Risk evaluation and toxicity mechanism of air-borne particulate matter and its major chemical components

• Project/Area Number: 17310039
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Risk sciences of radiation/Chemicals
• Research Institution: Osaka Prefecture University
• Principal Investigator: YAGI Takashi Osaka Prefecture University, 産学官連携機構, 教授 (80182301)
• Co-Investigator(Kenkyū-buntansha): MIZOHATA Akira 大阪府立大学, 産学官連携機構, 教授 (80090439) ; KAWANISHI Masanobu 大阪府立大学, 産学官連携機構, 助教 (70332963) ; NAKAYAMA Aki 京都大学, 大学院・工学研究科, 助教 (10335200)
• Project Period (FY): 2005 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥17,440,000 (Direct Cost: ¥15,400,000、Indirect Cost: ¥2,040,000); Fiscal Year 2008: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2007: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2006: ¥3,800,000 (Direct Cost: ¥3,800,000); Fiscal Year 2005: ¥4,800,000 (Direct Cost: ¥4,800,000)
• Keywords: 大気汚染 / 粒子状物質 / 環境変異原 / がん / リスク / 発がん性 / 変異原性 / 芳香族炭化水素 / PM2.5 / 変異原生

Research Abstract

堺市で採取した大気浮遊粒子の変異原性および内分泌撹乱性(ダイオキシン受容体およびエストロゲン受容体リガンド活性)を確認した。その主たる成分であるベンゾ(a)ピレン、1, 8-ジニトロピレン、3, 6-ジニトロベンゾ(e)ピレン、3-ニトロベンズアントロン、4-アミノビフェニルの突然変異誘発機構を分子・細胞生物学的方法で調べ、それらに共通した細胞周期特異的なDNA 損傷応答、および損傷に特異的なDNA ポリメラーゼの関与と突然変異誘発性を明らかにした。

