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Study on the angiogenesis-mediated promotion of bone fracture healing using repeated stimuli induced by microbubble destruction

Research Project

Project/Area Number 17300152
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Biomedical engineering/Biological material science
Research InstitutionOsaka University

Principal Investigator

MATSUMOTO Takeshi  Osaka University, Graduate School of Engineering Science, Associate Professor (30249560)

Co-Investigator(Kenkyū-buntansha) TANAKA Masao  Osaka University, Graduate School ofEngineering Science, Professor (40163571)
OGASAWARA Yasuo  Kawasaki Medical School, 医学部, Associate Professor (10152365)
SONE Teruki  Kawasaki Medical School, 医学部, Associate Professor (90179383)
UESUGI Kentaro  Japan Synchrotron Radiation Research Institute, 放射光実験促進部門, Research & Utilization Division,Researcher (80344399)
NAITO Hisashi  Osaka University, Graduate School of Engineering, Assistant Professor (40392203)
Project Period (FY) 2005 – 2007
Project Status Completed (Fiscal Year 2007)
Budget Amount *help
¥15,180,000 (Direct Cost: ¥14,400,000、Indirect Cost: ¥780,000)
Fiscal Year 2007: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2006: ¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 2005: ¥9,900,000 (Direct Cost: ¥9,900,000)
KeywordsBone regeneration / angiogenesis / in-vivo CT / Image registration / Microbubble / 骨修復 / 放射光骨 / 血管CT / 超音波 / 放射光CT / 皮質骨脈管孔ネットワーク / 骨微小循環
Research Abstract

Bone fracture may trigger elderly people to have osteoporosis, become bedridden, or require nursing care, and furthermore, disturb the bone growth of growing children and increase osteoporosis reserves in the future. To evade these issues, it is required to hasten bone fracture healing and prevent bone atrophy due to the long-term rest, allowing early postoperative ambulation and rehabilitation. Thus, this study was undertaken to clarify the effect of angiogenesis, which promoted by microbubble destruction, on bone fracture healing.
In the 1st year, we performed the pilot study based on μCT to observe the process of bone regeneration and angiogenesis within the bone deficit in rat cortical bone. Woven bone and angiogenesis appeared one week later the deficit operation and its adjacent medullary region. Another week later, the woven bone in the medullary region disappeared with blood vessels, while the woven bone in the cortical bone region remained with sparsely-distributed blood vessel … More s. That is, angiogenesis participated only in the early period of bone healing process. In the 2nd year, we established an in vivo μCT technique to measure bone healing process in the mouse tibia and developed the software to analyze the temporal changes of bone morphometry. On the basis of the in vivo μCT measurement, we could confirm the generation of woven bone and the replacement of it with new bone from the circumference toward the center. As for the last year, to examine the effect of microbubble circulation on bone fracture healing, we performed a μCT measurement of rat tibial bone deficit under microbubble perfusion. No effect of microbubble was found on bone fracture healing. Subsequently we developed an ultrasonic wave generation system. Then, considering a half-time period of spontaneous decay of bubbles and a circulatory time for bubbles to reach the bone deficit region after administration, we examined the timing and the duration to load sound pressure. Presently, we are evaluating the promotive effect of microbubble destruction on angiogenesis and bone regeneration. Less

Report

(4 results)
  • 2007 Annual Research Report   Final Research Report Summary
  • 2006 Annual Research Report
  • 2005 Annual Research Report
  • Research Products

    (40 results)

All 2008 2007 2006 2005

All Journal Article (23 results) (of which Peer Reviewed: 7 results) Presentation (14 results) Book (3 results)

  • [Journal Article] 骨組織における局所材料特性と力学機能2008

    • Author(s)
      松本健志
    • Journal Title

      生体医工学 46

      Pages: 25-30

    • NAID

      110006649731

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Numerical Study on Flows of Red Blood Cells with Liposome-Encapsulated Hemoglobin at Microvascular Bifurcation2008

    • Author(s)
      T. Hyakutake
    • Journal Title

      ASME J. Biomech. Eng. 130

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Numerical Simulations of Circular Particles in Parallel-plate Channel Flow Using Lattice Boltzmann Method2008

    • Author(s)
      T. Hyakutake
    • Journal Title

      J. Comp. Sci. Tech. 2

      Pages: 56-67

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Numerical Study on Flows of Red Blood Cells with Liposome-Encapsulated Hemoglobin at Microvascular Bifurcation2008

    • Author(s)
      T., Hyakutake
    • Journal Title

      ASME J. Biomech. Eng 130

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Numerical Simulations of Circular Particles in Parallel-plate Channel Flow Using Lattice Boltzmann Method2008

    • Author(s)
      T., Hyakutake
    • Journal Title

      J. Comp. Sci. Tech 2

      Pages: 56-67

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Numerical Study on Flows of Red Blood Cells with Liposome-Encapsulated Hemoglobin at Microvascular Bifurcation2008

