Repair mechanism of UV-induced DNA damage and roles of ubiquitination

• Project/Area Number: 17310040
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Risk sciences of radiation/Chemicals
• Research Institution: Kobe University (2007); The Institute of Physical and Chemical Research (2005-2006)
• Principal Investigator: SUGASAWA Kaoru Kobe University, Organization of Advanced Science ans Technology Biosignal Research Center, Professor (70202124)
• Project Period (FY): 2005 – 2007
• Project Status: Completed (Fiscal Year 2007)
• Budget Amount: ¥15,650,000 (Direct Cost: ¥14,300,000、Indirect Cost: ¥1,350,000); Fiscal Year 2007: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000); Fiscal Year 2006: ¥4,500,000 (Direct Cost: ¥4,500,000); Fiscal Year 2005: ¥5,300,000 (Direct Cost: ¥5,300,000)
• Keywords: nucleotide excision repair / xeroderma nigmentosum / DNA damage recognition / ubinuitination / XPC / UV-DDB / XPC複合体 / 脱ユビキチン化酵素

Research Abstract

Biochemical analyses were carried out on ubiquitylation of XPC and DDB2 by the UVDDB-ubiquitin hose (E3) complex induced in UV-irradiated cells. Upon ubiquitylation of DDB2, UVDDB lost its strong binding activity to UV-damaged DNA, whereas ubiquitylated XPC still retained its DNA binding activity. Furthermore, elongation of polyubiquitin chains seemed to be required for in vitro nucleotide excision repair (NER) reactions only when UV-DDB was present. Based on these results, we proposed a model concerning possible roles of UVDDB and ubiquitylation in the damage recognition process in NER.
In vivo dynamics of XPC were examined by the FRAP (flumesmnce recovely after photobleaching) technique using a stably transformed cell line expressing GFP-tagged XPC. We observed immobilization of GFPXPC after UV-irradiation of cells, which showed a biphasic UV-dose dependency. SiRNA knockdown and overexpression of DDB2 indicated that the first step immobilization observed after relatively low doses (5-10 J/m^2) of UV depended on UV-DDB. Our results suggest that the UV-DDB-mediated damage recognition pathway may be predominant when only a small number of UV photolesions are induced.
To investigate biological meanings of the reversible ubiquitylation XPC induced by UV-irradiation, we screened siRNA libraries for enzymes involved in deubiquitylation of XPC. A candidate enzyme has been obtained, which is now under investigation. In addition, both thymine DNA glycosylase (TDG) and XPC have been known to be SUMOylated. Structural basis of the functional modulation of TDG by SUMOylation could be revealed by the X-ray crystal structures of SUMOylated TDG. We also determined SUMOylation sites in XPC and mutations at these sites caused partial NER defects in vivo.

Research Products

• [Journal Article] Mutagenic radioadaptation in a human lymphoblastoid cell line. (2008)
  • Author(s): Yatagai. F., et. al.
  • Journal Title: Mutat. Res. 638 Pages: 48-55
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Xeroderma pigmentosum genes: functions inside and outside DNA repair. (2008)
  • Author(s): Sugasawa, K.
  • Journal Title: Carcinogenesis 29 Pages: 455-465
  • NAID: 120001348334
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Mutagenic radioadaptation in a human lymphoblastoid cell line (2008)
  • Author(s): Yatagai, F., Umehayashi, Y., Homma, M., Sugasawa, K., Takayama, Y., Hanaoka, F
  • Journal Title: Mutat. Res 638 Pages: 48-55
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Xeroderma pigmentosum genes: functions inside and outside DNA repair (2008)
  • Author(s): Sugasawa, K
  • Journal Title: Carcinogenesis 29 Pages: 455-465
  • NAID: 120001348334
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Xeroderma pigmentosum genes: functions inside and outside DNA repair. (2008)
  • Author(s): Kaoru Sugasawa
  • Journal Title: Carcinogenesis 29 Pages: 455-465
  • NAID: 120001348334
  • Peer Reviewed
• [Journal Article] In vivo destabilization and functional defects of the exroderma pigmentosum C protein caused by a pathogenic missense mutation. (2007)
  • Author(s): Yasuda, G., et. al.
