Molecular mechanism of functions of photosynthetic CO_2-fixing enzyme RuBisCO

• Project/Area Number: 17208031
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied molecular and cellular biology
• Research Institution: Nara Institute of Science and Technology
• Principal Investigator: YOKOTA Akiho Nara Institute of Science and Technology, バイオサイエンス研究科, 教授 (40118005)
• Co-Investigator(Kenkyū-buntansha): AKASHI Kinya 奈良先端科学気出大学院大学, バイオサイエンス研究科, 助教 (20314544) ; ASHIDA Hiroki 奈良先端科学気出大学院大学, バイオサイエンス研究科, 助教 (50362851) ; MUNEKAGE Yuri 奈良先端科学気出大学院大学, バイオサイエンス研究科, 助教 (30423247)
• Project Period (FY): 2005 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥47,970,000 (Direct Cost: ¥36,900,000、Indirect Cost: ¥11,070,000); Fiscal Year 2008: ¥9,360,000 (Direct Cost: ¥7,200,000、Indirect Cost: ¥2,160,000); Fiscal Year 2007: ¥13,520,000 (Direct Cost: ¥10,400,000、Indirect Cost: ¥3,120,000); Fiscal Year 2006: ¥12,740,000 (Direct Cost: ¥9,800,000、Indirect Cost: ¥2,940,000); Fiscal Year 2005: ¥12,350,000 (Direct Cost: ¥9,500,000、Indirect Cost: ¥2,850,000)
• Keywords: 光合成 / 炭素固定 / ルビスコ / 機能分子進化 / 機能改良 / カルビン回路 / 古細菌 / ルビスコ祖先蛋白質 / ホスホリブロキナーゼ / ウリジンキナーゼ / ガルディエリア / ルビスコ生合成 / ルビスコ祖先タンパク質 / 分子進化 / タンパク質合成 / ホスホリプロキナーゼ

Research Abstract

ルビスコの反応触媒の分子機構およびルビスコ生合成の制御機構の解明を行ってきた.ルビスコ反応触媒の分子機構の解明を目指し、すでに枯草菌に発見しているルビスコ活性を持たないルビスコ祖先蛋白質とルビスコの反応中心残基の触媒反応への関わり方を明らかにした.我々が発見したもっとも高いSrel値を持つ紅藻Galdieriaルビスコと植物ルビスコの蛋白質構造比較から、オキシゲナーゼ反応抑制残基と想定している残基をラン藻ルビスコへ導入し、その機能を明らかにした.ルビスコ生合成の制御機構に関しては、ルビスコを正常に合成できない変異株を多数スクリーニングし、分子遺伝学手法によってそれらの変異原因遺伝子を特定し、その機能解析からルビスコ生合成の制御機構を明らかにした.

