| Project/Area Number |
17300187
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Rehabilitation science/Welfare engineering
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| Research Institution | Himeji Dokkyo University (2006-2007)
Suzuka University of Medical Science (2005) |
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Principal Investigator |
FUJINO Hidemi Himeji Dokkyo University, Department of Physical Therapy, Professor (20278998)
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| Co-Investigator(Kenkyū-buntansha) |
ISHIHARA Akihiko Kyoto University, Graduate School of Human and Environmental Studies, Professor (90184548)
TAKEDA Isao Himeji Dokkyo University, Department of Physical Therapy, Professor (00163402)
KAJIYA Fumihiko Kawasaki University of Medical Welfare, Department of Medical Engineering, Professor (70029114)
上月 久治 姫路獨協大学, 医療保健学部, 教授 (20175326)
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| Project Period (FY) |
2005 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥13,640,000 (Direct Cost: ¥13,100,000、Indirect Cost: ¥540,000)
Fiscal Year 2007: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2006: ¥4,100,000 (Direct Cost: ¥4,100,000)
Fiscal Year 2005: ¥7,200,000 (Direct Cost: ¥7,200,000)
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| Keywords | rehabilitation / proteome / molecular signaling / teprenon / molecular chaperones / heat shock protei / skeletal muscle / disuse atrophy |
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| Research Abstract |
Geranylgeranylacetone (GGA) has been used in clinics for many years as a therapeutic agent for gastric ulcer and gastritis. GGA induces 70-kDa heat shock protein (HSP70) selectively, and HSPs are induced as an adaptative response in all cells and organisms upon exposure to a number of stresses. HSPs function as molecular chaperones involved in the correct folding and refolding of proteins, and thereby exert essential housekeeping and protective functions. In this study, we thus investigated the effects of GGA on skeletal muscle under normal and stressful conditions such as disuse atrophy by hindlimb unloading in rats. Skeletal muscles were isolated 24 hours after administration of GGA at 0,200,400 and 600 mg/kg. In soleus, gastrocnemius, plantaris and extensor digitorum longus muscles, increasing the dose up to 600 mg/kg did not result in a further increase in HSP72 expression. GGA primed other types of cells for enhanced induction of HSP70, when exposed to stress. In addition, we investigated the induction of HSP70 after administration of GGA in hindlimb unloading condition. The HSP72 levels decreased in the atrophied muscle compare with control, whereas these values in the GGA administrated muscles attenuated the decrease of HSP70 in hindlimb unloading condition. Although hindlimb unloading resulted in muscle atrophy, the loss of myofibrillar protein content was significantly less in the GGA administrated skeletal muscle. These results suggest that GGA may exhibit protective actions independently of Hsp70 induction. Additionally, these observations may also indicate that the administration of GGA could be one of the useful tools to attenuate muscle atrophy.
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