  • Journal Title: Mol. Cell. Biol. 32 Pages: 6606-6614
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Sensing of DNA damage by XPC/Rad4: one protein for many lesions. (2007)
  • Author(s): Sugasawa, K, and Hanaoka, F.
  • Journal Title: Nat. Struct. Mol. Biol. 14 Pages: 887-888
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] In vivo destabilization and functional defects of the xeroderma pigmentosum C protein caused by a pathogenic missense mutation (2007)
  • Author(s): Yasuda, G., Nishi, R., Watanabe, E., Mori, T., lwai, S., Orioli, D., Stefanini, M., Hanaoka, F., Sugasawa, K
  • Journal Title: Mal. CelL BioL 27 Pages: 6606-6614
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Sensing of DNA damage by XPC/Rad4: one protein for many lesions (2007)
  • Author(s): Sugasawa, K., Hanaoka, F
  • Journal Title: Nat. Struct. Mot Biol 14 Pages: 887-888
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] In vivo destabilization and functional defects of the xeroderma pigmentosum C protein caused by a pathogenic missense mutation. (2007)
  • Author(s): Gentaro Yasuda, et. al.
  • Journal Title: Molecular and Cellular Biology 27 Pages: 6606-6614
  • Peer Reviewed
• [Journal Article] Sensing of DNA damage by XPC/Rad4: 0ne protein for many lesions. (2007)
  • Author(s): Kaoru Sugasawa and Fumio Hanaoka
  • Journal Title: Nature Structural and Molecular Biology 14 Pages: 887-888
• [Journal Article] Crystal structure of SUMO-3-modified thymine-DNA glycosylase. (2006)
  • Author(s): Baba, D., et. al.
  • Journal Title: J. Mol. Biol. 359 Pages: 137-147
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] The DNA repair-ubiquitin associated HR23 proteins are constituents of neuronal inclusions in specific neurodegencrative disorders without hampering DNA repair. (2006)
  • Author(s): Bergink, S., et. al.
  • Journal Title: Neurobiol. Dis. 23 Pages: 708-716
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] UV-induced ubiquitylation of XPC complex, the UV-DDB-ubiquitin ligase complex and DNA repair. (2006)
  • Author(s): Sugasawa, K.
  • Journal Title: J. Mol. Histol. 37 Pages: 189-202
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] The XPC complex and UV-DDB: functional assays for the damage recognition factors involved in global genome repair. (2006)
  • Author(s): Sugasawa, K.
  • Journal Title: Methods Enzymol. 408 Pages: 171-188
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Crystal structure of SUMO-3-modified thymine-DNA glycosylase (2006)
  • Author(s): Baba, D., Maita, N., Jce,J.-G., Uchimura, Y., Saitoh, H., Sugasawa, K., Hanaoka, F., Tochio, H., Hiroaki, H., and Shirakawa, M
  • Journal Title: J. MoL BioL 359 Pages: 137-147
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] The DNA repair-ubiquitin associated HR23 proteins are constituents of neuronal inclusions in specific neurodegenerative disorders without hampering DNA repair (2006)
  • Author(s): Bergink, S., Severijnen, L.-A., Wijgers, N., Sugasawa, K., Yousaf, H., Kros, J. M., van Swieten, J., Oostra, B. A., Hoeijmakers, J. H. J., Vermeulen, W., Willernsen, R
  • Journal Title: Neurobial Dis 23 Pages: 708-716
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] UV-induced ubiquitylation of XPC complex, the UV-DDB-ubiquitin ligase complex and DNA repair. (2006)
  • Author(s): Sugasawa, K
  • Journal Title: J. Mot Histot 37 Pages: 189-202
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] The DNA repair-ubiquitin associated HR23 proteins are constituents of neuronal inclusions in specific neurodegenerative disorders without hampering DNA repair (2006)
  • Author(s): Bergink, S. et al.
