| Project/Area Number |
17300163
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biomedical engineering/Biological material science
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| Research Institution | Kansai University |
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Principal Investigator |
OUCHI Tatsuro Kansai University, Faculty of Chemistry, Materials and Bioengineering, Professor (60067650)
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| Co-Investigator(Kenkyū-buntansha) |
OHYA Yuichi Kansai University, Faculty of Chemistry, Materials and Bioengineering, Professor (10213886)
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| Project Period (FY) |
2005 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥15,720,000 (Direct Cost: ¥14,700,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2007: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2006: ¥3,700,000 (Direct Cost: ¥3,700,000)
Fiscal Year 2005: ¥7,600,000 (Direct Cost: ¥7,600,000)
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| Keywords | Polylactide / Multi-Arm / Poly(ethylene glycol) / Branched Block Copolymer / Physical Property / Biodegrdable / Temperature-resposiveness / Hydrogel / 分岐プロック共重合体 / 吸水性 / ソフト医用材料 |
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| Research Abstract |
To explore the potential of a star-shaped 8-arms poly(ethylene glycol)35K-b-poly(L-lactide)37K (8-arms PEG35K-b-PLLA37K: M_n of PEG=35,000, M_n of PLLA=37,000) film as a novel bioabsorbable adhesion-prevention membrane, water structure, surface contact angle, protein adsorption, and cell and platelet anti-adhesion properties of such a hydrated film were investigated. Based on these results, it was found that the 8-arms PEG35K-b-PLLA37K film exhibited a biologically inert surface, which was the result of a large number of PEG chains and a free water layer on the film surface. This led to a reduction in protein absorption and cell and platelet adhesion onto the film surface. This implies the star-shaped 8-arms PEG35K-b-PLLA37K film can be utilized as a novel bioabsorbable adhesion-prevention membrane.
Partially cholesterol-substituted 8-arm poly(ethylene glycol)-block-poly(L-lactide) (8-arm PEG-b-PLLA-cholesterol) was prepared as a novel star-shaped, biodegradable copolymer derivative. The amphiphilic 8-arm PEG-b-PLLA-cholesterol aqueous solution (polymer concentration, above 3 wt%) exhibited instantaneous temperature-induced gelation at 34℃, but the virgin 8-arm PEG-b-PLLA did not, irrespective of concentration. Moreover, we successfully created an extracellular matrix (ECM)-like micrometer-scale network structure with favorable porosity for 3D proliferation of cells inside the hydrogel. This network structure was mainly attributed to specific self-assembly between cholesterol groups. The 10 and 20 wt% hydrogels were eroded gradually in PBS at 37℃ over the course of a month, and the gel became completely dissociated. Moreover, the L929 cells encapsulated into the hydrogel were viable and proliferated 3-dimensionally inside the hydrogels as a result of the ECM-like network structure. Thus, in vitro cell culture studies demonstrated that 8-arm PEG-b-PLLA-cholesterol is a promising candidate as a novel injectable cell scaffold.
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