| Project/Area Number |
17310031
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Risk sciences of radiation/Chemicals
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| Research Institution | Saitama University |
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Principal Investigator |
WANG Qingyue Saitama University, Graduate School of Science & Engineering, Associate Professor (30344956)
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| Co-Investigator(Kenkyū-buntansha) |
UCHIYAMA Iwao Kyoto University, Graduate School of Engineering, Professor (20151897)
MIWA Makoto Center for Environmental Science in Saitama, 埼玉県環境科学国際センター, Researcher (30375589)
SAKAMOTO Kazuhiko Saitama University, Graduate School of Science & Engineering, Professor (20111411)
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| Project Period (FY) |
2005 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥16,790,000 (Direct Cost: ¥15,500,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2007: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2006: ¥5,700,000 (Direct Cost: ¥5,700,000)
Fiscal Year 2005: ¥5,500,000 (Direct Cost: ¥5,500,000)
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| Keywords | Japanese cedar pollen / Urban area / Air pollutants / Allergen particles / Size distribution / Collection of air samples / Analytical methods / アレルゲン / 微小化 / 抽出法 / 花粉 / 寄与率 |
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| Research Abstract |
The purpose of this study is to evaluate the adverse factors influencing by air pollutants and to aid biomedical research by investigating the deposition of air pollutants on Japanese cedar pollen between urban and mountainous areas. Suspended particulate matter and Japanese cedar perigonium pollen were collected to identify the deposition of air pollutants at Saitama urban area and a mountainous area, during the pollination season. Ionic and metallic components of these samples and perigonium and pollen were analyzed. We found that crustal components (Al, Fe and Ti) tended to deposit on perigonium pollen. The amount of ionic components of particles (NO_3^-, SO_4^<2-> and NH_4^+) and gaseous pollutants (NO_2, SO_2 and NH_3) deposited on the perigonium pollen were higher in the urban than in the mountainous areas, the fact of which was proven by determination of gaseous pollutants exposed to Japanese cedar pollen. It is suggested that these phenomena may affect pollinosis and therefore
… More
it is necessary to investigate implication on biomedical researches.
In addition, morphological observation of Ubisch bodies exfoliated from pollen and ruptured pollens was performed and confirmed with a scanning electron microscope. Moreover, the existing evidences of visualized Cry j 1 and Cry j 2 allergen particles below 1.1 μm were also sensitively found by an immunofluorescence technique with a fluorescence microscope. Simultaneously, the high concentrations of Cry j 1 and/or Cry j 2 were determined in the fine particle sizes below 1.1 μm. It was suggested that airborne respirable particulate matters containing Cry j 1 and Cry j 2 play a key role in the increasing incidence of asthma in the lower respiratory tract. Furthermore, since Cry j 1 (or Cry j 2) fine particles were suspended in the atmosphere over a long time more after released, the daily variation delays in the peaks of these concentrations compared with the peaks of airborne pollen counts were observed. It is important to observe and determine the size distribution of airborne Japanese cedar pollen allergen particles in the long-term sampling research continually, and to elucidate the allergen particle release mechanisms. Less
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