Treatment of central and/or peripheral nerve injury using artificial matrix and/or differentiated bone marrow stromal cell

• Project/Area Number: 17209056
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Plastic surgery
• Research Institution: Tazuke Kofukai Medical Research Institute (2006-2007); Kyoto University (2005)
• Principal Investigator: SUZUKI Yoshihisa Tazuke Kofukai Medical Research Institute, 3rd Division, Medical Research Institute, Chief Researcher (30243025)
• Co-Investigator(Kenkyū-buntansha): IDE Chizka Aino University, Department of Anatomy and Neurobiology, Professor (70010080) ; TANIHARA Masao Nara Institute of Science and Technology, Graduate School of Material Science, Professor (50294286)
• Project Period (FY): 2005 – 2007
• Project Status: Completed (Fiscal Year 2007)
• Budget Amount: ¥49,920,000 (Direct Cost: ¥38,400,000、Indirect Cost: ¥11,520,000); Fiscal Year 2007: ¥8,840,000 (Direct Cost: ¥6,800,000、Indirect Cost: ¥2,040,000); Fiscal Year 2006: ¥15,210,000 (Direct Cost: ¥11,700,000、Indirect Cost: ¥3,510,000); Fiscal Year 2005: ¥25,870,000 (Direct Cost: ¥19,900,000、Indirect Cost: ¥5,970,000)
• Keywords: central nerve / peripheral nerve / regeneration / bone marrow / spinal cord / 再生医療 / マトリクス / ペプチド / トランスレーショナル / 移植・再生医療 / 神経科学

Research Abstract

(1) The neurosphere cells were injected into the cerebrospinal fluid through the 4th ventricle of young rats that had been given a contusion injury at T8-9 of the spinal cord. The grafted stem cells survived well in the host spinal cord and were cell integrated into the host tissue, extending their processes around nerve fibers in the same manner as astrocyte.
(2) The spinal cord of the young rat was injured by contusion at the level of T8-9, and the BMSCs from the bone marrow were infused into the cerebrospinal fluid through the 4th ventricle. BMSCs attached to the spinal surface and a few invaded the lesion. The recovery of locomotion was better, and the cavity volume was smaller in the rat with implantation than in the control rats.
(3) Based on these studies, we aimed at the clinical application, and actually planned a clinical trial of spinal cord injury treatment by transplanting patient's autologous BMSCs into CSF. We have experienced one case. Motor and sensory scores gradually but apparently improved at 1 and 3 months compared with the scores before the transplantation.