  • Journal Title: Neurobiology of Disease 23 Pages: 708-716
• [Journal Article] UV-induced ubiquitylation of XPC complex, the UV-DDB-ubiquitin ligase complex and DNA repair (2006)
  • Author(s): Sugasawa, K.
  • Journal Title: Journal of Molecular Histology 37 Pages: 189-202
• [Journal Article] ヌクレオチド除去修復における損傷認識機構とユビキチン化の役割 (2006)
  • Author(s): 菅澤 薫
  • Journal Title: 実験医学 24 Pages: 357-363
• [Journal Article] Cul4AリガーゼによるDNA修復経路 (2006)
  • Author(s): 菅澤 薫
  • Journal Title: 蛋白質核酸酵素 51 Pages: 1339-1344
• [Journal Article] UV-induced ubiquitylation of XPC protein mediated by UV-DDB-ubiquitin ligase complex. (2005)
  • Author(s): Sugasawa, K., et. al.
  • Journal Title: Cell 121 Pages: 387-400
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Crystal structure of thymine DNA glycosylase conjugated to SUMO-1. (2005)
  • Author(s): Baba, D., et. al.
  • Journal Title: Nature 435 Pages: 979-982
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Centrin 2 stimulates nucleotide excision repair by interacting with xeroderma pigmentosum group C protein. (2005)
  • Author(s): Nishi, R., et. al.
  • Journal Title: Mol. Cell. Biol. 25 Pages: 5664-5674
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Signal transducer and activator of transcription 3 is a key regulator of keratinocyte survival and proliferation following UV irradiation. (2005)
  • Author(s): Sano, S., et. al.
  • Journal Title: Cancer Res. 65 Pages: 5720-5729
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] UV-induced ubiquitylation of XPC protein mediated by UV-DDB-ubiquitin ligase complex (2005)
  • Author(s): Sugasawa, K., Okuda, Y., Saijo, M., Nishi, R., Matsuda, N., Chu, G., Mori, T., Iwai, S., Tanaka, K., Tanaka, K., Hanaoka, F
  • Journal Title: Cell 121 Pages: 387-400
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Crystal structure of thymine DNA glycosylase conjugated to SUMO-1 (2005)
  • Author(s): Baba, D., Maita, N., lee, J.-G., Uchimura, Y., Saitoh, H., Sugasawa, K., Hanaoka, Tochio, H., Hiroaki, H., Shirakawa, M
  • Journal Title: Nature 435 Pages: 979-982
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Centrin 2 stimulates nucleotide excision repair by interacting with xeroderma pigmentosum group C protein (2005)
  • Author(s): Nishi, R., Okuda, Y., Watanabe, E., Mori, T., lwai, S., Masutani, C., Sugasawa, K., Hanaoka, F
  • Journal Title: Mot Cell Biol 25 Pages: 5664-5674
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Signal transducer and activator of transcription 3 is a key regulator of keratinocyte survival and proliferation following UV irradiation (2005)
  • Author(s): Sano, S., Chan, K. S., Kira, M., Kalaoka, K., Takagi, S., Tarutani, M., Itami, S., Kiguchi, K., Yokoi, M., Sugasawa, K., Mori, T., Hanaoka, F., Takeda, J., DiGiovanni, J
  • Journal Title: Cancer Res 65 Pages: 5720-5729
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] UV-induced ubiquitylation of XPC protein mediated by UV-DDB-ubiquitin ligase complex (2005)
  • Author(s): Sugasawa, K. et al.
  • Journal Title: Gell 121 Pages: 387-400
• [Journal Article] Crystal structure of thymine DNA glycosylase conjugated to SUMO-1 (2005)
  • Author(s): Baba, D. et al.
