Molecular mechanism of sex determination in fish

• Project/Area Number: 17208019
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General fisheries
• Research Institution: University of the Ryukyus
• Principal Investigator: NAKAMURA Masaru University of the Ryukyus, 熱帯生物圏研究センター, 教授 (10101734)
• Co-Investigator(Kenkyū-buntansha): KOBAYASHI Tohru 愛媛大学, 南予水産研究センター, 准教授 (30221972) ; HIRAI Toshiaki 帝京科学大学, 理工学部, 講師 (30238331)
• Project Period (FY): 2005 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥46,800,000 (Direct Cost: ¥36,000,000、Indirect Cost: ¥10,800,000); Fiscal Year 2008: ¥10,530,000 (Direct Cost: ¥8,100,000、Indirect Cost: ¥2,430,000); Fiscal Year 2007: ¥9,620,000 (Direct Cost: ¥7,400,000、Indirect Cost: ¥2,220,000); Fiscal Year 2006: ¥10,010,000 (Direct Cost: ¥7,700,000、Indirect Cost: ¥2,310,000); Fiscal Year 2005: ¥16,640,000 (Direct Cost: ¥12,800,000、Indirect Cost: ¥3,840,000)
• Keywords: 性分化 / 性転換 / 性決定 / 雌性ホルモン / 雄性ホルモン / アロマターゼ / ステロイド代謝酵素 / 生殖細胞 / ステイロイド代謝酵素 / 生物細胞

Research Abstract

魚類の性決定機構の解明を組織学的、免疫組織学的、細胞学的及び分子生物学的手法を用いて行った。その結果、雌雄異体魚の成熟卵巣を精巣へと転換させることに成功した。性決定に雌性ホルモンと雄性ホルモンのバランスの変化が重要な役割を果たしていることが明らかにした。更に、性決定には脳からの刺激である生殖腺刺激ホルモン及び生殖腺内ではそのシグナルを受け取る生殖腺刺激ホルモン受容体が重要であることを明らかにした。

Research Products

• [Journal Article] Molecular characterization and quantification of the gonadotropin receptors FSH-R and LH-R from Atlantic cod(Gadus morhua). (2009)
  • Author(s): Mittelholzer, C
  • Journal Title: Gen. Comp. Endocrinol. 106 Pages: 47-58
  • Peer Reviewed
• [Journal Article] Immunohistochemical evidence for 11 β-hydroxylase(P45011 β)and androgen production in the gonad during sex differentiation and in adults in the protandrous anemonefish Amphiprion clarkii (2008)
  • Author(s): S. Miura, R. Horiguchi, M. Nakamura
  • Journal Title: Zool Sci 25 Pages: 212-219
  • NAID: 40016029293
  • Peer Reviewed
• [Journal Article] Sexual dimorphic expression of genes in gonads during early differentiation of a Teleost fish, the nile tilapia (2008)
  • Author(s): S. Ijiri, T. Kobayashi, M. Nakamura(他5人)
  • Journal Title: Oreochromis niloticus.Biology of reproduction 78 Pages: 333-341
  • Peer Reviewed
• [Journal Article] Sex change of adult initial-phase male wrasse, Halichoeres trimaculatus by estradiol-17β treatment (2008)
  • Author(s): Y. Kojima, R.K. Bhandari, T. Kobayashi, M. Nakamura
  • Journal Title: Gen Comp Endocrinol 156 Pages: 628-632
  • Peer Reviewed
• [Journal Article] Appearances and chronological changes of mummichog Fundulus heteroclitus FSH cells and LH cells curing ontogeny, sexual differentiation, and gonadal development (2008)
  • Author(s): A. Shimizu, T. Kobayashi, M. Nakamura(他5人)
  • Journal Title: Gen Comp Endocrinol 156 Pages: 312-322
  • Peer Reviewed
• [Journal Article] Differentiation of ambisexual gonads and immunohistochemical localization of P450 cholesterol side-chain cleavage enzyme during gonadal sex differentiation in the protandous anemonefish (2008)
  • Author(s): S. Miura, S. Nakamura, Y. Kobayashi, P. Francesc, M. Nakamura
  • Journal Title: Amphiprion clarkii. Comp. Biochem. Physiol B149 Pages: 29-37
  • Peer Reviewed
• [Journal Article] Molecular cloning and expression of three estrogen receptors in the protogynous wrasse (2008)
  • Author(s): R. Horiguchi, Y. Kobayashi and M. Nakamura
  • Journal Title: Halichoeres trimaculatus. Cybium 32(2) Pages: 88-99
  • Peer Reviewed
• [Journal Article] In vitro induction of gonadal sex change in a protogynous fish, three-spotted wrasse (2008)
  • Author(s): T. Todo, M. Nakamura(他6人)
  • Journal Title: (Halichores trimaculatus). Cybium 32(2) Pages: 106-106
  • Peer Reviewed
• [Journal Article] Post-sex differentiational' sex reversal in the female common carp (2008)
  • Author(s): S. Ogawa, M. Akiyoshi, M. Higuchi, M. Nakamura, T. Hirai
  • Journal Title: (Cyprinus carpio). Cybium 32(2) Pages: 102-103
  • Peer Reviewed
• [Journal Article] The role of estrogen in gonadal sex differentiation in protandrous anemonefish Amphiprion clarkii (2008)
  • Author(s): S. Miura, M. Nakamura
  • Journal Title: Immunohistochemical localization of P450scc in gonads of male and female phases. Cybium 32(2) Pages: 87-87
  • Peer Reviewed
• [Journal Article] Molecular cloning and quantitative expression of sexually dimorphic markers Dmrt1 and Foxl2 during female-to-male sex change in Epinephelus merra (2008)
  • Author(s): M .A. Alam, Y. Kobayashi, R. Horiguchi, T. Hirai, M. Nakamura
