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Molecular mechanism underlying promotion of neuronal regeneration

Research Project

Project/Area Number 17300113
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Nerve anatomy/Neuropathology
Research InstitutionOsaka City University

Principal Investigator

KIYAMA Hiroshi  Osaka City University, Graduate School of Medicine, Professor, 大学院医学研究科, 教授 (00192021)

Co-Investigator(Kenkyū-buntansha) KIRYU-SEO Sumiko  Osaka City University, Graduate School of Medicine, Lecturer, 大学院医学研究科, 講師 (70311529)
MAEDA Mitsuyo  Osaka City University, Graduate School of Medicine, Lecturer, 大学院医学研究科(平成18年8月まで), 講師 (40122080)
NAKAGOMI Saya  Osaka City University, Graduate School of Medicine, Research Associate, 大学院医学研究科, 助手 (60423894)
Project Period (FY) 2005 – 2006
Project Status Completed (Fiscal Year 2006)
Budget Amount *help
¥15,000,000 (Direct Cost: ¥15,000,000)
Fiscal Year 2006: ¥6,500,000 (Direct Cost: ¥6,500,000)
Fiscal Year 2005: ¥8,500,000 (Direct Cost: ¥8,500,000)
KeywordsATF3 / cJun / STAT3 / Neuronal regeneration / hypoglossal nerve / Akt
Research Abstract

This study was carried out to investigate "Molecular mechanism underlying promotion of neuronal regeneration". Two major topics were investigated : (1) Identification of transcription factors and epigenetic factors, which are associated with neuronal regeneration, (2) Identification of protein modification, which promotes neuronal regeneration. We have revealed that transcription factors such as ATF3, cJun and STAT3 were crucial to promote nerve regeneration, and further tried to understand the interaction of those transcription factors using the promoter for one of nerve regeneration associated molecules, damage induced neuronal endopeptidase DINE. This analysis demonstrated that those transcription factors seemed not to bind the promoter region directly, and instead suggested the existence of a sort of platform molecule for the assembly of those transcription factors. The transcriptional control identified in this study may be specific to nerve regeneration and would provide a novel … More transcription mechanism for the promotion of nerve regeneration. We have also attempted to identify protein modifications such as phosphorylation, methylation, and acetylation for the promotion of nerve regeneration. To identify the modified molecules in response to nerve injury, we used 2-DG electrophoresis and blotted with antibodies, which recognized a certain kinase specific phosphorylation site for instance. The candidate spots, which showed up-regulation of the modification were further analyzed by TOFMAS. We have identified pheripherin as a phosphorylated molecule by Akt in response to nerve injury. Although the functional significance of the phosphorylation is not clear, the method established in this experiment could be a good tool for the identification of the proteins, which are modified by phosphorylation, methylation, or acetylation. The results obtained in the present project provide us with a novel transcription mechanism underlying the nerve regeneration, and also provide useful tool for the identification of molecular modification by phosphorylation, methylation, and acetylation. Less

Report

(3 results)
  • 2006 Annual Research Report   Final Research Report Summary
  • 2005 Annual Research Report
  • Research Products

    (33 results)

All 2006 2005

All Journal Article (33 results)

  • [Journal Article] GTP Hydrolysis by the Rho Family GTPase TC10 Promotes Exocytic Vesicle Fusion.2006

    • Author(s)
      Kawase K, Nakamura T, Takaya A, Aoki K, Namikawa K, Kiyama H, Inagaki S, Takemoto H, Saltiel AR, Matsuda M.
    • Journal Title

      Dev Cell 11(3)

      Pages: 411-421

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Unique anti-apoptotic activity of EAAC1 in injured motor neurons2006

    • Author(s)
      Kiryu-Seo S, Gamo K, Tachibana T, Tanaka K, Kiyama H
    • Journal Title

      EMBO J 25(14)

      Pages: 3411-3421

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Annual Research Report 2006 Final Research Report Summary
  • [Journal Article] Pancreatitis-associated protein-III (PAP-III) is a novel macrophage chemoattractant implicated in nerve regeneration2006

    • Author(s)
      Namikawa K, Okamoto T, Suzuki A, Konishi H, Kiyama H
    • Journal Title

      J Neurosci 26(28)

      Pages: 7460-7467

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Annual Research Report 2006 Final Research Report Summary
  • [Journal Article] Nerve injury induces the expression of EXT2, a glyc osyltransferase required for heparan sulfate synthesis2006

