of Presentation: 福岡
• [Presentation] 中心子の三連微小管数が揺らぐクラミドモナス突然変異株bld12の変異遺伝子 (2007)
  • Author(s): 中澤友紀
  • Organizer: 日本動物学会第78回大会
  • Place of Presentation: 弘前
• [Presentation] クラミドモナス蛋白質Bld10pの部分配列発現による8回対称性中心子の形成 (2007)
  • Author(s): 平木まどか
  • Organizer: 日本動物学会第78回大会
  • Place of Presentation: 弘前
• [Presentation] ネキシンリンクの実体 (2007)
  • Author(s): 柳澤春明
  • Organizer: 日本動物学会第78回大会
  • Place of Presentation: 弘前
• [Presentation] A Chlamydomonas Outer Arm Dynein Mutant with a Truncated Gamma Heavy Chain (2007)
  • Author(s): Liu Z.
  • Organizer: 日本生物物理学会第45回年会
  • Place of Presentation: 横浜
• [Presentation] Motile Properties of Chlamydomonas Outer Arm Dynein Studied Using a Novel Labeling Method (2007)
  • Author(s): Okawa, A.
  • Organizer: 日本生物物理学会第45回年会
  • Place of Presentation: 横浜
• [Presentation] Identification of Three Minor Inner-Arm Dynein Heavy Chains in Chlamydomonas flagella (2007)
  • Author(s): Yagi, T.
  • Organizer: 日本生物物理学会第45回年会
  • Place of Presentation: 横浜
• [Presentation] Diversity of Chlamydomonas Axonemal Dyneins : Novel Species and Novel Motor Properties (2007)
  • Author(s): Yagi, T.
  • Organizer: 2007 FASEB Summer Research Conferences Biology of Cilia and Flagella
  • Place of Presentation: Saxtons River, Vermont, USA
• [Presentation] Centrioles with Aberrant Numbers of Triplet Microtubules Formed in a Novel Chlamydomonas Mutant that Lacks the Centriolar Cartwheel (2007)
  • Author(s): Nakazawa, Y.
  • Organizer: 2007 FASEB Summer Research Conferences Biology of Cilia and Flagella
  • Place of Presentation: Saxtons River, Vermont, USA
• [Presentation] Structural and biochemical properties of the outer-dynein-arm docking complex (ODA-DC) Studied Using Recombinant Proteins (2007)
  • Author(s): Wakabayshi, K.
  • Organizer: 2007 FASEB Summer Research Conferences Biology of Cilia and Flagella
  • Place of Presentation: Saxtons River, Vermont, USA
• [Presentation] Identification of novel subunits of inner arm dynein conserved among various eukaryotes (2007)
  • Author(s): Yamamoto, R.
  • Organizer: 2007 FASEB Summer Research Conferences Biology of Cilia and Flagella
  • Place of Presentation: Saxtons River, Vermont, USA
• [Presentation] In Vitro Motility of chlamydomonas outer arm dynein studied using a novel labeling method (2007)
  • Author(s): Okawa, A.
  • Organizer: 2007 FASEB Summer Research Conferences Biology of Cilia and Flagella
  • Place of Presentation: Saxtons River, Vermont, USA
• [Presentation] Novel species and novel motor properties of axonemal dyneins (2007)
  • Author(s): Yagi, T.
  • Organizer: International Conference Molecular Mechanism of Intracellular Transport : The Roles of Kinesin and Dynein Superfamily Proteins
  • Place of Presentation: Tomakomai, Japan
• [Presentation] High-speed inter-microtubule sliding driven by aligned axonemal outer-arm dynein (2007)
  • Author(s): Aoyama, S.
  • Organizer: nternational Conference Molecular Mechanism of Intracellular Transport : The Roles of Kinesin and Dynein Superfamily Proteins
  • Place of Presentation: Tomakomai, Japan
• [Presentation] Identification of the Genes of All Known Chlamydomonas Inner-Arm Dynein Heavy Chains (2006)
  • Author(s): Yagi, T., Kamiya, R.
  • Organizer: 46th Annual Meeting of the American Society for Cell Biology
  • Place of Presentation: San Diego, USA
• [Presentation] A novel Chlamydomonas mutant that assembles flagellar axonemes with variable numbers of outer doublet microtubules (2006)
  • Author(s): Nakazawa, Y., Kamiya, R., Hirono, M.
  • Organizer: 12th International Conference on the Cell and Molecular Biology of Chlamydomonas
  • Place of Presentation: Portland, Oregon, USA
• [Presentation] Novel longitudinal compartmentalization in the axoneme revealed by localization of putative interdoublet link components (2006)
  • Author(s): Yanagisawa, H., Kamiya, R.
  • Organizer: 12th International conference on the Cell and Molecular Biology of Chlamydomonas
  • Place of Presentation: Portland, Oregon, USA
• [Book] Axonemal dyneins : assembly, structure and force generation. In G. B. Witman (ed.), Chlamydomonas source book, vol. III (2009)
  • Author(s): King, S. M., Kamiya, R.
  • Publisher: Elsevier Press
• [Remarks]
  • URL: http://www.biol.s.u-tokyo.ac.jp/users/seiri/lab.html