  • Journal Title: Gen Comp Endocrinol 157 Pages: 75-85
  • Peer Reviewed
• [Journal Article] Determination of sex and gonadal maturity in the honeycomb grouper (2008)
  • Author(s): M.A. Alam, M. Nakamura
  • Journal Title: Epinephelus merra, through biopsy. Aquacult. Int 16 Pages: 27-32
  • Peer Reviewed
• [Journal Article] Phylogenetic diversity and co-symbiosis in the bioluminescent symbioses of Photobacterium mandapamenis (2008)
  • Author(s): A.J. Kaeding, M. Nakamura 他6人
  • Journal Title: Appl Environ Microbiol Pages: 3173-3182
  • Peer Reviewed
• [Journal Article] Stability in aromatase immunoreactivity of steroid-producing cells during early Development of XX gonads of the Nile tilapia (2008)
  • Author(s): F. Sakai, T. Kobayashi, M. Matsuda and Y.Nagahama
  • Journal Title: Oreochromis niloticus : An organ culture Study. Zool. Sci 25(3) Pages: 344-348
  • Peer Reviewed
• [Journal Article] Sexual dimorphic expression of DMRT1 and Sox9 during gonadal differentiation and hormone-induced sex reversal in the teleost fish Nile tilapia(Oreochromis niloticus) (2008)
  • Author(s): T. Kobayashi, H. Kajiura-Kobayashi, G. Guan and Y. Nagahama
  • Journal Title: Dev. Dyn 237(1) Pages: 297-306
  • Peer Reviewed
• [Journal Article] Molecular Cloning of Plk1 and Nek2 and their Expression in Mature Gonads of the Teleost Fish Nile Tilapia(Oreochromis Niloticus) (2008)
  • Author(s): Y. Matsuoka, T. Kobayashi, K. Kihara and Y. Nagahama
  • Journal Title: Mol. Reprod. Develop 75(6) Pages: 989-1001
  • Peer Reviewed
• [Journal Article] Seabream GnRH immunoreactivity in brain and pituitary of XX and XY Nile tilapia, (2008)
  • Author(s): I. Swapna, T. Kobayashi(他6人)
  • Journal Title: Oreochroomis niloticus during early development. J. Exp. Zool., Part A 309(7) Pages: 419-426
  • Peer Reviewed
• [Journal Article] Molecular cloning and gene expression of Foxl2 in the frog Rana rugosa. Gen (2008)
  • Author(s): Y. Oshima, Y. Uno, Y. Matsuda, T. Kobayashi and M. Nakamura
  • Journal Title: Comp. Endocrinol 159(2-3) Pages: 170-177
  • Peer Reviewed
• [Journal Article] Transgenic medaka enables easy detection of oocytes in live fish (2008)
  • Author(s): M. Kinoshita, T. Kobayashi(他6人)
  • Journal Title: Mol. Reprod. Develop 76(2) Pages: 202-207
  • Peer Reviewed
• [Journal Article] . Immunohistochemical evidence for 11β-hydroxylase(P45011β)and androgen production in the gonad during sex defferentiation and in adults in the protandrous anemonefish Amphiprion clarkii. (2008)
  • Author(s): S. Miura
  • Journal Title: Zool Sci 25 Pages: 212-219
  • Peer Reviewed
• [Journal Article] Sexual dimorphic expression of genes in gonads during early differentiation of a Teleostfish, the nile tilapia Oreochromis niloticus. (2008)
  • Author(s): S. Ijiri
  • Journal Title: Biology of reproduction 78 Pages: 333-341
  • Peer Reviewed
• [Journal Article] Sex change of adult initial-phase male wrasse, Halichoeres trimaculatus by estradiol-17 β treatment. (2008)
  • Author(s): Y. Kojima
  • Journal Title: Gen Comp Endocrinol 156 Pages: 628-632
  • Peer Reviewed
• [Journal Article] Appearances and chronological changes of mummichog Fundulus heteroclitus FSH cells and LH cells curing ontogeny, sexual differentiation, and gonadal development. (2008)
  • Author(s): A. Shimizu
  • Journal Title: Gen Comp Endocrinol 156 Pages: 312-322
  • Peer Reviewed
• [Journal Article] Differentiation of ambisexual gonads and immunohistochemical localization of P450 cholesterol side-chain cleavage enzyme during gonadal sex differentiation in theprotandrous anemonefish, Amphiprion clarkii (2008)
  • Author(s): S. Miura
  • Journal Title: Comp. biochem. physiol B149 Pages: 29-37
  • Peer Reviewed
• [Journal Article] Molecular cloning and expression of three estrogen receptors in the protogynous wrasse, Halichoeres trimaculatus. (2008)
  • Author(s): R. Horiguchi
  • Journal Title: Cybium 32(2) Pages: 88-89
  • Peer Reviewed
• [Journal Article] In vitro induction of gonadal sex change in a protogynous fish, three-spotted wrasse(Halichoeres trimaculatus). (2008)
  • Author(s): T. Todo
  • Journal Title: Cybium 32(2) Pages: 106-106
  • Peer Reviewed
• [Journal Article] ‘Post-sex differentiational'sex reversal in the female common carp(Cyprinus carpio). (2008)
  • Author(s): S. Ogawa
  • Journal Title: Cybium 32(2) Pages: 102-103
  • Peer Reviewed
