Stem cell regulation towards cell therapy for diabetes
| Project/Area Number |
17209043
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General surgery
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| Research Institution | Yokohama City University |
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Principal Investigator |
TANIGUCHI Hideki Yokohama City University, Graduate School of Medicine Department of Regenerative Medicine, Prof (70292555)
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| Project Period (FY) |
2005 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥49,530,000 (Direct Cost: ¥38,100,000、Indirect Cost: ¥11,430,000)
Fiscal Year 2007: ¥14,430,000 (Direct Cost: ¥11,100,000、Indirect Cost: ¥3,330,000)
Fiscal Year 2006: ¥14,430,000 (Direct Cost: ¥11,100,000、Indirect Cost: ¥3,330,000)
Fiscal Year 2005: ¥20,670,000 (Direct Cost: ¥15,900,000、Indirect Cost: ¥4,770,000)
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| Keywords | Stem Cell / Diabetes / Pancreatic islet / Regeneration |
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| Research Abstract |
Islet transplantation has become available across the globe since a novel protocol was reported. However, because donors are in short supply, only a minority of patients benefit from this procedure. Pancreatic progenitor cells are a promising resource for regeneration of new islets but if progenitor cells reside in ductal epithelium is not clear. Mouse pancreas was examined by immunohistochemistry with cell surface markers specific for ductal cells. We developed an isolation method for ductal cells by flow cytometric cell sorting using a newly identified specific marker for ductal cells. Using an in vitro colony assay, we characterized their proliferative and multipotent capacity. CD133 is specifically expressed in ductal epithelium. Flow cytometric analysis revealed that purified ductal cells are highly enriched in the CD133^+CD34^-CD45^-Ter119^- fraction. Analysis of clonal epithelial colonies formed by individual cells revealed that progenitor cells with multilineage differentiation capacity are present in neonatal ductal epithelium. Moreover, these progenitor cells express c-Met. In adult mice, progenitor cells that exhibit a high proliferative capacity but appear committed to a ductal lineage are co-purified with CD133^+CD34^-CD45^-Ter119^-cells. We established a system for isolating and culturing mouse pancreatic ductal cells that relies on flow cytometric cell sorting. Clonal analysis revealed that a population of progenitor cells is present amongst CD133^+ ductal cells. Isolation of these cells will facilitate future studies into the roles of pancreatic progenitor cells in regeneration and carcinogenesis.
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Report
(4 results)
Research Products
(68 results)
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[Journal Article] Tbx3 controls the fate of hepatic progenitor cells in liver development by suppressing p19ARF expression2008
Author(s)
Suzuki, A., Sekiya, S., Buscher, D., Izpisua, Belmonte, JC, Taniguchi, H
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Journal Title
Development 135(9)
Pages: 1589-1595
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Journal Article] Enhanced self-renewal capability in hepatic stem/progenitor cells drives cancer initiation2007
Author(s)
Chiba T, Zhen YW, Kita K, Yokosuka O, Saisho H, Onodera M, Miyoshi H, Nakano M, Zen Y, Nakanuma Y, Nakauchi H, Iwama A, Taniguchi H
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Journal Title
Gastroenterology 133
Pages: 937-950
Description
「研究成果報告書概要(和文)」より
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Peer Reviewed
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[Journal Article] Enhanced self-renewal capability in hepatic stem/progenitor cells drives cancer initiation2007
Author(s)
Chiba, T., Zhen, Y.W., Kita, K., Yokosuka, O., Saisho, H., Onodera, M., Miyoshi, H., Nakano, M., Zen, Y., Nakanuma, Y., Nakauchi, H., Iwama, A., Taniguchi, H
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Journal Title
Gastroenterology 133(3)
Pages: 937-950
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Adult rat hepatic bipotent progenitor cels remain doramant even after extensive hepatectomy2007
Author(s)
Yamamoto, Y., Togo, S., Yun-wen, Zheng, Kubota, T., Taniguchi, H., Shimada, S
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Journal Title
Wound Repair and Regeneration 15(3)
Pages: 422-429
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Neonatal pahcreatic cells redifferentiate into both neural and Communications2007
Author(s)
Zhao, W., Hirose, T., Ishikawa, M., Oshima, Y., Hirai, S., Ohno, S., Taniguchi, H
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Journal Title
Biochemical and Biophysical Research 352(1)
Pages: 84-90
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Isolation of mouse pancreatic ductal progenitor cells expressing CD133 and c-Met by flow cytometric cell sorting2007
Author(s)
Oshima, Y., Suzuki, A., Kawashimo, K., Ishikawa, M., Ohkohchi, N., Taniguchi, H
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Journal Title
Gastroenterology 132(2)
Pages: 720-732
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Production of Functional Spermatids from Mouse Germline Stem Cells in Ectopically Reconstituted Seminiferous Tubules2007
Author(s)
Kita, K., Watanabe, T., Ohsaka, K., Hayashi, H., Kubota, Y., Nagashima, Y., Aoki, I., Taniguchi, H., Noce, T., Inoue, K., Miki, H., Ogonuki, N., Tanaka, H., Ogura, A., Ogawa, T
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Journal Title
Biol Reprod 76(2)
Pages: 211-7
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Enhanced self-renewal capability in hepatic stem/progenitor cells drives cancer initiation2007
Author(s)
Chiba T, Zhen YW, Kita K, Yokosuka O, Saisho H, Onodera M, Miyoshi H, Nakano M, Zen Y, Nakanuma Y, Nakauchi H, Iwama A, Taniuchi H
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Journal Title
Gastroenterology 133
Pages: 937-950
Related Report
Peer Reviewed
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[Journal Article] Side Population Purified From Hepatocellular Carcinoma Cells Harbors Cancer Stem Cell- like Properties2006
Author(s)
Chiba, T., Kita, K., Zhen, Y.W., Yokosuka, O., Saisho, H., Iwama, A., Nakauchi, H., Tanisuchi, H
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Journal Title
Hepatology 44(1)
Pages: 240-251
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Reconstitution of hepatic tissue architectures from mouse liver cells using simulated microgravity culture system2007
Author(s)
Ueno, Y., Okamur, A., Zheng, Y.W., Tanaka, J., Tateisi, T., Tahiguchi, H
Organizer
The China-Japan Mini-Symposium 2007 on Nanotechnology and Tissue Enginieering
Place of Presentation
Japan
Year and Date
2007-12-08
Description
「研究成果報告書概要(欧文)」より
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[Book] 再生医孫工学の技術2007
Author(s)
谷口 英樹(分担執筆)
Total Pages
251
Publisher
シーエムシー出版
Description
「研究成果報告書概要(和文)」より
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