    • Author(s)
      Murakami K, Namikawa K, Shimizu T, Shirasawa T, Yoshida S, Kiyama H
    • Journal Title

      Neuroscience 141(4)

      Pages: 1961-1969

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Localization and ontogeny of damage-induced neuronal endopeptidase (DINE) mRNA-expressing neurons in the rat nervous system2006

    • Author(s)
      Nagata K, Kiryu-Seo S, Kiyama H
    • Journal Title

      Neuroscience 141(1)

      Pages: 299-310

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Annual Research Report 2006 Final Research Report Summary
  • [Journal Article] Targeted gene therapy toward astrocytoma using a Cre/loxP-based adenovirus system2006

    • Author(s)
      Maeda M, Namikawa K, Kobayashi I, Ohba N, Takahara Y, Kadono C, Tanaka A, Kiyama H
    • Journal Title

      Brain Res 1081

      Pages: 34-43

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Related Articles, Links Identification and functional characterization of mouse TPO1 as a myelin membrane protein.2006

    • Author(s)
      Fukazawa N, Ayukawa K, Nishikawa K, Ohashi H, Ichihara N, Hikawa Y, Abe T, Kudo Y, Kiyama H, Wada K, Aoki S.
    • Journal Title

      Brain Res. 1070

      Pages: 1-14

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] A newly modified SCG10 promoter and Cre/loxP-mediated gene amplification system achieve highly specific neuronal expression in animal brains2006

    • Author(s)
      Namikawa K, Murakami K, Okamoto T, Okado H, Kiyama H
    • Journal Title

      Gene Therapy 13(16)

      Pages: 1244-1250

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Annexin III implicated in the microglial response to motor nerve injury.2006

    • Author(s)
      Konishi H, Namikawa K, Kiyama H
    • Journal Title

      Glia 53(7)

      Pages: 723-732

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Age related alteration of neurturin receptor GFRa2 and nNOS in pelvic ganglia.2006

    • Author(s)
      Hisasue S, Kato R, Suetomi T, Kato K, Suzuki K, Kobayashi K, Itoh N, Kiyama H, Tsukamoto T
    • Journal Title

      Neurobiol Aging 27

      Pages: 1524-1530

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] GTP Hydrolysis by the Rho Family GTPase TC10 Promotes Exocytic Vesicle Fusion.2006

    • Author(s)
      Kawase K et al.
    • Journal Title

      Dev Cell 11(3)

      Pages: 411-421

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Unique anti-apoptotic activity of EAAC1 in injured motor neurons2006

    • Author(s)
      Kiryu-Seo S, et al.
    • Journal Title

      EMBO J25(14)

      Pages: 3411-3421

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Pancreatitis-associated protein-III (PAP-III) is a novel macrophage chemoattractant implicated in nerve regeneration2006

    • Author(s)
      Namikawa K, et al.
    • Journal Title

      JNeurosci 26(28)

      Pages: 7460-7467

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Nerve injury induces the expression of EXT2, a glycosyltransferase required for heparan sulfate synthesis2006

    • Author(s)
      Murakami K, et al.
    • Journal Title

      Neuroscience 141(4)

      Pages: 1961-1969

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Localization and ontogeny of damage-induced neuronal endopeptidase (DINE) mRNA-expressing neurons in the rat nervous system2006

    • Author(s)
      Nagata K, et al.
    • Journal Title

      Neuroscience 141(1)

      Pages: 299-310

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Targeted gene therapy toward astrocytoma using a Cre/loxP-based adenovirus system2006

    • Author(s)
      Maeda M, et al.
    • Journal Title

      Brain Res 1081

      Pages: 34-43

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Related Articles, Links Identification and functional characterization of mouse TPO1 as a myelin membrane protein.2006

    • Author(s)
      Fukazawa N, et al.
    • Journal Title

      Brain Res. 1070(1)

      Pages: 1-14

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] A newly modified SCG10 promoter and Cre/loxP-mediated gene amplification system achieve highly specific neuronal expression in animal brains2006

    • Author(s)
      Namikawa K, et al.
    • Journal Title

      Gene Therapy 13(16)

      Pages: 1244-1250

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Annexin III implicated in the microglial response to motor nerve injury.2006

    • Author(s)
      Konishi H, et al.
    • Journal Title

      Glia 53(7)

      Pages: 723-732

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Age related alteration of neurturin receptor GFRa2 and nNOS in pelvic ganglia.2006

