| Project/Area Number |
17310115
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
基礎ゲノム科学
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| Research Institution | Tokyo University of Science |
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Principal Investigator |
SHIMADA Hiroaki Tokyo University of Science, Department of Industrial Science, Professor (70281748)
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| Co-Investigator(Kenkyū-buntansha) |
KIKUCHI Shoshi Institute of Agrobiological Sciences, 農業生物資源研究所, Senior Researcher (90370650)
MATSUMOTO Ken'Ichiro Hokkaido University, Graduate School of Engineering, Assistant Professor (80360642)
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| Project Period (FY) |
2005 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥15,480,000 (Direct Cost: ¥14,400,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2007: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2006: ¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2005: ¥7,200,000 (Direct Cost: ¥7,200,000)
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| Keywords | rice / non-coding RNA / gene regulation / transgenic plants / control of translation / upstream ORF / 遺伝子発現調節 / 非飜訳RNA / 形質転換体 / 飜訳調節 / RNAi個体 / 発生・再分化 / 非コードRNA / アンチセンスRNA / 機能制御 / スプライシング / cDNA / 転写 |
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| Research Abstract |
The gene for a protein kinase, SPK, is divided into two parts. The region for the first part, corresponding to the exon 1, is independently expressed and may function as a non-coding RNA. We found a novel gene encoding an unidentified protein, OsMacl, on the opposite strand of the SPK exon 1 region, suggesting that the exon 1 RNA is a natural antisense RNA and may regulate this protein gene. The mRNA for OsMacl contained a long 5' untranslated sequence and there were several small ORFs, upstream ORF (uORF) , in this region. Since OsMacl was predicted to be localized in the cell membrane, this protein was suggested to be function as somewhat involved in external signals. The reported assay using the GUS gene revealed that the GUS gene downstream the uORFs of OsMacl was efficiently translated. These results suggest that there are some unknown regulation system using such uORFs.
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