Research Products

• [Journal Article] pfkB-type carbohydrate kinase family protein, NARA5, for massive expressions of plastid-encoded photosynthetic genes in Arabidopsis thaliana (2009)
  • Author(s): Taro Ogawa, Kenji Nishimura, Takehiko Aoki, Hisabumi Takase, Ken-Ichi Tomizawa, Hiroki Ashida and Akiho Yokota
  • Journal Title: Plant Physiol. [Epub ahead of print]
  • Peer Reviewed
• [Journal Article] Structure of the apo decarbamylated form of 2, 3-diketo-5-methylthiopentyl-1-phosphate enolase from Bacillus subtilis (2009)
  • Author(s): Haruka Tamura, Yohtaro Saito, Hiroki Ashida, Yasushi, Kai, Tsuyoshi Inoue, Akiho Yokota and Hiroyoshi Matsumura
  • Journal Title: Acta Crystallograph Sec.D.65 Pages: 942-951
  • Peer Reviewed
• [Journal Article] The long-term responses of the photosynthetic proton circuit to drought (2009)
  • Author(s): Kaori Kohzuma, Jeff A. Cruz, Kinya Akashi, Saki Hoshiyasu, Yuri Nakajima-Munekage, Akiho Yokota and David M. Kramer
  • Journal Title: Plant Cell Environm 32 Pages: 209-219
  • Peer Reviewed
• [Journal Article] Structural and functional similarities between a RuBisCO-like protein from Bacillus subtilis and photosynthetic RuBisCO. (2009)
  • Author(s): Yohtaro Saito, Hiroki Ashida, Tomoko Sakiyama, Nicole Tandeau de Marsac, Antoine Danchin, Agnieszka Sekowska, and Akiho Yokota
  • Journal Title: J. Biol. Chem. 284 Pages: 13256-13264
  • Peer Reviewed
• [Journal Article] Crystallization and preliminary X-ray analysis of 2, 3-diketo-5-methylthiopentyl-1-phosphate enolase from Bacillus subtilis (2009)
  • Author(s): Haruka Tamura, Hiroki Ashida, Shogo Koga, Yohtaro Saito, Tomonori Yadani, Yasushi Kai, Tsuyoshi Inoue, Akiho Yokota and Hiroyoshi Matsumura
  • Journal Title: Acta Crystallographica Section 65 Pages: 147-50
  • Peer Reviewed
• [Journal Article] Structural and functional similarities between a RuBisCO-like protein from Bacillus subtilis and photosynthetic RuBisCO. (2009)
  • Author(s): Yohtaro Saito
  • Journal Title: Journal of Biological Chemistry (in press)
  • Peer Reviewed
• [Journal Article] Crystallization and preliminary x-ray analysis of 2, 3-diketo-5-methylthiopentyl-1-phosphate enolase from Bacillus subtilis. (2009)
  • Author(s): Haruka Tamura
  • Journal Title: Acta Crystallographica Section F 65 Pages: 147-150
  • Peer Reviewed
• [Journal Article] RuBisCO-like proteins as the enolase enzyme in the methionine salvage pathway : Functional and evolutionary relationships between RuBisCO-like proteins and photosynthetic RuBisCO. (2008)
  • Author(s): Hiroki Ashida, Yohtaro Saito, Toshihiro Nakano, Nicole Tandeau de Marsac, Agnieszka Sekowska, Antoine Danchin and Akiho Yokota
  • Journal Title: J. Exp. Bot. 59 Pages: 1543-54
  • Peer Reviewed
• [Journal Article] Molecular mechanisms of RuBisCO biosynthesis in higher plants (2008)
  • Author(s): Kenji Nishimura, Taro Ogawa, Hiroki Ashida, and Akiho Yokota
  • Journal Title: Plant Biotech 25 Pages: 285-290
  • NAID: 10021913713
• [Journal Article] Enzymatic characterization of 5-methylthioribulose-1-phosphate dehydratase of the methionine salvage pathway from Bacillus subtilis. (2008)
  • Author(s): Hiroki Ashida, Yohtaro Saito, Chojiro Kojima and Akiho Yokota
  • Journal Title: Biosci Biotechnol Biochem 72 Pages: 959-967
  • Peer Reviewed
• [Journal Article] RuBisCO-like proteins as the enolase enzyme in the methionine salvage pathway : Functional and evolutionary relationships between RuBisCO-like proteins and photosynthetic RuBisCO. (2008)
  • Author(s): Hiroki Ashida