  • Journal Title: Nature 435 Pages: 979-982
• [Journal Article] Centrin 2 stimulates nucleotide excision repair by interacting with xeroderma pigmentosum group C protein (2005)
  • Author(s): Nishi, R. et al.
  • Journal Title: Molecular and Cellular Biology 25 Pages: 5664-5674
• [Journal Article] Signal transducer and activator of transcription 3 is a key regulator of keratinocyte survival and proliferation following UV irradiation (2005)
  • Author(s): Sano, S. et al.
  • Journal Title: Cancer Research 65 Pages: 5720-5729
• [Presentation] ヌクレオチド除去修復因子の細胞内動態とその制御機構 (2008)
  • Author(s): 西良太郎, ほか
  • Organizer: 2007年度組換え・染色体再編ワークショップ・第19回DNA複製・分配ワークショップ
  • Place of Presentation: 伊豆
  • Year and Date: 2008-03-04
• [Presentation] ヌクレオチド除去修復因子の細胞内動態とその制御機構 (2008)
  • Author(s): 西良太郎, ほか
  • Organizer: 2007年度組換え・染色体再編ワークショップ・第19回DNA複製・分配ワークショップ・合同ワークショップ
  • Place of Presentation: 伊豆
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] In vivo dynamics and regulation of nucleotide excision repair factors (2008)
  • Author(s): Nishi, R., Alekseev, S., Dinant, C., Hoogstraten, D., Hoeijmakers, J. H. J., Vermeulen, W., Hanaoka, F., Sugasawa, K
  • Organizer: Workshop on DNA Recombination and Chromosomal Rearrangement 2007, and The 19th Workshop on DNA Replication and Segregation
  • Place of Presentation: Izu, Japan
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] ヌクレオチド除去修復因子の細胞内動態とその制御機構の解析 (2007)
  • Author(s): 西良太郎, ほか
  • Organizer: シンポジウム「DNA修復研究の最前線」.第30回日本分子生物学会年会・第80回日本生化学会大会(BMB2007)
  • Place of Presentation: 横浜
  • Year and Date: 2007-12-13
• [Presentation] ヌクレオチド除去修復における損傷認識とユビキチン化 (2007)
  • Author(s): 菅澤 薫, ほか
  • Organizer: ワークショップ「ユビキチン化を介したゲノムダイナミクスの制御」.第30回日本分子生物学会年会・第80回日本生化学会大会(BMB2007)
  • Place of Presentation: 横浜
  • Year and Date: 2007-12-13
• [Presentation] DNA修復因子と転写調節因子のSUMO化による活性調節の構造的基盤 (2007)
  • Author(s): 白川昌宏, ほか
  • Organizer: ワークショップ「ユビキチン化を介したゲノムダイナミクスの制御」.第30回日本分子生物学会年会・第80回日本生化学会大会(BMB2007)
  • Place of Presentation: 横浜
  • Year and Date: 2007-12-13
• [Presentation] ヌクレオチド除去修復におけるSUMO化の役割 (2007)
  • Author(s): 菅澤 薫
  • Organizer: 第5回SUMO研究集会
  • Place of Presentation: 京都
  • Year and Date: 2007-10-08
• [Presentation] Repair of UV-induced DNA damage: the molecular mechanism preventing skincancer. (2007)
  • Author(s): Kaoru Sugasawa, et. al.
  • Organizer: Symposium on "Genomic Instability" in The 66th Annual Meeting of the Japanese Cancer Association.
  • Place of Presentation: 横浜
  • Year and Date: 2007-10-03
• [Presentation] ヌクレオチド除去修復に関わるユビキチン化とSUMO化 (2007)
  • Author(s): 菅澤 薫
  • Organizer: 国立遺伝学研究所研究集会「ユビキチン・SUMOによるDNA複製およびDNA修復系の制御」
  • Place of Presentation: 三島
  • Year and Date: 2007-09-27
• [Presentation] Molecular mechanism promoting repair of UV-induced DNA damage. (2007)
  • Author(s): Kaoru Sugasawa
  • Organizer: The 3rd Japan-US DNA Repair Meeting
  • Place of Presentation: 仙台
  • Year and Date: 2007-05-08
• [Presentation] Molecular mechanism of nucleotide excision repair as a defense againstcancer. (2007)
  • Author(s): Kaoru Sugasawa
  • Organizer: International Conference on Cellular Responses to DNA Damage
  • Place of Presentation: Hsin-Chu, Taiwan
  • Year and Date: 2007-04-26
• [Presentation] Molecular mechanism of nucleotide excision repair as a defense against cancer. (2007)
  • Author(s): Sugasawa, K.