Research Products

• [Journal Article] Genotoxicity of 3, 6-dinitrobenzo[e]pyrene, a novel mutagen in ambient air and surface soil, in mammalian cells in vitro and in vivo (2009)
  • Author(s): Kawanishi, M., Watanabe, T., Hagio, S., Ogo, S., Shimohara, C., Jouchi, R., Takayama, S., Hasei, T., Hirayama, T., Oda, Y. and Yagi, T.
  • Journal Title: Mutagenesis (in press)
  • Peer Reviewed
• [Journal Article] Establishment of a method for analyzing translesion DNA synthesis across a single bulky adduct in human cells (2009)
  • Author(s): Sawai, T., Kawanishi, M., Takamura-Enya, T. and Yagi, T.
  • Journal Title: Genes Environ 31(1) Pages: 24-30
  • NAID: 110007138016
  • Peer Reviewed
• [Journal Article] Establishment of a Method for Analyzing Translesion DNA Synthesis across a Single Bulky Adduct in Human Cells. (2009)
  • Author(s): Tomoko Sawail, Masanobu Kawanishi, Takeji Takamura-Enya, Takashi Yagi.
  • Journal Title: Genes and Environment 31 Pages: 24-30
  • NAID: 110007138016
  • Peer Reviewed
• [Journal Article] Polyaromatic hydrocarbons cause histone H2AX phosphorylation in the S-phase of the cell cycle (2008)
  • Author(s): Shimohara, C., Sawai, T. and Yagi, T.
  • Journal Title: Genes Envron 30(3) Pages: 92-99
  • NAID: 110006873846
  • Peer Reviewed
• [Journal Article] Mutagenic specificity of N-acetoxy-3-aminobenzanthrone, a major metabolically activated form of 3-nitrobenzanthrone, in shuttle vector plasmids propagated in human cells (2008)
  • Author(s): Nishida, H., Kawanishi, M., Takamura-Enya, T., Yagi, T.
  • Journal Title: Mutat Res 654 Pages: 82-87
  • Peer Reviewed
• [Journal Article] Polyaromatic Hydrocarbons Cause Histone H2AX Phosphorylation in the S-phase of the Cell Cycle. (2008)
  • Author(s): Chiaki Shimohara, Tomoko Sawai, Takashi Yagi.
  • Journal Title: Genes and Environment. 30 Pages: 92-99
  • NAID: 110006873846
  • Peer Reviewed
• [Journal Article] Mutagenic Specificity of N-Acetoxy-3-Aminobenzanthrone, a Major Metabolically Activated Form of 3-Nitrobenzanthrone, in Shuttle Vector Plasmids Propagated in Human Cells. (2008)
  • Author(s): Hiroshi Nishida, Masanobu Kawanishi, Takeji Takamura-Enya, Takashi Yagi.
  • Journal Title: Mutation Research 654 Pages: 82-88
  • Peer Reviewed
• [Journal Article] Structural identification of DNA adducts derived from 3-Nitrobenzanthrone, a potent carcinogen present in the atmosphere (2007)
  • Author(s): Takamura-Enya, T., Kawanishi, M. Yagi, T.and Hisamatsu, Y.
  • Journal Title: Chem. Asian J 2 Pages: 1174-1185
  • Peer Reviewed
• [Journal Article] DNA adduct formation in human hepatoma cells treated with 3-nitrobenzanthrone : analysis by the ^<32>P-postlabeling method (2007)
  • Author(s): Kanno, T., Kawanishi, M., Takamura-Enya, T., Arlt, V. M., Phillips, D. H.,Yagi. T.
  • Journal Title: Mutat. Res 634 Pages: 184-191
  • Peer Reviewed
• [Journal Article] Fabrication of reference filter for measurement of EC(elemental carbon)and OC(Organic carbon)in aerosol particles (2007)
  • Author(s): Lee, H.M., Okuyama, K., Mizohata, A.,Kim, T.O., Koyama, H.
  • Journal Title: Aerosol Sci. Technol 41 Pages: 281-294
  • Peer Reviewed
• [Journal Article] Structural ldentification of DNA Adducts Derived from 3-Nitrobenzanthrone, a Potent Carcinogen Present in the Atmosphere (2007)
  • Author(s): Takamura-Enya, T., Kawanishi, M., Yagi, T. and Hisamatsu, Y.
  • Journal Title: Chemistry an Asian Journal 2 Pages: 1174-1185
  • Peer Reviewed
• [Journal Article] DNA adduct formation in human hepatoma cells treated with 3-nitrobenzanthrone: analysis by the ^<32>P-postlabeling method (2007)
  • Author(s): Kanno, T., Kawanishi, M., Takamura-Enya, T., Arit, V. M., Phillips, D. H., Yagi. T.
  • Journal Title: Mutation Research 634 Pages: 184-191
  • Peer Reviewed
• [Journal Article] Identification of three major DNA adductsformed by the carcinogenic air pollutant 3-nitrobenzanthrone in rat lung at the C8 and N2 position of guanine and at the N6 position of adenine (2006)
  • Author(s): Arlt, V. M., Schmeiser, H. H., Osborne, M. R., Kawanishi, M., Kanno, T., Yagi, T.Phillips D. H. and Takamura-Enya, T.
  • Journal Title: Int. J. Cancer 118(9) Pages: 2139-2146
  • Peer Reviewed
• [Journal Article] 堺で観測した大気エアロゾル粒子の長期(1986~2004)観測結果 (2006)
  • Author(s): 伊藤憲男、溝畑朗
  • Journal Title: エアロゾル研究 21 Pages: 297-304
  • NAID: 10018792307
  • Peer Reviewed
• [Journal Article] Development of an in vitro blood-brain barrier model to study the effects of endosulfan on the permeability of tight junctions and a comparative study of the cytotoxic effects of endosulfan on rat and human glial and neuronal cell cultures (2006)