    • Author(s)
      Hisasue S, et al.
    • Journal Title

      Neurobiol Aging 27

      Pages: 1524-1530

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] GTP Hydrolysis by the Rho Family GTPase TC10 Promotes Exocytic Vesicle Fusion.2006

    • Author(s)
      Kawase K, Nakamura T, Takaya A, Aoki K, Namikawa K, Kiyama H, Inagaki S, Takemoto H, Saltiel AR, Matsuda M
    • Journal Title

      Dev Cell 11(3)

      Pages: 411-421

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Nerve injury induces the expression of EXT2, a glycosyltransferase required for heparan sulfate synthesis2006

    • Author(s)
      Murakami K, Namikawa K, Shimizu T, Shirasawa T, Yoshida S, Kiyama H
    • Journal Title

      Neuroscience 141(4)

      Pages: 1961-1969

    • Related Report
      2006 Annual Research Report
  • [Journal Article] A newly modified SCG10 promoter and Cre/IoxP-mediated gene amplification system achieve highly specific neuronal expression in animal brains2006

    • Author(s)
      Namikawa K, Murakami K, Okamoto T, Okado H, Kiyama H
    • Journal Title

      Gene Therapy 13(16)

      Pages: 1244-1250

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Induced expression of Rab24 GTPase and LC3 in nerve-injured motor neurons.2005

    • Author(s)
      Egami Y, Kiryu-Seo S, Yoshimura T, Kiyama H
    • Journal Title

      Biochem Biophys Res Commun 337

      Pages: 1206-1213

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary 2005 Annual Research Report
  • [Journal Article] Cell Type-Specific Intervention of Transforming Growth Factor beta/Smad Signaling Suppresses Collagen Gene Expression and Hepatic Fibrosis in Mice.2005

    • Author(s)
      Inagaki Y, Kushida M, Higashi K, Itoh J, Higashiyama R, Hong YY, Kawada N, Namikawa K, Kiyama H, Bou-Gharios G, Watanabe T, Okazaki I, Ikeda K
    • Journal Title

      Gastroenterology 129

      Pages: 259-268

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Expression of Reg/PAP family members during motor nerve regeneration in rat.2005

    • Author(s)
      Namikawa K, Fukushima M, Murakami K, Suzuki A, Takasawa S, Okamoto H, Kiyama H
    • Journal Title

      Biochem Biophys Res Commun 332

      Pages: 126-134

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary 2005 Annual Research Report
  • [Journal Article] The p53-independent nuclear translocation of Cyclin G1 in degenerating neurons by ischemic and traumatic insults.2005

    • Author(s)
      Maeda M, Ampo K, Kiryu-Seo S, Konishi H, Ohba N, Kadono C, Kiyama H
    • Journal Title

      Exp Neurol 193(2)

      Pages: 350-360

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary 2005 Annual Research Report
  • [Journal Article] Noxa is a critical mediator of p53-dependent motor neuron death after nerve injury in adult mouse.2005

    • Author(s)
      Kiryu-Seo S, Hirayama T, Kato R, Kiyama H
    • Journal Title

      J Neurosci 25(6)

      Pages: 1442-1447

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Cell Type-Specific Intervention of Transforming Growth Factor beta/Smad Signaling Suppresses Collagen Gene Expression and Hepatic Fibrosis in Mice.2005

    • Author(s)
      Inagaki Y, et al.
    • Journal Title

      Gastroenterology 129(1)

      Pages: 259-268

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Expression of Reg/PAP family members during motor nerve regeneration in rat.2005

    • Author(s)
      Namikawa K, et al.
    • Journal Title

      Biochem Biophys Res Commun 332

      Pages: 126-134

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Induced expression of Rab24 GTPase and LC3 in nerve-injured motor neurons.2005

    • Author(s)
      Egami Y, et al.
    • Journal Title

      Biochem Biophys Res Commun 337

      Pages: 1206-1213

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] The p53-independent nuclear translocation of Cyclin G1 in degenerating neurons by ischemic and traumatic insults.2005

    • Author(s)
      Maeda M, et al.
    • Journal Title

      Exp Neurol 193(2)

      Pages: 350-360

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Noxa is a critical mediator of p53-dependent motor neuron death after nerve injury in adult mouse.2005

    • Author(s)
      Kiryu-Seo S, et al.
    • Journal Title

      JNeurosci 25(6)

      Pages: 1442-1447

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary

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Published: 2005-04-01   Modified: 2016-04-21  

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