• [Journal Article] The role of estrogen in gonadal sex differentiation in protandrous anemonefish Amphiprion clarkii. Immunohistochemical localization of P450scc in gonads of male and female phases. (2008)
  • Author(s): S. Miura
  • Journal Title: Cybium 32(2) Pages: 87-87
  • Peer Reviewed
• [Journal Article] Stability in Aromatase Immunoreactivity of Steroid-Producing Cells during Early Development of XX Gonads of the Nile tilapia, Oreochromis niloticus : An Organ Culture Study・ (2008)
  • Author(s): F. Sakai
  • Journal Title: Zool. Sci 25(3) Pages: 344-348
  • Peer Reviewed
• [Journal Article] Sexual dimorphic expression of DMRT1 and Sox9 during gonadal differentiation and hormone-induced sex reversal in the teleost fish Nile tilapia(Oreochromis niloticus). (2008)
  • Author(s): T. Kobayashi
  • Journal Title: Dev. Dyn 237(1)2 Pages: 297-306
  • Peer Reviewed
• [Journal Article] Molecular Cloning of Plkl and Nek2 and their Expression in Mature Gonads of the Teleost Fish Nile Tilapia (Oreochromis Niloticus). (2008)
  • Author(s): Y. Matsuoka
  • Journal Title: Mol. Reprod. Develop 75 (6) Pages: 989-1001
  • Peer Reviewed
• [Journal Article] Seabream GnRH immunoreactivity in brain and pituitary of XX and XY Nile tilapia, Oreochroomis niloticus during early development. (2008)
  • Author(s): Swapna, I
  • Journal Title: J. Exp. Zool. Part A 309 (7) Pages: 419-426
  • Peer Reviewed
• [Journal Article] Molecular cloning and gene expression of Foxl2 in the frog Rana rugosa. (2008)
  • Author(s): Oshima, Y
  • Journal Title: Gen. Comp. Endocrinol 159 (2-3) Pages: 170-177
  • Peer Reviewed
• [Journal Article] Transgenic medaka enables easy detection of oocytes in live fish. (2008)
  • Author(s): Kinoshita, M
  • Journal Title: Mol. Reprod. Develop 76 (2) Pages: 202-207
  • Peer Reviewed
• [Journal Article] The mechanisms of gonadal sex determination and differentiation in fish (2008)
  • Author(s): Kobayashi, T
  • Journal Title: Bull. Fish. Res Agen No. 26 Pages: 107-114
  • NAID: 40016584265
  • Peer Reviewed
• [Journal Article] Sexual dimorphic expression of genes in gonads during early differentiation of a teleost fish, the Nile tilapia, Oreochromis niloticus. (2008)
  • Author(s): Ijiri, A
  • Journal Title: Biology of Reproduction 78 Pages: 333-341
  • Peer Reviewed
• [Journal Article] Sexual dimorphic expression of DMRT1 and Sox9a during gonadal sex differentiation and hormone-induced sex-reversal in the teleost fish Niletilapia (Oreochromis niloticus). (2008)
  • Author(s): Kobayashi, T.,
  • Journal Title: Developmental Dynamics 237 Pages: 297-306
  • Peer Reviewed
• [Journal Article] Appearances and chronological changes of mummichog Fundulus heteroclitus FSH cells and LH cells during ontogeny, sexual differentiation, and gonadal development (2008)
  • Author(s): Akio Shimizu
  • Journal Title: General and Comparative Endocrinology 156 Pages: 312-322
  • Peer Reviewed
• [Journal Article] Differentiation of ambisexual gonads and immunohistochemical localization of P450 cholesterol side-chain cleavage enzyme during gonadal sex differentiation in the protandrous anemonefish, Amphiprion clarkii (2008)
  • Author(s): Saori Miura
  • Journal Title: Comparative Biochemistry and Physiology 149 Pages: 29-37
  • Peer Reviewed
• [Journal Article] 沖縄の魚の性転換 (2007)
  • Author(s): 中村將
  • Journal Title: 日本病態生理学会雑誌 16 Pages: 25-281
  • Peer Reviewed
• [Journal Article] Nakamura, Efferent duct differentiation during female-tomale sex change in honeycomb grouper Epinephelus merra (2007)
  • Author(s): Alam M.A. and M. Nakamura
  • Journal Title: J Fish Biol 71 Pages: 1-11
  • Peer Reviewed
• [Journal Article] Role of sex hormones in sex change of grouper (2007)
  • Author(s): M. Nakamura, M.A. Alam, Y. Kobayashi, R.K. Bhandari
  • Journal Title: Journal of Marine Science and Technology, Special Issue Pages: 23-27
  • Peer Reviewed
• [Journal Article] Phylogenetic diversity and co-symbiosis in the Bioluminescent Symbioses of Photobacterium mandapamensis Appl (2007)
  • Author(s): J.K. Alison, M. Nakamura(他6人)
  • Journal Title: Environ. Microbiol : 10.1128/AEM Pages: 2212-6
  • Peer Reviewed
• [Journal Article] Foxl2 up-regulates aromatase gene transcription female-specifically by binding to the promoter as well as interacting with Ad4BP/SF-1 (2007)
  • Author(s): D.S. Wang, T. Kobayashi, L.Y. Zhou, B. Paul-Prasanth, S. Ijiri, F. Sakai, K. Okubo, K.I. Morohashi and Y. Nagahama.
