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The response of adipose tissues to oxidative stress by exercise : with special reference to the role of EC-SOD in splanchnic adioise tissue

Research Project

Project/Area Number 17300211
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Sports science
Research InstitutionKyorin University

Principal Investigator

OHNO Hideki  Kyorin University, School of Medicine, Professor, 医学部, 教授 (00133819)

Co-Investigator(Kenkyū-buntansha) KIZAKI Takako  Kyorin University, School of Medicine, Associate Professor, 医学部, 助教授 (00322446)
NAKANO Norihiko  Kyorin University, School of Medicine, Associate Professor, 医学部, 講師 (40322721)
SAKURAI Takuya  Kyorin University, School of Medicine, Instructor, 医学部, 助手 (20353477)
Project Period (FY) 2005 – 2006
Project Status Completed (Fiscal Year 2006)
Budget Amount *help
¥15,300,000 (Direct Cost: ¥15,300,000)
Fiscal Year 2006: ¥4,900,000 (Direct Cost: ¥4,900,000)
Fiscal Year 2005: ¥10,400,000 (Direct Cost: ¥10,400,000)
Keywordsexercise / oxidative stress / splanchnic adipose tissue / peripheral adipose tissue / extracellular superoxide dismutase / adipokine / metabolic syndrome / polyphenol / 白色脂肪組織 / 褐色脂肪組織 / EC-SOD / 脱共役タンパク質2 / β-アレスチン / 脱共役たんぱく質2
Research Abstract

The main findings obtained during 2005-2006 are as follows:
1. The effect of acute exercise on β-adrenergic receptor (β-AR) trafficking was investigated in rat adipocytes. As the result, the mechanism underlying the increased density of β_2-AR up to at least 3 h after exercise might involve alterations in a multistep event involving the coordinate interaction among proteins mediating β_2-AR trafficking, in which both the receptor-agonist interactions and ubiquitin-proteasome pathway have a key role. However, the decreased protein expression of β_2-AR at 24 h might be due to some change occurring at the translational levels.
2. The effect of exercise training on oxidative stress was investigated in rat adipocytes. In consequence, the level of thiobarbituric acid-reactive substances (TBARS) was significantly decreased in splanchnic adipose tissue, but not in peripheral adipose tissue. Meanwhile, extracellular superoxide dismutase (EC-SOD) level appeared to increase only in splanchnic adipo … More se tissue. Considering reduced level of monocyte chemoattractant protein-1 (MCP-1) in splanchnic adipose tissue after training, the findings obtained suggest that physical training reduces oxidative stress in splanchnic adipose tissue, resulting in improvement in lifestyle-related disease.
3. The antioxidative effect of new lychee-derived polyphenol Oligonol converted into a low-molecular form (Amino Up Chemical Co., Ltd., Sapporo) was investigated in mouse adipocytes. Although the levels of splanchnic adipose tissue mass and TBARS in mice given a high fat diet (HFD) were significantly higher than those in control mice, Oligonol definitely inhibited such HFD-induced increases. Expression of TNF-α and MCP-1 genes in splanchnic adipose tissue of HFD mice was also significantly higher than that in control and HFD + Oligonol mice. Moreover, the expression of EC-SOD gene was enhanced only in splanchnic adipose tissue of HFD + Oligonol mice. These results suggest that Oligonol has favorable effects on oxidative stress. Less

Report

(3 results)
  • 2006 Annual Research Report   Final Research Report Summary
  • 2005 Annual Research Report
  • Research Products

    (36 results)

All 2007 2006 2005 Other

All Journal Article (34 results) Book (2 results)

  • [Journal Article] High altitude and oxidative stress2007

    • Author(s)
      Dosek, A. et al.
    • Journal Title

      Respir. Physiol. Neurobiol. (in press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Exercise before or after refeeding prevents refeeding-induced recovery of cell size after fasting with a different pattern of metabolic gene expressions in rat epididymal adipocytes2007

    • Author(s)
      Sakurai, T. et al.
    • Journal Title

      Metabolism (in press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Disposition of protein-bound 3-nitrotyrosine in rat plasma analyzed by a novel protocol for HPLC-ECD2007