  • Journal Title: Journal of Experimental Botany 59 Pages: 1543-1554
  • Peer Reviewed
• [Journal Article] Molecular mechanisms of RuBisCO biosynthesis in higher plants. (2008)
  • Author(s): Kenji Nishimura
  • Journal Title: Plant Biotechnology 25 Pages: 285-290
• [Journal Article] Enzymatic characterization of 5-methylthioribulose-1-phosphate dehydratase of the methionine salvage pathway from Bacillus subtilis. (2008)
  • Author(s): Hiroki Ashida
  • Journal Title: Bioscience, Biotechnology, and Biochemistry 72 Pages: 959-967
  • Peer Reviewed
• [Journal Article] RuBisCO-like proteins as the enolase enzyme in the methionine salvage pathway: Functional and evolutionary relationships between RuBisCO-like proteins and photosynthetic RuBisCO. (2008)
  • Author(s): Hiroki Ashida
  • Journal Title: Journal of Experimental Botany 59 Pages: 1543-1554
  • Peer Reviewed
• [Journal Article] Molecular mechanisms of RuBisCO biosynthesis in higher plants. (2008)
  • Author(s): Kenji Nishimura
  • Journal Title: Plant Biotechnology 25(in press)
  • Peer Reviewed
• [Journal Article] Enzymatic characterization of 5-methylthioribulose-1-phosphate dehydratase of the methionine salvage pathway from Bacillus subtilis. (2008)
  • Author(s): Hiroki Ashida
  • Journal Title: Bioscience Biotechnology Biochemistry 72 Pages: 959-967
  • Peer Reviewed
• [Journal Article] Crystal structure of 5-methylthioribose 1-phosphate isomerase product complex from Bacillus subtilis : Implications for catalytic mechanism. (2007)
  • Author(s): Haruka Tamura, Yohtaro Saito, Hiroki Ashida, Tsuyoshi Inoue, Yasushi Kai, Akiho Yokota, and Hiroyoshi Matsumura
  • Journal Title: Protein Sci. 17 Pages: 126-35
  • Peer Reviewed
• [Journal Article] Enzymatic Characterization of 5-Methylthioribose 1-Phosphate Isomerase from Bacillus subtilis (2007)
  • Author(s): Yohtaro Saito, Hiroki Ashida, Chojiro Kojima, Haruka Tamura, Hiroyoshi Matsumura, Yasushi Kai, Akiho Yokota
  • Journal Title: Biosci Biotechnol Biochem 71 Pages: 2021-8
  • NAID: 10027517927
  • Peer Reviewed
• [Journal Article] Crystal structure of 5-methylthioribose 1-phosphate isomerase product complex from Bacillus subtilis: Implicationsfor catalytic mechanism. (2007)
  • Author(s): Haruka Tamura
  • Journal Title: Protein Science 17 Pages: 126-135
  • Peer Reviewed
• [Journal Article] Enzymatic Characterization of 5-Methylthioribose 1-Phosphate Isomerase from Bacillus subtilis. (2007)
  • Author(s): Yohtaro Saito
  • Journal Title: Bioscience Biotechnology Biochemistry 71 Pages: 2021-2028
  • Peer Reviewed
• [Journal Article] Isabelle Callebaut, Agnieszka Sekowska, Elke Dittmann, Christiane Bouchier, and Nicole Tandeau de Marsac A New Rubisco-like Protein Coexists with a Photosynthetic Rubisco in the Planktonic Cyanobacteria Microcystis. (2006)
  • Author(s): Alyssa Carre-Mlouka, Annick Mejean, Philippe Quillardet, Hiroki Ashida, Yohtaro Saito, Akiho Yokota
  • Journal Title: J. Biol. Chem. 281 Pages: 24462-24471
  • Peer Reviewed
• [Journal Article] A new Rubisco-like protein coexists with a photosynthetic Rubisco in the planktonic cyanobacteria Microcystis (2006)
  • Author(s): Carre-Mlouka, A., Quillardet, P., Ashida, H., Saito, Y., Yokota, A., Callebaut, I., Sekowska, A., Dittmann, E., Bouchier, C., Marsac, N.T.
  • Journal Title: Journal of Biological Chemistry 281・34 Pages: 24462-24471
• [Journal Article] A Was photosynthetic RuBisCO recruited by acquisitive evolution from RuBisCO-like proteins involved in sulfur metabolism? (2005)
  • Author(s): Ashida, H., Danchin, A., Yokota, A.