  • Journal Title: Mol. Endocrinol 21(3) Pages: 712-725
  • Peer Reviewed
• [Journal Article] DMY induces male development in XX medaka fish. Proc. Natl (2007)
  • Author(s): M. Matsuda, T. Kobayashi(他8人)
  • Journal Title: Natl. Acad. Sci. USA 104(10) Pages: 3865-3870
  • Peer Reviewed
• [Journal Article] A novel type of p450c17 lacking the lyase activity is responsible for c21-steroid biosynthesis in the fish ovary and head kidney (2007)
  • Author(s): L.Y. Zhou, T. Kobayashi(他6人)
  • Journal Title: Endocrinology 148(9) Pages: 4282-4291
  • Peer Reviewed
• [Journal Article] A novel type of P450c17 lacking the lyase activity is responsible for C21-steroid biosynthesis in the fish ovary and head kidney. (2007)
  • Author(s): Zhou, L. Y.,
  • Journal Title: Endocrinology. 148 Pages: 428-491
  • Peer Reviewed
• [Journal Article] Follicle-stimulating hormone signaling and Foxl2 are involved in trans criptional regulation of aromatase gene during gonadal sex differentiation in Japanese flounder, Paralichthys olivaceus. (2007)
  • Author(s): Toshiya Yamaguchi
  • Journal Title: Biochemical and Biophysical Research Communications 359 Pages: 935-940
  • Peer Reviewed
• [Journal Article] Determination of sex and gonadal maturity in the honeycomb grouper, Epinephelus merra, through biopsy. (2007)
  • Author(s): Alam M. A
  • Journal Title: Aquaculture International 16 Pages: 27-32
  • Peer Reviewed
• [Journal Article] Role of sex hormones in sex change of grouper (2007)
  • Author(s): Masaru Nakamura
  • Journal Title: Journal of Marine Science and Technology Pages: 23-27
  • Peer Reviewed
• [Journal Article] Suppression of steroidogenic enzyme expression during androgen-induced sex reversal in Nile tilapia(Oreochromis niloticus) (2006)
  • Author(s): K.B. Ramji, M. Nakamura, T. Kobayashi, and Y. Nagahama
  • Journal Title: Gen Comp Endocrinol 145 Pages: 20-24
  • Peer Reviewed
• [Journal Article] Sex change in coral reef fish (2006)
  • Author(s): M. Nakamura, Y. Kobayashi, S. Miura, M.A. Alam, K.B. Ramji
  • Journal Title: Fish Physiol Biochem 31 Pages: 117-122
  • Peer Reviewed
• [Journal Article] Changes in androgen-producing cell size and circulation 11-ketotestoterone level during famal-male sex change of honycomb grouper epinephelus merra (2006)
  • Author(s): M.A. Alam, R.K. Bhandari, Y. Kobayashi, S. Nakamura, K. Soyano and M. Nakamura
  • Journal Title: 73 Pages: 206-214
  • Peer Reviewed
• [Journal Article] Induction of sex change within two full moons during breeding season and spawning in grouper (2006)
  • Author(s): M.A. Alam, R.K. Bhandari, Y. Kobayashi, K. Soyano, M. Nakamura
  • Journal Title: Aquaculture 255 Pages: 532-535
  • Peer Reviewed
• [Journal Article] GnRHa-accelerated spermatogenesiss in the testes of underyearling golden rabbitfish (2006)
  • Author(s): T. Komatsu, R.K. Bhandari, Y. Kobayashi, S. Nakamura, M. Nakamura
  • Journal Title: Siganus guttatus(Bloch). Aquaculture 257 Pages: 558-565
  • Peer Reviewed
• [Journal Article] A sex cord-like structure and some remarkable features in early gonadal sex differentiation in the marine teleost (2006)
  • Author(s): T. Komatsu, S. Nakamura and M. Nakamura
  • Journal Title: Siganus guttatus(Bloch). J Fish Biol 68 Pages: 236-250
  • Peer Reviewed
• [Journal Article] Induction of female-to-male sex change in the Honeycomb grouper(Epinephelus merra)by 11-ketotestosterone treatments (2006)
  • Author(s): Ramji, K.B., Alam. M.A., K. Soyano and M. Nakamura
  • Journal Title: Zoological Science 23 Pages: 65-69
  • NAID: 110004775625
  • Peer Reviewed
• [Journal Article] Knock-down of DMY initiates female pathway in the genetic male medaka (2006)
  • Author(s): B. Paul-Prasanth, M. Matsuda, E-L. Lau, A. Suzuki, F. Sakai, T. Kobayashi, and Y. Nagahama
  • Journal Title: Oryzias latipes. Biochem. Biophys. Res. Commun 351(4) Pages: 815-519
  • Peer Reviewed
• [Journal Article] サンゴ礁魚類の性分化、性転換機構の形態学的、生理学的研究 (2006)
  • Author(s): 中村將・小林靖尚・三浦さおり
  • Journal Title: Morphological and physiologyical studies on sex differentiation and sex change in coralreef fish.(水産総合研究センター研究報告別冊4号別刷)
  • Peer Reviewed
• [Journal Article] A sex cord-like structure and some remarkable features in early gonadal sex differentiation in a marine teleost, golden rabbitfish Siganus guttatus(Bloch). (2006)
  • Author(s): Komatsu T, Nakamura S, Nakamura M.