    • Author(s)
      Hitomi, Y. et al.
    • Journal Title

      J. Biochem. 141・4

      Pages: 495-502

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] SHP-2 positively regulates adipogenic differentiation in 3T3-L1 cells2007

    • Author(s)
      Uehara, T. et al.
    • Journal Title

      Int. J. Mol. Med. 19・6

      Pages: 885-900

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Transcription regulation of gene expression in rat brown adipose tissue in response to unloading or 2G loading during growing period.2007

    • Author(s)
      Watanabe, S., et al.
    • Journal Title

      Acta Astronaut. 60

      Pages: 889-898

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Disposition of protein-bound 3-nitrotyrosine in rat plasma analyzed by a novel protocol for HPLC-ECD.2007

    • Author(s)
      Hitomi, Y., et al.
    • Journal Title

      J. Biochem. 141・4

      Pages: 495-502

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] SHP-2 positively regulates adipogenic differentiation in 3T3-L1 cells.2007

    • Author(s)
      Uehara, T., et al.
    • Journal Title

      Int. J. Mol. Med. 19・6

      Pages: 895-900

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] SHP-2 positively regulates adipogenic differentiation in 3T3-L1 cells2007

    • Author(s)
      Uehara, T. et al.
    • Journal Title

      Int. J. Mol. Med. in press

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Exercise before or after refeeding prevents refeeding-induced recovery of cell size after fasting with a different pattern of metabolic gene expressions in rat epididymal adipocytes2007

    • Author(s)
      Sakurai, T. et al.
    • Journal Title

      Metabolism in press

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Disposition of protein-bound 3-nitrotyrosine in rat plasma analyzed by a novel protocol for HPLC-ECD2007

    • Author(s)
      Hitomi, Y. et al.
    • Journal Title

      J. Biochem. in press

    • Related Report
      2006 Annual Research Report
  • [Journal Article] High altitude and oxidative stress2007

    • Author(s)
      Dosek, A. et al.
    • Journal Title

      Respir. Physiol. Neurobiol. in press

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Role (s) of gravitational loading on the transcription regulation of gene expression in rat brown adipose tissue during growing period2007

    • Author(s)
      Ohira, Y. et al.
    • Journal Title

      J. Gravitat. in press

    • Related Report
      2006 Annual Research Report
  • [Journal Article] β- Adrenergic receptor trafficking by exercise in rat adipocytes : roles of G- protein- coupled receptor kinase- 2, β - arrestin -2, and the ubiquitin - proteasome pathway2006

    • Author(s)
      Ogasawara, J. et al.
    • Journal Title

      FASEB J. 20・2

      Pages: 350-352

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Muscle type-specific response of PGC-1 α and oxidative enzymes during voluntary wheel running in mouse skeletal muscle2006

    • Author(s)
      Ikeda, S. et al.
    • Journal Title

      Acta Physiol. 188・3-4

      Pages: 217-223

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] β-Adrenergic receptor trafficking by exercise in rat adipocytes : roles of G-protein-coupled receptor kinase-2, β-arrestin-2, and the ubiquitin- proteasome pathway.2006

    • Author(s)
      Ogasawara, J., et al.
    • Journal Title

      FASEB J. 20・2

      Pages: 350-352

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Muscle type-specific response of PGC-1 α and oxidative enzymes during voluntary wheel running in mouse skeletal muscle.2006

    • Author(s)
      Ikeda, S., et al.
    • Journal Title

      Acta Physiol 188・3-4

      Pages: 217-223

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Disturbance of microcirculation due to unhealthy lifestyle : Cause of type 2 diabetes.2006

    • Author(s)
      Matsumoto, Y., et al.
    • Journal Title

      Med. Hypotheses 66・3

      Pages: 550-553

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Fatal hyperthermia due to dental treatment.2006

    • Author(s)
      Noguchi, I., et al.
    • Journal Title

      Oral Surg. Oral Pathol. Oral Radiol. Endod. 101・3

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Effects of acute exercise on lung antioxidant enzymes in young and old rats.2006

    • Author(s)
      Hatao, H., et al.
    • Journal Title

      Mech. Ageing Dev. 127・4

      Pages: 384-390

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Gliclazide protects 3T3L1 adipocytes against insulin resistance induced by hydrogen peroxide with restoration of GLUT4 translocation.2006