  • Journal Title: Research in Microbiology 156 Pages: 611-618
• [Journal Article] Crystallization and preliminary X-ray analysis of methylthioribose-1 -phosphate isomerase from Bacillus subtilis (2005)
  • Author(s): Tamura, H., Matsumura, H., Inoue, T., Ashida, H., Saito, Y., Yokota, A.and Kai, Y.
  • Journal Title: Acta Crystallographica Section F 61 Pages: 595-598
• [Presentation] シロイヌナズナのpfkB-type carbohydrate kinase family protein, NARA5はプラスチドゲノムにコードされる光合成遺伝子の高発現に必須である (2009)
  • Author(s): 小川太郎
  • Organizer: 第50回日本植物生理学会年会 創立50周年記念大会
  • Place of Presentation: 名古屋大学
  • Year and Date: 2009-03-21
• [Presentation] 葉緑体に局在するシロイヌナズナDEAD-box RNA helicase RH39の機能解析 (2009)
  • Author(s): 西村健司
  • Organizer: 第50回日本植物生理学会年会 創立50周年記念大会
  • Place of Presentation: 名古屋大学
  • Year and Date: 2009-03-21
• [Presentation] pfkB-type carbohydrate kinase family protein, NARA5, is essential for the massive expressions of plastid-encoded photosynthetic genes in Arabidopsis thaliana. (2009)
  • Author(s): Taro Ogawa
  • Organizer: Nissan Science Foundation, Woody Plants Biotechnology Symposum
  • Place of Presentation: Scieum, Tokyo
  • Year and Date: 2009-02-26
• [Presentation] A DEAD-box RNA helicase RH39 is Required for the Biogenesis of Cmoroplast 50S Ribosomes and Essential for Plant Development in Arabidopsis thaliana. (2009)
  • Author(s): Kenji Nishimura
  • Organizer: Nissan Science Foundation, Woody Plants Biotechnology Symposium
  • Place of Presentation: Science Hall in the Science Museum, Tokyo
  • Year and Date: 2009-02-26
• [Presentation] シロイヌナズナ葉緑体に局在するDEAD-box RNA helicase RH39の機能解析 (2009)
  • Author(s): 西村健司
  • Organizer: 日本農芸化学会 関西支部例会(第458回講演会)
  • Place of Presentation: 京都
  • Year and Date: 2009-02-07
• [Presentation] メチオニン再生経路の酵素群によるリボース新規代謝経路 (2008)
  • Author(s): 中野寿宏、蘆田弘樹、齋藤洋太郎、横田明穂
  • Organizer: 日本農芸化学会2008 年度大会
  • Place of Presentation: 名城大学天白キャンパス
  • Year and Date: 2008-03-27
• [Presentation] ラン藻カルボキシソーム構成タンパク質CcmMが有するRuBisCO small subunit-like domainとRuBisCO small subunitの機能的関連性 (2008)
  • Author(s): 山下 宣容
  • Organizer: 日本農芸化学会年会
  • Place of Presentation: 名城大学天白キャンパス
  • Year and Date: 2008-03-27
• [Presentation] メチオニン再生経路の酵素群によるリボース新規代謝経路 (2008)
  • Author(s): 中野寿宏
  • Organizer: 日本農芸化学会年会
  • Place of Presentation: 名城大学天白キャンパス
  • Year and Date: 2008-03-27
• [Presentation] RuBisCO-like proteinの解析から見えてきたRuBisCO誕生の分子機構 (2008)
  • Author(s): 蘆田弘樹、横田明穗
  • Organizer: 日本植物生理学会第49回年会シンポジウム植物の生産性とカルビン回路「カルビンサイクル研究の新展開」
  • Place of Presentation: ゲストスピーカー
  • Year and Date: 2008-03-22
• [Presentation] RuBisCO-like proteinの解析から見えてきたRuBisCO誕生の分子機構 (2008)
  • Author(s): 蘆田弘樹
  • Organizer: 日本植物生理学会第49回年会シンポジウム
  • Place of Presentation: 札幌コンベンションセンター