    • Author(s)
      T Hyakutake.
    • Journal Title

      ASME J Biomech Eng 130(in press)

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 骨組織における局所材料特性と力学機能2008

    • Author(s)
      松本健志, 他
    • Journal Title

      生体医工学 46(in press)

    • NAID

      110006649731

    • Related Report
      2007 Annual Research Report
  • [Journal Article] Fine Structure of Porous Canal Network in Cortical Bone2007

    • Author(s)
      T. Matsumoto
    • Journal Title

      Research Frontiers

      Pages: 47-48

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Microheterogeneity of Regional Myocardial Blood Flows in Low-Perfused Rat Hearts Evaluated by Double-Tracer Digital Radiography2007

    • Author(s)
      T. Matsumoto
    • Journal Title

      Appl. Radiat. Isotopes 65

      Pages: 910-917

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Biphasic Change and Disuse-Mediated Regression of Canal Network Structure in Cortical Bone of Growing Rats2007

    • Author(s)
      T. Matsumoto
    • Journal Title

      Bone 41

      Pages: 239-246

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Fine Structure of Porous Canal Network in Cortical Bone2007

    • Author(s)
      T., Matsumoto
    • Journal Title

      SPring-8 Research Frontiers 2006

      Pages: 47-48

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Microheterogeneity of Regional Myocardial Blood Flows in Low-Perfused Rat Hearts Evaluated by Double-Tracer Digital Radiography2007

    • Author(s)
      T., Matsumoto
    • Journal Title

      Appl. Radiat. Isotopes 65

      Pages: 910-917

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Biphasic Change and Disuse-Mediated Regression of Canal Network Structure in Cortical Bone of Growing Rats2007

    • Author(s)
      T., Matsumoto
    • Journal Title

      Bone 41

      Pages: 239-246

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Biphasic Change and Disuse-Mediated Regression of Canal Network Structure in Cortical Bone of Growing Rats2007

    • Author(s)
      T Matsumoto.
    • Journal Title

      Bone 41

      Pages: 239-246

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Fine structure of porous canal network in cortical bone2007

    • Author(s)
      T Matsumoto, et al.
    • Journal Title

      SPring-8 Research Frontiers 2007(in press)

    • Related Report
      2006 Annual Research Report
  • [Journal Article] 放射光CTによる皮質骨微細形態解2007

    • Author(s)
      松本健志, 他
    • Journal Title

      生体医工学 44(4)(in press)

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Monochromatic Synchrotron Radiation μCT Reveals Disuse-Mediated Canal Network Rarefaction in Cortical Bone of Growing Rat Tibiae2006

    • Author(s)
      T. Matsumoto
    • Journal Title

      J. Appl. Physiol. 100

      Pages: 274-280

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] 放射光CTによる皮質骨微細形態解析2006

    • Author(s)
      松本健志
    • Journal Title

      生体医工学 44

      Pages: 517-521

    • NAID

      110006249807

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Lattice Boltzmann Simulation of Blood Cell Behavior at Microvascular Bifurcations2006

    • Author(s)
      T. Hyakutake
    • Journal Title

      Math. Comput. Simulat. 72

      Pages: 134-140

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Monochromatic Synchrotron Radiation mCT Reveals Disuse-Mediated Canal Network Rarefaction in Cortical Bone of Growing Rat Tibiae2006

    • Author(s)
      T., Matsumoto
    • Journal Title

      J. Appl. Physiol 100

      Pages: 274-280

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Lattice Boltzmann Simulation of Blood Cell Behavior at Microvascular Bifurcations2006

    • Author(s)
      T., Hyakutake
    • Journal Title

      Math. Comput. Simulat 72

      Pages: 134-140

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Lattice Boltzmann simulation of blood cell behavior at microvascular bifurcations2006

    • Author(s)
      T Hyakutake, et al.
    • Journal Title

      Mathematics and Computers in Simulation 72(2-6)

      Pages: 134-140

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Monochromatic synchrotron radiation μCT reveals disuse-mediated canal network rarefaction in cortical bone of growing rat tibiae2006

    • Author(s)
      T Matsumoto, et al.
    • Journal Title

      Journal of Applied Physiology 100・1

      Pages: 274-280

    • Related Report
      2005 Annual Research Report
  • [Presentation] Trabecular Bone Dynamics in Mice Subjected to Unilateral Sciatic Neurectomy Assessed by in vivo μCT Using Monochromatic Synchrotron Radiation2008

    • Author(s)
      T. Matsumoto
    • Organizer
      Exp Biol.
    • Place of Presentation
      San Diego
    • Year and Date
      2008-04-07
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Trabecular Bone Dynamics in Mice Subjected to Unilateral Sciatic Neurectomy Assessed by in vivo μCT Using Monochromatic Synchrotron Radiation2008

    • Author(s)
      T., Matsumoto
    • Organizer
      Exp Biol
    • Place of Presentation
      San Diego
    • Year and Date
      2008-04-07
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Transport Model of Physiological Substances in Cortical Bone through Porous Canal Network2007

