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Project to prove a link between phosphorylation of one amino-acid in glutamate receptors and motor learning

Research Project

Project/Area Number 17300100
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Neuroscience in general
Research InstitutionGunma University (2006)
Kanazawa University (2005)

Principal Investigator

HIRAI Hirokazu  GUNMA UNIVERSITY, GRADUATE SCHOOL OF MEDICINE, PROFESSOR, 大学院・医学系研究科, 教授 (70291086)

Co-Investigator(Kenkyū-buntansha) YANAGIHARA Dai  UNIVERSITY OF TOKYO, GRADUATE SCHOOL OF ARTS AND SCIENCES, 大学院・総合文化研究科, 助教授 (90252725)
Project Period (FY) 2005 – 2006
Project Status Completed (Fiscal Year 2006)
Budget Amount *help
¥15,200,000 (Direct Cost: ¥15,200,000)
Fiscal Year 2006: ¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 2005: ¥12,200,000 (Direct Cost: ¥12,200,000)
KeywordsPurkinje cell / Glutamate receptor / Transgenic mouse / GluR2 / Cerebellum
Research Abstract

Tropism of lentiviral vectors for Purkinje cells of a mouse in vivo is influenced by cultivating condition of HEK293T cells, host cells for viral production. The tropism for Purkinje cells is high when the pH of the culture medium is >7.2, whereas the lentiviral vectors produced in the medium with the pH of <7.0 has high tropism for Bergmann glia.
We aimed to produce gene-modified mice in which phosphorylation of GluR2 Ser880 is impaired specifically in Purkinje cells. This is attained by crossing 2 different transgenic mice; A mouse expressing L7-rtTA and that express GluR2 K882A under the control of tetracycline-responsive (TRE) promoter. Expression of rtTA in the former mouse is driven by Purkinje-cell-specific L7 promoter. The transgenic mouse having both transgenes expresses GluR2 K882A only when doxycycline is administered through water and food. We obtained 6 lines of mice with L7-rtTA transgene, in which only 2 lines showed rtTA expression in Purkinje cells. While 4 lines of mice with TRE promoter-GluR2 K882 and 3 lines of mice with TRE promoter-wild-type GluR2 were obtained. Now we are increasing the number of those mice for crossing them with L7-rtTA mice.

Report

(3 results)
  • 2006 Annual Research Report   Final Research Report Summary
  • 2005 Annual Research Report
  • Research Products

    (24 results)

All 2007 2006 2005

All Journal Article (20 results) Book (2 results) Patent(Industrial Property Rights) (2 results)

  • [Journal Article] Production of neuron-preferential lentiviral vectors.2007

    • Author(s)
      Torashima T, Koyama C, Higashida H, Hirai H
    • Journal Title

      Mature Protocols

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Progress in transduction of cerebellar Purkinje cells in vivo using viral vectors.2007

    • Author(s)
      Hirai H.
    • Journal Title

      Cerebellum (In press)

    • NAID

      120000807297

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Exposure of lentiviral vectors to subneutral pH shifts the tropism from Purkinje cell to Bergmann glia.2007

    • Author(s)
      Torashima T, Yamada N, Itoh M, Yamamoto A, Hirai H.
    • Journal Title

      Nature Protocols

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Production of neuron-preferential lentiviral vectors.2007

    • Author(s)
      Torashima T, Koyama C, Higashida H, Hirai H.
    • Journal Title

      Cerebellum (In press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] New concepts regulating synaptic transmission and plasticity.2007

    • Author(s)
      Hirai H, Kano M.
    • Journal Title

      KUBA PRO. Brain Science Review

      Pages: 123-140

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] In vivo transduction of murine cerebellar Purkinje cells by HIV-derived lentiviral vectors2006

    • Author(s)
      Torashima T, Okoyama S, Nishizaki T, Hirai H
    • Journal Title

      Brain Research 1082

      Pages: 11-22

    • NAID

      130007038943

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Exposure of lentiviral vectors to subneutral pH shifts the tropism from Purkinje cell to Bergmann glia2006

    • Author(s)
      Torashima T, Yamada N, Itoh M, Yamamoto A, Hirai H
    • Journal Title

