A large-scale screening of Minos-inserted mutants and analysis of genes involved in the mutation in the chordate Clone intestinalis
| Project/Area Number |
17207013
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Developmental biology
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| Research Institution | Kyoto University |
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Principal Investigator |
SATOH NORIYUKI Kyoto University, Graduate School of Science, Professor (30025481)
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| Co-Investigator(Kenkyū-buntansha) |
SASAKURA Yasunori University of Tsukuba, Graduate School of Life and Environmental Sciences, Lecturer (10400649)
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| Project Period (FY) |
2005 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥51,480,000 (Direct Cost: ¥39,600,000、Indirect Cost: ¥11,880,000)
Fiscal Year 2007: ¥15,080,000 (Direct Cost: ¥11,600,000、Indirect Cost: ¥3,480,000)
Fiscal Year 2006: ¥15,080,000 (Direct Cost: ¥11,600,000、Indirect Cost: ¥3,480,000)
Fiscal Year 2005: ¥21,320,000 (Direct Cost: ¥16,400,000、Indirect Cost: ¥4,920,000)
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| Keywords | Ciona intestinalis / developmental genetics / insertional mutagenesis / development and differentiation / function of genes / a large-scale screening / microarray / metamorphosis / 大槻模スクリーニング / 発生遺伝子 / 機能解析 / swimming juvenile / セルロース合成酵素遺伝子 |
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| Research Abstract |
Ciona intestinalis offer the promise of forward-genetics since their life cycle is relatively short, 8-12 weeks from fertilization to Fl embryos, and it is possible to induce self-fertilization within hermaphrodites. The compact genome may also offer opportunity to accomplish mutations leading to visible phenotypes. In Ciona, successful germ-line transgenesis with the Tc1/mariner superfamily transposon, Minos, has been established by ourselves. The aim of the present study is to isolated Minos-inserted mutants as many as possible, and to identify genes that are responsible for the mutation. The present study is also intended to introduce various transgenic techniques into the Ciona system. During the period of 2005-2008, we have isolated approximately 50 mutation lines and 20 transgenic lines. The mutation lines include swimming juveniles (sj) 1-4, balloon (bal), and tail regression failed (trf). All these mutants showed abnormal process of metamorphosis. Interestingly, the sj- I mutant phenotype arises form the insertion of Minos into the promoter region of Ci-CesA, a gene encoding cellulose synthase. We are now analyzing molecular mechanisms involved in the mutations using microarray technique. Transgenic lines include those with GFP expression in embryonic muscle, notochord, or nervous system. These provide useful marker lines for further studies of the expression and function of chordate genes.
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Report
(4 results)
Research Products
(35 results)
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[Journal Article] Transposon-mediated transgenesis in a marine invertebrate chordate, Ciona intestinalis2007
Author(s)
Sasakura, Y., Oogai, Y., Matsuoka, T., Satoh, N., Awazu, S
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Journal Title
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Culture of Ciona intestinalis in closed systems2007
Author(s)
Joly, J., Kano, S., Matsuoka, T., Auger, H., Hirayama, K., Satoh, N., Awazu, S., Legendre, L., Sasakura, Y
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Journal Title
Developmental Dynamics 236
Pages: 1832-1841
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Urochordate Genomes2006
Author(s)
Satoh, N., Kawashima, T., Shoguchi, E., Satou, Y
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Journal Title
Vertebrate Genomes, Volff, J-N (ed), Karger, Basal
Pages: 198-212
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] J-i, and Satoh, N. Transposon-mediated insertional mutagenesis revealed the functions of animal cellulose synthase in the ascidian Ciona intestinalis2005
Author(s)
Sasakura, Y., Nakashima, K., Awazu, S., Matsuoka, T., Nakayama, A., Azuma
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Journal Title
Proc. Natl. Acad. Sci., USA 102
Pages: 15134-15139
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Transposon-mediated mutagenesis reveals the function of Alpha-2-macroglobulin in Ciona intestinalis2006
Author(s)
Matsuoka, T., Awazu, S., Inaba, K., Satoh, N., Sasakura, Y
Organizer
39th Annual Meeting of the Japanese Society of Developmental Biologists
Place of Presentation
Hiroshima, Japan
Description
「研究成果報告書概要(欧文)」より
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