| Budget Amount *help |
¥15,100,000 (Direct Cost: ¥15,100,000)
Fiscal Year 2006: ¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 2005: ¥12,800,000 (Direct Cost: ¥12,800,000)
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| Research Abstract |
Next target organs and tissues of tissue engineering and regenerative medicine are the complicated tissues and organs, in which multiple types of cells and various extracellular matrices are arranged at the respective spatial positions at the resolution of micrometer orders. To construct such complicated tissues artificially from cells and biological materials, we need some effective techniques to control microscopic arrangement and compositions of cells and biomaterials. Then, we suggested an innovative approach of tissue engineering, On-demand direct tissue engineering, in which biological tissues are directly reconstructed by on demand ejection of cells and biomaterials at their respective spatial positions. In this research project, we proposed this new approach and investigated its feasibility in tissue engineering. First of all, we needed some substantial technology and machines to examine this approach. Then, we applied on demand inkjet technology and examined to eject living ce
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lls and biomaterials, controlling their spatial positions, and tried layer by layer printing..
We examined living cell printing and confirmed that living cells could be ejected safely by inkjet, and that there were no harmful effects to cell proliferation ability. Next, we invented inkjet gel formation technique to construct three dimensional structures with biological materials. With this technique, we successfully made hydrogel fibers and sheets, and succeeded layer by layer printing to fabricate three dimensional structures. By directly ejecting gel precursor with living cells, we also succeeded direct cell patterning and direct fabrication of those structures containing living cells. In addition, we developed custom made inkjet 3D bioprinter and tried to fabricate several 3D structures containing living cells. Then, we finally succeeded to fabricate three dimensional structures arranged with two different types of cells. The feasibility of On-demand direct tissue engineering was demonstrated. A new phase of tissue engineering is beginning with engineering and technology. Less
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