  • Journal Title: Journal of Fish Biology 68 Pages: 236-250
• [Journal Article] Suppression of steroidogenic enzyme expression during androgen-induced sex reversal in Nile tilapia (Oreochromis niloticus) (2006)
  • Author(s): Bhandari R.K.Nakamura M., Kobayashi T., Nagahama Y
  • Journal Title: General Comparative Endocrinology 145 Pages: 20-24
• [Journal Article] Promoter analysis of two aromatase genes in the serial-sex changing gobiid fish (2005)
  • Author(s): Y. Kobayashi, T. Sunobe, T. Kobayashi, Y. Nagahama, M. Nakamura
  • Journal Title: Trimma Okinawae. Fish Physiol Biochem 31 Pages: 123-127
  • Peer Reviewed
• [Journal Article] Changes in serum steroid hormones during ovarian sevelopment in the captive common Japanese Conger Conger myriaster(Brevoort) (2005)
  • Author(s): H. Chiba, S. Ijiri, M. Iwata, M. Nakamura, S. Adachi and K. Yamauchi
  • Journal Title: Aquaculture Science 53(2) Pages: 189-198
  • Peer Reviewed
• [Journal Article] Aromatase immunoreactivity and the role of enzymes in steroid pathways for inducing sex change in the hermaphrodite gobiid fish Trimma Okinawae (2005)
  • Author(s): T. Sunobe, M. Nakamura, Y. Kobayashi, T. Kobayashi and Y. Nagahama
  • Journal Title: Comp. Biochem. Physiol. Part A 141 Pages: 54-59
  • Peer Reviewed
• [Journal Article] Immunohistochemical evidence possibly identifying the site of androgen production in the ovary of the protogynous grouper Epinephelus merra (2005)
  • Author(s): M.A. Alam, H. Komuro, R.K. Bhandari, S. Nakamura, K. Soyano, M. Nakamura
  • Journal Title: Cell Tissue Res 320 Pages: 323-329
  • Peer Reviewed
• [Journal Article] Gonadal structure and P450scc and 3β-HSD-like immunoreactivity in the gobiid fish Trimma okinawae during bidirectional sex change (2005)
  • Author(s): T. Sunobe, M. Nakamura, Y. Kobayashi, T. Kobayashi and Y. Nagahama
  • Journal Title: Ichyiol Res 52 Pages: 27-32
  • Peer Reviewed
• [Journal Article] Gonadal structure of the serial-sex changing fish Trimma okinawae (2005)
  • Author(s): Kobayashi Y, Sunobe T, Kobayashi T, Nagahama Y and M Nakamura
  • Journal Title: Dev Grouth Differ 47 Pages: 7-13
  • Peer Reviewed
• [Journal Article] Evidence that estrogen regulates the sex change of honeycomb grouper(Epinephelus merra), a protogynous hermaphrodite fish (2005)
  • Author(s): R.K. Bhandari, M.A. Alam, M. Higa, K. Soyano and M. Nakamura
  • Journal Title: J Exp Zool 303A Pages: 497-503
  • Peer Reviewed
• [Journal Article] Molecular cloning and expression of Ad4BP/SF-1 in the serial sex changing goiid fish Trimma okinawae (2005)
  • Author(s): Y. Kobayashi, T. Sunobe, T. Kobayashi, M. Nakamura, N. Suzuki and Y. Nagahama
  • Journal Title: BBRC 332 Pages: 1073-1080
  • Peer Reviewed
• [Journal Article] Cloning of cDNAs and the differential expression of A-type cyclins and Dmc1 durinspermatogenesis in the Japanese eel, a teleost fish (2005)
  • Author(s): H. Kajiura-Kobayashi, T. Kobayashi, and Y. Nagahama
  • Journal Title: Dev. Dyn 232(4) Pages: 1115-1123
  • Peer Reviewed
• [Journal Article] Two types of aromatase with different encoding genes, tissue distribution and developmental Gen (2005)
  • Author(s): X. Chang, T. Kobayashi(他4人)
  • Journal Title: Comp. Endocrinol 141(2) Pages: 101-115
  • Peer Reviewed
• [Journal Article] Cloning, expression and characterization of three types of 17b-hydroxysteroid dehydrogenases from the Nile tilapia, Oreochromis niloticus (2005)
  • Author(s): L.Y. Zhou, T. Kobayashi(他6人)
  • Journal Title: J. Mol. Endocrinol 35(1) Pages: 103-116
  • Peer Reviewed
• [Journal Article] Molecular cloning, gene expression and characterization of the third estrogen receptor of the Nile tilapia, Oreochromis niloticus (2005)
  • Author(s): D.S. Wang, T. Kobayashi(他6人)
  • Journal Title: Fish Physiol. Biochem 31(2, 3) Pages: 255-266
  • Peer Reviewed
• [Journal Article] Ontogenic expression patterns of several nuclear receptors and cytochrome P450 aromatases in Brain and gonads of the Nile tilapia Oreochromis niloticus suggests their involvement in sex differentiation (2005)
  • Author(s): C.C. Sudhakumari, T. Kobayashi(他5人)
  • Journal Title: Fish Physiol. Biochem 31(2,3) Pages: 129-135
  • Peer Reviewed
• [Journal Article] Immunocytochemical localization of gonadotropins during the development of XX and XY Nile tilapia (2005)
  • Author(s): F. Sakai, T. Kobayashi(他7人)
  • Journal Title: Fish Physiol. Biochem 31(2, 3) Pages: 177-181
  • Peer Reviewed
• [Journal Article] 'Post-sex differentiational' sex reversal in the female common carp (Cyprinus carpio).
