| Project/Area Number |
17208029
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Boundary agriculture
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| Research Institution | Kobe University |
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Principal Investigator |
INUI Hideyuki Kobe University, Organization of Advanced Science and Technology Research Center for Environmental Genomics, Assistant Professor (90314509)
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| Co-Investigator(Kenkyū-buntansha) |
OHKAWA Hideo Fukuyama Univ, Department of Applied Biological Science, Professor (90233048)
KIKUTA Yasushi Fukuyama Univ, Department of Applied Biological Science, Associate Professor (50224895)
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| Project Period (FY) |
2005 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥48,880,000 (Direct Cost: ¥37,600,000、Indirect Cost: ¥11,280,000)
Fiscal Year 2007: ¥14,300,000 (Direct Cost: ¥11,000,000、Indirect Cost: ¥3,300,000)
Fiscal Year 2006: ¥14,300,000 (Direct Cost: ¥11,000,000、Indirect Cost: ¥3,300,000)
Fiscal Year 2005: ¥20,280,000 (Direct Cost: ¥15,600,000、Indirect Cost: ¥4,680,000)
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| Keywords | Estrogen Receptor / Aryl Hydrocarbon Receptor / Endocrine Disruptors / Dioxins / Monitoring / Transgenic Plant / Persistent Organic Pollutants / Ornamental Plant / アリルハイドロカーボン受容 / モニタリング |
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| Research Abstract |
1. Development of the Receptor-mediated Supersensitive Monitoring System of POPs
A. Supersensitive Receptors
The recombinant transcriptional factors including mouse (m), guinea pig (g), or human (h) aryl hydrocarbon receptors (XmDV, XgDV, XhDV) were constructed and introduced into tobacco and Arabidopsis plants together with the β-glucuronidase (GUS) reporter gene. When the transgenic plants were cultured with polychlorinated biphenyls (PCBs), induction of the GUS activity was dependent on PCB toxicity. The XmDV transgenic tobacco plants successfully detected dioxin contamination in soil.
Transgenic Arabidopsis plants harboring the recombinant medaka estrogen receptor (ER) were obtained.
B. POPs. Absorption, Metabolism, and Accumulation in Plants
The zucchini cultivars that absorbed and accumulated 180 times more dioxins than tobacco plants were identified. We demonstrated that desorption of dioxins from soil matrices, absorption from surface of roots into cells, and translocation to aerial
… More
parts were important for dioxin absorption in the zucchini, and that citric acid was responsible for high accumulation of dioxins.
C. Signal Transduction System for POPs Contamination
Novel recombinant transcriptional factors including movement protein (MP) that was involved in intercellular transporting of viruses in plant cells were introduced into the XgDV transgenic Arabidopsis plants. The GUS activity in the flower stalks of the MP/XgDV transgenic plants treated with PCB126 significantly increased, therefore the dioxin contamination signal was successfully transmitted to the aerial parts of the plants.
2. Development of Transgenic Ornamental Plants for Monitoring of POPs
A. Transformation of Petunia, Torenia, and Verbena
Transgenic ornamental plants harboring the XmDV, XgDV, or ER, and RNAi of flower color synthesis genes were obtained.
B. Monitoring of Dioxin Contaminated Soil Using the Transgenic Ornamental Plants
A part of the flower color changed in the transgenic ornamental plants that were cultivated in the dioxin contaminated soil, but more research needs to be done for stable monitoring. Less
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