      European Journal of Neuroscience 24

      Pages: 371-380

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Annual Research Report 2006 Final Research Report Summary
  • [Journal Article] 小脳シナプス形成とシナプス可塑性を制御する分子・細胞メカニズム2006

    • Author(s)
      平井宏和
    • Journal Title

      実験医学 24(No. 15)(増刊)

      Pages: 2402-2409

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] 神経細胞選択的なHIV由来レンチウィルスベクターの作製法2006

    • Author(s)
      平井宏和
    • Journal Title

      実験医学 24

      Pages: 2997-3001

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] In vivo transduction of murine cerebellar Purkinje cells by HIV-derived lentiviral vectors.2006

    • Author(s)
      Torashima T, Okoyama S, Nishizaki T, Hirai H.
    • Journal Title

      European Journal of Neuroscience 24

      Pages: 371-380

    • NAID

      130007038943

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Elucidation of the cerebellar function by generating2006

    • Author(s)
      Hirai H.
    • Journal Title

      Experimental Medicine 24(No.15)

      Pages: 2402-2409

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Molecular and cellular mechanisms regulating synaptogenesis and synaptic plasticity in the cerebellum.2006

    • Author(s)
      Hirai H.
    • Journal Title

      Experimental Medicine 24

      Pages: 2997-3001

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Phosphorylation of the glutamate receptor and motor learning.2006

    • Author(s)
      Hirai H.
    • Journal Title

      Brain Research 1082(1)

      Pages: 11-22

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] In vivo transduction of murine cerebellar Purkinje cells by HIV-derived lentiviral vectors2006

    • Author(s)
      Torashima T, Okoyama S, Nishizaki T, Hirai H.
    • Journal Title

      Brain Research 1082

      Pages: 11-22

    • NAID

      130007038943

    • Related Report
      2006 Annual Research Report
  • [Journal Article] 神経細胞選択的なHIV由来レンチウィルスベクターの作製法2006

    • Author(s)
      平井 宏和
    • Journal Title

      実験医学 24

      Pages: 2997-3001

    • Related Report
      2006 Annual Research Report
  • [Journal Article] 小脳シナプス形成とシナプス可塑性を制御する分子・細胞メカニズム2006

    • Author(s)
      平井 宏和
    • Journal Title

      実験医学 24

      Pages: 2402-2409

    • Related Report
      2006 Annual Research Report
  • [Journal Article] In vivo transduction of murine cerebellar Purkinje cells by HIV-derived lentiviral vectors.2006

    • Author(s)
      寅嶋 崇
    • Journal Title

      Brain Research (In press)

    • Related Report
      2005 Annual Research Report
  • [Journal Article] 遺伝子レスキューマウス作出による小脳の運動学習機構の解明2005

    • Author(s)
      平井宏和
    • Journal Title

      実験医学 23(8)

      Pages: 1170-1175

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Progress in transduction of cerebellar Purkinje cells in vivo using viral vectors.2005

    • Author(s)
      Hirai H.
    • Journal Title

      Experimental Medicine 23(8)

      Pages: 1170-1175

    • NAID

      120000807297

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Production of HIV-derived lentiviral vectors with high tropism for neurons.2005

    • Author(s)
      Hirai H.
    • Journal Title

      YODOSHA brain-neuroscience intensive master

      Pages: 70-76

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Book] ブレインサイエンス レビュー2007

    • Author(s)
      平井宏和
    • Publisher
      クバプロ
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Book] 脳・神経科学集中マスター2005

    • Author(s)
      平井宏和, 狩野方伸
    • Publisher
      羊土社
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Patent(Industrial Property Rights)] プルキンエ細胞指向性ウイルスベクター2007

    • Inventor(s)
      平井宏和, 寅嶋崇
    • Industrial Property Rights Holder
      金沢大学
    • Filing Date
      2007-03-07
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Patent(Industrial Property Rights)] プルキンエ細胞指向性ウイルスベクター2006

    • Inventor(s)
      平井宏和, 寅嶋崇
    • Industrial Property Rights Holder
      金沢大学
    • Industrial Property Number
      2006-198398
    • Filing Date
      2006-07-20
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary

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Published: 2005-04-01   Modified: 2016-04-21  

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