  • Author(s): Satoshi Ogawa
  • Journal Title: CYBIUM International Journal of Ichthyology, (In press)
  • Peer Reviewed
• [Presentation] ヤイトハタEpinephelus malabaricusの性分化と雄性ホルモン処理による早期性転換誘導の試み (2009)
  • Author(s): 村田良介
  • Organizer: 海産無脊椎動物研究会
  • Place of Presentation: 愛媛
  • Year and Date: 2009-03-10
• [Presentation] 雌性先熟魚ミツボシキュウセンHalichoeres tirmaculatusの性転換過程における細胞増殖 (2009)
  • Author(s): 野津了
  • Organizer: 海産無脊椎動物研究会
  • Place of Presentation: 愛媛
  • Year and Date: 2009-03-10
• [Presentation] サンゴ礁に生息する魚類の性転換 (2009)
  • Author(s): 中村將
  • Organizer: 国際シンポジウム・海生動物の繁殖
  • Place of Presentation: 沖縄
  • Year and Date: 2009-02-21
• [Presentation] サンゴ礁に生息する魚類の性転換 (2009)
  • Author(s): 中村將
  • Organizer: 国際シンポジウム・海生動物の繁殖
  • Place of Presentation: 沖縄県本部町
  • Year and Date: 2009-02-21
• [Presentation] ヤイトハタEpinephelus malabaricusの性分化と雄生ホルモン処理による早期性転換誘導の試み (2009)
  • Author(s): 村田良介
  • Organizer: 平成21年度日本水産学会春季大会
  • Place of Presentation: 東京都品川
• [Presentation] 魚類の生殖機構-基礎と応用-性転換魚類 (2009)
  • Author(s): 小林靖尚
  • Organizer: 平成21年度日本水産学会春季大会
  • Place of Presentation: 東京都品川
• [Presentation] 雌性先熟魚ミツボシキュウセンにおける性転換過程の生殖腺と体細胞の増殖 (2009)
  • Author(s): 野津了
  • Organizer: 平成21年度日本水産学会春季大会
  • Place of Presentation: 東京都品川
• [Presentation] コイ雄生殖腺の性的可塑性についての研究 成長と性分化の関連性 (2009)
  • Author(s): 小川智史
  • Organizer: 平成21年度日本水産学会春季大会
  • Place of Presentation: 東京都品川
• [Presentation] サンゴ礁魚類の性転換 生殖生物学の歴史と展望 (2008)
  • Author(s): 中村將
  • Organizer: 基礎生物学研究所研究会
  • Place of Presentation: 岡崎コンファレンスセンター
  • Year and Date: 2008-03-24
• [Presentation] 魚類の性決定・性分化とステロイドホルモン (2008)
  • Author(s): 長濱嘉孝, 中村將
  • Organizer: 日本動物学会第79大会
  • Place of Presentation: 福岡大学
• [Presentation] Endocrine and molecular control of sex change in grouper. (2008)
  • Author(s): Mohammad Ashraful Alam
  • Organizer: International Symposium on Sex Determination and Gametogenesis in Fish : Current Status and Future Directions.
  • Place of Presentation: Honolulu, Hawaii
• [Presentation] In vivo and in vitro studies of bidirectional sex change in the gonads of thehermaphrodite wrasse. (2008)
  • Author(s): Kohei Ohta
  • Organizer: International Symposium on Sex Determination and Gametogenesis in Fish : Current Status and Future Directions.
  • Place of Presentation: Honolulu, Hawaii
• [Presentation] Sex reversal of the adult gonochoristic fish nile tilapia : evidence for the existence of sexual plasticity in adult gonads. (2008)
  • Author(s): Ramji Bhandari
  • Organizer: International Symposium on Sex Determination and Gametogenesis in Fish : Current Status and Future Directions.
  • Place of Presentation: Honolulu, Hawaii
• [Presentation] Expression of estrogen receptors during sex change in the protogynous wrasse, Halichoeres trimaculatus. (2008)
  • Author(s): Ryo. Horiguchi
  • Organizer: International Symposium on Sex Determination and Gametogenesis in Fish : Current Status and Future Directions.
