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Development of Electrochemical Biolithograpy and application to Cellular Chips

Research Project

Project/Area Number 17310080
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Microdevices/Nanodevices
Research InstitutionTohoku University

Principal Investigator

NISHIZAWA Matsuhiko  Tohoku University, Graduate School of Engineering, Professor (20273592)

Co-Investigator(Kenkyū-buntansha) ABE Takashi  Graduate School of Engineering, 大学院・工学研究科, Associate Professor (00333857)
Project Period (FY) 2005 – 2007
Project Status Completed (Fiscal Year 2007)
Budget Amount *help
¥14,870,000 (Direct Cost: ¥14,000,000、Indirect Cost: ¥870,000)
Fiscal Year 2007: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2006: ¥5,000,000 (Direct Cost: ¥5,000,000)
Fiscal Year 2005: ¥6,100,000 (Direct Cost: ¥6,100,000)
KeywordsLithography / Cellular Chip / Microdevice / Electrochemistry / マイクロ・ナノデバイス / 細胞・組織 / マイクロ電極 / バイオアッセイ / バイオチップ / マイクロ流路
Research Abstract

Microfluidic systems have been widely investigated for biological applications and hold great promise in the development of diagnostic assays and bioreactors. The combination of the surface patterning techniques with the microfluidic systems paves the way to multi-functional, high-throughput, and cost-effective analysis, in which the "real-time" and "on-demand" micropatterning of bioelements within the microchannels would be essentially required since such delicate materials are unstable to desiccation, oxidation, and heat. However, most of the photolithography-based techniques are unable to be applied within the sealed microchannels.
In this research, we have developed a novel technique "electrochemical bio-lithography", which enables the localized immobilization of proteins and cells within 3D microstructures such as microfluidic channels. The principle of the technique is based on our finding that the albumin- or heparin-coated surfaces, initially anti biofouling, rapidly becomes protein- and cell-adhesive upon exposure to the reactive oxidizing agent such as hypobromous acid, which can be produced by the electrochemical oxidation of bromide ion in a biological buffer solution. Since this lithography can be conducted under typical physiological conditions, it enables the spatiotemporal control of cell adhesion and growth on substrates; it facilities the stepwise immobilization of multitype protein arrays and multiphenotype cell arrays. And importantly, this technique is simple enough to be integrated into the miniaturized and semi-closed systems such as microfluidic devices, indicating the possible "on-demand" immobilization of proteins and living cells just prior to use of the microfluidic biodevices. In addition, we have achieved rapid cellular arrangement in the semi-closed microfluidic channel by combining electrochemical bio-lithography with negative dielectrophoresis (DEP).

Report

(4 results)
  • 2007 Annual Research Report   Final Research Report Summary
  • 2006 Annual Research Report
  • 2005 Annual Research Report
  • Research Products

    (33 results)

All 2008 2007 2006 2005

All Journal Article (18 results) (of which Peer Reviewed: 8 results) Presentation (13 results) Book (1 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] Localized Immobilization of Proteins onto Microstructures within a Preassembled Microfluidic Device2008

    • Author(s)
      M. Hashimoto , et. al.
    • Journal Title

      Sensors and Actuators B 128

      Pages: 545-551

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Localized Immobilization of Proteins onto Microstructures within a Preassembled Microfluidic Device2008

    • Author(s)
      M. Hashimoto, et. al.
    • Journal Title

      Sensors and Actuators B 128

      Pages: 545-551

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Stepwise Formation of Patterned Cell Co-Cultures in Silicone Tubing2007

    • Author(s)
      H. Kaji , et. al.
    • Journal Title

      Biotechnol. Bioeng. 98

      Pages: 919-925

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Stepwise Formation of Patterned Cell Co-Cultures in Silicone Tubing2007

    • Author(s)
      H. Kaji et. al.
    • Journal Title

      Biotechnol. Bioeng. 98

      Pages: 919-925

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Localized Immobilization of Proteins onto Microstructures within a Preassembled Microfluidic Deevice2007

    • Author(s)
      M. hashimoto et. al.
    • Journal Title

      Sens. and Actu. B 128

      Pages: 277-285

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Biomaterial and Devices for Altanative to Animal Experiments2007

    • Author(s)
      H. Kaji
    • Journal Title

      CMC

      Pages: 277-285

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Stepwise Formation of Patterned Cell Co-Cultures in Silicone Tubing2007

    • Author(s)
      H. Kaji, et. al.
    • Journal Title

      Biotechnol. Bioeng. 98

      Pages: 919-925

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A Porous Membrane-Based Culture Substrate for Localized In-Situ Electroporation of Adherent Mammalian Cells2007

    • Author(s)
      T. Ishibashi, et. al.
    • Journal Title

      Sensors and Actuators B 128

      Pages: 5-11

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Patterning Cellular Motility Using an Electrochemical Technique and a Geometrically-Confined Environment2006

    • Author(s)
      H. Kaji
    • Journal Title

      Langmuir 22

      Pages: 10784-10787

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] On-Demand Patterning of Protein Matrices inside a Microfluidc Device2006

    • Author(s)
      H. Kaji
    • Journal Title

      Anal. Chem. 78

      Pages: 5469-5473

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Patterning Cellular Motility Using an Electrochemical Technique and a Geometrically-Confined Environment2006

    • Author(s)
      H. Kaji et. al.
    • Journal Title

      Langmuir 22

      Pages: 10784-10787

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] On-Demand Patterning of Protein Matrices inside a Microfluidc Device2006

    • Author(s)
      H. Kaji et. al.
    • Journal Title

      Anal. Chem. 78

      Pages: 5469-5473

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Patterning Cellular Motility Using an Electrochemical Technique and a Geometrically-Confined Environment2006