Research Products

• [Journal Article] Spinal cord injury treatment with intrathecal autologous bone marrow stromal cell transplantation: the first clinical trial case report (2008)
  • Author(s): Saito,F.,Nakatani,T.,Iwase,M.,Maeda,Y.,Hirakawa,A.,Murao,Y.,Suzuki,Y.,Onodera,R.,Fukushima,M.,Ide,C.
  • Journal Title: The Journal of TRAUMA. 64 Pages: 53-59
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] The biodegradability of poly(Pro-Hyp-Gly) synthetic polypeptide and the promotion of a dermal wound epithelialization using a poly(Pro-Hyp-Gly) sponge (2008)
  • Author(s): Tanihara,M.,Kajiwara,K.,Suzuki,Y.,Kamitakahara,M.,Ogata,S.
  • Journal Title: Journal of Biomedical Materials Research. Part A. 85A Pages: 133-139
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Spinal cord injury treatment with intrathecal autologous bone marrow stromal cell transplantation : the first clinical trial case report (2008)
  • Author(s): Saito, F., Nakatani, T., Iwase, M., Maeda, Y., Hirakawa, A., Murao, Y., Suzuki, Y., Onodera, R., Fukushima, M., Ide, C.
  • Journal Title: The Journal of TRAUMA 64 Pages: 53-59
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] The biodgeradability of poly(Pro-Hyp-Gly) synthetic polypeptide and the promotion of a dermal wound epithelialization using a poly(Pro-Hyp-Gly) sponge (2008)
  • Author(s): Tanihara, M., Kajiwara, K., Szuki, Y., Kamitakahara, M., Ogata, S.
  • Journal Title: Journal of Biomedical Materials Research. Part A. 85A Pages: 133-139
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Peripheral nerve regeneration through the space formed by a chitosan gel sponge (2007)
  • Author(s): Ishikawa,N.,Suzuki,Y.,Ohta,M.,Cho,H.,Suzuki,S.,Dezawa,M.,Ide,C.
  • Journal Title: Journal of Biomedical Materials Research 83A Pages: 33-40
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Neuroprotective effect of bone marrow-derived mononuclear cells promoting functional recovery from spinal cord injury. (2007)
  • Author(s): Yoshihara,T.,Ohta,M.,Itokazu,Y.,Matsumoto,N.,Dezawa,M.,Suzuki,Y.,Taguci,A.,Watanabe,Y.,Adachi,Y.,Ikehara,S.,Sugimoto,Y.,Ide,C.
  • Journal Title: Journal of Neurotrauma. 24 Pages: 1026-1036
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Peripheral nerve regeneration through the space formed by a chitosan gel sponge (2007)
  • Author(s): Ishikawa, N., Suzuki, Y., Ohta, M., Cho, H., Suzuki, S., Dezawa, M., Ide, C.
  • Journal Title: Journal of Biomedical Materials Research. 83A Pages: 33-40
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Neuroprotective effect of bone marrw-derived mononuclear cells promoting functional recovery from spinal crd injury. (2007)
  • Author(s): Yoshihara T, Ohta M, Itokazu Y, Matumoto N, Dezawa M, Suzuki Y, Taniguchi A, Watanabe Y, Adachi Y, Ikehara S, Sugimoto H, Ide C
  • Journal Title: J. Neurotrauma 24 Pages: 1026-1036
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Repar of 20-mm long rabbit radial bone defects using BMP-derived peptide combined with an α-tricalcim phosphate scaffold (2006)
  • Author(s): Saito,A.,Suzuki,Y.,Kitamura,M.,Ogata,S.,Yoshihara,Y.,Masuda,S.,Ohtsuki,C.,Tanihara,M.
  • Journal Title: Journal of Biomedical Materials Research. Part A 77A Pages: 700-706
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Repar of 20-mm long rabbit radial bone defects using BMP-derived peptide combined with an α-tricalcim phosphate scaffold. (2006)
  • Author(s): Saito, A., Suzuki, Y., Kitamura, M., Ogata, S., Yoshihara, Y., Masuda, S., Ohtsuki, C., Tanihara, M.
  • Journal Title: Journal of Biomedical Materials Research. Part A. 77A Pages: 700-706
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Repar of 20-mm long rabbit radial bone defects using BMP-derived peptide combined with an a-tricalcim phosphate scaffold. (2006)
  • Author(s): Saito, A., Suzuki, Y., Kitamura, M., Ogata, S., Yoshihara, Y., Masuda, S., Ohtsuki, C., Tanihara, M.
  • Journal Title: Journal of Biomedical Materials Research. Part A. 77A Pages: 700-700
• [Journal Article] Accelerated bone repair with the use of a synthetic BMP-2-derived peptide and bone-marrow stromal cells (2005)
  • Author(s): Saito,A.,Suzuki,Y.,Ogata,S.,Ohtsuki,C.,Tanihara,M.
  • Journal Title: Journal of Biomedical Materials Research 72A Pages: 77-82
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Temporal skin grafts following straight incision for syndactyly correction (2005)
  • Author(s): Sawabe,K.,Suzuki,Y.,Suzuki,S.
  • Journal Title: Annals of Plastic Surgery 55 Pages: 139-142
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Review Peripheral nerve regeneration using non-tubular alginate gel crosslinked with covalent bonds (2005)
  • Author(s): Hashimoto,T.,Suzuki,Y.,Suzuki,K.,Nakashima,T.,Tanihara M.,Ide,C.
  • Journal Title: Journal of Materials Science:Materials in Medicine 16 Pages: 503-509
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Cell transplantation for neurodegenerative and neurotraumatic disorders (2005)