  • Place of Presentation: Honolulu, Hawaii
• [Presentation] Expression of forkhead transcriptional factor 2(fox12)in the protogynous wrasse, Halichoeres trimaculatus. (2008)
  • Author(s): Yasuhisa Kobayashi
  • Organizer: International Symposium on Sex Determination and Gametogenesis in Fish : Current Status and Future Directions.
  • Place of Presentation: Honolulu, Hawaii
• [Presentation] Short term treatment of aromatase inhibitor for the induction of sex change in protogynous three-spot wrasse(Halichoeres trimaculatus). (2008)
  • Author(s): Ryo Nozu
  • Organizer: International Symposium on Sex Determination and Gametogenesis in Fish : Current Status and Future Directions.
  • Place of Presentation: Honolulu, Hawaii
• [Presentation] Sexual plasticity in the ovary of common carp(Cyprinus carpio). (2008)
  • Author(s): Satoshi Ogawa
  • Organizer: International Symposium on Sex Determination and Gametogenesis in Fish : Current Status and Future Directions.
  • Place of Presentation: Honolulu, Hawaii
• [Presentation] Ovarian development and spawning with lunar periodicity in the honeycomb grouper. (2008)
  • Author(s): Kiyoshi Soyano
  • Organizer: International Symposium on Sex Determination and Gametogenesis in Fish : Current Status and Future Directions.
  • Place of Presentation: Honolulu, Hawaii
• [Presentation] A TGF-beta superfamily protein(MR-2)induces testicular differentiation as the formation of seminiferous tubule structure in Nile tilapia. (2008)
  • Author(s): Kobayashi, T
  • Organizer: International Symposium on Sex Determination and Gametogenesis in Fish : Current Status and Future Directions.
  • Place of Presentation: Honolulu, Hawaii
• [Presentation] Molecular cloning and expression of Fox12 in the protogynous wrasse, Halichoeres trimaculatus. (2008)
  • Author(s): Kobayashi Y
  • Organizer: 6th International Symposium on Fish Endocrinology.
  • Place of Presentation: Calgary, Canada
• [Presentation] 魚類の性決定・性分化とステロイドホルモン (2008)
  • Author(s): 長濱嘉孝
  • Organizer: 日本動物学会第79大会
  • Place of Presentation: 福岡大学(福岡)
• [Presentation] 精巣構成細胞の性的可塑性 (2008)
  • Author(s): 小林 亨
  • Organizer: 日本動物学会第79大会
  • Place of Presentation: 福岡大学(福岡)
• [Presentation] Gonadal maturation and lunar-related spawning in the honeycomb grouper, Epinephelus merra. (2008)
  • Author(s): Kiyoshi Soyano
  • Organizer: 5^<th> World Fisheries Congress
  • Place of Presentation: Yokohama, Japan
• [Presentation] Physiologic research on sex change in the important aquaculture fish : protandrous grouper, Epinephelus merra. (2008)
  • Author(s): Yasuhisa Kobayashi
  • Organizer: 5^<th> World Fisheries Congress
  • Place of Presentation: Yokohama, Japan
• [Presentation] Molecular character patterns during sex cization of estrogen receptor a. bl and b2 and their expressionhange in the protogynous wrasse, Halichoeres trimaculatus (2008)
  • Author(s): Ryo Horiguchi
  • Organizer: 5th International Meeting on Reproductive Biology of Aquatic Animals of the East China Sea Jeju
  • Place of Presentation: Jeju, Korea
• [Presentation] 雌性先熟魚ミツボシキュウセンにおけるFoxl2の発現変化 (2008)
  • Author(s): 小林靖尚
  • Organizer: 琉球大学熱帯生物圏研究センター平成20年度共同利用研究会
  • Place of Presentation: 沖縄県西表
• [Presentation] Somatic cell differentiation during gonadal differentiation in the teleost fish (2008)
  • Author(s): Kobayashi, T
  • Organizer: 琉球大学熱帯生物圏研究センター平成20年度共同利用研究会
  • Place of Presentation: 沖縄県西表
• [Presentation] 浮遊性をもつ造礁サンゴ卵における脂質成分の分析 (2008)
  • Author(s): 三田雅敏
  • Organizer: 琉球大学熱帯生物圏研究センター平成20年度共同利用研究会
  • Place of Presentation: 沖縄県西表
• [Presentation] 成魚雌ティラピアにおけるアロマターゼ阻害剤による性転換誘導 (2008)
  • Author(s): 小島豊
  • Organizer: 琉球大学熱帯生物圏研究センター平成20年度共同利用研究会
  • Place of Presentation: 沖縄県西表
• [Presentation] 雌性先熟魚ミツボシキュウセンDMRT1のクローニング及び性転換過程における発現解析 (2008)
  • Author(s): 堀口涼
  • Organizer: 琉球大学熱帯生物圏研究センター平成20年度共同利用研究会
  • Place of Presentation: 沖縄県西表
• [Presentation] ヤイトハタEpinephelus malabaricusの性分化機構の解明と稚魚期での早期性転換誘導の試み (2008)
  • Author(s): 村田良介
  • Organizer: 琉球大学熱帯生物圏研究センター平成20年度共同利用研究会
  • Place of Presentation: 沖縄県西表
• [Presentation] コイ雌生殖腺の性的可塑性 卵巣内における精子形成の誘導 (2008)
  • Author(s): 小川智史
  • Organizer: 第33回日本比較内分泌学会大会
  • Place of Presentation: 広島大学(広島)
• [Presentation] 沖縄の魚の性転換 (2007)
  • Author(s): 中村將
  • Organizer: 第17回日本病態生理学会大会
  • Place of Presentation: 那覇市沖縄
• [Presentation] (2007)
  • Organizer: 部会
  • Place of Presentation: 東京海洋大学
• [Presentation] Sexually dimorphic differentiation of gonadal somatic cells during sex differentiation in the teleost fish, tilapia, Oreochromis niloticus. (2007)
  • Author(s): Kobayashi, T.