  • Organizer: International Conference on Cellular Responses to DNA Damage
  • Place of Presentation: Hsin-Chu, Taiwan
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Molecular mechanism promoting repair of UV-induced DNA damage (2007)
  • Author(s): Sugasawa, K.
  • Organizer: The 3rd Japan-US DNA Repair Meeting
  • Place of Presentation: Sendai, Japan
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Repair of UV-induced DNA damage: the molecular mechanism preventing skin cancer. (2007)
  • Author(s): Sugasawa, K., et. al.
  • Organizer: 第66回日本癌学会学術総会
  • Place of Presentation: 横浜
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] ヌクレオチド除去修復因子の細胞内動態とその制御機構の解析 (2007)
  • Author(s): 西良太郎, ほか
  • Organizer: 第30回日本分子生物学会年会・第80回日本生化学会大会(BMB2007)
  • Place of Presentation: 横浜
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] ヌクレオチド除去修復における損傷認識とユビキチン化 (2007)
  • Author(s): 菅澤 薫, ほか
  • Organizer: 第30回日本分子生物学会年会・第80回日本生化学会大会(BMB2007)
  • Place of Presentation: 横浜
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] DNA修復因子と転写調節因子のSUMO化による活性調節の構造的基盤 (2007)
  • Author(s): 白川昌宏, ほか
  • Organizer: 第30回日本分子生物学会年会・第80回日本生化学会大会(BMB2007)
  • Place of Presentation: 横浜
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Molecular mechanism of nucleotide excision repair as a defense against cancer (2007)
  • Author(s): Sugasawa, K
  • Organizer: International Conference on Cellular Responses to DNA Damage
  • Place of Presentation: Hsin-Chu, Taiwan
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Repair of UV-induced DNA damage: the molecular mechanism preventing skin cancer (2007)
  • Author(s): Sugasawa, K., Nishi, R. Hanaoka, F
  • Organizer: The 66th Annual Meeting of the Japanese Cancer Association
  • Place of Presentation: Yokohama, Japan
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] In vivo dynamics and regulation of nucleotide excision repair factors (2007)
  • Author(s): Nishi, R., Alekseev, S., Dinant, C., Hoogstraten, D., Hoeijmakers, J. H. J., Vermeulen, W., Hanabka, F., Sugasawa, K
  • Organizer: BMB2007
  • Place of Presentation: Yokohama, Japan
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Damage recognition and ubiquitylation in nucleotide excision repair (2007)
  • Author(s): Sugasawa, K., Shimura, T., Akagi, J., Nishi, R
  • Organizer: BMB2007
  • Place of Presentation: Yokohama, Japan
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Structural Basis of functional modulation by SUMO of DNA repair and transcription factors (2007)
  • Author(s): hirakawa, M., Baba, D., Arita, K., Tateishi, Y., Saitoh, H., Sugasawa, K., Nakai, A., Sekiyama, N., Tochio, T., Ariyoshi, M., Hiroaki, H
  • Organizer: BMB2007
  • Place of Presentation: Yokohama, Japan
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Roles of ubiquitylation in recognition of UV-induced DNA damage. (2006)
  • Author(s): Sugasawa, K., et. al.
  • Organizer: Meeting on DNA Repair: from Molecular Mechanism to Human Disease.