  • Year and Date: 2008-03-22
• [Presentation] Analysis of the molecular evolution of Phosphoribulokinase, a key Calvin cycle enzyme, in primitive cyanobacterium Gloeobacter violaceous. (2008)
  • Author(s): SANDHYA MEHROTRA
  • Organizer: 日本植物生理学会年会
  • Place of Presentation: 札幌コンベンションセンター
  • Year and Date: 2008-03-21
• [Presentation] 枯草菌RuBisCO-like proteinと光合成RuBisCOに共通する構造と機能 (2008)
  • Author(s): 齋藤洋太郎、蘆田弘樹、Agnieszka Sekowska、Antoine Danchin、横田明穗
  • Organizer: 第49回日本植物生理学会年会(奈良先端大・バイオ, パスツール研)
  • Place of Presentation: 札幌
  • Year and Date: 2008-03-20
• [Presentation] シロイヌナズナ葉緑体rRNAの成熟化に異常があるnara12変異株の解析 (2008)
  • Author(s): 西村健司
  • Organizer: 日本植物生理学会年会
  • Place of Presentation: 札幌コンベンションセンター
  • Year and Date: 2008-03-20
• [Presentation] 枯草菌RuBisCO-like proteinと光合成RuBisCOに共通する構造と機能 (2008)
  • Author(s): 齋藤洋太郎
  • Organizer: 日本植物生理学会年会
  • Place of Presentation: 札幌コンベンションセンター
  • Year and Date: 2008-03-20
• [Presentation] RuBisCO-like proteinの研究から光合成CO_2固定酵素RuBisCOの分子進化の謎にせまる (2008)
  • Author(s): 蘆田弘樹
  • Organizer: 分子研研究会「分子の視点から見る光合成」招待講演
  • Place of Presentation: 岡崎コンファレンスセンター
  • Year and Date: 2008-03-11
• [Presentation] Improvement of Cyanobacterial Ribulose 1, 5-Bisphosphate Carboxylase/Oxygenase by Introducing the Latch Structure Involved in CO2/O2 Specificity in red algal RuBisCO (2008)
  • Author(s): Yohtaro Saito, Nana Ninomiya, Hiroki Ashida, and Akiho Yokota
  • Organizer: The 4^<th> INDONESIAN BIOTECHNOLOGY CONFERENCE
  • Place of Presentation: Nara Institute of Science and Technology (NAIST), Graduate School of Biological Sciences, 8916-5 Takayama, Ikoma, Nara 630-0101, Japan
• [Presentation] Analysis of common conserved residues between Bacillus RuBisCO-like protein and photosynthetic RuBisCO (2008)
  • Author(s): Toshihiro Nakano, Yohtaro Saito, Hiroki Ashida and Akiho Yokota
  • Organizer: The 4^<th> INDONESIAN BIOTECHNOLOGY CONFERENCE
  • Place of Presentation: Nara Institute of Science and Technology (NAIST), Graduate School of Biological Sciences, 8916-5 Takayama, Ikoma, Nara 630-0101, Japan
• [Presentation] Improvement of Cyanobacterial Ribulose 1, 5-Bisphosphate Carboxylasa/Oxygenase by Introducing the Latch Structure Involved in CO_2/O_2 Specificity in red algal RuBisCO. (2008)
  • Author(s): Yohtaro Saito
  • Organizer: The 4^<th> INDONESIAN BIOTECHNOLOGY CONFERENCE
  • Place of Presentation: Bogor
• [Presentation] Analysis of common conserved residues between Bacillus RuBisCO-like protein and photosynthetic RuBisCO. (2008)
  • Author(s): Toshihiro Nakano
  • Organizer: The 4^<th> INDONESIAN BIOTECHNOLOGY CONFERENCE
  • Place of Presentation: Bogor
• [Presentation] 植物葉緑体形質転換とその応用性 (2007)
  • Author(s): 蘆田 弘樹
  • Organizer: ファイトテクノロジー交流会
