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Accurate and Fast 3D Image Reconstruction in Fluorescence Microscopy and Automatic labeling of 3D tissue structures

Research Project

Project/Area Number 17300061
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Perception information processing/Intelligent robotics
Research InstitutionTokyo Institute of Technology

Principal Investigator

KUMAZAWA Itsuo  Tokyo Institute of Technology, Interdisciplinary Graduate School of Science and Engineering, Professor, 大学院・理工学研究科, 教授 (70186469)

Co-Investigator(Kenkyū-buntansha) NAGAHASHI Hiroshi  Tokyo Institute of Technology, Interdisciplinary Graduate School of Science and Engineering, Professor, 大学院・理工学研究科, 教授 (20143084)
MOROOKA Kenichi  Kyushu University, Digital Medicine Initiative, Associate Professor, デジタルメディシン・イニシアティブ, 助教授 (80323806)
Project Period (FY) 2005 – 2006
Project Status Completed (Fiscal Year 2006)
Budget Amount *help
¥15,600,000 (Direct Cost: ¥15,600,000)
Fiscal Year 2006: ¥6,000,000 (Direct Cost: ¥6,000,000)
Fiscal Year 2005: ¥9,600,000 (Direct Cost: ¥9,600,000)
KeywordsFluorescence Microscope / Three dimensional image / Deconvolution / Image Matching / EM method / Image reconstruction / Three dimensional shape model / Shape matching / 3次元映像化 / Nearest Neighbor法 / 3次元形状モデリング / Point Spread Function / 画像復元 / 断面画像
Research Abstract

This project has been aiming to evaluate and improve the new three dimensional reconstruction algorithm developed for Fluorescence Microscopy that has been proven effective for artificially generated ideal data in our previous project. In order to evaluate the effectiveness of the algorithm for actual data observed from tissue structures, we corrected actual data using a fluorescence microscope and have conducted comprehensive evaluation using the data under different conditions by changing noise level and a coupled of optical parameters. Through this evaluation, we found that the new algorithm is sensitive to PSF (Point Spread Function) parameters and, affected by non-transparent parts that contradicts the ideal situation that the deconvolution-based reconstruction theory assumes. We have spent the most of our time to solve this problem and concluded that reconstructed results would not be improved as far as using deconvolution-based-algorithm and it is necessary to develop a method that would not assume transparency for the objects. After the evaluation and adding some improvement, we also tried to develop matching algorithms for three dimensional structures between actual tissue structures observed by Fluorescence Microscopy and artificially synthesized three dimensional structures. We have examined an initial three dimensional shape modeled by a polyhedron would converge to the target shape by iteration procedure using a neural network specifically designed for the purpose.

Report

(3 results)
  • 2006 Annual Research Report   Final Research Report Summary
  • 2005 Annual Research Report
  • Research Products

    (16 results)

All 2007 2006 2005

All Journal Article (16 results)

  • [Journal Article] 視差情報を援用する蛍光顕微鏡画像の3次元再構成2007

    • Author(s)
      栗田俊, 熊澤逸夫
    • Journal Title

      情報処理学会・コンピュータビジョンとイメージメディア研究会 158-8

      Pages: 372-377

    • NAID

      110006250818

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Annual Research Report 2006 Final Research Report Summary
  • [Journal Article] ユーザインタフェースのためのオブジェクト追跡手法に関する研究2007

    • Author(s)
      宮平陽栄, 熊澤逸夫
    • Journal Title

      情報処理学会・コンピュータビジョンとイメージメディア研究会 158-10

      Pages: 383-388

    • NAID

      110006250820

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Annual Research Report 2006 Final Research Report Summary
  • [Journal Article] エッジの幾何学的特徴を用いたカラー画像の色領域分割に関する研究2007

    • Author(s)
      野村 一仁, 熊澤 逸夫
    • Journal Title

      精密工学会・動的画像処理実利用化ワークショップ2007 05-2

      Pages: 172-177

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Annual Research Report 2006 Final Research Report Summary
  • [Journal Article] Simultaneously Reconstructing Transparent and Opaque Surfaces from Texture Images2007