    • Author(s)
      H.Kaji
    • Journal Title

      Langmuir 22

      Pages: 10784-10787

    • Related Report
      2006 Annual Research Report
  • [Journal Article] On-Demand Patterning of Protein Matrices inside a Microfluidc Device2006

    • Author(s)
      H.Kaji
    • Journal Title

      Anal. Chem. 78

      Pages: 5469-5473

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Patterning the Surface Cytophobicity of an Albumin-Physisorbed Substrateby Electrochemical Means2005

    • Author(s)
      H. Kaji
    • Journal Title

      Langmuri 21

      Pages: 6966-6969

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Patterning the Surface Cytophobicity of an Albumin-Physisorbed Substrate by Electrochemical Means2005

    • Author(s)
      H. Kaji et. al.
    • Journal Title

      Langmuir 21

      Pages: 6966-6969

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Localized Chemical Stimulation of Cellular Micropatterns Using a Porous Membrane-Based Culture Substrate2005

    • Author(s)
      K.Takoh
    • Journal Title

      Sensors and Actuator B 108

      Pages: 683-687

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Patterning the Surface Cytophobicity of an Albumin-Physisorbed Substrate by Electrochemical Means2005

    • Author(s)
      H.Kaji
    • Journal Title

      Langmuir 21

      Pages: 6966-6969

    • Related Report
      2005 Annual Research Report
  • [Presentation] Arrangement of Cells within semi-Closed Space with Microelectrode-Based Technique2007

    • Author(s)
      M. Hashimoto , et. al.
    • Organizer
      Micro Total Analysis Systems 2007
    • Place of Presentation
      Paris
    • Year and Date
      2007-10-07
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
  • [Presentation] Generation of Patterned Cell Co-Cultures in Silicone Tubing Using a Microelectrode Technique and Electrostatic Assembly2007

    • Author(s)
      H. Kaji , et. al.
    • Organizer
      29th International Conference of the IEEE Engineering in Medicine and Biology Society
    • Place of Presentation
      Lyon
    • Year and Date
      2007-08-26
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
  • [Presentation] Generation of Patterned Cell Co-Cultures in Silicone Tubing Using a Microelectrode Technique and Electrostatic Assembly2007

    • Author(s)
      H. Kaji ed. al.
    • Organizer
      29th IEEE EMBS
    • Place of Presentation
      Lyon
    • Year and Date
      2007-08-26
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Electrochemical Biolithography for Micropatterning Proteins and Cells within Three-Dimensional Microstructures2007

    • Author(s)
      M. Nishizawa
    • Organizer
      MRS Spring Meeting
    • Place of Presentation
      San Francisco
    • Year and Date
      2007-04-10
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Integration of Antibody Arrays Into a Preassembled Microfluidic Devicefor On-Demand Immunoassay2006

    • Author(s)
      M. Hashimoto
    • Organizer
      Micro Total Analysis Systems 2006
    • Place of Presentation
      Tokyo
    • Year and Date
      2006-11-07
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Development of a Cell Culture system for Dynamically Controlling CellMotility2006

    • Author(s)
      H. Kaji
    • Organizer
      Micro Total Analysis Systems 2006
    • Place of Presentation
      Tokyo
    • Year and Date
      2006-11-07
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Integration of Antibody Arrays Into a Preassembled Microfluidic Device for On-Demand Immunoassay2006

    • Author(s)
      M. Hashimoto ed. al.
    • Organizer
      Micro Total Analysis Systems 2006
    • Place of Presentation
      Tokyo
    • Year and Date
      2006-11-07
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Development of a Cell Culture system for Dynamically Controlling Cell Motility2006

    • Author(s)
      H. Kaji ed. al.
    • Organizer
      Micro Total Analysis Systems 2006
    • Place of Presentation
      Tokyo
    • Year and Date
      2006-11-07
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] On-demand patterning of proteins inside a microfluidic device using an electrochemical method2006

    • Author(s)
      H. Kaji
    • Organizer
      The Ninth World Congress on Biosensors
    • Place of Presentation
      Toronto
    • Year and Date
      2006-05-11
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] On-demand patterning of proteins inside a microfluidic device using an electrochemical method2006

    • Author(s)
      H. Kaji ed. al.
    • Organizer
      The 9th World Congress on Biosensors
    • Place of Presentation
      Toronto
    • Year and Date
      2006-05-11
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Electrochemical Bio-Lithography for On-Demand Immobilization of Proteins and Cells inside Microfluidic Cannel2006

    • Author(s)
      M. Nishizawa
    • Organizer
      209th Meeting of The Electrochemical Society
    • Place of Presentation
      Denver
    • Year and Date
      2006-05-08
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Electrochemical Bio-Lithography for On-Demand Immobilization of Proteins and Cells inside Microfluidic Cannel2006

    • Author(s)
      M. Nishizawa ed. al.
    • Organizer
      209th Meeting of The ECS
    • Place of Presentation
      Denver
    • Year and Date
      2006-05-08
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Electrochemical Approach to Pattern Proteins and Cells within Microchannels2005

    • Author(s)
      H. Kaji
    • Organizer
      Micro Total Analysis Systems 2005
    • Place of Presentation
      Boston
    • Year and Date
      2005-10-12
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Book] 動物実験代替のためのバイオマテリアル・デバイス2007

    • Author(s)
      梶弘和
    • Publisher
      シーエムシー
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
  • [Patent(Industrial Property Rights)] タンパク質および細胞が内壁に固定された中空構造体2007

    • Inventor(s)
      梶弘和
    • Industrial Property Rights Holder
      東北大学
    • Industrial Property Number
      2007-022637
    • Filing Date
      2007-02-01
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary

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Published: 2005-04-01   Modified: 2016-04-21  

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