  • Place of Presentation: 大阪
  • Year and Date: 2007-10-20
• [Presentation] Isolation and characterization of genes necessary for achievement of RuBisCO accumulation in Arabidopsis thaliana. (2007)
  • Author(s): Kenji NISHIMURA
  • Organizer: JSPS Colloquium "Frontiers in Plant Biotechnology"
  • Place of Presentation: Stockholm University, Sweden
  • Year and Date: 2007-10-04
• [Presentation] 高CO_2親和性を示す紅藻RuBisCOが有するラッチ構造のラン藻RuBisCOへの導入 (2007)
  • Author(s): 二宮奈々、蘆田弘樹、横田明穗
  • Organizer: 2007年度松山年会(奈良先端大・バイオ)
  • Place of Presentation: 愛媛大学城北キャンパス
  • Year and Date: 2007-03-28
• [Presentation] 枯草菌RuBisCO-like protein とRuBisCOの相互構造活性相関 (2007)
  • Author(s): 中野寿宏、齋藤洋太郎、蘆田弘樹、横田明穂
  • Organizer: 日本農芸化学会2007年度大会
  • Place of Presentation: 東京農業大学世田谷キャンパス
  • Year and Date: 2007-03-26
• [Presentation] (阪大院工、奈良先端大バイオ、福井工大)メチオニン回収経路で機能する枯草菌由来RLP のX 線結晶構造解析 (2007)
  • Author(s): 田村はるか、蘆田弘樹、斉藤洋太郎、井上豪、横田明穂、甲斐泰、松村浩由
  • Organizer: 日本結晶学会2007年度年会および総会
  • Place of Presentation: 東京
• [Presentation] (阪大院工・奈良先端大院分子生物学専攻・東北大院農)イネ由来RuBisCOと紅藻由来RuBisCOの硫酸イオン複合体に見られた立体構造の違い (2007)
  • Author(s): 小村泰浩、松村浩由、蘆田弘樹、石田宏幸、上野剛、溝端栄一、井上豪、牧野周、横田明穂、前忠彦、甲斐泰
  • Organizer: 日本結晶学会2007年度年会および総会
  • Place of Presentation: 東京
• [Presentation] (Department of Applied Chemistry, Osaka University ; Department of Molecular biology, Nara Institute of Science and Technology (NAIST) ; CREST (Sosho Project), JST)Crystal structure of 5-methylthioribose 1-phosphate isomerase product complex from Bacillus subtilis : Implications for catalytic mechanism (2007)
  • Author(s): Haruka Tamura, Yohtaro Saito, Hiroki Ashida, Tsuyoshi Inoue, Yasushi Kai, Akiho Yokota, and Hiroyoshi Matsumura
  • Organizer: 第8回アジア結晶学会連合会議
  • Place of Presentation: 台北
• [Presentation] (Department of Applied Chemistry, Osaka University ; Department of Molecular biology, Nara Institute of Science and Technology (NAIST) ; Department of Applied Plant Science ; Tohoku University, CREST (Sosho Project), JST)STRUCTURAL DIFFERENCE BETWEEN RICE AND RED ALGA RUBISCO COMPLEXED WITH SULFATE (2007)
  • Author(s): Yasuhiro Komura, Hiroyoshi Matsumura, Hiroyuki Ishida, Hiroki Ashida, EiichiMizohata, Tsuyoshi Inoue, Amane Makino, Tadahiko Mae, Akiho Yokota, Yasushi Kai
  • Organizer: 第8回アジア結晶学会連合会議(The 8th Conference of the Asian Crystallographic Association, AsCA'07)
  • Place of Presentation: 台北
• [Presentation] Evolutionary potential of RuBisCO-like protein in Bacillus subtilis : Interaction with transition-state analogue of RuBisCO (2007)
  • Author(s): Yohtaro SAITO, Hiroki ASHIDA, Agnieszka SEKOWSKA, DANCHIN, and Akiho YOKOTA
  • Organizer: 14^<th> International congress of photosynthesis(poster)
  • Place of Presentation: Glasgow, UK