    • Author(s)
      Shimoyama, T., et al.
    • Journal Title

      Metabolism 55・6

      Pages: 722-730

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Calcineurin mediated slow-type fiber expression and growth in reloading condition.2006

    • Author(s)
      Miyazaki, M., et al.
    • Journal Title

      Med. Sci. Sports Exerc. 38・6

      Pages: 1065-1072

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Muscle type-specific response of PGC-1 α and oxidative enzymes during voluntary wheel running in mouse skeletal. muscle2006

    • Author(s)
      Ikeda, S. et al.
    • Journal Title

      Acta Physiol. 188・3-4

      Pages: 217-223

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Gliclazide protects 3T3L1 adipocytes against insulin resistance induced by hydrogen peroxide with restoration of GLUT4 translocation2006

    • Author(s)
      Shimoyama, T. et al.
    • Journal Title

      Metabolism (in press)

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Calcineurin mediated slow-type fiber expression and growth in reloading condition2006

    • Author(s)
      Miyazaki, M. et al.
    • Journal Title

      Med. Sci. Sports Exerc. (in press)

    • Related Report
      2005 Annual Research Report
  • [Journal Article] β-Adrenergic receptor trafficking by exercise in rat adipocytes : roles of G-protein-coupled receptor kinase-2, β-arrestin-2, and the ubiquitin-proteasome pathway2006

    • Author(s)
      Ogasawara, J. et al.
    • Journal Title

      FASEB J. (in press)

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Effects of acute exercise on lung antioxidant enzymes in young and old rats2006

    • Author(s)
      Hatao, H. et al.
    • Journal Title

      Mech. Ageing Dev. (in press)

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Transcription regulation of gene expression in rat brown adipose tissue in response to unloading or 2-G loading during the rapid growth phase2006

    • Author(s)
      Watanabe, S. et al.
    • Journal Title

      Acta Astronaut. (in press)

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Disturbance of microcirculation due to unhealthy lifestyle : Cause of type 2 diabetes2006

    • Author(s)
      Matsumoto, Y. et al.
    • Journal Title

      Med. Hypotheses 66・3

      Pages: 550-553

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Changes in nitric oxide and inducible nitric oxide synthase following stretch-induced injury to the tibialis anterior muscle of rabbit.2005

    • Author(s)
      Sakurai, T., et al.
    • Journal Title

      Jpn. J. Physiol. 55・2

      Pages: 101-107

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Exercise training enhances tumor necrosis factor-α-induced expressions of anti-apoptotic genes without alterations in caspase-3 activity in rat epididymal adipocytes.2005

    • Author(s)
      Sakurai, T., et al.
    • Journal Title

      Jpn. J. Physiol. 55・3

      Pages: 181-189

    • NAID

      10016882495

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] We have two strategies to attain healthy ageing.2005

    • Author(s)
      Ohno, H., et al.
    • Journal Title

      Geriatr. Gerontol. Int. 4

    • NAID

      10018027970

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Is angiotensin I-converting enzyme I/D polymorphism associated with endurance performance and or high altitude adaptation ?2005

    • Author(s)
      Ohno, H., et al.
    • Journal Title

      Adv. Exerc. Sports Physiol. 11・2

      Pages: 41-54

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Exercise before or after refeeding prevents refeeding-induced recovery of cell size after fasting with a different pattern of metabolic gene expressions in rat epididymal adipocytes.

    • Author(s)
      Sakurai, T., et al.
    • Journal Title

      Metabolism (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] High altitude and oxidative stress.

    • Author(s)
      Dosek, A., et al.
    • Journal Title

      Respir. Physiol. Neurobiol. (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Book] 体力とはなにか-運動処方のその前に-2007

    • Author(s)
      大野秀樹・他
    • Total Pages
      237
    • Publisher
      ナップ
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Annual Research Report 2006 Final Research Report Summary
  • [Book] やさしい生理学改訂第5版2005

    • Author(s)
      大野 秀樹
    • Total Pages
      297
    • Publisher
      南江堂
    • Related Report
      2005 Annual Research Report

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Published: 2005-04-01   Modified: 2016-04-21  

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