| Co-Investigator(Kenkyū-buntansha) |
NASU Tamie Nagoya University, Graduate School of Medicine, Professor (10020794)
TAKAGI Kenji Nagoya University, School of Medicine, Associate professor (80126870)
SHIBATA Eiji Aichi Medical University, School of Medicine, Associate professor (90206128)
YAMONO Yuko Showa University, School of Medicine, Assistant professor (30167580)
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| Budget Amount *help |
¥16,250,000 (Direct Cost: ¥15,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2007: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2006: ¥2,500,000 (Direct Cost: ¥2,500,000)
Fiscal Year 2005: ¥10,500,000 (Direct Cost: ¥10,500,000)
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| Research Abstract |
This study aims at clarifying dose-response relationship between insecticide urinary metabolites and health effects, which can he used for risk assessment of simultaneous exposure to several pesticides. Highly sensitive analytical methods for dialkyl phosphates (DAPs), metabolites of organophosphorus insecticides, and 3-phenoxybenzoic acid (3-PBA), a metabolite of pyrethroids, were established and applied to evaluate exposure level in the exposed workers DAPs were revealed to positively correlate with urinary 8-hydroxydeoxyguanosine(8-OHdG)levels in the exposed workers. There was no significant association between genotype [Q (glutamine) /Q, Q/R (argnine), R/R] of PON1, an enzyme hydrolizing oxon-type organophosphorus, and leucocyte/urinary 8-OHdG, but DAP concentrations in summer significantly decreased as the number of Q allele decreased. As for 3-PBA, its urinary concentration was significantly different only in winter between sprayers and non-sprayers working in the same companies.
… More
In animal experiments, 40 male Wistar rats at 9 weeks of age were orally treated with dichlorvos (DDVP) 0, 5, or 10 mg/kg, or diazinon(DZN)3 mg/kg 6 days a week for 9 weeks. Rats treated with DZN showed significant decrease in sperm motility, and ATP/ADP ratio in sperm in DDVP 10mg/kg-treated group significantly decreased 60 min after sperm release into the medium. Short-tailed sperm in the DDVP 10mg/kg and-DZN groups significantly increased, and the regression was significant between the urinary DAP concentrations and sperm motility or the incidence of short-tailed sperm. Oral treatment with cis-permethrin (0, 35, or 70 mg/kg) for 42 days in ICR mice induced dose-dependent reduction of sperm count, sperm motility, testicular testosterone production and plasma testosterone concentration. It was suggested that cis-permethrin impaired mitochondrial membrane in Leydig cells and disrupt testosterone biosynthesis. Considering the results found in occupationally exposed populations, risk assessment of insecticide w-exposure needs to be conducted based on the biological monitoring data of the urinary pesticide metabolites. Less
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