• [Presentation] Improvement of cyanobacterial RuBisCO by introducing the latch structure involved in high affinity for CO_2 in red algal RuBisCO (2007)
  • Author(s): Nana NINOMIYA, Hiroki ASHIDA, andAkiho YOKOTA
  • Organizer: 14^<th> International congress of photosynthesis(poster)
  • Place of Presentation: Glasgow, UK
• [Presentation] RuBisCO-like proteins, Research Frontiers with Rubisco - the "Elixir of Life" in the Biosphere. (2007)
  • Author(s): Hiroki ASHIDA and Akiho YOKOTA
  • Organizer: 14^<th> International Congress of Photosynthesis satellite Meeting, (guest speaker)
  • Place of Presentation: Rothamsted Research, Harpenden, Herts, UK
• [Presentation] イネ由来RuBisCOと紅藻由来RuBisCOの硫酸イオン複合体に見られた立体構造の違い (2007)
  • Author(s): 小村泰浩
  • Organizer: 日本結晶学会2007年度年会
  • Place of Presentation: 東京
• [Presentation] メチオニン回収経路で機能する枯草菌由来RLPのX線結晶構造解析 (2007)
  • Author(s): 田村はるか
  • Organizer: 日本結晶学会2007年度年会
  • Place of Presentation: 東京
• [Presentation] STRUCTURAL DIFFERENCE BETWEEN RICE AND RED ALGA RUBISCO COMPLEXED WITH SULFATE. (2007)
  • Author(s): Yasuhiro Komura
  • Organizer: The 8th Conference of the Asian Crystallographic Association, AsCA'07
  • Place of Presentation: 台北
• [Presentation] Crystal structure of 5-methylthioribose 1-phosphate isomerase product complex from Bacillus subtilis: Implications for catalytic mechanism. (2007)
  • Author(s): Haruka Tamura
  • Organizer: The 8th Conference of the Asian Crystallographic Association, AsCA'07
  • Place of Presentation: 台北
• [Presentation] Isolation and characterization of genes necessary for achievement of RuBisCO accumulation in Arabidopsis thaliana. (2007)
  • Author(s): Taro Ogawa
  • Organizer: 14th International congress of photosynthesis
  • Place of Presentation: Glasgow, UK
• [Presentation] Improvement of cyanobacterial RuBisCO by introducing the latch structure involved in high affinity for CO_2 in red algal RuBisCO. (2007)
  • Author(s): Nana NINOMIYA
  • Organizer: 14th International congress of photosynthesis
  • Place of Presentation: Glasgow, UK
• [Presentation] Evolutionary potential of RuBisCO-like protein in Bacillus subtilis: Interaction with transition-state analogue of RuBisCO. (2007)
  • Author(s): Yohtaro SAITO
  • Organizer: 14th International congress of photosynthesis
  • Place of Presentation: Glasgow, UK
• [Presentation] RuBisCO-like proteins、 guest speaker (2007)
  • Author(s): Hiroki ASHIDA
  • Organizer: Research Frontiers with Rubisco - the "Elixir of Life" in the Biosphere. l4th International Congress of Photosynthesis Satellite Meeting
  • Place of Presentation: Harpenden, UK
• [Presentation] X-ray structure of methylthioribose-1-phosphate isomerase from Bacillus subtilis reveals a hydride transfer mechanism of catalysis and a structure function relationship with other homologues (2006)