  • Place of Presentation: Noordwijkerhout, The Netherlands
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] A molecular mechanism underlying recognition of UV-induced DNA damage. (2006)
  • Author(s): Sugasawa, K., et. al.
  • Organizer: The 20th IUBMB International Congress of Biochemistry and Molecular Biology and the 11th FAOBMB Compress
  • Place of Presentation: Kyoto, Japan
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Biochemical studies on UV-DDB using nucleosomal DNA substrates. (2006)
  • Author(s): Yasuda, T., et. al.
  • Organizer: The 20th IUBMB International Congress of Biochemistry and Molecular Biology and the 11th FAOBMB Comgress
  • Place of Presentation: Kyoto, Japan
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] C群色素性乾皮症患者で発現するアミノ酸置換型変異XPCタンパク質の機能異常 (2006)
  • Author(s): 菅澤 薫, ほか
  • Organizer: 第65回日本癌学会学術総会
  • Place of Presentation: 横浜
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] ヌクレオチド除去修復に関わる損傷認識因子とその機能 (2006)
  • Author(s): 菅澤 薫, ほか
  • Organizer: 日本分子生物学会2006フォーラム
  • Place of Presentation: 名古屋
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Roles of ubiquitylation in recognition of UV-induced DNA damage (2006)
  • Author(s): Sugasawa, K., Okuda, Y., Saijo, M., Nishi, R., Matsuda, N., Tanaka, K., Tanaka, K., Hanaoka, F
  • Organizer: Meeting on DNA Repair: from Molecular Mechanism to Human Disease
  • Place of Presentation: Noordwijkerhout, The Netherlands
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] A molecular mechanism underlying recognition of UV-induced DNA damage (2006)
  • Author(s): Sugasawa, K., Okuda, Y., Saijo, M., Nishi, R., Matsuda, N., Tanaka, K., Tanaka, K., Hanaoka, F
  • Organizer: The 20th IUBMB International Congress of Biochemistry and Molecular Biology and the 11th FAOBMB Comgress
  • Place of Presentation: Kyoto, Japan
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Biochemical studies on UV-DDB using nucleosomal DNA substrates (2006)
  • Author(s): Yasuda, T., Sugasawa, K., Yasuda, G., Nishi, R., lwai, S., Shiomi, T., Hanaoka, F
  • Organizer: The 20th IUBMB International Congress of Biochemistry and Molecular Biology and the 11th FAOBMB Comgress
  • Place of Presentation: Kyoto, Japan
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Functional defects of a mutant XPCcontaining a pathogenic single amino acid substitution (2006)
  • Author(s): Sugasawa, K., Hanaoka, F
  • Organizer: The 65th Annual Meeting of the Japanese Cancer Association
  • Place of Presentation: Yokohama, Japan
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Damage recognition factors and their functions in nucleotide excision repair (2006)
  • Author(s): Sugasawa, K., Yasuda, G., Watanabe, E., Nishi, R., Hanaoka, F
  • Organizer: The Molecular Biology Society of Japan Forum 2006
  • Place of Presentation: Nagoya, Japan
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Roles of ubiquitylation in mammalian nucleotide excision repair. (2005)
  • Author(s): Sugasawa, K., et. al.
  • Organizer: The 58th Annual Meeting of the Japan Society for Cell Biology
  • Place of Presentation: Saitama, Japan
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Roles of ubiquitylation in mammalian nucleotide excision repair. (2005)
  • Author(s): Sugasawa, K.
  • Organizer: International Conference on Chemical Biology
  • Place of Presentation: Novosibirsk, Russia
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Ubiquitylation of damage recognition factors involved in nucleotide excision repair. (2005)
  • Author(s): Sugasawa, K., et. al.
  • Organizer: The 9th International Conference on Environmental Mutagens
  • Place of Presentation: San Francisco, U.S.A.