    • Author(s)
      Mohamad Ivan Fanany, Itsuo Kumazawa
    • Journal Title

      Proceedings of the 5th International Conference on Vision Systems CD-ROM

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Three Dimensional Reconstruction of Fluorescence Microscope images using disparity among stereo images2007

    • Author(s)
      Shun Kurita, Itsuo Kumazawa
    • Journal Title

      Information Processing Society, Research Group on Computer Vision and Image Media 158-8

      Pages: 372-377

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Object tracking methods for user interface2007

    • Author(s)
      Youei Miyahira, Itsuo Kumazawa
    • Journal Title

      Information Processing Society, Research Group on Computer Vision and Image Media 158-10

      Pages: 383-388

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Area separation by color and geometrical features of edges2007

    • Author(s)
      Kazuhito Nomura, Itsuo Kumazawa
    • Journal Title

      Workshop on Dynamic Image processing for real Application

      Pages: 172-177

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Simultaneously Reconstructing Transparent and Opaque S urfaces from Texture Images2007

    • Author(s)
      Mohamad Ivan Fanany, Itsuo Kumazawa
    • Journal Title

      Proceedings of the 5th International Conference on Vision Systems (CD-ROM)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Simultaneously Reconstructing Transparent and Opaque Surfaces from Texture Images2007

    • Author(s)
      Mohamad Ivan Fanany, Ituo Kumazawa
    • Journal Title

      Proceedings of the 5th International Conference on Vision Systems (CD-ROM)

    • Related Report
      2006 Annual Research Report
  • [Journal Article] 他断面からの光の混入を除去した蛍光顕微鏡画像の再構成手法2006

    • Author(s)
      伊藤隆泰, 熊澤逸夫
    • Journal Title

      電子情報通信学会・データ工学研究会 DE2006-21

      Pages: 61-66

    • NAID

      110004751970

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Annual Research Report 2006 Final Research Report Summary
  • [Journal Article] パターンマッチングにおけるエッジ情報の安定性の評価および安定したエッジの抽出に関する研究2006

    • Author(s)
      高畑知也, 宮平陽栄, 熊澤逸夫
    • Journal Title

      電気学会,情報処理・産業システム情報化合同研究会 IIS-06-29

      Pages: 13-18

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Annual Research Report 2006 Final Research Report Summary
  • [Journal Article] Reconstruction of Section Images in Fluorescence Microscopy Reducing Crosstalk from Unfocused Sections2006

    • Author(s)
      Takayasu Itoh, Itsuo Kumazawa
    • Journal Title

      IEICE Technical Report DE2006-21, PRMU2006-59

      Pages: 61-66

    • NAID

      110004751970

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Evaluation of edge stability and extraction of stable edges for fast pattern matching2006

    • Author(s)
      Tomoya Takahata, Youei Miyahara, Itsuo Kumazawa
    • Journal Title

      The papers of Joint Technical Meeting on Information Processing and Information Oriented Industrial System (IEE Japan) IIS-06-29

      Pages: 13-18

    • NAID

      10019290711

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] A Neural Network Scheme for Transparent Surface Modeling2005

    • Author(s)
      Mohamad Ivan Fanany, Itsuo Kumazawa, Kikuchi Kobayashi
    • Journal Title

      Proceedings of GRAHITE 2005

      Pages: 433-438

    • Related Report
      2005 Annual Research Report
  • [Journal Article] 微小視差のサブピクセル計測から求められる3次元距離画像の精度2005

    • Author(s)
      熊澤逸夫, 張暁林, 佐藤誠
    • Journal Title

      第11回画像センシングシンポジウム講演論文集

      Pages: 61-64

    • Related Report
      2005 Annual Research Report
  • [Journal Article] 両眼固視微動を用いた立体エッジ画像生成法2005

    • Author(s)
      張暁林, 川合拓郎, 熊澤逸夫, 佐藤誠
    • Journal Title

      第11回画像センシングシンポジウム講演論文集

      Pages: 303-306

    • Related Report
      2005 Annual Research Report

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Published: 2005-04-01   Modified: 2016-04-21  

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