    • Author(s)
      T Kometa, et. al.
    • Organizer
      Proc 3rd Asian Pacific Conf Biomech
    • Place of Presentation
      Tokyo
    • Year and Date
      2007-11-06
    • Related Report
      2007 Annual Research Report
  • [Presentation] Transport Model of Physiological Substances in Cortical Bone through Porous Canal Network2007

    • Author(s)
      T .Komeda
    • Organizer
      3rd Asian Pacific Conf. Biomech.
    • Place of Presentation
      Tokyo
    • Year and Date
      2007-11-05
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Transport Model of Physiological Substances in Cortical Bone through Porous Canal Network2007

    • Author(s)
      T., Komeda
    • Organizer
      3rd Asian Pacific Conf Biomech
    • Place of Presentation
      Tokyo
    • Year and Date
      2007-11-05
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Time Response of the Canal Network in Cortical Bone to Sciatic Neurectomy in Growing Rats: a Synchrotron Radiation μCT Study2006

    • Author(s)
      T. Matsumoto
    • Organizer
      5th World Cong. Biomech.
    • Place of Presentation
      Munich
    • Year and Date
      2006-08-02
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Time Response of the Canal Network in Cortical Bone to Sciatic Neurectomy in Growing Rats : a Synchrotron Radiation μCT Study2006

    • Author(s)
      T., Matsumoto
    • Organizer
      5th World Cong. Biomech
    • Place of Presentation
      Munich
    • Year and Date
      2006-08-02
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Reduced Myocardial Flow Heterogeneity Under Exchange Transfusion With Liposomal Hemoglobin Explained by a Branching Tree Model With Effective Blood Viscosity and Red Cell Distribution2006

    • Author(s)
      T. Matsumoto
    • Organizer
      5th World Cong. Biomech.
    • Place of Presentation
      Munich
    • Year and Date
      2006-07-31
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Reduced Myocardial Flow Heterogeneity Under Exchange Transfusion With Liposomal Hemoglobin Explained by a Branching Tree Model With Effective Blood Viscosity and Red Cell Distribution2006

    • Author(s)
      T., Matsumoto
    • Organizer
      5th World Cong. Biomech
    • Place of Presentation
      Munich
    • Year and Date
      2006-07-31
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Effects of Sciatic Neurectomy on Cortical Bone Microstructure in Juvenile Rats: Threee-Dimensional Appearance of Canal Networkevaluated by Synchrotron Radiation Microtomography2005

    • Author(s)
      M. Yoshino
    • Organizer
      12th Int. Conf. Biomed. Eng.
    • Place of Presentation
      Singapore
    • Year and Date
      2005-12-09
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Effects of Sciatic Neurectomy on Cortical Bone Microstructure in Juvenile Rats : Threee-Dimensional Appearance of Canal Networkevaluated by Synchrotron Radiation Microtomography2005

    • Author(s)
      M., Yoshino
    • Organizer
      12th Int. Conf. Biomed. Eng
    • Place of Presentation
      Singapore
    • Year and Date
      2005-12-09
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Synchrotron Radiation Microtomography Reveals Disuse Effects on Cortical Canal Network Structure2005

    • Author(s)
      T. Matsumoto
    • Organizer
      2nd Japan-Switzerland Workshop Biomech.
    • Place of Presentation
      Kyoto
    • Year and Date
      2005-09-15
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Synchrotron Radiation Microtomography Reveals Disuse Effects on Cortical Canal Network Structure2005

    • Author(s)
      T., Matsumoto
    • Organizer
      2nd Japan-Switzerland Workshop Biomech
    • Place of Presentation
      Kyoto
    • Year and Date
      2005-09-15
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Effects of Sciatic Neurectomy on Canal Network Structure in Juvenile Cortical Bone2005

    • Author(s)
      M., Yoshino
    • Organizer
      6th Asian-Pacific Conf. Med. Biol. Eng
    • Place of Presentation
      Tsukuba
    • Year and Date
      2005-04-25
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Book] Microscopic Analysis of Bone(in H. Wada(ed.): Biomechanics at Micro-and Nanoscale Levels IV2007

    • Author(s)
      M. Tanaka
    • Publisher
      World Scientific
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Book] Assessment of Cortical Bone Microstructure Using Monochromatic Synchrotron Radiation Micro-CT(in H. Wada(ed.): Biomechanics at Micro-and Nanoscale Levels II2006

    • Author(s)
      T. Matsumoto
    • Publisher
      World Scientific
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Book] Assessment of cortical bone microstructure using monochromatic synchrotron radiation micro-CT. In : Biomechanics at micro- and nanoscale levels, vol.2(Wada H(ed))2006

    • Author(s)
      T Matsumoto, et al.
    • Publisher
      World Scientific(in press)
    • Related Report
      2005 Annual Research Report

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Published: 2005-04-01   Modified: 2016-04-21  

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