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] ヌクレオチド除去修復の損傷認識機構とユビキチン化の役割 (2005)
  • Author(s): 菅澤 薫, ほか
  • Organizer: 第64回日本癌学会学術総会
  • Place of Presentation: 札幌
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] アミノ酸置換型変異XPCタンパク質の機能異常の解析 (2005)
  • Author(s): 安田源太郎, ほか
  • Organizer: 日本遺伝学会第77回大会
  • Place of Presentation: 東京
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Roles of ubiquitylation in mammalian nucleotide excision repair. (2005)
  • Author(s): Sugasawa, K., et. al.
  • Organizer: 第78回日本生化学会大会
  • Place of Presentation: 神戸
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Recognition of UV-induced DNA damage and roles of ubiquitylation. (2005)
  • Author(s): Sugasawa, K.
  • Organizer: The 5th International Symposium on DNA Replication, Recombination and Repair(3R Symposium)
  • Place of Presentation: Awaji, Japan
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Recognition and repair of DNA base lesions for protection against cancer. (2005)
  • Author(s): Sugasawa, K., et. al.
  • Organizer: 日本環境変異原学会第34回大会
  • Place of Presentation: 東京
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] DNA塩基損傷の認識と修復機構 (2005)
  • Author(s): 菅澤 薫
  • Organizer: 生理学研究所研究会「DNA構造を基盤とするゲノム生理学の展開」
  • Place of Presentation: 岡崎
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] The XPC complex:damage recognition function and roles of subunits. (2005)
  • Author(s): Sugasawa, K.
  • Organizer: The 22nd Radiation Biology Center International Symposium
  • Place of Presentation: Kyoto, Japan
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] ヌクレオチド除去修復における損傷認識機構とユビキチン化の役割 (2005)
  • Author(s): 菅澤 薫, ほか
  • Organizer: 第28回日本分子生物学会年会
  • Place of Presentation: 福岡
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] DNA修復酵素TDGのSUMOによる構造・機能変換 (2005)
  • Author(s): 馬場大地, ほか
  • Organizer: 第28回日本分子生物学会年会
  • Place of Presentation: 福岡
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] UBL-UBAドメインの立体構造と分子認識 (2005)
  • Author(s): 白川昌宏, ほか
  • Organizer: 第28回日本分子生物学会年会
  • Place of Presentation: 福岡
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] 色素性乾皮症患者由来アミノ酸置換型変異XPCタンパク質の機能異常の解析 (2005)
  • Author(s): 安田源太郎, ほか
  • Organizer: 第28回日本分子生物学会年会
  • Place of Presentation: 福岡
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Roles of ubiquitylation in mammalian nucleotide excision repair (2005)
  • Author(s): Sugasawa, K., Okuda, Y., Saijo, M., Nishi, R., Matsuda, N., Tanaka, K., Tanaka, K., Hanaoka, F
  • Organizer: The 58th Annual Meeting of die Japan Society for Cell Biology
  • Place of Presentation: Saitama
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Roles of ubiquitylation in mammalian nucleotide excision repair (2005)
  • Author(s): Sugasawa, K
  • Organizer: International Conference on Chemical Biology Novosibirsk
  • Place of Presentation: Russia
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Ubiquitylation of damage recognition factors involved in nucleotide excision repair (2005)
  • Author(s): Sugasawa, K., Okuda, Y., Saijo, M., Nishi, R., Matsuda, N., Tanaka, K., Tanaka, K., Hanaoka, F
  • Organizer: The 9th International Conference on Environmental Mutagens San Francisco
  • Place of Presentation: U.S.A
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Damage recognition mechanism and roles of ubiquitylation in nucleotide excision repair (2005)
  • Author(s): Sugasawa, K., Saijo, M., Matsuda, N., Tanaka, K., Tanaka, K., Hanaoka, F
  • Organizer: The 64th Annual Meeting of the Japanese Cancer Association
  • Place of Presentation: Sapporo
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Functional defects of the mutant XPC protein harboring a single amino acid substitution (2005)
