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Mechanism of fore-mid-brain determination by XSa1F

Research Project

Project/Area Number 17300107
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Neuroscience in general
Research InstitutionRIKEN

Principal Investigator

SASAI Yoshiki  RIKEN, Organogenesis and Neurogenesis Group, Group Director, 細胞分化・器官発生研究グループ, グループデイレクター (20283616)

Project Period (FY) 2005 – 2006
Project Status Completed (Fiscal Year 2006)
Budget Amount *help
¥14,900,000 (Direct Cost: ¥14,900,000)
Fiscal Year 2006: ¥7,200,000 (Direct Cost: ¥7,200,000)
Fiscal Year 2005: ¥7,700,000 (Direct Cost: ¥7,700,000)
KeywordsNeural development / patterning / early embryo / Xenopus / XSa1F / XTsh3 / Wnt / anterior-posterior axis / 神経分化 / 脳発生 / 前脳 / 中脳 / Sal / 転写因子 / Tsh3
Research Abstract

The aim of this study is to understand molecular mechanisms of the early vertebrate embryo, particularly in the nervous system, by focusing on the roles of zinc-finger proteins and their related molecules in the rostral neuroectodermal development of Xenopus. We had previously isolated the zinc-finger factor XSa1F, which plays an essential role in the determination of the rostral brain. In this study, we identified another zinc-finger transcription factor, XTsh3, which is expressed in the Xenopus caudal nervous system. We showed that XTsh3 is a promoting factor required for the proper development of the caudal neural tissues. Overexpression of XTsh3 caused caudalization of the neural tissues and suppressed forebrain development. Conversely, XTsh3-MO injection, which blocks its function, enhanced forebrain development. In signaling analysis, we found that XTsh3 augments Wnt/b-catenin signaling. XTsh3 binds to both b-catenin and Tcf3, and amplifies Wnt-induced transcription. Consistent with these observations, XTsh3-MO injection into mesoendodermal tissues inhibited dorsal development, leading to strong ventralization of the whole embryo. Thus, in addition to XSa1F, which acts as a negative modulator of Wnt signaling, XTsh3 plays an important role in the fine modulation of the Wnt pathway. The antagonistic pair of XSa1F and XTsh3 is likely to function as a binary switching system of Wnt-On vs.-Off regulations.

Report

(3 results)
  • 2006 Annual Research Report   Final Research Report Summary
  • 2005 Annual Research Report
  • Research Products

    (21 results)

All 2006 2005

All Journal Article (21 results)

  • [Journal Article] Essential Pro-BMP Roles of Crossveinless2 in Mouse Organogenesis.2006

    • Author(s)
      Makoto Ikeya
    • Journal Title

      Development 133

      Pages: 4463-4473

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Neural Conversion of Embryonic Stem Cells by an Inductive Activity on Human Amniotic Membrane Matrix.2006

    • Author(s)
      Morio Ueno
    • Journal Title

      Proceeding of the National Academy of Science of the United States id America 103

      Pages: 9554-9559

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Generation of graftable dopaminergic neuron progenitors from mouse ES cells by a combination of coculture and neurosphere methods2006

    • Author(s)
      Asuka Morizane
    • Journal Title

      Journal of Neuroscience Research 83

      Pages: 1015-1027

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Annual Research Report 2006 Final Research Report Summary
  • [Journal Article] One-Step Induction of Neurons From Mouse Embryonic Stem Cells in Serum-Free Media Containing Vitamin B12 and Heparin.2006

    • Author(s)
      Hironori Yamazoe
    • Journal Title

      Cell Transplantation 15

      Pages: 135-145

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Annual Research Report 2006 Final Research Report Summary
  • [Journal Article] Generation of Cerebellar Neuron Precursors from Embryonic Stem Cells.2006

    • Author(s)
      Su Hong Lin
    • Journal Title

      Developmental Biology 290

      Pages: 287-296

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Annual Research Report 2006 Final Research Report Summary
  • [Journal Article] One-Step Induction of Neurons From Mouse Embryonic Stem Cells in Serum-Free Media Containing Vitamin B! and Heparin.2006

