| Budget Amount *help |
¥15,100,000 (Direct Cost: ¥15,100,000)
Fiscal Year 2006: ¥7,200,000 (Direct Cost: ¥7,200,000)
Fiscal Year 2005: ¥7,900,000 (Direct Cost: ¥7,900,000)
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| Research Abstract |
It is widely believed among researchers in the mouse genomics field that the random disruption of genes not expressed in the target cells is very difficult. We discovered that this difficulty is caused by an mRNA-surveillance mechanism, nonsense-mediated mRNA decay (NMD), developed a new gene-trap strategy called UPATrap, and overcome NMD-created obstacles in mouse genomics. We would be able to make a substantial contribution to the international collaborative effort of the knockout mouse project (KOMP) by randomly disrupting "dormant" genes in mouse ES cells with the use of our UPATrap technology.
Using the original UPATrap vector and mouse ES cells, we performed a medium-scale gene trapping, increased the number of the gene-trapped ES-cell clones, and made the information about the disrupted genes freely accessible to the outside researchers through the internet (http://bsw3.naist.jp/kawaichi/3kenfile/index.html). At the same time, we completed a new version of the UPATrap vector containing no IRES sequences for the suppression of NMD (unpublished data). In the meantime, one of the Canadian national genome projects, CMHD (http://www.cmhd.ca/), has employed our UPATrap technology, launched a collaboration with our group, and already disrupted a large number of genes in mouse ES cells using the gene-trap vector.
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