  • Author(s): Haruka TAMURA, Hiroyoshi Matsumura, Yohtaro SAITO, Tsuyoshi INOUE, HirokiASHIDA, Akiho YOKOTA, and Yasushi KAI
  • Organizer: 20th IUBMB International Congress of Biochemistry and Molecular Biology and 11th FAOBMB Congress
  • Place of Presentation: Kyoto, Japan
  • Year and Date: 2006-06-22
• [Presentation] Structure-function relationship between RuBisCO-like protein of Bacillus subtilis and photosynthetic RuBisCO (2006)
  • Author(s): Yohtaro SAITO, Agnieszka SEKOWSKA, Hiroki ASHIDA, Antoine DANCHIN, andAkiho YOKOTA
  • Organizer: 20th IUBMB International Congress of Biochemistry and Molecular Biology and 11th FAOBMB Congress
  • Place of Presentation: Kyoto, Japan
  • Year and Date: 2006-05-19
• [Presentation] Nicole Tandeau de Marsac and Akiho Yokota, Molecular evolution of RuBisCO-like proteins (2006)
  • Author(s): Hiroki Ashida, Tomoko Sakiyama, YohtaroSaito
  • Organizer: 20th IUBMB International Congress of Biochemistry and Molecular Biology and 11th FAOBMB Congress((poster))
  • Place of Presentation: Kyoto, Japan
  • Year and Date: 2006-05-19
• [Presentation] 光合成カルビン回路完成の分子的基盤 (2006)
  • Author(s): 蘆田弘樹、横田明穗
  • Organizer: 日本光合成研究会第6回ワークショップセミナーNew perspective of photosynthesis research光合成研究の新たな潮流構造とゲノムそして未来(奈良先端大、バイオサイエンス)
  • Place of Presentation: 大阪大学ゲストスピーカー
• [Presentation] ゲノムから見たカルビン回路完成の分子機構 (2006)
  • Author(s): 蘆田弘樹、横田明穂
  • Organizer: 日本植物学会第70回大会、シンポジウムゲノムから見た光合成機能の再発見(奈良先端大、バイオサイエンス)
  • Place of Presentation: 熊本ゲストスピーカー
• [Book] Engineering photosynthetic pathways, In Bioengineering and Molecular Biology of Plant Pathways, Volume 1(Eds., H. J. Bohnert and H. T. Nguyen)in Advances in Plant Biochemistry and Molecular Biology(Elsevier, Executive Editor, N. G. Lewis) (2008)
  • Author(s): Akiho Yokota
  • Total Pages: 25
  • Publisher: Elsevier
• [Book] 光、栄養環境強光乾燥ストレスに対する植物の多次元的応答 蛋白質核酸酵素増刊(Vol.52, No.6) (2007)
  • Author(s): 明石欣也、宗景ゆり、高原健太郎、横田明穂
  • Publisher: 植物における環境とあ生物ストレスに対する応答
• [Book] 第2章エネルギーと代謝第4節光合成CO_2固定酵素RuBisCO (2006)
  • Author(s): 蘆田弘樹、横田明穗
  • Publisher: プラントミメティックス~植物に学ぶ~
• [Book] プラントミメティクス〜植物に学ぶ〜光合成CO_2固定酵素RuBisCO (2006)
  • Author(s): 蘆田弘樹, 横田明穂
  • Total Pages: 8
  • Publisher: 株式会社 エヌ・ティー・エス
• [Patent(Industrial Property Rights)] 変異型のリブロース-1, 5-ビスリン酸カルボキシラーゼ/オキシゲナーゼおよびその利用 (2007)
  • Inventor(s): 蘆田弘樹、横田明穂
  • Industrial Property Rights Holder: 安田國雄
  • Filing Date: 2007-02-07
• [Patent(Industrial Property Rights)] 変異型のリブロースー1,5-ビスリン酸カルボキシラーゼ/オキシゲナーゼおよびその利用 (2007)
  • Inventor(s): 蘆田弘樹, 横田明穂
  • Industrial Property Rights Holder: 奈良先端科学技術大学院大学長
  • Industrial Property Number: 2007-028608
  • Filing Date: 2007-02-07