  • Organizer: 8th International Symposium on Reproductive Physiology of Fish
  • Place of Presentation: Saint Malo (France)
• [Presentation] 生殖腺性分化過程における実質細胞の挙動と組織構築 (2007)
  • Author(s): 小林 亨
  • Organizer: 日本動物学会第78回大会
  • Place of Presentation: 弘前大学
• [Presentation] Post-sex differentiational sex reversal in the female common carp (Cyprinuscarpio) (2007)
  • Author(s): Satoshi Ogawa
  • Organizer: 8th International Symposium on Reproductive Physiology of Fish
  • Place of Presentation: Saint Malo (France)
• [Presentation] Changes in steroidogenic enzyme, steroidogenic acute regulatory protein, steroid receptor, gonadotropin receptor and transcriptional factor mRNAs in ovarian follicles during oocyte growth and maturation of medaka (Oryziaslatipes) (2007)
  • Author(s): Yasushi Shibata
  • Organizer: 8th International Symposium on Reproductive Physiology of Fish
  • Place of Presentation: Saint Malo (France)
• [Presentation] Quantification of FSH-and LH-receptor messenger RNA in gobiid fish (Trimma okinawae) during serial sex change. (2007)
  • Author(s): Yasuhisa Kobayashi
  • Organizer: 8th International Symposium on Reproductive Physiology of Fish
  • Place of Presentation: Saint Malo (France)
• [Presentation] The role of estrogen in gonadal sex differentiation in protandrous anemone fish Amphiprion clakii (2007)
  • Author(s): Saori Miura
  • Organizer: 8th International Symposium on Reproductive Physiology of Fish
  • Place of Presentation: Saint Malo (France)
• [Presentation] Molecular cloning and expression of three estrogen receptors in the protogynous wrasse, Halichoeres trinaculatus. (2007)
  • Author(s): Ryo Horiguchi
  • Organizer: 8th International Symposium on Reproductive Physiology of Fish
  • Place of Presentation: Saint Malo (France)
• [Presentation] Sex change in coral reef fish. (2007)
  • Author(s): Masaru Nakamura
  • Organizer: 4th Japan-Korea, korea-Japan Joint Meeting on Reproductive Biology of Aquatic Animals.
  • Place of Presentation: 長崎大学
• [Presentation] Endocrine Mechanism of Sex change in grouper. Korea-Japan (2006)
  • Author(s): M. Nakamura
  • Organizer: Japan-Korea Joint Meeting on Reproductive Biology of Aquatic Animals.Jeju,Jeju Special Self-Governing Province
  • Place of Presentation: Korea
• [Presentation] Sex change of grouer (2006)
  • Author(s): M. Nakamura
  • Organizer: International Conference of Ecophysiology in Marine Orgarisms(海洋生物生理生態)
  • Place of Presentation: 国立台湾海洋大学
• [Presentation] 魚類の性転換 (2005)
  • Author(s): 中村將
  • Organizer: 第76回大会日本動物学会
  • Place of Presentation: つくば国際会議場
• [Presentation] Sex change in fish Marine Bio-Manipulation Frontier for Food Production The 4^<th> International Symposium(Joint International Symposia of the 21^<st> COE programs of Hokkaido University, Kinki University, Ehime University and University of the Ryukyus) (2005)
  • Author(s): M. Nakamura
  • Organizer: Reproductive, Genetic and Disease Management in Aquaculture and Ocean Ranching
  • Place of Presentation: Hakodate Kokusai Hotel
• [Presentation] Sex change in fish Marine Bio-Manipulation Frontier for Food Production The 4^<th> International Symposium(Joint International Symposia of the 21^<st> COE Proceedings of Hokkaido University, Kinki University, Ehime University and University of the Ryukyus) (2005)
  • Author(s): M. Nakamura
  • Organizer: Reproductive, Genetic and Disease Management in Aquaculture and Ocean Ranching
  • Place of Presentation: Hakodate Kokusai Hotel
• [Book] 環境ホルモン-水産生物に対する影響実態と作用機構 (2006)
  • Author(s): 平井俊朗
  • Total Pages: 20
  • Publisher: 恒星社厚生閣、魚類の性分化と内分泌かく乱物質
• [Book] 美ら島の自然史サンゴ礁島嶼系の生物多様性 (2006)
  • Author(s): 小林靖尚
  • Total Pages: 16
  • Publisher: 東海大学出版会サンゴ礁魚類の性の多様性-雌から雄へ、雄から雌へ-
• [Book] 月周産卵魚カンモンハタの産卵関連行動「テレメトリー-水生動物の行動と漁具の運動解析」 (2006)
  • Author(s): 征矢野清
  • Total Pages: 9
  • Publisher: 恒星社厚生閣