  • Author(s): Chan M.P., Morisawa, S., Nakayama, A., Kawamoto, Y., Yoneda, M.
  • Journal Title: Environ. Toxicol 21(3) Pages: 223-235
  • Peer Reviewed
• [Journal Article] A physiologically based pharmacokinetic model for endosulfan in the male Sprague-Dawley rats (2006)
  • Author(s): Chan, M.P., Morisawa, S, Nakayama, A., Kawamoto, Y., Sugimoto, M., Yoneda, M.
  • Journal Title: Environ. Toxicol 21(5) Pages: 464-478
  • Peer Reviewed
• [Journal Article] Identification of three major DNA adducts formed by the carcinogenic air pollutant 3-nitrobenzanthrone in rat lung at the C8 and N2 position of guanine and at the N6 position of adenine. (2006)
  • Author(s): Arlt, V. M.
  • Journal Title: International Journal of Cancer 118・9 Pages: 2139-2146
• [Journal Article] Establishment of Reporter Yeasts for Guinea Pig and Syrian Hamster Aryl Hydrocarbon Receptor Ligand Activity (2006)
  • Author(s): Kawanishi, M.
  • Journal Title: Genes and Environment 28・4 Pages: 167-172
  • NAID: 110005717027
• [Journal Article] Identification of three major DNA adducts formed by the carcinogenic air pollutant 3-nitrobenzanthrone in rat lung at the C8 and N2 position of guanine and at the N6 position of adenine. (2006)
  • Author(s): Arlt, V.M.
  • Journal Title: International Journal of Cancer in press
• [Journal Article] Translesion DNA synthesis across mono ADP-ribosylated dG by Y-family DNA polymerases. (2006)
  • Author(s): Kawanishi, M
  • Journal Title: Developments in Mutation Research in press
• [Presentation] 沿道藤粒子の性状と自動車排出寄与率の推移 (2009)
  • Author(s): 中島徹、溝畑朗
  • Organizer: 大気環境学会第47回大会
  • Place of Presentation: 東京
• [Presentation] 3-ニトロベンズアントロン-DNA付加体の損傷乗り越えと突然変異 (2008)
  • Author(s): 西田裕, 川西優喜, 高村岳樹, 八木孝司.
  • Organizer: 日本環境変異原学会第37回大会
  • Place of Presentation: 沖縄コンベンションセンター、那覇市
  • Year and Date: 2008-12-05
• [Presentation] 3-ニトロベンズアントロン-DNA 付加体の損傷乗り越えと突然変異 (2008)
  • Author(s): 西田裕, 川西優喜, 高村岳樹, 八木孝司
  • Organizer: 日本環境変異原学会第37回大会
  • Place of Presentation: 那覇市
• [Presentation] ヒト細胞を用いた、3, 6-Dinitrobenzo〔e〕pyreneの遺伝毒性評価 (2008)
  • Author(s): 萩尾宗一郎, 尾後早耶佳, 川西優喜, 渡辺徹志, 小田美光, 八木孝司
  • Organizer: 日本環境変異原学会第37回大会
  • Place of Presentation: 那覇市
• [Presentation] TCDDはHepG2細胞におけるベンゾ〔a〕ピレンのDNA付加体形成を抑制する (2008)
  • Author(s): 椎崎一宏, 川西優喜, 八木孝司
  • Organizer: 日本環境変異原学会第37回大会
  • Place of Presentation: 那覇市
• [Presentation] 大気汚染物質3-ニトロベンズアントロンによるDNA付加体の生成とTLS・突然変異 (2008)
  • Author(s): 川西優喜, 西田裕, 八木孝司
  • Organizer: 第31回日本分子生物学会・第81回日本生化学会合同年会
  • Place of Presentation: 神戸市
• [Presentation] 神経系培養細胞PC12における酢酸鉛のタンパク質発現変動 (2008)
  • Author(s): 中山亜紀, 友松千晴, 谷修介, 森澤眞輔, 大川克也, 米田稔
  • Organizer: 日本トキシコロジー学会第35回学術年会
  • Place of Presentation: 東京
• [Presentation] Consequence of Translesion DNA Synthesis across Site-specific 4-Aminobiphenyl Adducts in Human Cells (2007)
  • Author(s): Sawai T., Kawanishi ,M., Kanno T., Takamura T., Yagi, T.
  • Organizer: The 1st Asian Conference on Environmental Mutagens, and the 36th Annual Meeting of the Japanese Environmental Mutagens Society
  • Place of Presentation: Kitakyushu
• [Presentation] 硫酸塩エアロゾル粒子濃度変化の季節変化の推移 (2007)
  • Author(s): 伊藤憲男、溝畑朗
  • Organizer: 大気環境学会第48回大会
  • Place of Presentation: 岡山
• [Presentation] 堺での大気エアロゾル粒子の長期観測結果 (2007)
  • Author(s): 伊藤憲男、溝畑朗
  • Organizer: 大気環境学会第48回大会
  • Place of Presentation: 岡山
• [Presentation] Long-term continuous observation of the carbon component concentration in the atomospheric aerosol paryicles in Osaka (2007)
  • Author(s): Mizohata, A., Ito, N. and Matsumoto, J.
  • Organizer: Japan. Symposium on air quality measurement methods and technology by air and waste management association April
  • Place of Presentation: San Francisco, USA
• [Presentation] 鉛曝露による幼児・小児の健康リスク評価-PBPK モデルを適用した評価の試み- (2007)
  • Author(s): 森澤眞輔, 北神理紗子, 中山亜紀, 米田稔
  • Organizer: 日本リスク研究学会第20回研究発表会
  • Place of Presentation: 徳島
• [Presentation] 大腸菌における3-ニトロベンズアントロン-DNA付加体の損傷乗り越え合成 (2006)
  • Author(s): 西田裕, 川西優喜, 高村岳樹, 八木孝司
  • Organizer: 日本環境変異原学会第35回大会
  • Place of Presentation: 堺市
• [Presentation] 堺で観測した大気エアロゾル粒子の19年間(1986-2004)の濃度変化 (2006)
  • Author(s): 伊藤憲男、溝畑朗
  • Organizer: 大気環境学会第47回大会
  • Place of Presentation: 東京
• [Book] 大気と微粒子の話 (2008)
  • Author(s): 溝畑朗、笠原三紀夫・東野達
  • Publisher: 京都大学出版会
• [Book] 含炭素粒子測定と評価」笠原三紀夫・東野達(編)、エアロゾルの大気環境影響 (2007)
  • Author(s): 溝畑朗
  • Publisher: 京都大学出版会
• [Book] New Developments in Mutation Research (2006)
  • Author(s): Kawanishi, M
  • Total Pages: 272
  • Publisher: Nava Science Publishers