    • Author(s)
      Hironori Yamazoe
    • Journal Title

      Cell Transplantation 15

      Pages: 135-145

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Essential Pro-BMP Roles of Crossveinless 2 in Mouse Organogenesis.2006

    • Author(s)
      Makoto Ikeya
    • Journal Title

      Development 133

      Pages: 4463-4473

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Neural Conversion of Embryonic Stem Cells by an Inductive Activity on Human Amniotic Membrane Matrix2006

    • Author(s)
      Morio Ueno
    • Journal Title

      Proceeding of the National Academy of Science of the United States of America 103

      Pages: 9554-9559

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Generation of Rx+/Pax6+ neural retinal precursors from embryonic stem cells.2005

    • Author(s)
      Hanako Ikeda
    • Journal Title

      Proceedings of the National Academy of Sciences of the United States of America 102・32

      Pages: 11331-11336

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] An essential role of Xenopus Foxila for ventral specification of the cephalic ectoderm during gastrulation.2005

    • Author(s)
      Mami Matsuo-Takasaki
    • Journal Title

      Development 132

      Pages: 3885-3894

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Gene Disruption/Knock-in Analysis of mONT3 : Vector Construction by Combining in vivo Recombinations.2005

    • Author(s)
      Makoto Ikeya
    • Journal Title

      The International Journal of Developmental Biology 49

      Pages: 807-823

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Neural Crest Determination by Co-Activation of Pax3 and Zicl Genes in Xenopus Ectoderm.2005

    • Author(s)
      Takahiko Sato
    • Journal Title

      Development 132

      Pages: 2355-2363

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Directed differentiation of telencephalic precursors from embryonic stem cells.2005

    • Author(s)
      Kiichi Watanabe
    • Journal Title

      Nature Neuroscience 8

      Pages: 288-296

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary 2005 Annual Research Report
  • [Journal Article] Generation of RX+/Pax6+ neural precursors from embryonic stem cells2005

    • Author(s)
      Hanako Ikeda
    • Journal Title

      Proceedings of the National Academy of Sciences of the United States of America 102(32)

      Pages: 11331-11336

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] An essential role of Xenopus Foxila for ventral specification of the cephalic ectoderm during gastrulation.2005

    • Author(s)
      Mami Matsuo-Tahasaki
    • Journal Title

      Development 132

      Pages: 3885-3894

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Gene Disruption/Knock-in Analysis of mONT3 : Vector Construction by Combining in vivo Recombinations2005

    • Author(s)
      Makoto Ikeya
    • Journal Title

      the International Journal of Developmental Biology 49

      Pages: 807-823

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Neural Crest Determination by Co-Activation of Pax3 and Zic1 Genes in Xenopus Ectoderm.2005

    • Author(s)
      Takahiko Sato
    • Journal Title

      Development 132

      Pages: 2355-2363

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Generation of Rx+/Pax6+ neural retinal precursors from embryonic stem cells.2005

    • Author(s)
      Hanako Ikeda
    • Journal Title

      Proceedings of the National Academy of Sciences of the United States of America 102,32

      Pages: 11331-11336

    • Related Report
      2005 Annual Research Report
  • [Journal Article] An essential role of Xenopus Foxila for ventral specification of the cephalic ectoderm during gastrulation2005

    • Author(s)
      Mami Matsuo-Takasaki
    • Journal Title

      Development 132

      Pages: 3885-3894

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Gene Disruption/Knock-in Analysis of mONT3 : Vector Construction by Combining in vivo and in vitro Recombinations2005

    • Author(s)
      Makoto Ikeya
    • Journal Title

      The International Journal of Developmental Biology 49

      Pages: 807-823

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Neural Crest Determination by Co-activation of Pax3 and Zic1 Genes in Xenopus Ectoderm.2005

    • Author(s)
      Takahiko Sato
    • Journal Title

      Development 132

      Pages: 2355-2363

    • Related Report
      2005 Annual Research Report

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Published: 2005-04-01   Modified: 2016-04-21  

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