  • Author(s): Yasuda, G., Nishi, R., Watanabe, E., Mori, T., lwai, S., Sugasawa, K., Hanaoka, F
  • Organizer: The 77th Annual Meeting of the Genetics Society oflapan Tokyo
  • Place of Presentation: Japan
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Roles of ubiquitylation in mammalian nucleotide excision repair (2005)
  • Author(s): Sugasawa, K., Okuda, Y., Saijo, M., Nishi, R., Matsuda, N., Tanaka, K., Tanaka, K., Hanaoka, F
  • Organizer: The 78th Annual Meeting of the Japanese Biochemical Society Kobe
  • Place of Presentation: Japan
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Recognition of UV-induced DNA damage and roles of ubiquitylation (2005)
  • Author(s): Sugasawa, K
  • Organizer: The 5th International Symposium on DNA Replication, Recombination and Repair Awaji
  • Place of Presentation: Japan
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Recognition and repair of DNA base lesions for protection against cancer (2005)
  • Author(s): Sugasawa, K
  • Organizer: The 34th Annual Meeting of the Japanese Environmental Mutagen Society
  • Place of Presentation: Tokyo, Japan
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] The XPC complex: damage recognition function and roles of subunits (2005)
  • Author(s): Sugasawa, K
  • Organizer: The 22nd Radiation Biology Center International Symposium
  • Place of Presentation: Kyoto, Japan
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Damage recognition mechanism and roles of ubiquitylation in nucleotide excision repair (2005)
  • Author(s): Sugasawa, K., Okuda, Y., Saijo, M., Nishi, R., Matsuda, N., Tanaka, K., Tanaka, K., Hanaoka, F
  • Organizer: The 28th Annual Meeting of the Molecular Biology Society of Japan
  • Place of Presentation: Fukuoka, Japan
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Structural and functional modulation of DNA repair enzyme TDG by SUMO (2005)
  • Author(s): Baba, D., Maita, N., lee, J.-G., Uchimura, Y., Saitoh, H., Sueasawa, K., Hanaoka, F., Tochio, T., Hiroaki, H., Shirakawa, M
  • Organizer: The 28th Annual Meeting of the Molecular Biology Society oflapan
  • Place of Presentation: Fukuoka, Japan
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Structure and molecular recognition of the UBL-UBA domains (2005)
  • Author(s): Shirakawa, M., Fujiwara, K., Ohno, A., Kobayashi, H., Sugasawa, K., Hanaoka, F., Tenno, T., Tochio, T., Hiroaki, H
  • Organizer: The 28th Annual Meeting of the Molecular Biology Society oflapan
  • Place of Presentation: Fukuoka, Japan
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Functional defects of a mutant XPC containing a pathogenic single amino acid substitution (2005)
  • Author(s): Yasuda, G., Nishi, R., Watanabe, F., Mori, T., lwai, S., Sugasawa, K., Hanaoka, F
  • Organizer: The 28th Annual Meeting of the Molecular Biology Society ofJapan
  • Place of Presentation: Fukuoka, Japan
  • Description: 「研究成果報告書概要(欧文)」より
• [Book] Molecular Mechanisms of Xeroderma Pigmentosum (Ahmad, S. I. and Hanaoka, F. eds. ) (2008)
  • Author(s): Kaoru Sugasawa
  • Publisher: Landes Biosciences(In press)
• [Patent(Industrial Property Rights)] ヌクレオチド除去修復調節因子のスクリーニング方法 (2005)
  • Inventor(s): 菅澤 薫, ほか
  • Industrial Property Rights Holder: 独立行政法人理化学研究所
  • Industrial Property Number: 2005-120878
  • Filing Date: 2005-04-19
  • Description: 「研究成果報告書概要(和文)」より
• [Patent(Industrial Property Rights)] ヌクレオチド除去修復調節因子のスクリーニング方法 (2005)
  • Inventor(s): 菅澤 薫, 清水 友紀, 花岡 文雄
  • Industrial Property Rights Holder: 独立行政法人理化学研究所
  • Industrial Property Number: 2005-120878
  • Filing Date: 2005-04-19