  • Author(s): Dezawa,M.,Matsumoto,N.,Ohta,M.,Itokazu,Y.,Suzuki,Y.,Ide,C.
  • Journal Title: Current Trends in Neurology 1 Pages: 38-49
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Accelerated bone repair with the use of a synthetic BMP-2-derived peptide and bone-marrow stromal cells. (2005)
  • Author(s): Saito, A., Suzuki, Y., Ogata, S., Ohtsuki, C. : Tanihara, M.
  • Journal Title: Journal of Biomedical Materials Research 72A Pages: 77-82
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Temporal skin grafts following straight incision for syndactyly correction (2005)
  • Author(s): Sawabe, K., Suzuki, Y., Suzuki, S.
  • Journal Title: Annals of Plastic Surgery 55 Pages: 139-142
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Review Peripheral nerve regeneration using non-tubular alginate gel crosslinked with covalent bonds. Journal of Materials Science (2005)
  • Author(s): Hashimoto, T., Suzuki, Y., Suzuki, K., Nakashima, T., Tanihara M., Ide, C.
  • Journal Title: Materials in Medicine 16 Pages: 503-509
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Cell transplantation for neurodegenerative and neurotraumatic disorders (2005)
  • Author(s): Dezawa, M., Matsumoto, N., Ohta, M., Itokazn, Y., Suzuki, Y., Ide, C.
  • Journal Title: Current Trends in Neurology 1 Pages: 38-49
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Review Peripheral nerve regeneration using non-tubular alginate gel crosslinked with covalent bonds (2005)
  • Author(s): Hashimoto, T., Suzuki, Y., Suzuki, K., Nakashima, T., Tanihara, M., Ide, C.
  • Journal Title: Journal of Materials Science : Materials in Medicine 16 Pages: 503-509
• [Journal Article] Accelerated bone repair with the use of a synthetic BMP-2-derived peptide and bone-marrow stromal cells (2005)
  • Author(s): Saito, A., Suzuki, Y., Ogata, S., Ohtsuki, C., Tanihara, M.
  • Journal Title: Journal of Biomedical Materials Research 72A Pages: 77-82
• [Journal Article] アルギン酸を用いた新しい神経再生用材料 (2005)
  • Author(s): 橋本正, 鈴木義久
  • Journal Title: 医学のあゆみ 215 Pages: 867-873
• [Journal Article] 脳脊髄液経由細胞移植法による脊髄損傷治療-特に骨髄間質細胞移植による脊髄損傷治療 (2005)
  • Author(s): 太田正佳, 鈴木義久, 井出千束
  • Journal Title: 細胞 37 Pages: 228-230
• [Journal Article] 再生医学的手法を用いた末梢神経再生 (2005)
  • Author(s): 太田正佳, 鈴木義久
  • Journal Title: PAPERS 3 Pages: 1-11
• [Journal Article] Transplantation of bone marrow stromal cells for nerve regeneration in CNS. Encyclopedic Reference in Neuroscience (Eds.MD Binder, N Hirokawa, U Windhorst)
  • Author(s): Ohta M.,Suzuki Y. and Ide C.
  • Journal Title: Springer-Verlag. (in press)
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Transplantation of artificial materials for nerve regeneration, Encyclopedic Reference in Neuroscience (Eds. MD Binder, N Hirokawa, U Windhorst)
  • Author(s): Ohta,M.,Suzuki,Y.,Ide,C.
  • Journal Title: Springer-Verlag. (in press)
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Transplantation of bone marrow stromal cells for nerve regeneration in CNS. Encyclopedic Reference in Neuroscience(Eds. MD Binder, N Hirokawa, U Windhorst).
  • Author(s): Ohta M., Suzuki Y., Ide C.
  • Journal Title: Springer-Verlag (in press)
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Transplantation of artificial materials for nerve regeneration, Encyclopedic Reference in Neuroscience(Eds. MD Binder, N Hirokawa, U Windhorst)
  • Author(s): Ohta, M., Suzuki, Y., Ide, C.
  • Journal Title: Springer-Verlag. (in press)
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Transplantation of bone marrow stromal cells for nerve regeneration in CNS.
  • Author(s): Ohta M., Suzuki Y., Ide C.
  • Journal Title: Encyclopedic Reference in Neuroscience (Eds.MD Binder, N Hirokawa, U Windhorst)Springer-Verlag. (in press)
• [Journal Article] Transplantation of artificial materials for nerve regeneration
  • Author(s): Ohta M., Suzuki Y., Ide C.
  • Journal Title: Encyclopedic Reference in Neuroscience (Eds.MD Binder, N Hirokawa, U Windhorst)Springer-Verlag. (in press)
• [Journal Article] Peripheral nerve regeneration through the space formed by a chitosan gel sponge
  • Author(s): Ishikawa, N., Suzuki, Y., Ohta, M., Cho, H., Suzuki, S., Dezawa, M., Ide, C.
  • Journal Title: Journal of Biomedical Materials Research. Part A (in press)
• [Presentation] From PNS regeneration to CNS regeneration (2006)
  • Author(s): Ide C.
  • Organizer: The 111th Annual Meeting of the Japanese Association of Anatomists
  • Place of Presentation: Kitazato University
  • Year and Date: 2006-03-30
  • Description: 「研究成果報告書概要(和文)」より
• [Book] ゲノム情報による医療材料の設計と開発 (2006)
  • Author(s): 谷原正夫
  • Total Pages: 250
  • Publisher: シーエムシー出版
• [Patent(Industrial Property Rights)] 脊髄損傷の治療および/または予防法 (2005)
  • Inventor(s): 鈴木義久、井出千束、吉原智之、太田正佳
  • Industrial Property Rights Holder: 国立大学法人京都大学
  • Filing Date: 2005-08-08
  • Description: 「研究成果報告書概要(和文)」より
  • Overseas